Status
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Gene information
General information
TC034414
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011875,TC011874
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12330071..12343025
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034414
    iBeetle screen iB_01898

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 25.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Pupal morphology
    pronotum seta shortened - Number of animals showing the phenotype: 8

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034414 RA
      MNGTAIDDG STIDRRPLI TCAGELEVF STIEDNLSQ 
      ALPQDSCEW RRSLGRPVR SVHIGANFA PFSAAALPK 
      GAQWDLIRY PLFHIYWTE CTDVDLYKN TVRDDIENW 
      LKELQTRDI PDFLVVVVE NYDGKRTNK LLPRTTVLD 
      KIRTDFAPK QGDRCISVI NPGKVEARS ADSWRGLVS 
      RIRHLLLVA YARAVSRLE DHVRQQRER RNEIGWNFM 
      QYFHLQEEL AHVLEMLGV HEEALVQYD ELDALFSQF 
      VVNGITGES INWLGDFQK PLEQWHGLK INHSTMLSE 
      SPSLLELRA YLFAKQAQM LLKANKVWE MASRCLPFL 
      HLTTREITV LEINSPPGA VSCWLFLAC VEVLQVCDK 
      YNNADAVED YSLHTACLW AYASEKLRD LGELCGLMP 
      GGAPTSEQL HTVVGLSAG MGDNPGAPD SPSPTDKLK 
      EALCSQEAF KKTYLELAE LAMCTYKHI GRLRSARLV 
      GREVASFYL LLGETQTAA AFLSDAVRT FEQDKWHEL 
      AAQTQIELA ECYRKANDT KKFIKTSAS VAAALEIDT 
      LIRWSYFDE MRKCLQSLE HPLVVPFND IIKITSVAL 
      KNEAIILQD STINVELVI ESNFPREIL CTNVQLSIE 
      LDTKEGRKS EKYSKSRVL TGKDLKPRD PLLQRLQIF 
      RHLDLKQDK TLASASVVC KHPEHKRKD SAAAPPKSD 
      FQCCLQVAN LPLVVTPGV NVIRLSKQA TNVGKHYLG 
      QMAIFMKNL HLISQLLTP RVAFEVKEE APVVRLHKT 
      DAPLLSGFE QIMNLIITI GSYTVEPKS RIKLTASRG 
      LSFQTDPSE PLTTSLEIE LGERPPFSS TRTPLRVLA 
      DLLTKDHQV SISCPWTSK PIVVKLSFT PPLTTIWRL 
      NTVQHRKFV QINVTGQCE KDIYVEKPQ LSVGDFCKI 
      VDRNHSSSH CAFRLPESD TDFCATLRS AKPCRDAPP 
      HHSSGAPPR TSSPVDSIA FNSGASLRA EAEGGMNYS 
      ATRDAADSC WTKLYKRGE LRAAGSWNF SRRQTAENY 
      SKQVIKSPD IWTPIEIIH KWLCFKPLQ AEKML
    CDS sequence
    • >TC034414 RA
      atgaatggt acagcaatt gacgacggg tcaacgatt 
      gacaggcga ccactaatt acatgcgct ggcgagttg 
      gaagtcttt tcaacgatt gaagacaac ttgtctcaa 
      gccctgccg caggattcg tgcgagtgg cggcggtcg 
      ttgggtcgc cctgtgcgc tcggtgcac attggggcc 
      aatttcgcg ccctttagt gctgcggcc ctgcccaag 
      ggggcgcag tgggatctg atcaggtat cctttgttc 
      cacatttac tggacggag tgcacggac gtggatttg 
      tacaaaaat acagtcagg gatgatatt gagaattgg 
      ttgaaggag ttgcagacg cgggacatt cccgatttt 
      ttggtcgtt gtggtggaa aattacgat ggaaaacgt 
      actaataag ctgttaccg aggaccact gttttggat 
      aagatccgg acagatttt gctcccaaa caaggggat 
      cggtgcata tctgtgata aacccaggc aaagttgag 
      gcccgttct gctgactcc tggcggggt ttagtgagt 
      cgcattcgc cacttgttg ttggtggcg tatgcgcgt 
      gctgtatct cgccttgag gaccatgtg aggcaacaa 
      cgcgaacgt aggaacgaa attggttgg aattttatg 
      caatatttt cacctccaa gaggaattg gctcacgtt 
      ttagaaatg ttaggcgta cacgaggaa gctttagtg 
      caatacgac gaattagat gctctcttt tctcaattt 
      gtagtaaac ggaatcaca ggcgagtcg ataaactgg 
      cttggcgat ttccaaaag cctctagaa cagtggcat 
      ggacttaaa atcaatcac tcaacaatg ctctctgaa 
      agtccgtct ctgttagag ttgagagcg tatttgttt 
      gcaaagcag gctcaaatg ttgcttaaa gcaaataaa 
      gtgtgggag atggcttca aggtgtttg ccttttctt 
      catttaact acaagggaa ataacagtt ttagaaata 
      aattcgccg cctggtgcg gtctcttgt tggttgttt 
      ttggcctgt gttgaggtt ttacaggtt tgtgacaag 
      tacaataat gccgatgct gttgaagac tattccttg 
      catacggcc tgtttgtgg gcttatgca agtgaaaag 
      ttgcgcgat ttgggcgaa ttgtgcggc ctaatgcct 
      ggtggggcc cccacctct gagcaacta cacaccgtg 
      gtggggcta tcagctgga atgggcgat aatcccggg 
      gccccagac agcccttca cccacggac aagctcaaa 
      gaagcgtta tgttcgcag gaggctttc aaaaaaaca 
      tatttggag ttggcagaa ctggcaatg tgcacgtac 
      aaacacatc ggccgtctc cgctcggcc cgacttgtg 
      ggccgcgaa gtggcctcg ttttacctc cttttaggg 
      gaaacccaa acagctgcg gcattttta agcgacgca 
      gttcgcact ttcgaacag gacaaatgg cacgaattg 
      gccgctcaa acccaaatc gaactcgcc gaatgttac 
      cgaaaggcc aacgatacg aaaaaattc attaaaacg 
      agtgccagt gtggccgca gccttggaa atcgatact 
      ctgatccgt tggagctat tttgacgaa atgagaaag 
      tgtctccaa tcactcgaa catccccta gtagtgcct 
      ttcaacgat attatcaaa attacgagt gttgcccta 
      aagaacgaa gcgattatt ctgcaagac agcaccatc 
      aatgtggaa cttgtgatc gaatcgaac tttccgcgg 
      gagattctg tgcactaat gtgcaactg tcgatcgag 
      ttggacacg aaagagggc cgaaaaagc gaaaagtac 
      agcaaaagt cgcgtgttg actgggaaa gacttgaaa 
      ccgcgcgat cccttatta caaagactc caaattttt 
      cgacatttg gacctgaag caggacaaa acgttggcc 
      agtgcgagt gtggtttgt aagcatcca gagcacaag 
      aggaaggat agcgcggca gcgccgcct aagagcgat 
      tttcagtgt tgtctccaa gtcgccaat cttccgctt 
      gttgtcaca cccggtgtc aatgtgata agactgagt 
      aaacaggcg acgaacgtt gggaagcac tacttgggc 
      caaatggcc atttttatg aagaatctt cacttgatc 
      tcgcaattg ttgacgccg agagtggct ttcgaagtg 
      aaggaggag gcgcctgtt gtgaggctg cacaagacc 
      gatgctccg ttgttatca gggtttgag caaattatg 
      aatttgatt attacgatc gggagctat acagttgaa 
      ccaaagtct agaataaaa ctaactgct tctcggggt 
      ttaagtttc caaacagac cccagcgag cccctaacc 
      acaagttta gaaatcgaa ctcggggaa agaccccct 
      tttagtagt actcgaaca ccgttacga gtactcgcc 
      gatcttcta acaaaagat caccaagta tccattagt 
      tgtccttgg acgtctaaa ccaattgtt gttaagttg 
      agctttaca ccgcctttg acgacaatc tggcgatta 
      aatacagta caacatcgg aaatttgtc caaatcaat 
      gtgacagga caatgcgaa aaagacata tacgtagaa 
      aaaccccaa ctgagcgtg ggcgacttt tgtaagatt 
      gttgataga aatcatagt tctagtcat tgtgcgttc 
      cggttaccg gagtcggac acggatttc tgcgctacg 
      ctccggagt gcgaaaccg tgccgagac gctcctccc 
      caccattca tccggagcc ccaccacgg acttcgtca 
      cctgttgac agtattgca ttcaactcc ggggcgtct 
      ctgcgagcg gaagccgag ggtggcatg aattattca 
      gcgacgagg gacgctgcc gattcctgc tggacaaag 
      ttgtacaaa cgaggggaa ttacgcgcc gccggaagc 
      tggaatttt tcgcgccga caaacggcc gaaaactat 
      tctaaacaa gtaattaaa agtccagat atttggacg 
      ccgattgaa attattcac aagtggctg tgtttcaag 
      ccactgcag gcggaaaag atgctttaa
    mRNA sequence
    • >TC034414 RA
      cgtgaaatg acaacacgt tttaatttt tggggtgtt 
      taatcatct gtcaaactc atattgatg gtatttatt 
      ttagttgtg tgtgttaaa atcattaat gaaatacaa 
      tacgaacca tgaagatat caccccgcc cgccccttt 
      aaccgaaaa accacgcaa tagagtatc ttccgacaa 
      tgacctttt ttttgcctc ctggttaag tttcaaatt 
      ccaatcatg aatggtaca gcaattgac gacgggtca 
      acgattgac aggcgacca ctaattaca tgcgctggc 
      gagttggaa gtcttttca acgattgaa gacaacttg 
      tctcaagcc ctgccgcag gattcgtgc gagtggcgg 
      cggtcgttg ggtcgccct gtgcgctcg gtgcacatt 
      ggggccaat ttcgcgccc tttagtgct gcggccctg 
      cccaagggg gcgcagtgg gatctgatc aggtatcct 
      ttgttccac atttactgg acggagtgc acggacgtg 
      gatttgtac aaaaataca gtcagggat gatattgag 
      aattggttg aaggagttg cagacgcgg gacattccc 
      gattttttg gtcgttgtg gtggaaaat tacgatgga 
      aaacgtact aataagctg ttaccgagg accactgtt 
      ttggataag atccggaca gattttgct cccaaacaa 
      ggggatcgg tgcatatct gtgataaac ccaggcaaa 
      gttgaggcc cgttctgct gactcctgg cggggttta 
      gtgagtcgc attcgccac ttgttgttg gtggcgtat 
      gcgcgtgct gtatctcgc cttgaggac catgtgagg 
      caacaacgc gaacgtagg aacgaaatt ggttggaat 
      tttatgcaa tattttcac ctccaagag gaattggct 
      cacgtttta gaaatgtta ggcgtacac gaggaagct 
      ttagtgcaa tacgacgaa ttagatgct ctcttttct 
      caatttgta gtaaacgga atcacaggc gagtcgata 
      aactggctt ggcgatttc caaaagcct ctagaacag 
      tggcatgga cttaaaatc aatcactca acaatgctc 
      tctgaaagt ccgtctctg ttagagttg agagcgtat 
      ttgtttgca aagcaggct caaatgttg cttaaagca 
      aataaagtg tgggagatg gcttcaagg tgtttgcct 
      tttcttcat ttaactaca agggaaata acagtttta 
      gaaataaat tcgccgcct ggtgcggtc tcttgttgg 
      ttgtttttg gcctgtgtt gaggtttta caggtttgt 
      gacaagtac aataatgcc gatgctgtt gaagactat 
      tccttgcat acggcctgt ttgtgggct tatgcaagt 
      gaaaagttg cgcgatttg ggcgaattg tgcggccta 
      atgcctggt ggggccccc acctctgag caactacac 
      accgtggtg gggctatca gctggaatg ggcgataat 
      cccggggcc ccagacagc ccttcaccc acggacaag 
      ctcaaagaa gcgttatgt tcgcaggag gctttcaaa 
      aaaacatat ttggagttg gcagaactg gcaatgtgc 
      acgtacaaa cacatcggc cgtctccgc tcggcccga 
      cttgtgggc cgcgaagtg gcctcgttt tacctcctt 
      ttaggggaa acccaaaca gctgcggca tttttaagc 
      gacgcagtt cgcactttc gaacaggac aaatggcac 
      gaattggcc gctcaaacc caaatcgaa ctcgccgaa 
      tgttaccga aaggccaac gatacgaaa aaattcatt 
      aaaacgagt gccagtgtg gccgcagcc ttggaaatc 
      gatactctg atccgttgg agctatttt gacgaaatg 
      agaaagtgt ctccaatca ctcgaacat cccctagta 
      gtgcctttc aacgatatt atcaaaatt acgagtgtt 
      gccctaaag aacgaagcg attattctg caagacagc 
      accatcaat gtggaactt gtgatcgaa tcgaacttt 
      ccgcgggag attctgtgc actaatgtg caactgtcg 
      atcgagttg gacacgaaa gagggccga aaaagcgaa 
      aagtacagc aaaagtcgc gtgttgact gggaaagac 
      ttgaaaccg cgcgatccc ttattacaa agactccaa 
      atttttcga catttggac ctgaagcag gacaaaacg 
      ttggccagt gcgagtgtg gtttgtaag catccagag 
      cacaagagg aaggatagc gcggcagcg ccgcctaag 
      agcgatttt cagtgttgt ctccaagtc gccaatctt 
      ccgcttgtt gtcacaccc ggtgtcaat gtgataaga 
      ctgagtaaa caggcgacg aacgttggg aagcactac 
      ttgggccaa atggccatt tttatgaag aatcttcac 
      ttgatctcg caattgttg acgccgaga gtggctttc 
      gaagtgaag gaggaggcg cctgttgtg aggctgcac 
      aagaccgat gctccgttg ttatcaggg tttgagcaa 
      attatgaat ttgattatt acgatcggg agctataca 
      gttgaacca aagtctaga ataaaacta actgcttct 
      cggggttta agtttccaa acagacccc agcgagccc 
      ctaaccaca agtttagaa atcgaactc ggggaaaga 
      ccccctttt agtagtact cgaacaccg ttacgagta 
      ctcgccgat cttctaaca aaagatcac caagtatcc 
      attagttgt ccttggacg tctaaacca attgttgtt 
      aagttgagc tttacaccg cctttgacg acaatctgg 
      cgattaaat acagtacaa catcggaaa tttgtccaa 
      atcaatgtg acaggacaa tgcgaaaaa gacatatac 
      gtagaaaaa ccccaactg agcgtgggc gacttttgt 
      aagattgtt gatagaaat catagttct agtcattgt 
      gcgttccgg ttaccggag tcggacacg gatttctgc 
      gctacgctc cggagtgcg aaaccgtgc cgagacgct 
      cctccccac cattcatcc ggagcccca ccacggact 
      tcgtcacct gttgacagt attgcattc aactccggg 
      gcgtctctg cgagcggaa gccgagggt ggcatgaat 
      tattcagcg acgagggac gctgccgat tcctgctgg 
      acaaagttg tacaaacga ggggaatta cgcgccgcc 
      ggaagctgg aatttttcg cgccgacaa acggccgaa 
      aactattct aaacaagta attaaaagt ccagatatt 
      tggacgccg attgaaatt attcacaag tggctgtgt 
      ttcaagcca ctgcaggcg gaaaagatg ctttaatta 
      aaagttggg acatgaaaa agttcgctt tgaacgact 
      ttgcaaact tttaagtcg caaaaattc ataatcgag 
      actaaacaa gccaaatat tt
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