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Gene information
General information
TC011718 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid67: 6755004..6757081
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011718
    iBeetle screen iB_01883
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 abdomen musculature random partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011718 RA
      MASRTTVDF EFGAYTWLG AFRCRISFQ RIGDLFTLL 
      SKVAIIVMK FHVILLFCA YVIGQKAPP LPAADKALV 
      EHYKLALEA VFGVGIRLQ AFLDGGESD NFLVSPISA 
      TVILGQLIL GAEGEFRKQ LVELLALPK GHLYENDVY 
      YIENKKNVT LQQLPYSTL HLQLSNLLK ALESGKDER 
      KHFMLKQKS ALFVDNTVD LNKTFRGNL RNFYQTRIK 
      FFDFQHKQT EALTWINTW VSNNTRGLI TSILPEPPS 
      PSTRSIFAN FIYFKADWE LPFSHELNR EGLFSVTAT 
      ETIPVTYMI NFLEQIPYF ESPDFRLIS IPYVNQELG 
      MYLIQPSSN SPYKYDIKN FVQNLKSRQ ILESVEQAR 
      RKDVVVKIP KMSLRYSFS LLNQLKKYR AFKKTNIKT 
      NSTNVVDKL DDRVDAFNN FTKVDEKDV FLTAAASNG 
      GLRVDEMVQ QVAITINEK GTEAAAVTA ATIDYMGGS 
      KNFVVDRPF VFFIRHEAT AATLFWGTV SKPK
    CDS sequence
    • >TC011718 RA
      atggccagc cgaactaca gtcgatttt gaatttggc 
      gcttatact tggcttggt gcatttagg tgtcgcatc 
      agttttcaa cgcatcggc gacctgttc accttgcta 
      tcaaaggtc gcgattata gtgatgaag ttccacgtg 
      attctgttg ttttgcgct tacgtcatt ggccagaag 
      gcgccgccg ctgcccgcc gccgacaag gcgctcgtc 
      gagcactac aaactggcc ctggaggcg gtgttcggc 
      gtcggcatc cgcctccag gcgttcctc gacggcggc 
      gaaagcgac aacttcctc gtcagtccg atcagtgcg 
      actgttatt cttggccag ttgattctt ggagctgaa 
      ggcgagttc cggaagcaa ttagtcgag ttattggcc 
      ttgcccaag ggtcacctc tacgaaaac gatgtctat 
      tacatcgaa aacaagaag aatgtcacg ttgcaacag 
      ttgccctat tccacgctg cacttacag ctgtccaac 
      ctgctgaag gcgctggag agtggcaag gatgaaagg 
      aaacacttt atgctcaag cagaaaagt gcgcttttt 
      gtcgacaac acagtcgac ttgaataaa acgttcaga 
      ggcaatctg aggaacttt tatcagacg aggatcaag 
      ttcttcgac tttcaacac aaacaaaca gaggccttg 
      acctggatc aacacctgg gtgagcaac aacacccgg 
      ggcctcatc acctccatc ctccctgag cccccctcc 
      cccagcact cgctcgatc ttcgccaac ttcatctac 
      ttcaaggcc gactgggaa cttcccttc agccacgag 
      ctgaaccgt gagggcctt ttcagcgtc acggccacc 
      gaaacaatc ccagtaact tacatgatc aacttcctc 
      gagcaaatt ccctacttt gaatccccc gatttccgc 
      ctcatctcc atcccctac gtcaaccaa gagctcggc 
      atgtaccta atccagccg agcagcaac agtccgtac 
      aaatacgac atcaaaaac tttgtccaa aatttgaaa 
      tcgcgccag attttggaa agtgtggaa caagcccgt 
      cgtaaggac gtcgtggtc aaaatcccg aaaatgtcg 
      ctgcggtac agcttcagt ctcctgaac cagttgaag 
      aaatacaga gcgttcaaa aagacaaac attaaaacc 
      aactcgacg aacgtggtc gacaagctc gacgatcga 
      gtcgatgct ttcaacaac ttcacaaaa gtggacgaa 
      aaggacgtt tttctgacc gcggctgcc agtaacggg 
      ggcttgcga gtcgacgaa atggtccaa caagtcgcc 
      atcacgatc aacgaaaag ggcaccgaa gccgccgcc 
      gtcaccgcc gccaccatc gactacatg ggcggctcg 
      aagaacttc gtggtcgac cgccccttc gtcttcttc 
      atccgacac gaggccacc gctgcgacc ctcttctgg 
      ggcaccgtc tccaagccc aaatag
    mRNA sequence
    • >TC011718 RA
      catctgcga tggccagcc gaactacag tcgattttg 
      aatttggcg cttatactt ggcttggtg catttaggt 
      gtcgcatca gttttcaac gcatcggcg acctgttca 
      ccttgctat caaaggtcg cgattatag tgatgaagt 
      tccacgtga ttctgttgt tttgcgctt acgtcattg 
      gccagaagg cgccgccgc tgcccgccg ccgacaagg 
      cgctcgtcg agcactaca aactggccc tggaggcgg 
      tgttcggcg tcggcatcc gcctccagg cgttcctcg 
      acggcggcg aaagcgaca acttcctcg tcagtccga 
      tcagtgcga ctgttattc ttggccagt tgattcttg 
      gagctgaag gcgagttcc ggaagcaat tagtcgagt 
      tattggcct tgcccaagg gtcacctct acgaaaacg 
      atgtctatt acatcgaaa acaagaaga atgtcacgt 
      tgcaacagt tgccctatt ccacgctgc acttacagc 
      tgtccaacc tgctgaagg cgctggaga gtggcaagg 
      atgaaagga aacacttta tgctcaagc agaaaagtg 
      cgctttttg tcgacaaca cagtcgact tgaataaaa 
      cgttcagag gcaatctga ggaactttt atcagacga 
      ggatcaagt tcttcgact ttcaacaca aacaaacag 
      aggccttga cctggatca acacctggg tgagcaaca 
      acacccggg gcctcatca cctccatcc tccctgagc 
      ccccctccc ccagcactc gctcgatct tcgccaact 
      tcatctact tcaaggccg actgggaac ttcccttca 
      gccacgagc tgaaccgtg agggccttt tcagcgtca 
      cggccaccg aaacaatcc cagtaactt acatgatca 
      acttcctcg agcaaattc cctactttg aatcccccg 
      atttccgcc tcatctcca tcccctacg tcaaccaag 
      agctcggca tgtacctaa tccagccga gcagcaaca 
      gtccgtaca aatacgaca tcaaaaact ttgtccaaa 
      atttgaaat cgcgccaga ttttggaaa gtgtggaac 
      aagcccgtc gtaaggacg tcgtggtca aaatcccga 
      aaatgtcgc tgcggtaca gcttcagtc tcctgaacc 
      agttgaaga aatacagag cgttcaaaa agacaaaca 
      ttaaaacca actcgacga acgtggtcg acaagctcg 
      acgatcgag tcgatgctt tcaacaact tcacaaaag 
      tggacgaaa aggacgttt ttctgaccg cggctgcca 
      gtaacgggg gcttgcgag tcgacgaaa tggtccaac 
      aagtcgcca tcacgatca acgaaaagg gcaccgaag 
      ccgccgccg tcaccgccg ccaccatcg actacatgg 
      gcggctcga agaacttcg tggtcgacc gccccttcg 
      tcttcttca tccgacacg aggccaccg ctgcgaccc 
      tcttctggg gcaccgtct ccaagccca aatagccaa 
      gactgaact agtgatagt gtcattgta tt