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Gene information
General information
TC011816 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid67: 5344707..5346245
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011816
    iBeetle screen iB_01891
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    egg chamber complete not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection



    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011816 RA
      MSKPGTSET QEQIAQKTW EMANKVELV NSVDDIYRY 
      DKKQQQDIL AAKPWEKDP HFFKDIKIS ALALLKMVM 
      HARSGGTLE VMGLILGKV DGNTMFVMD SFALPVEGT 
      ETRVNAQAQ AYEYMSSYI EAAKLVGRQ ENAIGWYHS 
      HPGYGCWLS GIDVGTQML NQNFQEPFV AIVIDPVRT 
      ISAGKVCLG AFRTYPKGY KPANEEPSE YQTIPLNKI 
      EDFGVHCKQ YYSLEVSYF KSSLDRRLL DSLWNKYWV 
      NTLSSSSLL TNADYTTGQ IFDLSEKLE QSEAAIGRG 
      GFIVGGTDP HEKRTEDKL LKATKDSCK TTIEIIHGL 
      MAQMIKDRL FNSTSLNNS TH
    CDS sequence
    • >TC011816 RA
      atgtcgaag ccggggacc tccgaaacc caggaacaa 
      atcgcacaa aaaacctgg gaaatggcc aataaagtc 
      gaactggtg aactcggtc gacgatatt taccgctac 
      gacaagaag cagcagcaa gacatcctc gccgcgaag 
      ccctgggag aaagaccct cacttcttc aaagatatc 
      aaaatctcg gccttggcc ctcttgaaa atggtcatg 
      cacgcgcgt tcggggggc accctggag gtcatgggc 
      ctgattttg ggcaaagtc gacggaaac acaatgttt 
      gtgatggac tcgtttgcg ctgcctgtg gaagggaca 
      gagacccga gtcaacgca caagctcag gcttacgag 
      tacatgagt tcgtacatc gaggctgcg aagctggtg 
      ggccgccag gagaacgcg ataggctgg taccacagc 
      cacccggga tacgggtgc tggttgtct gggattgac 
      gttggcacc cagatgctc aaccagaat ttccaggaa 
      ccgttcgtt gccattgtg attgatcct gtgagaacc 
      atttccgcg gggaaggtg tgtctaggg gcgttcagg 
      acgtacccg aaagggtac aaaccagca aatgaggaa 
      ccgtcagag taccagacc attcctctg aacaaaatc 
      gaggatttt ggggtgcat tgcaaacaa tattattcg 
      cttgaagtg tcgtatttt aaatcgtca cttgatagg 
      cgattgctt gattccttg tggaacaaa tattgggtc 
      aatactttg agctcgtct agtttgtta acgaatgct 
      gattacaca actgggcaa atttttgat ttgtcggag 
      aagttggaa cagtcggaa gcggccatt gggcgcggg 
      gggttcatc gtaggcggc actgatccg cacgaaaaa 
      cgaacagaa gacaaactg ttgaaagct acaaaggac 
      agttgcaag actacgatt gagattatt cacggactg 
      atggcccaa atgatcaaa gaccggctt tttaacagc 
      acttccttg aataattcg acccattaa
    mRNA sequence
    • >TC011816 RA
      acctgtccc accttgaaa aacacaatt cggtaaaaa 
      tagcccaat gaaggcaat aacgggcca ccaaatgcc 
      aagccactc gtaaaataa ttattttct gtgacaaaa 
      ctccttgtc ggtttgtgc cacaaacaa tttatcgat 
      tttttttca ttaccatta tgcattgtc tcattatta 
      cagcaatta atacgtaaa tcgtcccat atctaaaaa 
      aaaaaatta aaaaagtgt ccttccagt ttttatatt 
      tacgattgg tagcgctgt aatttgaca tctgtgttt 
      tgacaggtc cgatttttt cgaattttt gcccaaaat 
      agcccagaa ttacgcttt tgccccctt ttgccgcaa 
      tgtcgaagc cggggacct ccgaaaccc aggaacaaa 
      tcgcacaaa aaacctggg aaatggcca ataaagtcg 
      aactggtga actcggtcg acgatattt accgctacg 
      acaagaagc agcagcaag acatcctcg ccgcgaagc 
      cctgggaga aagaccctc acttcttca aagatatca 
      aaatctcgg ccttggccc tcttgaaaa tggtcatgc 
      acgcgcgtt cggggggca ccctggagg tcatgggcc 
      tgattttgg gcaaagtcg acggaaaca caatgtttg 
      tgatggact cgtttgcgc tgcctgtgg aagggacag 
      agacccgag tcaacgcac aagctcagg cttacgagt 
      acatgagtt cgtacatcg aggctgcga agctggtgg 
      gccgccagg agaacgcga taggctggt accacagcc 
      acccgggat acgggtgct ggttgtctg ggattgacg 
      ttggcaccc agatgctca accagaatt tccaggaac 
      cgttcgttg ccattgtga ttgatcctg tgagaacca 
      tttccgcgg ggaaggtgt gtctagggg cgttcagga 
      cgtacccga aagggtaca aaccagcaa atgaggaac 
      cgtcagagt accagacca ttcctctga acaaaatcg 
      aggattttg gggtgcatt gcaaacaat attattcgc 
      ttgaagtgt cgtatttta aatcgtcac ttgataggc 
      gattgcttg attccttgt ggaacaaat attgggtca 
      atactttga gctcgtcta gtttgttaa cgaatgctg 
      attacacaa ctgggcaaa tttttgatt tgtcggaga 
      agttggaac agtcggaag cggccattg ggcgcgggg 
      ggttcatcg taggcggca ctgatccgc acgaaaaac 
      gaacagaag acaaactgt tgaaagcta caaaggaca 
      gttgcaaga ctacgattg agattattc acggactga 
      tggcccaaa tgatcaaag accggcttt ttaacagca 
      cttccttga ataattcga cccattaac gcgttttaa 
      tctgtctga tttgtttgg atttaataa atatttatt 
      tataat