Status
Please wait ...
Gene information
General information
TC011857 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12522710..12528462
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011857
    iBeetle screen iB_01896

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011857 RA
      MDVPPPQEL TQPLQVPSK TLMGPGPSN CSPRVMEAI 
      SRPILGHMH PEIFKIMDD IKAGIRYVF QTSNPLTLA 
      VSASGHGGM EAVLCNLLE PGDTALIAV NGIWGERAA 
      SMAGRYGAK VTTVEAEVG HDFTLAQIE NAVSKHRPH 
      VLFVTQGES SSGVYQNIE GIGDICHRY GCLLAVDTV 
      ASLGGVPFL ADRWGVDAV YTGTQKVLG APAGLAPIS 
      FSPRAQRKI FERKSPVKV LYWDMTVLG DYWDCFGRS 
      RVYHHTIAS QLLYGLREG LAQIAEEGL ESVNRRHQE 
      CAQRFYRGL ERLRLQPFV TDTYKRLPT VTSIKVPDG 
      VDWREVVAY AMKNYSVEI SGGLGPSAN KVFRVGLMG 
      YNATPEKVD LVLEVLEKA LKASGWNRS KL
    CDS sequence
    • >TC011857 RA
      atggacgta ccaccgccc caagaacta acccagcct 
      ttgcaagtc ccctccaag accctcatg ggtccggga 
      ccctcaaac tgctcccca agggtcatg gaggccatt 
      tcgagaccc atcttgggg cacatgcat cccgaaata 
      ttcaagata atggacgat atcaaagcc ggcatccgc 
      tacgtgttc caaactagc aaccccctg accctcgcc 
      gtcagcgcc tccggtcac gggggcatg gaggccgtg 
      ctctgcaac ctcctggag cccggggac acggccctg 
      atcgccgtc aacggcatt tggggcgaa cgcgccgct 
      tccatggcc ggcagatac ggggccaaa gtcaccacc 
      gtcgaggcc gaagtcggt cacgatttc accctcgcc 
      cagatcgaa aatgccgtc agcaagcac cgaccacac 
      gtgcttttc gtcacccaa ggcgagagt tcctccggc 
      gtttatcaa aacatcgaa ggaattggc gatatctgc 
      cacagatat gggtgcctg ctggcggtg gacacggtg 
      gcctccttg ggcggagtg ccgttcctc gctgacagg 
      tggggcgtg gacgctgtc tacaccggc acgcagaag 
      gtcctaggc gctccggcc gggctcgcc cccatctcc 
      ttcagcccc agggcgcag cgtaaaatt ttcgagcgc 
      aagagcccg gtgaaggtg ctctactgg gacatgacg 
      gtgctgggc gattactgg gattgcttt ggtcgttcc 
      cgggtgtac catcacacc atcgcctcg cagctttta 
      tacggcctg agggagggt ttggcgcaa attgccgag 
      gaagggttg gagagcgtc aacagaagg caccaagag 
      tgcgctcag aggttttac cgggggttg gagaggttg 
      aggctgcaa cccttcgtc accgacacg tataagagg 
      ttgcctact gtgacgtcc atcaaagtg cctgacggt 
      gtggattgg cgggaagtg gtggcgtac gccatgaaa 
      aattattcg gtggaaatt agtggtggt ttgggcccg 
      tccgcgaat aaagtgttc agggttggt ttgatgggc 
      tataatgcg acgcctgag aaggttgat ctggttttg 
      gaggttttg gaaaaggcg ctgaaggcg tcaggttgg 
      aatcgcagt aaattgtaa
    mRNA sequence
    • >TC011857 RA
      gatttattg ctgggtgtg aagcgggct ttggcgact 
      caccaatgg ccaaatcca agtccacgc cggtttaaa 
      tcgcacatg acaccacta tttctcgca tttaaagct 
      cttcttcgt gcttgaaga ccggttgaa gacgcacac 
      tcgaaccga tgtctgcaa acaactgac tggcgaacc 
      agccgactt gatacgaga ccggcacga actcaacag 
      gtggccata ttgttgtct ggaagggag gtatacgat 
      tttttttct tttccttcc aaagttgta aaataaaat 
      ctgttgcgg cggcctctt ctcaactct ggaaattaa 
      ttgaatttg gccgacacg ctgcatcaa ggcggccga 
      gtgtgcgct gccaactgc aacactcgg tgtccctca 
      agtgataaa cgagcgatt gtttatgag caggaatat 
      gatactaat aaaaatttg tttatctga tcggatggc 
      tgaacgcgt acaaggaat ctcttcatt atacaagtg 
      ccgatgaat ggcgattag tcacgtgac gcgactgtc 
      acttgatta agtgacaca ggtatattg ttattttcg 
      cacaatagt tggcctgga gtgggcgtg tccggtgtg 
      tgccaatcg ggtcagcac tggattcaa attggccaa 
      ctgcgcgat cgccgccat gttgacaaa tatgtcaat 
      atgtttatg taattagag cgactttat ggcactttt 
      gagcgcttc catgtggtg taacaacca aatatttag 
      ggagcgcag cagtaaaaa ggttcacat caaaataaa 
      caaaggttt tgttaccgc ctccgagaa atactagca 
      taaattaca atcctatca ctcacgcct catcgggac 
      acaattatg tttttatta tttcaaaga ggcggaatt 
      atctcgata ttgtgtaaa gtcggaggg tcctatcgg 
      acccatctt aatgtattc tggcttaac ttatcagtt 
      ccatttgcg cccattcat tgaaatgac catgcatta 
      ataattttt ccacagttc gtcatcaaa ttctatact 
      tttaaacca aaccatacc aatgttaaa aggttaaac 
      cttgataaa gtcattatt ttgataagc ttgtaaata 
      aagagcgat catttacat gcaaactga tattacaag 
      ttatttctt ccttgagtt accatggac gtaccaccg 
      ccccaagaa ctaacccag cctttgcaa gtcccctcc 
      aagaccctc atgggtccg ggaccctca aactgctcc 
      ccaagggtc atggaggcc atttcgaga cccatcttg 
      gggcacatg catcccgaa atattcaag ataatggac 
      gatatcaaa gccggcatc cgctacgtg ttccaaact 
      agcaacccc ctgaccctc gccgtcagc gcctccggt 
      cacgggggc atggaggcc gtgctctgc aacctcctg 
      gagcccggg gacacggcc ctgatcgcc gtcaacggc 
      atttggggc gaacgcgcc gcttccatg gccggcaga 
      tacggggcc aaagtcacc accgtcgag gccgaagtc 
      ggtcacgat ttcaccctc gcccagatc gaaaatgcc 
      gtcagcaag caccgacca cacgtgctt ttcgtcacc 
      caaggcgag agttcctcc ggcgtttat caaaacatc 
      gaaggaatt ggcgatatc tgccacaga tatgggtgc 
      ctgctggcg gtggacacg gtggcctcc ttgggcgga 
      gtgccgttc ctcgctgac aggtggggc gtggacgct 
      gtctacacc ggcacgcag aaggtccta ggcgctccg 
      gccgggctc gcccccatc tccttcagc cccagggcg 
      cagcgtaaa attttcgag cgcaagagc ccggtgaag 
      gtgctctac tgggacatg acggtgctg ggcgattac 
      tgggattgc tttggtcgt tcccgggtg taccatcac 
      accatcgcc tcgcagctt ttatacggc ctgagggag 
      ggtttggcg caaattgcc gaggaaggg ttggagagc 
      gtcaacaga aggcaccaa gagtgcgct cagaggttt 
      taccggggg ttggagagg ttgaggctg caacccttc 
      gtcaccgac acgtataag aggttgcct actgtgacg 
      tccatcaaa gtgcctgac ggtgtggat tggcgggaa 
      gtggtggcg tacgccatg aaaaattat tcggtggaa 
      attagtggt ggtttgggc ccgtccgcg aataaagtg 
      ttcagggtt ggtttgatg ggctataat gcgacgcct 
      gagaaggtt gatctggtt ttggaggtt ttggaaaag 
      gcgctgaag gcgtcaggt tggaatcgc agtaaattg 
      taaatgaaa atggcgggt gtttgtgaa gcgtttatt 
      tctagctag caggtacac ttatatgta tagcttgga 
      ttgttacat gttgcatta ttaccacgt ccatgtcct 
      atttaatgt caacacaag aaataaaaa ttaccaaat 
      gcaatcttg aggccctct ttatcacta tattacaat 
      cttatggat acaaaagtt agttggata ataataaaa 
      gtattatta cgtc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY