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Gene information
General information
TC011877 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12312125..12313825
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011877
    iBeetle screen iB_01899

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011877 RA
      MHRLLYIAG ALVLIGHVF CEIDHQIDV NNITNQLQE 
      NIPGLENFN ASKLPSEED VERMLKEKC EKNGGGEEA 
      FQALKNQQQ ELRTCLEAQ MNATQIQLE VEEAKKTGS 
      MDEIFGKYC RKYPEIYQC VEVVIGKVK PCLDEKEKD 
      TMNQTLKIL DELKEFVCF KDGDRIAMF VAEGGVECL 
      ESRKDELQQ CANQTLGSR IPTDMSATS LPVFLFTDR 
      ECNDFDKIR ACFNDELEK CKDSTPANI VDAFFKFLK 
      KHMPCNGVA GAKEAPSAV KESGGNSAG GLVVSTLIV 
      GVSVLISKF L
    CDS sequence
    • >TC011877 RA
      atgcaccga ctgctgtac atagctggg gccctggtc 
      ttaataggc cacgtgttt tgcgaaatc gaccaccaa 
      atcgacgtc aacaacatc accaaccag ctgcaggag 
      aacatcccc ggcctggag aatttcaac gcgagcaaa 
      ctgccctcc gaggaggac gtcgagcgc atgctgaag 
      gaaaagtgc gagaagaac ggcggcggc gaggaggct 
      ttccaggcc ctcaaaaac cagcaacaa gaactccgg 
      acgtgcctg gaggcccag atgaacgcc actcagatc 
      cagctggag gtggaggag gccaaaaaa accggttcc 
      atggacgaa atcttcggc aaatactgt cgcaagtac 
      ccggaaatc taccaatgc gtggaggtg gtgatcggg 
      aaagtgaag ccctgtctg gacgagaaa gagaaagac 
      acgatgaac caaacgttg aaaatcctg gacgagctc 
      aaggaattc gtctgtttc aaggacggc gaccgtatt 
      gccatgttt gtcgctgag ggcggtgtc gagtgtctg 
      gagtcccgg aaagacgag ctccagcag tgtgccaac 
      cagacgctg ggctcccgg atccccacc gacatgtcc 
      gccaccagc cttccggtc tttctcttc accgatcgg 
      gagtgcaac gactttgat aaaatccgc gcgtgtttc 
      aacgacgag ctggagaag tgcaaggac agcaccccg 
      gcaaacatc gtggatgcg ttcttcaag ttcttgaag 
      aagcacatg ccgtgcaat ggcgtcgcc ggggccaag 
      gaggcgccc agtgcggtc aaggaaagt ggcggaaat 
      tcagccgga ggtctggtg gtctccaca ctcatcgtc 
      ggggtctct gtcctgatc tcgaaattc ttgtga
    mRNA sequence
    • >TC011877 RA
      cattgctgg gaaaaccca acggtacaa tgatttttt 
      catatcata aaatcgcaa aattgcagt gaaaatcta 
      tcgcatttt tctcctcca aatccaatg tcagcggtc 
      tcaattaaa gccaagtgg ttcaaataa gtcatcaac 
      gatcaatgt tcgctttct gtatcaagg agataaagt 
      acaagtaga accaacagc gataaaagt gaaacggct 
      ccgagttgt tttagtcgt tccatgtcg ttcgcgttg 
      ctcgttcgt ttgaggtca tagcgagtc acctgtgta 
      aacccaaat aaaaatgca ccgactgct gtacatagc 
      tggggccct ggtcttaat aggccacgt gttttgcga 
      aatcgacca ccaaatcga cgtcaacaa catcaccaa 
      ccagctgca ggagaacat ccccggcct ggagaattt 
      caacgcgag caaactgcc ctccgagga ggacgtcga 
      gcgcatgct gaaggaaaa gtgcgagaa gaacggcgg 
      cggcgagga ggctttcca ggccctcaa aaaccagca 
      acaagaact ccggacgtg cctggaggc ccagatgaa 
      cgccactca gatccagct ggaggtgga ggaggccaa 
      aaaaaccgg ttccatgga cgaaatctt cggcaaata 
      ctgtcgcaa gtacccgga aatctacca atgcgtgga 
      ggtggtgat cgggaaagt gaagccctg tctggacga 
      gaaagagaa agacacgat gaaccaaac gttgaaaat 
      cctggacga gctcaagga attcgtctg tttcaagga 
      cggcgaccg tattgccat gtttgtcgc tgagggcgg 
      tgtcgagtg tctggagtc ccggaaaga cgagctcca 
      gcagtgtgc caaccagac gctgggctc ccggatccc 
      caccgacat gtccgccac cagccttcc ggtctttct 
      cttcaccga tcgggagtg caacgactt tgataaaat 
      ccgcgcgtg tttcaacga cgagctgga gaagtgcaa 
      ggacagcac cccggcaaa catcgtgga tgcgttctt 
      caagttctt gaagaagca catgccgtg caatggcgt 
      cgccggggc caaggaggc gcccagtgc ggtcaagga 
      aagtggcgg aaattcagc cggaggtct ggtggtctc 
      cacactcat cgtcggggt ctctgtcct gatctcgaa 
      attcttgtg attcgccaa aactttgtt atttattgt 
      tgttacacg tcttttagt agtgggtgc gtgactgtt 
      gccggaatg atatatttt tataaacaa tacgtaatg 
      tttttaaat aaataatct ttttttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY