Status
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Gene information
General information
TC011725 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14481684..14484351
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011725
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01884

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011725 RA
      MGNTVSAAE IAASQIVHP NTSLHQNAK KHEFHKTFT 
      HGEIPPECP MHRPKGASE CPVNHGEIN PLNMMGPAN 
      QNPSPGQPF PLPTERQVS NIPKAIVQE GEAPFWVYP 
      SQQMFWNAM LRKGWRWKD GDVTQKDMD DIIKIHNAN 
      NEQAWLEVL KWEALHAKE CGNPKLKSF GGKATTYSP 
      RARIRWLLG YELPFDRHD WIVDRCGKE VRYVIDYYD 
      GGMVDQKYQ FALLDVRPA MDSFENIWD RMTVAWWRW 
      RYGD
    CDS sequence
    • >TC011725 RA
      atggggaac acagtctca gcggccgaa atcgccgcc 
      tcccaaatc gtgcacccc aacactagt ctccaccag 
      aatgccaaa aagcacgag tttcacaaa accttcaca 
      cacggggaa ataccgccg gagtgtccc atgcaccgg 
      cccaagggg gcgtcggag tgtcccgtc aaccatggc 
      gagatcaac ccacttaac atgatgggg ccagccaac 
      cagaaccca tcgccggga caacccttc ccgctgccc 
      accgaaaga caagtctcc aacattccg aaagccatc 
      gtccaggag ggggaggcc cccttctgg gtgtacccc 
      agccaacaa atgttttgg aacgcaatg ttgcgtaaa 
      gggtggcgt tggaaggac ggggacgtt acgcaaaag 
      gacatggac gatattatc aaaatccac aacgcgaat 
      aacgagcag gcgtggctt gaagtccta aaatgggag 
      gcgctacat gccaaagag tgtggtaac ccaaaattg 
      aaaagtttt ggcgggaaa gcaaccact tacagccct 
      agagctcga attcgatgg ttgctaggt tacgaattg 
      ccatttgat cgccatgat tggattgtt gacaggtgt 
      ggcaaggaa gtgaggtat gtcatagac tactacgat 
      ggcggtatg gttgaccag aaatatcag tttgcgttg 
      ttggatgtg aggcctgct atggatagc tttgagaac 
      atttgggat aggatgact gtggcttgg tggcgatgg 
      cggtacggc gattaa
    mRNA sequence
    • >TC011725 RA
      gcaattccc ttaaatttg gaggataaa caaggatat 
      aattgcaag gataaaaaa agtccgcta ctagtgaat 
      taaaataaa ttacgtaca agccgtacg tagctttaa 
      ccacacatt gtgacataa tgccgggtc aaatttcat 
      caaatactt caaaaaagc agtaaaaat tcgccgttt 
      acaaattac cagtttcta gtgtctcct atttatcag 
      ttatttatt acataattt agatattac gctttttgc 
      ttcattacg taagtcgcc ctaacctca ctttatcaa 
      aaaaatggg gaacacagt ctcagcggc cgaaatcgc 
      cgcctccca aatcgtgca ccccaacac tagtctcca 
      ccagaatgc caaaaagca cgagtttca caaaacctt 
      cacacacgg ggaaatacc gccggagtg tcccatgca 
      ccggcccaa gggggcgtc ggagtgtcc cgtcaacca 
      tggcgagat caacccact taacatgat ggggccagc 
      caaccagaa cccatcgcc gggacaacc cttcccgct 
      gcccaccga aagacaagt ctccaacat tccgaaagc 
      catcgtcca ggaggggga ggccccctt ctgggtgta 
      ccccagcca acaaatgtt ttggaacgc aatgttgcg 
      taaagggtg gcgttggaa ggacgggga cgttacgca 
      aaaggacat ggacgatat tatcaaaat ccacaacgc 
      gaataacga gcaggcgtg gcttgaagt cctaaaatg 
      ggaggcgct acatgccaa agagtgtgg taacccaaa 
      attgaaaag ttttggcgg gaaagcaac cacttacag 
      ccctagagc tcgaattcg atggttgct aggttacga 
      attgccatt tgatcgcca tgattggat tgttgacag 
      gtgtggcaa ggaagtgag gtatgtcat agactacta 
      cgatggcgg tatggttga ccagaaata tcagtttgc 
      gttgttgga tgtgaggcc tgctatgga tagctttga 
      gaacatttg ggataggat gactgtggc ttggtggcg 
      atggcggta cggcgatta ataaaccgt gaagaatca 
      cacaaatag tcaagttcg tctcgtgtt ttgtatcta 
      tttatctta attcgtaat aaagcactt tttgcgttt 
      tattgatgt tgtactatt gtctcctag atggcagcc 
      gttcacagc cctcttgac cgcattgtt gcggctttt 
      tttattatg tactgagcg tgtttgggg ctgtcgatg 
      ggagataag cccaagcaa caaatgcgc gtttttgga 
      ctgagtcct gaatttgct tggcggtgt cgtcgcggg 
      gcggtttat cggaagccg ataagtcag tgtgctgat 
      ttgccggcc gtcacgcac acccttcca gcacgcgaa 
      actgacaga cgatttttt ccagcggac caaatgtaa 
      tttaaacct gtaatcgga ccaaaaacc tcttatcag 
      tattggaaa agtattttt atacatttt tataactct 
      aaagtgcat attgtgttg tgttagtac cgtattgtt 
      cgtttcttg tcgctaatt tcctcctct gcttgacgt 
      ttcgccatc ttaacaaac cacttgtta acacatcgt 
      gtttattat ttatttaaa accgtatgc cgtttaacc 
      acgctgtac cagtgctct gaagcctta tttacaacc 
      gcattgtgt ttgtctgcc cttcccgac tccactaac 
      ccccgtttt ccggctgag aatgatcgg gtaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY