Status
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Gene information
General information
TC011730 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14319557..14321075
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011730
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01885

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011730 RA
      MKRTFSSAA KTLVKKIQA RQIFDSRGN PTVEVDLVT 
      DLGLFRAAL PSGASTGVH EALELRDKD KANYHGKSV 
      FKAIKNVAL IAPEVIKSQ LCVTQQCQI DEIMVNLDG 
      TENKSKLGA NAILGVSLA VAKAGAATK KVSLFKHLA 
      DLAGNKDII LPVPAFNVI NGGSHAGNK LAMQEFMIL 
      PTGAKSFTE AMKMGSEVY HHLKNVIKA QFGLDATAV 
      GDEGGFAPN IQNNEDALN LIIEAIKKA GYEGKIDIG 
      MDIAASEFY KDGQYDLDF KNPKSDKSK WISGEKLLE 
      MYKGFIKKF PVVSIEDPF DQDDWTSWS AITSSTDIQ 
      IVGDDLTVT NPKRIQTAV EKKACNCLL LKVNQIGSV 
      TESIKAHLL AKSNGWGTM VSHRSGETE DTFIADLVV 
      GLSTGQIKT GAPCRSERL AKYNQILRI EEELGTKAK 
      YAGRNFKFP HK
    CDS sequence
    • >TC011730 RA
      atgaagcga acattctcc tcagcggct aaaacccta 
      gttaagaaa atccaagcg cgccaaatt tttgactct 
      cgtggtaac ccaactgtt gaagttgat ttagtcaca 
      gatttgggt cttttccga gcggctcta ccatctggt 
      gccagcact ggggttcac gaagctttg gagcttcga 
      gacaaagac aaagcgaac tatcacgga aaatcagtc 
      ttcaaagcg atcaaaaac gttgctctt atcgccccg 
      gaagtgata aaatcacaa ttatgtgta acccaacag 
      tgtcaaata gatgaaatt atggtaaac cttgacgga 
      actgagaac aagtccaag ctgggagca aacgcaatc 
      cttggggtg tcattagcc gtggccaaa gcaggcgca 
      gcgactaaa aaagtttcc ctgttcaaa catttggcc 
      gatttggcg gggaataaa gacataatc ctgcctgtt 
      ccggcgttt aatgtgata aatgggggc tctcatgcc 
      gggaacaag ctggcgatg caggagttt atgattttg 
      ccgacgggg gcgaagtcg ttcactgag gctatgaag 
      atggggagc gaggtctac caccacttg aagaacgtg 
      atcaaggcg cagttcggg ctggacgcc actgccgtg 
      ggggatgag ggcggcttt gcccccaac atccagaac 
      aacgaagac gcgcttaat ttgatcatc gaagcgatt 
      aaaaaagcc ggttatgag ggaaaaatc gacattggc 
      atggatata gccgcctct gagttttac aaagacggg 
      cagtacgat ttggacttc aaaaacccc aagtcagac 
      aagagcaaa tggatttcg ggggaaaaa ctcctcgag 
      atgtacaaa ggtttcatc aagaaattc ccggtggtg 
      tccattgag gatcccttc gaccaagac gactggacc 
      tcctggtcg gccatcacc tcctcgact gacatccag 
      atcgtcggg gatgacctg acggtgacc aaccccaag 
      cgcatccaa actgccgtt gagaagaag gcgtgcaat 
      tgccttttg ctcaaagtt aaccaaatc ggttcggtt 
      actgagtcg attaaagcg caccttttg gccaagtcc 
      aacggttgg gggacgatg gtctcgcac aggtctggc 
      gagactgaa gataccttc attgccgat ttagttgtg 
      ggactttcg acggggcaa attaaaaca ggagcccct 
      tgcaggtca gaacgtttg gctaagtat aaccagatt 
      ttgaggatt gaagaggaa ctaggaact aaagcgaaa 
      tacgcaggg cggaacttc aaattcccg cacaaataa
    mRNA sequence
    • >TC011730 RA
      cattattta agtgtgata atgaagcga acattctcc 
      tcagcggct aaaacccta gttaagaaa atccaagcg 
      cgccaaatt tttgactct cgtggtaac ccaactgtt 
      gaagttgat ttagtcaca gatttgggt cttttccga 
      gcggctcta ccatctggt gccagcact ggggttcac 
      gaagctttg gagcttcga gacaaagac aaagcgaac 
      tatcacgga aaatcagtc ttcaaagcg atcaaaaac 
      gttgctctt atcgccccg gaagtgata aaatcacaa 
      ttatgtgta acccaacag tgtcaaata gatgaaatt 
      atggtaaac cttgacgga actgagaac aagtccaag 
      ctgggagca aacgcaatc cttggggtg tcattagcc 
      gtggccaaa gcaggcgca gcgactaaa aaagtttcc 
      ctgttcaaa catttggcc gatttggcg gggaataaa 
      gacataatc ctgcctgtt ccggcgttt aatgtgata 
      aatgggggc tctcatgcc gggaacaag ctggcgatg 
      caggagttt atgattttg ccgacgggg gcgaagtcg 
      ttcactgag gctatgaag atggggagc gaggtctac 
      caccacttg aagaacgtg atcaaggcg cagttcggg 
      ctggacgcc actgccgtg ggggatgag ggcggcttt 
      gcccccaac atccagaac aacgaagac gcgcttaat 
      ttgatcatc gaagcgatt aaaaaagcc ggttatgag 
      ggaaaaatc gacattggc atggatata gccgcctct 
      gagttttac aaagacggg cagtacgat ttggacttc 
      aaaaacccc aagtcagac aagagcaaa tggatttcg 
      ggggaaaaa ctcctcgag atgtacaaa ggtttcatc 
      aagaaattc ccggtggtg tccattgag gatcccttc 
      gaccaagac gactggacc tcctggtcg gccatcacc 
      tcctcgact gacatccag atcgtcggg gatgacctg 
      acggtgacc aaccccaag cgcatccaa actgccgtt 
      gagaagaag gcgtgcaat tgccttttg ctcaaagtt 
      aaccaaatc ggttcggtt actgagtcg attaaagcg 
      caccttttg gccaagtcc aacggttgg gggacgatg 
      gtctcgcac aggtctggc gagactgaa gataccttc 
      attgccgat ttagttgtg ggactttcg acggggcaa 
      attaaaaca ggagcccct tgcaggtca gaacgtttg 
      gctaagtat aaccagatt ttgaggatt gaagaggaa 
      ctaggaact aaagcgaaa tacgcaggg cggaacttc 
      aaattcccg cacaaataa aacacaaat ctaataatc 
      ttggttgca aaaggatct atttgaaat ggtttaata 
      caattatta ttcaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY