| General information | |||
| TC034426
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 12698988..12710444 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC034426 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01894 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC034426 RA
MSGQVPARY NYTNYDIYF GVVPVGTTV VKPYHIRNH HNEVVSYLV TRNTRISPI YQVFDANKY ETKLDPGRE LVLKISYSP NIPGTKNYD YFTINDSRQ NFYRLCLRG ECSGPSVTT SIRHLNFHK TPNHKSVKQ IFTLSNTSE CLARFQFDC IRDEQGAFA VDPVEGVIK PQEHVYIEV RFCPKTFRF YSRQLACLI LYHEPILIN LFGVYSNID YDLTQSPLR YYQLPYEET VSFAGYLSD SVTLKYVPP PVSFTVNYL DFGRMLPES SCQTLTTSF TNHMDKPVE VEWEQSELF RIYPEKLEI PAKMSALYE CRFRPSAKC RLSSAIING LVTWIPTEE DNPFTINVP IPVSLRFIG HSFPGKQAW IPSISIIPS TIILPLCLP NHPSWTTFM IEAHGHLPV LFKLLPPRK TNFIMKPMV GLVRTFQII VVQLESSST EPNSYIEKW NIQLNGHES SHPIFIKAQ TDIPRVIVG YENCIKYEP MQPGTQTSL YVPLANVSC CHIQYEFLT PEFFGVVTP RGVLAPNEE VFVTCWYQS DLSQIGESN FSVNCRIRC IYKEKVLIG EPVDIPVTV FVEVVYSEL CAFPSIFKC GQIEYGTTI STCFHIFNF GYSNINFKL CHDHALKDV ALYPTFGRC GSREKLLVN VQFTLTNLG KRTLHIGYT NRLNEFSEN AINEGTKTI FRIDYDCIC ATLQITDLL EHNLGGLFS HSDLWRFLR IDAINSALK ETNEGEISR VDLCVPDCE MSQKLYYLV FVVKNVSNA HMRVALKRR KICDCEQRQ SEESLTFRI KVYDCPHKD LFTLELANP LMPANSAQR LTLKIRHTL PDLNTLSYI LEMGNERTI IFNISVNSI PPDCYALSV YQLDYRLHL GFVYIGLRE APVFAFWLY NNTSKAVPF KLDDLEITK LCNEDGFPV LKSLNSIGF VKAQSRAPL LFKFWPIEA KCYQVVVPL MLGTNPATF QIVGYGTDQ IKKSEFRDV ASLAQKPLY NNVVSLSVD RLVIEPLLV ASSTERLFF IRNRDRNHR IQFRWKTCF IDGLVKVEP LTSDGFLEA QEAQPVIVK ITSLCEPCN VRLLLCCEF VDYDQLVVY NDSATLFNE RRRSSVKEF VISDEIDGK EKENNELED IELPPKPEY FTITLPINV SVVGYSDRD ICLSVESQM KQKPCDTVP INVDEFLAE YFKETEKKE KPIRPPLQE ISQEEGKVK DDEPLIAEE LTKNVLEHL TSDAVFCDY FREKVNIMK NEPNPLYSQ IKLDGKNRR KELEKEAEK FFTQAKLSE IAEISKEAV VHSLEATFH VGPFARKHN DGFVPHDL
- >TC034426 RA
atgagtggc caagttccg gcccgctac aattacacc aactacgac atctacttc ggagtggtt cccgtcggc accaccgtc gtcaaaccc taccacatc aggaaccac cacaacgaa gtcgtctct tacctcgta accagaaac acgcgcatc agtcccatc taccaagtc tttgacgcc aacaaatac gaaaccaaa ctcgatccc ggccgagaa ctggtgcta aaaatcagt tattccccc aacatccct ggtaccaaa aactacgac tatttcaca atcaacgac agtcgccag aacttctac cgcctgtgc ctcaggggc gagtgctca ggcccatcg gtcacaacc tccatccga cacttaaat ttccacaaa acgcccaac cacaaaagc gtcaaacaa attttcaca cttagtaat acttcggaa tgtttggca aggttccag ttcgactgt attagggac gaacagggg gctttcgca gtggatccg gtcgaaggt gttatcaaa ccgcaagaa catgtctac attgaagtg aggttttgc cccaaaact ttcaggttt tactcgcgg caattggct tgtctcatt ttatatcat gaaccgatt ttgatcaac ttatttgga gtctactca aacattgac tacgacttg acccagtca cctctcagg tactatcaa cttccatac gaagaaact gtgagtttt gccggatat ttgagtgat agtgtgacc ctcaaatac gtccctccg cccgtttct ttcaccgtt aattacctc gattttggg cgaatgttg ccggaaagc tcgtgtcaa actttaaca actagtttc acaaatcat atggataag ccagttgaa gttgagtgg gagcaaagc gaactgttt cgaatttat ccggaaaaa cttgaaatt ccggctaaa atgtcggct ttgtatgaa tgtaggttt cgtccaagt gccaaatgt agactctca tcggccata attaatggt ttggtcacg tggatcccc acggaggag gataatccc tttaccatc aatgtccca atccctgtc tcccttcgt tttattgga cacagtttt cccggaaag caggcctgg atcccgtcc atttctatt attcccagt actatcatc cttccctta tgtctgcca aatcaccct tcttggacc acatttatg atagaggcc cacggccac ttgcccgtc cttttcaaa ctgttacca cctcgcaaa acaaatttt ataatgaaa ccaatggtt ggtttggtg cgaaccttt caaataatc gtcgtccaa ctcgaaagc agttccacc gaaccaaac tcatacatc gaaaaatgg aacatccag ctcaacggc cacgaaagc tcgcaccca attttcatc aaagcccaa actgacatc cccagagtt atagtaggc tacgaaaac tgtatcaaa tacgaaccc atgcagcct ggaacccag acaagtctt tatgttcct ttggccaat gtctcctgt tgccacatc cagtatgaa tttttgacc ccggagttc ttcggagtc gttactcca cggggtgtt ttggccccc aatgaagaa gtgtttgtt acttgttgg taccagtcg gatttaagt caaattggt gaaagtaat ttttcggtg aattgtcgc attcggtgc atttataag gaaaaagtt ctaattgga gaacctgtg gatattcct gttacggtt tttgtagaa gtggtttat tcagagctg tgtgctttt ccgagcatt ttcaagtgc ggccaaatc gaatatgga accacaatc agcacttgt ttccacatt ttcaatttc ggttactct aacattaat ttcaaacta tgccacgac catgcctta aaagacgtt gctttgtac cccactttt ggacgttgt ggctctcgc gaaaaactc ctagtcaac gtccagttc actttaacc aacttggga aaacgaacc ctccacata ggctacacg aaccgcctc aacgaattt tccgaaaat gctataaac gagggaacg aaaaccatt ttccgaatt gactacgac tgcatctgt gccacgctc caaatcacc gacttgttg gaacacaat ttaggcggt ttgttcagt cacagcgac ttgtggcga tttttgcga atagacgca atcaattcg gcgttaaaa gaaacgaat gaaggggag atatcacgc gtggatttg tgcgtccca gactgtgaa atgtcccaa aagttgtac tatttggtt tttgtcgtg aaaaacgtg tcaaacgcc cacatgaga gtggctcta aaaaggcga aaaatctgc gattgtgag caaaggcag tcggaggaa agcctcact ttcagaatt aaagtctac gattgtccc cacaaggac ttattcacg cttgaattg gccaatcct ttgatgccg gccaattcc gcccagcgc ttgactctc aaaattaga cacacgctc cctgaccta aacacgctc tcatatatt ctcgaaatg ggcaatgag aggacaatc attttcaat atttccgtc aacagtatc ccgcctgat tgctacgcc ttgtctgtt tatcagctg gactatagg ttgcacctg ggctttgtg tacatcggc ctgagggaa gccccggtc tttgccttc tggttgtac aacaacacg agcaaagca gtgcctttc aaactggac gatcttgaa atcacgaag ttgtgcaat gaggacggc tttcccgtt ttaaagtct ctcaattcg atcggcttt gtcaaagcg cagagtcgg gcgccgctt ttgttcaag ttttggccg attgaagcc aaatgttac caagttgta gtgcccttg atgctagga acaaaccct gcgactttt caaatagtg ggttatggg accgatcaa ataaaaaag agcgagttt agggacgtt gcaagtttg gcccaaaaa ccgctctac aacaacgtt gtgagcctg tcggtggat cgtctggtg attgagcca cttttggtg gcaagttcg accgaacgc ttgtttttt atccgaaat cgggaccga aatcaccga atccagttt cgctggaaa acttgtttt attgacggt ttggtcaaa gtggagccg ttgaccagt gacggtttt ttggaagcg caagaggcg caacctgtg attgtcaaa attacgtcc ctttgtgaa ccgtgtaat gttcgactg ttactttgt tgcgaattt gttgattac gaccagttg gtggtatat aacgattct gcgacgtta tttaatgag agacggaga agttctgtt aaggaattc gtaatatcg gacgagatt gatgggaaa gaaaaagaa aataatgaa cttgaggat attgagctg ccaccaaaa cccgaatac tttaccatc actttacca attaatgta agtgtggtt gggtattcg gaccgtgat atttgtctt tctgtggag tcgcaaatg aagcaaaag ccttgcgat acggtaccg attaacgtg gatgaattt ctagcagag tatttcaaa gaaacggaa aaaaaggaa aagccgatt aggcctccg ttacaagaa ataagccaa gaagagggt aaagttaaa gatgatgaa cccttgatt gcggaagaa ttgaccaaa aatgttttg gagcattta acgagtgat gcggtcttc tgtgactat ttccgcgaa aaagtcaac ataatgaaa aacgaaccc aaccccttg tactcccaa atcaaactc gatggaaag aacagacga aaggagttg gaaaaggaa gcagaaaaa ttctttact caagctaaa ctgtcggaa attgccgaa atttccaaa gaagcggtg gttcacagt ttggaggcg acatttcac gtgggaccg tttgcaaga aaacacaac gatgggttc gtaccacat gacctgtga
- >TC034426 RA
aaatttcca ctccttgtc accgattat ttcaaatca aataaatta gaaatatcc gtcaagttc ctgtcagct gtaaaatga gtggccaag ttccggccc gctacaatt acaccaact acgacatct acttcggag tggttcccg tcggcacca ccgtcgtca aaccctacc acatcagga accaccaca acgaagtcg tctcttacc tcgtaacca gaaacacgc gcatcagtc ccatctacc aagtctttg acgccaaca aatacgaaa ccaaactcg atcccggcc gagaactgg tgctaaaaa tcagttatt cccccaaca tccctggta ccaaaaact acgactatt tcacaatca acgacagtc gccagaact tctaccgcc tgtgcctca ggggcgagt gctcaggcc catcggtca caacctcca tccgacact taaatttcc acaaaacgc ccaaccaca aaagcgtca aacaaattt tcacactta gtaatactt cggaatgtt tggcaaggt tccagttcg actgtatta gggacgaac agggggctt tcgcagtgg atccggtcg aaggtgtta tcaaaccgc aagaacatg tctacattg aagtgaggt tttgcccca aaactttca ggttttact cgcggcaat tggcttgtc tcattttat atcatgaac cgattttga tcaacttat ttggagtct actcaaaca ttgactacg acttgaccc agtcacctc tcaggtact atcaacttc catacgaag aaactgtga gttttgccg gatatttga gtgatagtg tgaccctca aatacgtcc ctccgcccg tttctttca ccgttaatt acctcgatt ttgggcgaa tgttgccgg aaagctcgt gtcaaactt taacaacta gtttcacaa atcatatgg ataagccag ttgaagttg agtgggagc aaagcgaac tgtttcgaa tttatccgg aaaaacttg aaattccgg ctaaaatgt cggctttgt atgaatgta ggtttcgtc caagtgcca aatgtagac tctcatcgg ccataatta atggtttgg tcacgtgga tccccacgg aggaggata atcccttta ccatcaatg tcccaatcc ctgtctccc ttcgtttta ttggacaca gttttcccg gaaagcagg cctggatcc cgtccattt ctattattc ccagtacta tcatccttc ccttatgtc tgccaaatc acccttctt ggaccacat ttatgatag aggcccacg gccacttgc ccgtccttt tcaaactgt taccacctc gcaaaacaa attttataa tgaaaccaa tggttggtt tggtgcgaa cctttcaaa taatcgtcg tccaactcg aaagcagtt ccaccgaac caaactcat acatcgaaa aatggaaca tccagctca acggccacg aaagctcgc acccaattt tcatcaaag cccaaactg acatcccca gagttatag taggctacg aaaactgta tcaaatacg aacccatgc agcctggaa cccagacaa gtctttatg ttcctttgg ccaatgtct cctgttgcc acatccagt atgaatttt tgaccccgg agttcttcg gagtcgtta ctccacggg gtgttttgg cccccaatg aagaagtgt ttgttactt gttggtacc agtcggatt taagtcaaa ttggtgaaa gtaattttt cggtgaatt gtcgcattc ggtgcattt ataaggaaa aagttctaa ttggagaac ctgtggata ttcctgtta cggtttttg tagaagtgg tttattcag agctgtgtg cttttccga gcattttca agtgcggcc aaatcgaat atggaacca caatcagca cttgtttcc acattttca atttcggtt actctaaca ttaatttca aactatgcc acgaccatg ccttaaaag acgttgctt tgtacccca cttttggac gttgtggct ctcgcgaaa aactcctag tcaacgtcc agttcactt taaccaact tgggaaaac gaaccctcc acataggct acacgaacc gcctcaacg aattttccg aaaatgcta taaacgagg gaacgaaaa ccattttcc gaattgact acgactgca tctgtgcca cgctccaaa tcaccgact tgttggaac acaatttag gcggtttgt tcagtcaca gcgacttgt ggcgatttt tgcgaatag acgcaatca attcggcgt taaaagaaa cgaatgaag gggagatat cacgcgtgg atttgtgcg tcccagact gtgaaatgt cccaaaagt tgtactatt tggtttttg tcgtgaaaa acgtgtcaa acgcccaca tgagagtgg ctctaaaaa ggcgaaaaa tctgcgatt gtgagcaaa ggcagtcgg aggaaagcc tcactttca gaattaaag tctacgatt gtccccaca aggacttat tcacgcttg aattggcca atcctttga tgccggcca attccgccc agcgcttga ctctcaaaa ttagacaca cgctccctg acctaaaca cgctctcat atattctcg aaatgggca atgagagga caatcattt tcaatattt ccgtcaaca gtatcccgc ctgattgct acgccttgt ctgtttatc agctggact ataggttgc acctgggct ttgtgtaca tcggcctga gggaagccc cggtctttg ccttctggt tgtacaaca acacgagca aagcagtgc ctttcaaac tggacgatc ttgaaatca cgaagttgt gcaatgagg acggctttc ccgttttaa agtctctca attcgatcg gctttgtca aagcgcaga gtcgggcgc cgcttttgt tcaagtttt ggccgattg aagccaaat gttaccaag ttgtagtgc ccttgatgc taggaacaa accctgcga cttttcaaa tagtgggtt atgggaccg atcaaataa aaaagagcg agtttaggg acgttgcaa gtttggccc aaaaaccgc tctacaaca acgttgtga gcctgtcgg tggatcgtc tggtgattg agccacttt tggtggcaa gttcgaccg aacgcttgt tttttatcc gaaatcggg accgaaatc accgaatcc agtttcgct ggaaaactt gttttattg acggtttgg tcaaagtgg agccgttga ccagtgacg gttttttgg aagcgcaag aggcgcaac ctgtgattg tcaaaatta cgtcccttt gtgaaccgt gtaatgttc gactgttac tttgttgcg aatttgttg attacgacc agttggtgg tatataacg attctgcga cgttattta atgagagac ggagaagtt ctgttaagg aattcgtaa tatcggacg agattgatg ggaaagaaa aagaaaata atgaacttg aggatattg agctgccac caaaacccg aatacttta ccatcactt taccaatta atgtaagtg tggttgggt attcggacc gtgatattt gtctttctg tggagtcgc aaatgaagc aaaagcctt gcgatacgg taccgatta acgtggatg aatttctag cagagtatt tcaaagaaa cggaaaaaa aggaaaagc cgattaggc ctccgttac aagaaataa gccaagaag agggtaaag ttaaagatg atgaaccct tgattgcgg aagaattga ccaaaaatg ttttggagc atttaacga gtgatgcgg tcttctgtg actatttcc gcgaaaaag tcaacataa tgaaaaacg aacccaacc ccttgtact cccaaatca aactcgatg gaaagaaca gacgaaagg agttggaaa aggaagcag aaaaattct ttactcaag ctaaactgt cggaaattg ccgaaattt ccaaagaag cggtggttc acagtttgg aggcgacat ttcacgtgg gaccgtttg caagaaaac acaacgatg ggttcgtac cacatgacc tgtgatttg aaattaagt tggccccac tgtaattcc aacttaatt tcaaagtca tggaccact ttatttcgt tttttattg tttacaata attatcttc gattcgccg aaaaacagc taagaactt gtcgccctc gtttcgcgt cactttctg cccttcttc agagctatc aaataactt gtccactac tgggtcacg tttctgcaa ttaaataat caaagaaat g
