Status
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Gene information
General information
TC034426
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011839,TC011838
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12698988..12710444
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034426
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01894

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034426 RA
      MSGQVPARY NYTNYDIYF GVVPVGTTV VKPYHIRNH 
      HNEVVSYLV TRNTRISPI YQVFDANKY ETKLDPGRE 
      LVLKISYSP NIPGTKNYD YFTINDSRQ NFYRLCLRG 
      ECSGPSVTT SIRHLNFHK TPNHKSVKQ IFTLSNTSE 
      CLARFQFDC IRDEQGAFA VDPVEGVIK PQEHVYIEV 
      RFCPKTFRF YSRQLACLI LYHEPILIN LFGVYSNID 
      YDLTQSPLR YYQLPYEET VSFAGYLSD SVTLKYVPP 
      PVSFTVNYL DFGRMLPES SCQTLTTSF TNHMDKPVE 
      VEWEQSELF RIYPEKLEI PAKMSALYE CRFRPSAKC 
      RLSSAIING LVTWIPTEE DNPFTINVP IPVSLRFIG 
      HSFPGKQAW IPSISIIPS TIILPLCLP NHPSWTTFM 
      IEAHGHLPV LFKLLPPRK TNFIMKPMV GLVRTFQII 
      VVQLESSST EPNSYIEKW NIQLNGHES SHPIFIKAQ 
      TDIPRVIVG YENCIKYEP MQPGTQTSL YVPLANVSC 
      CHIQYEFLT PEFFGVVTP RGVLAPNEE VFVTCWYQS 
      DLSQIGESN FSVNCRIRC IYKEKVLIG EPVDIPVTV 
      FVEVVYSEL CAFPSIFKC GQIEYGTTI STCFHIFNF 
      GYSNINFKL CHDHALKDV ALYPTFGRC GSREKLLVN 
      VQFTLTNLG KRTLHIGYT NRLNEFSEN AINEGTKTI 
      FRIDYDCIC ATLQITDLL EHNLGGLFS HSDLWRFLR 
      IDAINSALK ETNEGEISR VDLCVPDCE MSQKLYYLV 
      FVVKNVSNA HMRVALKRR KICDCEQRQ SEESLTFRI 
      KVYDCPHKD LFTLELANP LMPANSAQR LTLKIRHTL 
      PDLNTLSYI LEMGNERTI IFNISVNSI PPDCYALSV 
      YQLDYRLHL GFVYIGLRE APVFAFWLY NNTSKAVPF 
      KLDDLEITK LCNEDGFPV LKSLNSIGF VKAQSRAPL 
      LFKFWPIEA KCYQVVVPL MLGTNPATF QIVGYGTDQ 
      IKKSEFRDV ASLAQKPLY NNVVSLSVD RLVIEPLLV 
      ASSTERLFF IRNRDRNHR IQFRWKTCF IDGLVKVEP 
      LTSDGFLEA QEAQPVIVK ITSLCEPCN VRLLLCCEF 
      VDYDQLVVY NDSATLFNE RRRSSVKEF VISDEIDGK 
      EKENNELED IELPPKPEY FTITLPINV SVVGYSDRD 
      ICLSVESQM KQKPCDTVP INVDEFLAE YFKETEKKE 
      KPIRPPLQE ISQEEGKVK DDEPLIAEE LTKNVLEHL 
      TSDAVFCDY FREKVNIMK NEPNPLYSQ IKLDGKNRR 
      KELEKEAEK FFTQAKLSE IAEISKEAV VHSLEATFH 
      VGPFARKHN DGFVPHDL
    CDS sequence
    • >TC034426 RA
      atgagtggc caagttccg gcccgctac aattacacc 
      aactacgac atctacttc ggagtggtt cccgtcggc 
      accaccgtc gtcaaaccc taccacatc aggaaccac 
      cacaacgaa gtcgtctct tacctcgta accagaaac 
      acgcgcatc agtcccatc taccaagtc tttgacgcc 
      aacaaatac gaaaccaaa ctcgatccc ggccgagaa 
      ctggtgcta aaaatcagt tattccccc aacatccct 
      ggtaccaaa aactacgac tatttcaca atcaacgac 
      agtcgccag aacttctac cgcctgtgc ctcaggggc 
      gagtgctca ggcccatcg gtcacaacc tccatccga 
      cacttaaat ttccacaaa acgcccaac cacaaaagc 
      gtcaaacaa attttcaca cttagtaat acttcggaa 
      tgtttggca aggttccag ttcgactgt attagggac 
      gaacagggg gctttcgca gtggatccg gtcgaaggt 
      gttatcaaa ccgcaagaa catgtctac attgaagtg 
      aggttttgc cccaaaact ttcaggttt tactcgcgg 
      caattggct tgtctcatt ttatatcat gaaccgatt 
      ttgatcaac ttatttgga gtctactca aacattgac 
      tacgacttg acccagtca cctctcagg tactatcaa 
      cttccatac gaagaaact gtgagtttt gccggatat 
      ttgagtgat agtgtgacc ctcaaatac gtccctccg 
      cccgtttct ttcaccgtt aattacctc gattttggg 
      cgaatgttg ccggaaagc tcgtgtcaa actttaaca 
      actagtttc acaaatcat atggataag ccagttgaa 
      gttgagtgg gagcaaagc gaactgttt cgaatttat 
      ccggaaaaa cttgaaatt ccggctaaa atgtcggct 
      ttgtatgaa tgtaggttt cgtccaagt gccaaatgt 
      agactctca tcggccata attaatggt ttggtcacg 
      tggatcccc acggaggag gataatccc tttaccatc 
      aatgtccca atccctgtc tcccttcgt tttattgga 
      cacagtttt cccggaaag caggcctgg atcccgtcc 
      atttctatt attcccagt actatcatc cttccctta 
      tgtctgcca aatcaccct tcttggacc acatttatg 
      atagaggcc cacggccac ttgcccgtc cttttcaaa 
      ctgttacca cctcgcaaa acaaatttt ataatgaaa 
      ccaatggtt ggtttggtg cgaaccttt caaataatc 
      gtcgtccaa ctcgaaagc agttccacc gaaccaaac 
      tcatacatc gaaaaatgg aacatccag ctcaacggc 
      cacgaaagc tcgcaccca attttcatc aaagcccaa 
      actgacatc cccagagtt atagtaggc tacgaaaac 
      tgtatcaaa tacgaaccc atgcagcct ggaacccag 
      acaagtctt tatgttcct ttggccaat gtctcctgt 
      tgccacatc cagtatgaa tttttgacc ccggagttc 
      ttcggagtc gttactcca cggggtgtt ttggccccc 
      aatgaagaa gtgtttgtt acttgttgg taccagtcg 
      gatttaagt caaattggt gaaagtaat ttttcggtg 
      aattgtcgc attcggtgc atttataag gaaaaagtt 
      ctaattgga gaacctgtg gatattcct gttacggtt 
      tttgtagaa gtggtttat tcagagctg tgtgctttt 
      ccgagcatt ttcaagtgc ggccaaatc gaatatgga 
      accacaatc agcacttgt ttccacatt ttcaatttc 
      ggttactct aacattaat ttcaaacta tgccacgac 
      catgcctta aaagacgtt gctttgtac cccactttt 
      ggacgttgt ggctctcgc gaaaaactc ctagtcaac 
      gtccagttc actttaacc aacttggga aaacgaacc 
      ctccacata ggctacacg aaccgcctc aacgaattt 
      tccgaaaat gctataaac gagggaacg aaaaccatt 
      ttccgaatt gactacgac tgcatctgt gccacgctc 
      caaatcacc gacttgttg gaacacaat ttaggcggt 
      ttgttcagt cacagcgac ttgtggcga tttttgcga 
      atagacgca atcaattcg gcgttaaaa gaaacgaat 
      gaaggggag atatcacgc gtggatttg tgcgtccca 
      gactgtgaa atgtcccaa aagttgtac tatttggtt 
      tttgtcgtg aaaaacgtg tcaaacgcc cacatgaga 
      gtggctcta aaaaggcga aaaatctgc gattgtgag 
      caaaggcag tcggaggaa agcctcact ttcagaatt 
      aaagtctac gattgtccc cacaaggac ttattcacg 
      cttgaattg gccaatcct ttgatgccg gccaattcc 
      gcccagcgc ttgactctc aaaattaga cacacgctc 
      cctgaccta aacacgctc tcatatatt ctcgaaatg 
      ggcaatgag aggacaatc attttcaat atttccgtc 
      aacagtatc ccgcctgat tgctacgcc ttgtctgtt 
      tatcagctg gactatagg ttgcacctg ggctttgtg 
      tacatcggc ctgagggaa gccccggtc tttgccttc 
      tggttgtac aacaacacg agcaaagca gtgcctttc 
      aaactggac gatcttgaa atcacgaag ttgtgcaat 
      gaggacggc tttcccgtt ttaaagtct ctcaattcg 
      atcggcttt gtcaaagcg cagagtcgg gcgccgctt 
      ttgttcaag ttttggccg attgaagcc aaatgttac 
      caagttgta gtgcccttg atgctagga acaaaccct 
      gcgactttt caaatagtg ggttatggg accgatcaa 
      ataaaaaag agcgagttt agggacgtt gcaagtttg 
      gcccaaaaa ccgctctac aacaacgtt gtgagcctg 
      tcggtggat cgtctggtg attgagcca cttttggtg 
      gcaagttcg accgaacgc ttgtttttt atccgaaat 
      cgggaccga aatcaccga atccagttt cgctggaaa 
      acttgtttt attgacggt ttggtcaaa gtggagccg 
      ttgaccagt gacggtttt ttggaagcg caagaggcg 
      caacctgtg attgtcaaa attacgtcc ctttgtgaa 
      ccgtgtaat gttcgactg ttactttgt tgcgaattt 
      gttgattac gaccagttg gtggtatat aacgattct 
      gcgacgtta tttaatgag agacggaga agttctgtt 
      aaggaattc gtaatatcg gacgagatt gatgggaaa 
      gaaaaagaa aataatgaa cttgaggat attgagctg 
      ccaccaaaa cccgaatac tttaccatc actttacca 
      attaatgta agtgtggtt gggtattcg gaccgtgat 
      atttgtctt tctgtggag tcgcaaatg aagcaaaag 
      ccttgcgat acggtaccg attaacgtg gatgaattt 
      ctagcagag tatttcaaa gaaacggaa aaaaaggaa 
      aagccgatt aggcctccg ttacaagaa ataagccaa 
      gaagagggt aaagttaaa gatgatgaa cccttgatt 
      gcggaagaa ttgaccaaa aatgttttg gagcattta 
      acgagtgat gcggtcttc tgtgactat ttccgcgaa 
      aaagtcaac ataatgaaa aacgaaccc aaccccttg 
      tactcccaa atcaaactc gatggaaag aacagacga 
      aaggagttg gaaaaggaa gcagaaaaa ttctttact 
      caagctaaa ctgtcggaa attgccgaa atttccaaa 
      gaagcggtg gttcacagt ttggaggcg acatttcac 
      gtgggaccg tttgcaaga aaacacaac gatgggttc 
      gtaccacat gacctgtga
    mRNA sequence
    • >TC034426 RA
      aaatttcca ctccttgtc accgattat ttcaaatca 
      aataaatta gaaatatcc gtcaagttc ctgtcagct 
      gtaaaatga gtggccaag ttccggccc gctacaatt 
      acaccaact acgacatct acttcggag tggttcccg 
      tcggcacca ccgtcgtca aaccctacc acatcagga 
      accaccaca acgaagtcg tctcttacc tcgtaacca 
      gaaacacgc gcatcagtc ccatctacc aagtctttg 
      acgccaaca aatacgaaa ccaaactcg atcccggcc 
      gagaactgg tgctaaaaa tcagttatt cccccaaca 
      tccctggta ccaaaaact acgactatt tcacaatca 
      acgacagtc gccagaact tctaccgcc tgtgcctca 
      ggggcgagt gctcaggcc catcggtca caacctcca 
      tccgacact taaatttcc acaaaacgc ccaaccaca 
      aaagcgtca aacaaattt tcacactta gtaatactt 
      cggaatgtt tggcaaggt tccagttcg actgtatta 
      gggacgaac agggggctt tcgcagtgg atccggtcg 
      aaggtgtta tcaaaccgc aagaacatg tctacattg 
      aagtgaggt tttgcccca aaactttca ggttttact 
      cgcggcaat tggcttgtc tcattttat atcatgaac 
      cgattttga tcaacttat ttggagtct actcaaaca 
      ttgactacg acttgaccc agtcacctc tcaggtact 
      atcaacttc catacgaag aaactgtga gttttgccg 
      gatatttga gtgatagtg tgaccctca aatacgtcc 
      ctccgcccg tttctttca ccgttaatt acctcgatt 
      ttgggcgaa tgttgccgg aaagctcgt gtcaaactt 
      taacaacta gtttcacaa atcatatgg ataagccag 
      ttgaagttg agtgggagc aaagcgaac tgtttcgaa 
      tttatccgg aaaaacttg aaattccgg ctaaaatgt 
      cggctttgt atgaatgta ggtttcgtc caagtgcca 
      aatgtagac tctcatcgg ccataatta atggtttgg 
      tcacgtgga tccccacgg aggaggata atcccttta 
      ccatcaatg tcccaatcc ctgtctccc ttcgtttta 
      ttggacaca gttttcccg gaaagcagg cctggatcc 
      cgtccattt ctattattc ccagtacta tcatccttc 
      ccttatgtc tgccaaatc acccttctt ggaccacat 
      ttatgatag aggcccacg gccacttgc ccgtccttt 
      tcaaactgt taccacctc gcaaaacaa attttataa 
      tgaaaccaa tggttggtt tggtgcgaa cctttcaaa 
      taatcgtcg tccaactcg aaagcagtt ccaccgaac 
      caaactcat acatcgaaa aatggaaca tccagctca 
      acggccacg aaagctcgc acccaattt tcatcaaag 
      cccaaactg acatcccca gagttatag taggctacg 
      aaaactgta tcaaatacg aacccatgc agcctggaa 
      cccagacaa gtctttatg ttcctttgg ccaatgtct 
      cctgttgcc acatccagt atgaatttt tgaccccgg 
      agttcttcg gagtcgtta ctccacggg gtgttttgg 
      cccccaatg aagaagtgt ttgttactt gttggtacc 
      agtcggatt taagtcaaa ttggtgaaa gtaattttt 
      cggtgaatt gtcgcattc ggtgcattt ataaggaaa 
      aagttctaa ttggagaac ctgtggata ttcctgtta 
      cggtttttg tagaagtgg tttattcag agctgtgtg 
      cttttccga gcattttca agtgcggcc aaatcgaat 
      atggaacca caatcagca cttgtttcc acattttca 
      atttcggtt actctaaca ttaatttca aactatgcc 
      acgaccatg ccttaaaag acgttgctt tgtacccca 
      cttttggac gttgtggct ctcgcgaaa aactcctag 
      tcaacgtcc agttcactt taaccaact tgggaaaac 
      gaaccctcc acataggct acacgaacc gcctcaacg 
      aattttccg aaaatgcta taaacgagg gaacgaaaa 
      ccattttcc gaattgact acgactgca tctgtgcca 
      cgctccaaa tcaccgact tgttggaac acaatttag 
      gcggtttgt tcagtcaca gcgacttgt ggcgatttt 
      tgcgaatag acgcaatca attcggcgt taaaagaaa 
      cgaatgaag gggagatat cacgcgtgg atttgtgcg 
      tcccagact gtgaaatgt cccaaaagt tgtactatt 
      tggtttttg tcgtgaaaa acgtgtcaa acgcccaca 
      tgagagtgg ctctaaaaa ggcgaaaaa tctgcgatt 
      gtgagcaaa ggcagtcgg aggaaagcc tcactttca 
      gaattaaag tctacgatt gtccccaca aggacttat 
      tcacgcttg aattggcca atcctttga tgccggcca 
      attccgccc agcgcttga ctctcaaaa ttagacaca 
      cgctccctg acctaaaca cgctctcat atattctcg 
      aaatgggca atgagagga caatcattt tcaatattt 
      ccgtcaaca gtatcccgc ctgattgct acgccttgt 
      ctgtttatc agctggact ataggttgc acctgggct 
      ttgtgtaca tcggcctga gggaagccc cggtctttg 
      ccttctggt tgtacaaca acacgagca aagcagtgc 
      ctttcaaac tggacgatc ttgaaatca cgaagttgt 
      gcaatgagg acggctttc ccgttttaa agtctctca 
      attcgatcg gctttgtca aagcgcaga gtcgggcgc 
      cgcttttgt tcaagtttt ggccgattg aagccaaat 
      gttaccaag ttgtagtgc ccttgatgc taggaacaa 
      accctgcga cttttcaaa tagtgggtt atgggaccg 
      atcaaataa aaaagagcg agtttaggg acgttgcaa 
      gtttggccc aaaaaccgc tctacaaca acgttgtga 
      gcctgtcgg tggatcgtc tggtgattg agccacttt 
      tggtggcaa gttcgaccg aacgcttgt tttttatcc 
      gaaatcggg accgaaatc accgaatcc agtttcgct 
      ggaaaactt gttttattg acggtttgg tcaaagtgg 
      agccgttga ccagtgacg gttttttgg aagcgcaag 
      aggcgcaac ctgtgattg tcaaaatta cgtcccttt 
      gtgaaccgt gtaatgttc gactgttac tttgttgcg 
      aatttgttg attacgacc agttggtgg tatataacg 
      attctgcga cgttattta atgagagac ggagaagtt 
      ctgttaagg aattcgtaa tatcggacg agattgatg 
      ggaaagaaa aagaaaata atgaacttg aggatattg 
      agctgccac caaaacccg aatacttta ccatcactt 
      taccaatta atgtaagtg tggttgggt attcggacc 
      gtgatattt gtctttctg tggagtcgc aaatgaagc 
      aaaagcctt gcgatacgg taccgatta acgtggatg 
      aatttctag cagagtatt tcaaagaaa cggaaaaaa 
      aggaaaagc cgattaggc ctccgttac aagaaataa 
      gccaagaag agggtaaag ttaaagatg atgaaccct 
      tgattgcgg aagaattga ccaaaaatg ttttggagc 
      atttaacga gtgatgcgg tcttctgtg actatttcc 
      gcgaaaaag tcaacataa tgaaaaacg aacccaacc 
      ccttgtact cccaaatca aactcgatg gaaagaaca 
      gacgaaagg agttggaaa aggaagcag aaaaattct 
      ttactcaag ctaaactgt cggaaattg ccgaaattt 
      ccaaagaag cggtggttc acagtttgg aggcgacat 
      ttcacgtgg gaccgtttg caagaaaac acaacgatg 
      ggttcgtac cacatgacc tgtgatttg aaattaagt 
      tggccccac tgtaattcc aacttaatt tcaaagtca 
      tggaccact ttatttcgt tttttattg tttacaata 
      attatcttc gattcgccg aaaaacagc taagaactt 
      gtcgccctc gtttcgcgt cactttctg cccttcttc 
      agagctatc aaataactt gtccactac tgggtcacg 
      tttctgcaa ttaaataat caaagaaat g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY