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Gene information
General information
TC011751 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid67: 6146676..6151512
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011751
    iBeetle screen iB_01886
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages sometimes not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen cuticle number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 16.67% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Pupal morphology
    head size decreased - Number of animals showing the phenotype: 7

    wings slightly orientation irregular - Number of animals showing the phenotype: 9

    wings slightly shortened - Number of animals showing the phenotype: 9
    Adult morphology
    head shape irregular - Number of animals showing the phenotype: 7

    Comment: pregnathal and gnathal head is somehow bent to the ventral side in relation to the postgnathal head

    gula shape irregular - Number of animals showing the phenotype: 7


    prothorax anterior rim bent - Number of animals showing the phenotype: 7


    elytra surface irregular - Number of animals showing the phenotype: 7


    elytra shape irregular - Number of animals showing the phenotype: 7

    metathoracic leg slightly shortened - Number of animals showing the phenotype: 7


    hindwing folding irregular - Number of animals showing the phenotype: 7

    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011751 RA
      MESLRKWFY RPKRDDKSL LAQFFFADE ALNIIANEL 
      DSFDGRKDP GRCTTLVNQ LRQSQDKVL TITNAIMDV 
      LIGDERANR DFRVKFPED VLQENLAGQ LWFGAECLA 
      AGSSIMNKE AESTAMRPL AKAVTKSLE TVRNLLRDT 
      CLRNNTPNG PIKLDSNEL VTEMLLESL KIFDKLFAE 
      FELAYVSAM VPVKSMQEY ELQELIGVL FSETLQRAL 
      KMKLLSQEM VDDCDPALM FTIPRLAIV SGLLIFPSG 
      PLCVDRPIE EMSNMFRPF RTLLHKIRE LLWTLNKRE 
      LFMLEKLLC DNEQISDVK SFSDSDFRY DFIDQFYGD 
      LHREEVVVV AKGEVSVNK DKPVVYPLE TDTPSTSGY 
      LIPNSIAHD IVVASVVEP QPGDSPPDH DSLEVISVA 
      AATLSSILA ENESPNDSG VCTENTSLD RSPSLDCQC 
      GVGCKCRKT SPRIKGKGK LPPTLPRKK RQVEDDASS 
      VGSSDTSSF NSNDDEEIA LAMQAAQIA EVRSKYRSS 
      EDLIHRLFV CIAGVADQL QTNFASDLR NILKSVFLM 
      NTSDAAVDT TSLEASLEY RPSEHDVIE NNEFSVDPN 
      ILAQEALFD TNVYFHLDP ESEDGEYTN HPRQEPSQA 
      NSSSERPPI WIPDVEAPK CMSCGMNFT VVKRRHHCR 
      NCGKVFCAR CSSNSVPLP KFGHHKPVR VCNKCFIYN 
      LTPFTM
    CDS sequence
    • >TC011751 RA
      atggagtcg ttgcggaag tggttctac agaccaaag 
      cgggatgac aaatctttg ttggcgcaa tttttcttc 
      gccgacgaa gctttgaac atcatcgcc aacgagttg 
      gacagtttt gatggacgc aaggacccg gggaggtgc 
      accacgttg gtcaaccag ctgaggcag agccaggac 
      aaggtgctg acgatcacc aatgccatc atggatgtg 
      ctcattggc gacgagagg gccaacagg gacttcagg 
      gtcaagttc ccggaggat gtgctccag gagaatttg 
      gcgggacag ctctggttt ggggctgag tgtttggcg 
      gccggctcg tccataatg aacaaagag gccgaaagc 
      acggccatg cgccccctc gccaaagcc gtcaccaag 
      agcttggag acggtgcgc aacctcctg cgcgacacc 
      tgcctccgc aacaacacc cccaacggc cccatcaag 
      ctcgacagc aacgagctc gtcaccgaa atgctcctc 
      gaaagttta aaaatcttc gataagtta ttcgccgag 
      tttgagctc gcctacgtc agcgccatg gtcccggtc 
      aagtccatg caagagtac gagctccag gagctgatc 
      ggcgtcctc ttcagcgag acgttgcaa cgcgcgctt 
      aaaatgaag ctcctgagc caggagatg gtcgacgat 
      tgcgacccg gcgctcatg tttactatt ccgcgactg 
      gcgatcgta agtggcctt ttgattttt ccaagtgga 
      cctttgtgc gtggacaga ccgatcgag gagatgagc 
      aatatgttt cggccgttt cggactttg ttgcacaag 
      atccgcgag ctgttgtgg acgttgaac aagcgcgag 
      ttgttcatg ttggagaag ttgttgtgc gataacgag 
      cagattagt gacgttaag tcgttcagt gatagtgat 
      tttcggtac gatttcatc gaccagttt tacggtgac 
      ttgcaccgc gaggaggtc gtggttgtg gccaagggg 
      gaggtgtcc gttaacaag gataaaccc gttgtttat 
      ccccttgaa acggacacg ccttcgact tcggggtac 
      ttgatcccg aatagtata gctcatgat atcgtggtg 
      gcgtcggtt gtggagccc cagcctggg gactcgccc 
      ccggatcac gactccctg gaggtgatc tcggtggcc 
      gcggccacg ctatcgtcg attttggcc gaaaacgag 
      tcaccgaac gattcgggc gtttgcacc gaaaacaca 
      agtcttgac cggtcacca agtctcgac tgtcaatgc 
      ggtgtgggc tgcaagtgc cggaaaacc tcccccagg 
      atcaaaggc aaaggcaaa ctcccaccg actttaccg 
      cggaagaag cgacaagtc gaagatgac gcttcaagt 
      gtcgggagc tcggacacc tcctcgttc aacagcaac 
      gacgatgag gagatcgcg ctggccatg caagcggct 
      cagatcgcc gaagtccgt tcgaagtac cgctcaagt 
      gaagacctc atccaccga ctcttcgtc tgcatcgct 
      ggagtggcc gaccagctc caaaccaac ttcgccagc 
      gacctgcgg aacatcctc aagtcggtt ttcctgatg 
      aacacctcg gacgcggcc gtggacacc acctccttg 
      gaggcgtcg ctggagtac cggccctcc gagcacgac 
      gtgatcgaa aacaacgaa ttctccgtc gacccgaac 
      atcctggcc caggaggcg cttttcgac accaacgtc 
      tacttccac ctggatccc gagtcggag gatggggag 
      tatacgaac catccgagg caggagccg agtcaggcg 
      aacagttcg agtgagcgg ccgccgatt tggatcccg 
      gacgtggag gcgcccaag tgcatgtcc tgcgggatg 
      aatttcacg gtggttaag cggaggcac cattgcagg 
      aattgcggg aaggttttc tgcgcgagg tgctcgtcg 
      aatagtgtt cctttgccc aagtttgga caccataag 
      cccgtgagg gtttgcaat aagtgtttt atttataat 
      ctgacgccg tttacgatg tga
    mRNA sequence
    • >TC011751 RA
      gcataattg gttgcataa tactataat tttttgtgt 
      cagcactac gtgcacacc tgtcaaaat cagtatgtc 
      gtgtgaatc attccagac gttttgttg tcttgaaac 
      gtgctaatc gcctcaaaa gttgataaa accgtagtt 
      ctaaactcc agagcaata acttggata atattttat 
      cgttctcca agcgccatg acgccaaaa gcagccttg 
      tctccgtgt cgtaaaagc ccattattt agtgcaaat 
      agtgatgat caacatggt ttctcttga aaccgtgct 
      ctaatttcc gcaaaagtt gcctataat tcgtcggaa 
      gcagcttgt ggacgatgc aggaagcct gaggatgga 
      gtcgttgcg gaagtggtt ctacagacc aaagcggga 
      tgacaaatc tttgttggc gcaattttt cttcgccga 
      cgaagcttt gaacatcat cgccaacga gttggacag 
      ttttgatgg acgcaagga cccggggag gtgcaccac 
      gttggtcaa ccagctgag gcagagcca ggacaaggt 
      gctgacgat caccaatgc catcatgga tgtgctcat 
      tggcgacga gagggccaa cagggactt cagggtcaa 
      gttcccgga ggatgtgct ccaggagaa tttggcggg 
      acagctctg gtttggggc tgagtgttt ggcggccgg 
      ctcgtccat aatgaacaa agaggccga aagcacggc 
      catgcgccc cctcgccaa agccgtcac caagagctt 
      ggagacggt gcgcaacct cctgcgcga cacctgcct 
      ccgcaacaa cacccccaa cggccccat caagctcga 
      cagcaacga gctcgtcac cgaaatgct cctcgaaag 
      tttaaaaat cttcgataa gttattcgc cgagtttga 
      gctcgccta cgtcagcgc catggtccc ggtcaagtc 
      catgcaaga gtacgagct ccaggagct gatcggcgt 
      cctcttcag cgagacgtt gcaacgcgc gcttaaaat 
      gaagctcct gagccagga gatggtcga cgattgcga 
      cccggcgct catgtttac tattccgcg actggcgat 
      cgtaagtgg ccttttgat ttttccaag tggaccttt 
      gtgcgtgga cagaccgat cgaggagat gagcaatat 
      gtttcggcc gtttcggac tttgttgca caagatccg 
      cgagctgtt gtggacgtt gaacaagcg cgagttgtt 
      catgttgga gaagttgtt gtgcgataa cgagcagat 
      tagtgacgt taagtcgtt cagtgatag tgattttcg 
      gtacgattt catcgacca gttttacgg tgacttgca 
      ccgcgagga ggtcgtggt tgtggccaa gggggaggt 
      gtccgttaa caaggataa acccgttgt ttatcccct 
      tgaaacgga cacgccttc gacttcggg gtacttgat 
      cccgaatag tatagctca tgatatcgt ggtggcgtc 
      ggttgtgga gccccagcc tggggactc gcccccgga 
      tcacgactc cctggaggt gatctcggt ggccgcggc 
      cacgctatc gtcgatttt ggccgaaaa cgagtcacc 
      gaacgattc gggcgtttg caccgaaaa cacaagtct 
      tgaccggtc accaagtct cgactgtca atgcggtgt 
      gggctgcaa gtgccggaa aacctcccc caggatcaa 
      aggcaaagg caaactccc accgacttt accgcggaa 
      gaagcgaca agtcgaaga tgacgcttc aagtgtcgg 
      gagctcgga cacctcctc gttcaacag caacgacga 
      tgaggagat cgcgctggc catgcaagc ggctcagat 
      cgccgaagt ccgttcgaa gtaccgctc aagtgaaga 
      cctcatcca ccgactctt cgtctgcat cgctggagt 
      ggccgacca gctccaaac caacttcgc cagcgacct 
      gcggaacat cctcaagtc ggttttcct gatgaacac 
      ctcggacgc ggccgtgga caccacctc cttggaggc 
      gtcgctgga gtaccggcc ctccgagca cgacgtgat 
      cgaaaacaa cgaattctc cgtcgaccc gaacatcct 
      ggcccagga ggcgctttt cgacaccaa cgtctactt 
      ccacctgga tcccgagtc ggaggatgg ggagtatac 
      gaaccatcc gaggcagga gccgagtca ggcgaacag 
      ttcgagtga gcggccgcc gatttggat cccggacgt 
      ggaggcgcc caagtgcat gtcctgcgg gatgaattt 
      cacggtggt taagcggag gcaccattg caggaattg 
      cgggaaggt tttctgcgc gaggtgctc gtcgaatag 
      tgttccttt gcccaagtt tggacacca taagcccgt 
      gagggtttg caataagtg ttttattta taatctgac 
      gccgtttac gatgtgagg agggtactt aatatttta 
      ggaaatttg aacggtttt ttgccttat ataattgta 
      ggatttatc gacgcattt gtggttttg acgacacgg 
      tttacttaa ttttctttt tctttaatg gagggttgg 
      atgccgcgc gatttttat gatgtgaga ttattttat 
      ttagtaagt acaaaaatt aaaagaaat gtttgtctc 
      aatagattt ttaatttgt tgtactgaa atattttgt 
      gataaatat tgtacattt tatttatat tgtggtttg 
      gggattatc catgtctct aataaaatt attttcaac 
      tg