General information | |||
TC011718 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 14559447..14561524 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC011718 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01883 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 1 (9 dpi)affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC011718 RA
MASRTTVDF EFGAYTWLG AFRCRISFQ RIGDLFTLL SKVAIIVMK FHVILLFCA YVIGQKAPP LPAADKALV EHYKLALEA VFGVGIRLQ AFLDGGESD NFLVSPISA TVILGQLIL GAEGEFRKQ LVELLALPK GHLYENDVY YIENKKNVT LQQLPYSTL HLQLSNLLK ALESGKDER KHFMLKQKS ALFVDNTVD LNKTFRGNL RNFYQTRIK FFDFQHKQT EALTWINTW VSNNTRGLI TSILPEPPS PSTRSIFAN FIYFKADWE LPFSHELNR EGLFSVTAT ETIPVTYMI NFLEQIPYF ESPDFRLIS IPYVNQELG MYLIQPSSN SPYKYDIKN FVQNLKSRQ ILESVEQAR RKDVVVKIP KMSLRYSFS LLNQLKKYR AFKKTNIKT NSTNVVDKL DDRVDAFNN FTKVDEKDV FLTAAASNG GLRVDEMVQ QVAITINEK GTEAAAVTA ATIDYMGGS KNFVVDRPF VFFIRHEAT AATLFWGTV SKPK
- >TC011718 RA
atggccagc cgaactaca gtcgatttt gaatttggc gcttatact tggcttggt gcatttagg tgtcgcatc agttttcaa cgcatcggc gacctgttc accttgcta tcaaaggtc gcgattata gtgatgaag ttccacgtg attctgttg ttttgcgct tacgtcatt ggccagaag gcgccgccg ctgcccgcc gccgacaag gcgctcgtc gagcactac aaactggcc ctggaggcg gtgttcggc gtcggcatc cgcctccag gcgttcctc gacggcggc gaaagcgac aacttcctc gtcagtccg atcagtgcg actgttatt cttggccag ttgattctt ggagctgaa ggcgagttc cggaagcaa ttagtcgag ttattggcc ttgcccaag ggtcacctc tacgaaaac gatgtctat tacatcgaa aacaagaag aatgtcacg ttgcaacag ttgccctat tccacgctg cacttacag ctgtccaac ctgctgaag gcgctggag agtggcaag gatgaaagg aaacacttt atgctcaag cagaaaagt gcgcttttt gtcgacaac acagtcgac ttgaataaa acgttcaga ggcaatctg aggaacttt tatcagacg aggatcaag ttcttcgac tttcaacac aaacaaaca gaggccttg acctggatc aacacctgg gtgagcaac aacacccgg ggcctcatc acctccatc ctccctgag cccccctcc cccagcact cgctcgatc ttcgccaac ttcatctac ttcaaggcc gactgggaa cttcccttc agccacgag ctgaaccgt gagggcctt ttcagcgtc acggccacc gaaacaatc ccagtaact tacatgatc aacttcctc gagcaaatt ccctacttt gaatccccc gatttccgc ctcatctcc atcccctac gtcaaccaa gagctcggc atgtaccta atccagccg agcagcaac agtccgtac aaatacgac atcaaaaac tttgtccaa aatttgaaa tcgcgccag attttggaa agtgtggaa caagcccgt cgtaaggac gtcgtggtc aaaatcccg aaaatgtcg ctgcggtac agcttcagt ctcctgaac cagttgaag aaatacaga gcgttcaaa aagacaaac attaaaacc aactcgacg aacgtggtc gacaagctc gacgatcga gtcgatgct ttcaacaac ttcacaaaa gtggacgaa aaggacgtt tttctgacc gcggctgcc agtaacggg ggcttgcga gtcgacgaa atggtccaa caagtcgcc atcacgatc aacgaaaag ggcaccgaa gccgccgcc gtcaccgcc gccaccatc gactacatg ggcggctcg aagaacttc gtggtcgac cgccccttc gtcttcttc atccgacac gaggccacc gctgcgacc ctcttctgg ggcaccgtc tccaagccc aaatag
- >TC011718 RA
catctgcga tggccagcc gaactacag tcgattttg aatttggcg cttatactt ggcttggtg catttaggt gtcgcatca gttttcaac gcatcggcg acctgttca ccttgctat caaaggtcg cgattatag tgatgaagt tccacgtga ttctgttgt tttgcgctt acgtcattg gccagaagg cgccgccgc tgcccgccg ccgacaagg cgctcgtcg agcactaca aactggccc tggaggcgg tgttcggcg tcggcatcc gcctccagg cgttcctcg acggcggcg aaagcgaca acttcctcg tcagtccga tcagtgcga ctgttattc ttggccagt tgattcttg gagctgaag gcgagttcc ggaagcaat tagtcgagt tattggcct tgcccaagg gtcacctct acgaaaacg atgtctatt acatcgaaa acaagaaga atgtcacgt tgcaacagt tgccctatt ccacgctgc acttacagc tgtccaacc tgctgaagg cgctggaga gtggcaagg atgaaagga aacacttta tgctcaagc agaaaagtg cgctttttg tcgacaaca cagtcgact tgaataaaa cgttcagag gcaatctga ggaactttt atcagacga ggatcaagt tcttcgact ttcaacaca aacaaacag aggccttga cctggatca acacctggg tgagcaaca acacccggg gcctcatca cctccatcc tccctgagc ccccctccc ccagcactc gctcgatct tcgccaact tcatctact tcaaggccg actgggaac ttcccttca gccacgagc tgaaccgtg agggccttt tcagcgtca cggccaccg aaacaatcc cagtaactt acatgatca acttcctcg agcaaattc cctactttg aatcccccg atttccgcc tcatctcca tcccctacg tcaaccaag agctcggca tgtacctaa tccagccga gcagcaaca gtccgtaca aatacgaca tcaaaaact ttgtccaaa atttgaaat cgcgccaga ttttggaaa gtgtggaac aagcccgtc gtaaggacg tcgtggtca aaatcccga aaatgtcgc tgcggtaca gcttcagtc tcctgaacc agttgaaga aatacagag cgttcaaaa agacaaaca ttaaaacca actcgacga acgtggtcg acaagctcg acgatcgag tcgatgctt tcaacaact tcacaaaag tggacgaaa aggacgttt ttctgaccg cggctgcca gtaacgggg gcttgcgag tcgacgaaa tggtccaac aagtcgcca tcacgatca acgaaaagg gcaccgaag ccgccgccg tcaccgccg ccaccatcg actacatgg gcggctcga agaacttcg tggtcgacc gccccttcg tcttcttca tccgacacg aggccaccg ctgcgaccc tcttctggg gcaccgtct ccaagccca aatagccaa gactgaact agtgatagt gtcattgta tt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
