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Gene information
General information
TC034423
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011855,TC011854
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid67: 4726781..4730069
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034423
    iBeetle screen iB_01895
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    head present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034423 RA
      MSKQLDVKS EYKKTAKRF SPKEYLLLG AGPTNISER 
      VAKALSHQC LNPMGADIF QIQDEIKVM LKYVFQTKN 
      NLTLVSQTS GNGGNEAIL VNLLDPGDR VVIAIGGTW 
      GEKMVDMGQ RYNFDILPL RKNPGEIFT LQELEDAVV 
      RHKAKMLFV THGESTGGT LQPLEGLGA MCHKHQCLF 
      AVDAIVSIS VEPLFVDRW EIDAVSAGS QKAIGAPPG 
      TCMLSFSPR AEKRMSEIK VPPPYYMDM KRLAVYWKC 
      TPEPRQYHY TFSSNLLAS VREALAEIC EEGLVEVWE 
      RHASNTRLF WKKLQEIGL EPFVQNVKN RFMGVTPVK 
      LPPGIDQME FLNFMRQKY QIDITPGYG PTLGKAIRV 
      GFLGQNSRP EMVEYMAEA LKGALEHFN NPEYTMSKQ 
      LDIECEYKK TAKRFSSPE YLLLGAGPM NISERVAKA 
      LSRQPLNPL GADIYQIQD EIKVMLKYL FQTKNNYTF 
      VSQTSGNGG NEAIMVNLL DPGDRVVIA IGGTWGEKM 
      VDMAQRYNF DILSLRKNP GEIFTLQEL ENAVVDHKA 
      KMLFVTHGE STGGTLQPL EGLGAMCHK YDCLLAVDA 
      IVSIAVDPL FVDRWEIDA VSAGSQKGI GAPPGTCML 
      SFSPRAEKR MSEIKVPPP YYLDMRRIA IYWKCTPEA 
      RQYQYTFSS NLLASLREA LAEICEEGL VQIWERHTS 
      NTKLFWKKL EKLGLEPFV KDVKNRFMG VTPVKLPPG 
      IDQIEFLNF LRQKFQIDI TAGHGPTSG KAIRVGFLG 
      HNSRPEVVE YLAEALKAA LEHFKK
    CDS sequence
    • >TC034423 RA
      atgtcgaaa caactggac gtaaaaagt gagtataag 
      aaaaccgcg aagcggttt tccccaaag gagtatctc 
      ctgttgggc gccgggccc acgaatatc tctgaacgg 
      gtcgctaaa gccttgtcc catcaatgc ttgaatcca 
      atgggggct gacattttc cagattcaa gacgaaatc 
      aaagtcatg ctgaagtat gtcttccaa acgaaaaat 
      aatctcacg cttgtgagc caaacgtcg ggaaatggc 
      ggcaatgaa gccatcttg gtcaatctt ctagacccc 
      ggagatcgg gtggtgata gcaattggg ggcacttgg 
      ggcgagaaa atggtcgat atggggcaa agatacaat 
      ttcgatatt ctcccactc aggaaaaat cccggcgaa 
      atttttact ttacaagag cttgaagat gcagtggta 
      cgccacaag gccaaaatg ttatttgtg actcatgga 
      gaatccacc ggagggact ctccaaccc cttgaaggt 
      cttggtgcc atgtgtcac aaacaccag tgcttgttt 
      gcagtcgat gctatcgtt tccatatca gtggaacca 
      ctttttgtc gatcgctgg gagattgac gctgtgagt 
      gctggcagt cagaaagcc ataggtgca cctcctggc 
      acttgtatg ctaagtttc agtcccaga gccgagaaa 
      cgaatgagc gagataaaa gtaccacca ccgtactac 
      atggacatg aaaagactg gcagtttac tggaagtgc 
      actcctgaa ccaagacag taccactac actttttcc 
      tcaaattta ctagcttca gtacgggaa gctttggct 
      gaaatttgt gaggagggt cttgtcgag gtttgggaa 
      cgacacgcg tccaacacg cgacttttc tggaagaaa 
      ctccaggaa atcggtcta gaaccgttc gtccagaac 
      gtcaagaac cgtttcatg ggggttacc cccgtgaag 
      cttccccca ggaatcgac caaatggag ttcctcaac 
      tttatgcga caaaagtac caaatcgat attacaccg 
      ggctatggg cccactttg ggcaaagct atccgcgtc 
      ggctttcta ggccagaac agcaggccc gaaatggtc 
      gaatacatg gcagaagcg ctaaagggc gctctcgaa 
      cattttaac aatccagag tacaccatg tcaaaacaa 
      ttagatatc gaatgtgag tacaagaaa accgcgaaa 
      cggttttct tcaccagaa taccttcta ttgggtgct 
      ggaccgatg aatatttct gaaagagtt gccaaagcg 
      ttatcacgc caacctttg aatcctttg ggagcagac 
      atttaccaa attcaagat gaaatcaaa gtcatgctg 
      aagtacctt ttccaaaca aaaaataat tacactttt 
      gtgagccaa acgtcggga aatggcggg aatgaggca 
      ataatggtc aatcttcta gaccccgga gatcgggtg 
      gttatagca attgggggc acttggggc gagaaaatg 
      gtagatatg gcgcaaaga tacaatttc gatattctc 
      tcgctcagg aaaaatccc ggcgaaatt tttacttta 
      caagaactt gaaaatgca gtggttgac cacaaagcc 
      aaaatgtta tttgtgact catggagaa tccaccggg 
      gggactctc caacccctt gaaggtctc ggtgccatg 
      tgccacaaa tacgactgt ctgcttgct gtcgacgct 
      attgtctct atagcagtg gacccactt tttgtcgat 
      cgatgggag attgacgct gtgagtgct ggaagccaa 
      aaaggcata ggtgcacct cctggcact tgtatgctg 
      agtttcagt cccagagct gagaaacga atgagtgag 
      ataaaagta ccacccccg tactactta gatatgaga 
      agaatagca atttactgg aagtgcacc cctgaagct 
      agacaatac cagtacaca ttttcatca aacttgcta 
      gcttcacta cgagaagct ttggcggaa atttgtgaa 
      gaaggtctt gtccagatt tgggaaagg catacgtcc 
      aatactaaa cttttctgg aaaaaactt gaaaaactt 
      ggtcttgaa cctttcgtg aaggacgtc aaaaatcgc 
      ttcatgggg gtcacccct gtgaagcta cctccagga 
      atcgaccaa attgagttt ctcaacttt ttacgacaa 
      aaatttcag attgatatc accgccggt cacggccct 
      accagcggt aaggctatt cgggtgggc tttttaggc 
      cataacagt agacccgaa gtggtcgaa tacttggca 
      gaggctcta aaagccgct ctcgagcac tttaagaaa 
      tga
    mRNA sequence
    • >TC034423 RA
      ctttatcgg gtctatatt accctagaa ctcaaggtg 
      agaaaacag tccctggaa caatgtcga aacaactgg 
      acgtaaaaa gtgagtata agaaaaccg cgaagcggt 
      tttccccaa aggagtatc tcctgttgg gcgccgggc 
      ccacgaata tctctgaac gggtcgcta aagccttgt 
      cccatcaat gcttgaatc caatggggg ctgacattt 
      tccagattc aagacgaaa tcaaagtca tgctgaagt 
      atgtcttcc aaacgaaaa ataatctca cgcttgtga 
      gccaaacgt cgggaaatg gcggcaatg aagccatct 
      tggtcaatc ttctagacc ccggagatc gggtggtga 
      tagcaattg ggggcactt ggggcgaga aaatggtcg 
      atatggggc aaagataca atttcgata ttctcccac 
      tcaggaaaa atcccggcg aaattttta ctttacaag 
      agcttgaag atgcagtgg tacgccaca aggccaaaa 
      tgttatttg tgactcatg gagaatcca ccggaggga 
      ctctccaac cccttgaag gtcttggtg ccatgtgtc 
      acaaacacc agtgcttgt ttgcagtcg atgctatcg 
      tttccatat cagtggaac cactttttg tcgatcgct 
      gggagattg acgctgtga gtgctggca gtcagaaag 
      ccataggtg cacctcctg gcacttgta tgctaagtt 
      tcagtccca gagccgaga aacgaatga gcgagataa 
      aagtaccac caccgtact acatggaca tgaaaagac 
      tggcagttt actggaagt gcactcctg aaccaagac 
      agtaccact acacttttt cctcaaatt tactagctt 
      cagtacggg aagctttgg ctgaaattt gtgaggagg 
      gtcttgtcg aggtttggg aacgacacg cgtccaaca 
      cgcgacttt tctggaaga aactccagg aaatcggtc 
      tagaaccgt tcgtccaga acgtcaaga accgtttca 
      tgggggtta cccccgtga agcttcccc caggaatcg 
      accaaatgg agttcctca actttatgc gacaaaagt 
      accaaatcg atattacac cgggctatg ggcccactt 
      tgggcaaag ctatccgcg tcggctttc taggccaga 
      acagcaggc ccgaaatgg tcgaataca tggcagaag 
      cgctaaagg gcgctctcg aacatttta acaatccag 
      agtacacca tgtcaaaac aattagata tcgaatgtg 
      agtacaaga aaaccgcga aacggtttt cttcaccag 
      aataccttc tattgggtg ctggaccga tgaatattt 
      ctgaaagag ttgccaaag cgttatcac gccaacctt 
      tgaatcctt tgggagcag acatttacc aaattcaag 
      atgaaatca aagtcatgc tgaagtacc ttttccaaa 
      caaaaaata attacactt ttgtgagcc aaacgtcgg 
      gaaatggcg ggaatgagg caataatgg tcaatcttc 
      tagaccccg gagatcggg tggttatag caattgggg 
      gcacttggg gcgagaaaa tggtagata tggcgcaaa 
      gatacaatt tcgatattc tctcgctca ggaaaaatc 
      ccggcgaaa tttttactt tacaagaac ttgaaaatg 
      cagtggttg accacaaag ccaaaatgt tatttgtga 
      ctcatggag aatccaccg gggggactc tccaacccc 
      ttgaaggtc tcggtgcca tgtgccaca aatacgact 
      gtctgcttg ctgtcgacg ctattgtct ctatagcag 
      tggacccac tttttgtcg atcgatggg agattgacg 
      ctgtgagtg ctggaagcc aaaaaggca taggtgcac 
      ctcctggca cttgtatgc tgagtttca gtcccagag 
      ctgagaaac gaatgagtg agataaaag taccacccc 
      cgtactact tagatatga gaagaatag caatttact 
      ggaagtgca cccctgaag ctagacaat accagtaca 
      cattttcat caaacttgc tagcttcac tacgagaag 
      ctttggcgg aaatttgtg aagaaggtc ttgtccaga 
      tttgggaaa ggcatacgt ccaatacta aacttttct 
      ggaaaaaac ttgaaaaac ttggtcttg aacctttcg 
      tgaaggacg tcaaaaatc gcttcatgg gggtcaccc 
      ctgtgaagc tacctccag gaatcgacc aaattgagt 
      ttctcaact ttttacgac aaaaatttc agattgata 
      tcaccgccg gtcacggcc ctaccagcg gtaaggcta 
      ttcgggtgg gctttttag gccataaca gtagacccg 
      aagtggtcg aatacttgg cagaggctc taaaagccg 
      ctctcgagc actttaaga aatgatgtg ttatcaaat 
      atatccatt taaatata