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Gene information
General information
TC011717 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid67: 6767904..6776061
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011717
    iBeetle screen iB_01882
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011717 RA
      MQEIALGYR EVIPDQTVS DPWREITLN TGGTQGTLQ 
      DIKVAERAN GFCYRDSTR HHTRNRFIY WRISHDVLE 
      LVEHSLDIN LAGNRIRYK FVDTPILDG VSIHETYDN 
      VVVLVPTVC SVHRLVFPH PDKYHRQDH VAGVHPDLA 
      APSVFSEAT ANEARDPAT FHFFTNSSS QLPYLAASS 
      LTLAEEEAI FVLAYHSGE ILLLRQSPE GKIDSAELK 
      SESIVPRFL SGLADKLRQ KDKEGDTTV SLILHTFGL 
      ETFALTLCR NGHLKFWSC SKAECVSVI DVMAETGAR 
      NQLQGAQNH ILRKAVDGF DSECLLGIF MSFAAKSQL 
      HVFKPMLSG SQVRLVRLN ILDLAQTDL IDFTISTSR 
      IWSVWRGKE GQCEVYSAS NQAGSHWIP VVLENVPDA 
      SQPLSIDGE TDPRQSYLQ HIFHPGRFP LHIISKALN 
      IYKRSAVIS DLNLSAAHL KQQICMAVE DEVQNELKE 
      TVVSDEDYL ETASYCWQQ FYSCCVQYH VAGLKPLGL 
      LVLPNSSGA VLLKKSMIS FLRPLDIFE HFIYESDHM 
      FRDQFTKYF LLSENVDIT DDVISLFKV INYLDSQMS 
      EIFAYTFER ELALLKSPD VVMGALLEQ IQREMDTEF 
      TEHVAEMVR DCTDLYQSI HKILELLRI EYQIQPEEP 
      IDSVQASIN HIFTSQLGV SMVTGCLRQ QIQVRFTIC 
      RSLLLISNI LLERGELDW NVLEAIRSV CSPEIVVLT 
      QANFVMVWL SGLSALQYL PTESTLQRL SSLRLSPVF 
      NLRTNAISS ISLLELFAG STGGYEARK AFARLRHDE 
      ESLAHWHLA LLPYLNCLF HIIWPISGS PILAEWLLS 
      CGQHLWLQQ YIRYISSWC EWNSCTHSF LLAASLLTT 
      GEPYKAQEL FENAAKGIF TDQFLAQRI LTPDHNQTK 
      AYINYYLKV IHLFELHKS YDCAIALAN KALSVTSPD 
      DPLTATLYS IKFKHHLAL KNYQSAYDS LNANPDSDR 
      RKDNLRDLV KKLLDEKKL DVLMGFTYS GMEELFCDI 
      VSTRARAAD PSNNIYYDF LYAYQIKRG TTFLRAAAS 
      VMYEQAFRL SQYSDPDSL EKQVKCYLA AKNALALCE 
      PQYAYIVMP VEQIEDKTI TLPTFAGSD DEPEEIIVR 
      KQVNIINLE IMSKDLAFA SAKLKLIRF NPRNYFEIT 
      NPHELVILL TSAGLFKTA LTISRTFDV SYRIVFEAL 
      TKKCASLSK QNEPVTWKW LIENNLQDL PVGTKSASE 
      AAWSLLQEC VEEYEEEKM SVIHKIVCK QVIKMNLWI 
      PQWLLSSYK KRNPSELFR LLYTSGRLE EAAEIASEY 
      MMAALGYGK EYYGFAHAM QPTTPAVCL PVRGFITLI 
      NQLKLINSN DPEKPFLRE CEHLSNVLD TYLKTAQRT 
      SIEMCNYHL SLAMN
    CDS sequence
    • >TC011717 RA
      atgcaagaa atcgcgttg gggtaccgg gaggtgatc 
      cccgaccag acggtgtcc gatccctgg cgcgaaatc 
      accctcaac acggggggg acacaaggg acgcttcaa 
      gatatcaaa gtcgcggaa agggccaac gggttttgt 
      taccgcgac tcgaccaga caccacacc aggaatcgg 
      tttatctac tggaggatt agccacgat gtcctggag 
      ctcgtggag cacagtctc gatataaat ctcgccgga 
      aatcgcatc agatacaaa tttgtggac accccgatt 
      ttggacggg gtttccatc catgagacg tacgataat 
      gtggtggtt ctggtgccc acggtgtgc agcgtccat 
      aggttggtg tttccgcat ccggataag taccaccgg 
      caggaccat gtcgcgggg gttcaccca gatctggcg 
      gcgccgtcg gtgttcagc gaagctacg gccaacgag 
      gcgagggat cccgccaca ttccacttt ttcaccaat 
      tcgagttct cagctgcca tatctggcc gcctcttcc 
      ctgaccctc gctgaggag gaagcaatt ttcgtgttg 
      gcctaccac tccggcgag atcctcctt ctgagacag 
      tcccccgag ggcaaaatc gactcagct gagctaaaa 
      agcgagtca atcgtgccc cgctttctc tcaggtctc 
      gccgacaag ctgcgccaa aaagacaaa gaaggcgac 
      actaccgtc agcctcatt ttacatact ttcggtctt 
      gaaactttc gctttgaca ctttgccgc aacggtcat 
      ttgaaattc tggtcgtgc tcaaaggcc gaatgtgtc 
      tcagtgatc gacgtcatg gccgaaacc ggcgccaga 
      aaccagctg caaggcgct cagaaccac atcctgcgg 
      aaagccgtc gatgggttt gattctgag tgtctgttg 
      ggtattttc atgagtttc gcagccaag agtcagttg 
      catgtgttc aagccgatg ctcagtggc tcgcaggtt 
      cggctagtg aggcttaat atacttgat ttggctcag 
      accgatttg atcgatttt acgatctcg acaagtcgg 
      atttggtcg gtttggcgc ggaaaagag ggccaatgt 
      gaggtgtat tcggcctcg aaccaggcg gggagtcac 
      tggattccc gtggttctg gaaaatgtg ccagacgcc 
      agccaaccg ttgagcatc gacggggag acggatccc 
      aggcagagc tacctccag cacatcttc cacccgggc 
      aggttcccc ttgcatata atcagcaag gctttgaac 
      atttacaaa cgctcggcc gtcatcagc gacttgaac 
      ctctcggcg gcccatctc aagcagcag atctgcatg 
      gccgtcgaa gacgaggtc caaaacgag ctgaaggaa 
      accgtcgtc agcgacgaa gattatctc gaaaccgcc 
      agctactgc tggcaacaa ttctactcc tgttgcgtc 
      caataccac gtcgctgga cttaaacct ttaggcctc 
      cttgttctt ccaaactcc tccggggcc gtacttttg 
      aaaaaatcc atgatttcg tttttgcga cctctcgat 
      atttttgaa catttcatc tacgaaagc gaccacatg 
      tttagggac caattcacc aaatatttc ctcttgtct 
      gagaatgtt gatatcaca gacgacgtg atcagtttg 
      ttcaaggtc attaattat ttggattcg cagatgagt 
      gagattttc gcgtacacg ttcgaacga gagttggca 
      ctgctgaag tcgcctgat gtcgtcatg ggggctctc 
      cttgagcag atccagcgc gagatggac accgagttc 
      accgagcac gtggccgag atggtgcgc gattgcacc 
      gatttgtac caatccatc cacaaaatc ctcgaattg 
      ctccgaatc gagtaccaa atccagcca gaggagccc 
      atcgacagc gttcaagcc tcaataaac cacattttc 
      acctcgcag ttgggtgtt tcgatggtt acaggatgt 
      ctccgacag cagatccaa gtccggttc actatctgt 
      cgcagtctt ttgcttatt agtaacatt ttgctcgag 
      cgaggcgaa ctcgattgg aacgtttta gaggcgatt 
      aggtcggtt tgtagtccc gagatcgtg gttttgact 
      caggccaat ttcgtgatg gtttggctg tcgggtttg 
      tccgctttg cagtatttg ccaaccgag tcgacgttg 
      cagagactg tcgtcgttg aggttgagt cctgttttt 
      aatctcagg acgaatgcg attagctcg atttcgttg 
      ttggagctt tttgcgggg agtacaggg gggtatgag 
      gcacggaag gcctttgcg aggttgagg cacgatgag 
      gagagtctg gcgcattgg catttggcg cttttgccg 
      tatcttaat tgcctcttc cacatcatt tggccgatc 
      agtggcagc ccgattttg gccgaatgg ctgctcagt 
      tgtggccaa cacttatgg ctgcagcag tacatccgc 
      tacataagc tcgtggtgc gaatggaat agttgcaca 
      catagcttc cttctcgcc gcgtcttta ctaaccact 
      ggtgaaccg tacaaagcg caagaactg ttcgaaaat 
      gcggccaag ggtattttc acggaccaa tttttggcc 
      caacgcatc ctaacccct gaccacaac caaaccaaa 
      gcctacatt aactactac ttgaaagtg attcatttg 
      ttcgaattg cacaaatcg tacgattgt gcgatcgct 
      ttggccaat aaggcgctc agtgtcacc tcaccggac 
      gaccctttg accgccact ttgtactcc atcaaattc 
      aagcaccat ttggctctg aaaaactac caaagtgcg 
      tatgactcg ctcaatgcc aacccggat tccgacaga 
      cgcaaggac aatttgcgc gacttggtg aaaaaattg 
      ctggatgag aagaagttg gacgttttg atgggtttc 
      acgtacagc gggatggag gagctattt tgtgatatt 
      gtctcgacg agggcgcgc gctgccgac ccctcgaat 
      aatatttac tacgatttt ttgtacgct tatcaaatt 
      aaaagaggg accactttt ttgcgggcc gccgcctct 
      gtgatgtat gaacaagcc ttccgactc agccaatac 
      agtgatcct gactcgttg gaaaaacaa gtcaagtgt 
      taccttgct gcgaaaaac gccctagcc ttgtgtgag 
      cctcaatac gcttacatc gtaatgccc gtggaacaa 
      atcgaggat aaaaccatc actttaccc acttttgcc 
      ggctcggat gacgagcct gaagaaatc atagtacgc 
      aaacaagtg aacatcatc aaccttgaa ataatgtcc 
      aaggacttg gcgttcgcc agcgccaag ctcaagtta 
      attcgcttc aacccgcgc aactacttc gaaataaca 
      aatccgcac gaattggtg attttgttg actagtgcc 
      ggtttgttt aagacggcg ttgacaatt tcgaggact 
      tttgacgtt tcttacagg atagtgttt gaggcactt 
      acgaaaaaa tgcgcgtcg ttgtccaaa cagaacgaa 
      ccggtgact tggaagtgg ctgattgag aacaatttg 
      caagatttg ccagttggg acgaaaagt gcgtcggag 
      gcggcgtgg agcctcctg caggagtgt gtggaggag 
      tacgaggag gagaaaatg tccgttatt cataaaatc 
      gtgtgtaag caggttatt aagatgaat ctgtggata 
      ccgcagtgg ctgctgtcg tcctataag aagcgcaac 
      ccctccgag ctcttccgc ctcttgtac acgtctggc 
      cggctggag gaagccgcc gaaatcgcc tccgagtac 
      atgatggcg gccttgggc tacggtaag gaatactac 
      ggtttcgca cacgccatg caacccaca acaccagcc 
      gtctgtctc cccgttcgc ggtttcata actcttatc 
      aaccaactg aagctgatc aactcaaac gacccggaa 
      aaacccttc ctccgggaa tgcgaacac ttatcaaac 
      gtcctcgat acgtacctc aaaacggca caaagaacc 
      tcgatcgag atgtgcaac taccactta agcctggcg 
      atgaattaa
    mRNA sequence
    • >TC011717 RA
      aaaattatc taaccgcca ctcgccgac cttgatttt 
      ttgtttccg taaacctaa cctaacttt tgcacaaaa 
      aaccgttaa aacgggcta aaaccatgc aagaaatcg 
      cgttggggt accgggagg tgatccccg accagacgg 
      tgtccgatc cctggcgcg aaatcaccc tcaacacgg 
      gggggacac aagggacgc ttcaagata tcaaagtcg 
      cggaaaggg ccaacgggt tttgttacc gcgactcga 
      ccagacacc acaccagga atcggttta tctactgga 
      ggattagcc acgatgtcc tggagctcg tggagcaca 
      gtctcgata taaatctcg ccggaaatc gcatcagat 
      acaaatttg tggacaccc cgattttgg acggggttt 
      ccatccatg agacgtacg ataatgtgg tggttctgg 
      tgcccacgg tgtgcagcg tccataggt tggtgtttc 
      cgcatccgg ataagtacc accggcagg accatgtcg 
      cgggggttc acccagatc tggcggcgc cgtcggtgt 
      tcagcgaag ctacggcca acgaggcga gggatcccg 
      ccacattcc actttttca ccaattcga gttctcagc 
      tgccatatc tggccgcct cttccctga ccctcgctg 
      aggaggaag caattttcg tgttggcct accactccg 
      gcgagatcc tccttctga gacagtccc ccgagggca 
      aaatcgact cagctgagc taaaaagcg agtcaatcg 
      tgccccgct ttctctcag gtctcgccg acaagctgc 
      gccaaaaag acaaagaag gcgacacta ccgtcagcc 
      tcattttac atactttcg gtcttgaaa ctttcgctt 
      tgacacttt gccgcaacg gtcatttga aattctggt 
      cgtgctcaa aggccgaat gtgtctcag tgatcgacg 
      tcatggccg aaaccggcg ccagaaacc agctgcaag 
      gcgctcaga accacatcc tgcggaaag ccgtcgatg 
      ggtttgatt ctgagtgtc tgttgggta ttttcatga 
      gtttcgcag ccaagagtc agttgcatg tgttcaagc 
      cgatgctca gtggctcgc aggttcggc tagtgaggc 
      ttaatatac ttgatttgg ctcagaccg atttgatcg 
      attttacga tctcgacaa gtcggattt ggtcggttt 
      ggcgcggaa aagagggcc aatgtgagg tgtattcgg 
      cctcgaacc aggcgggga gtcactgga ttcccgtgg 
      ttctggaaa atgtgccag acgccagcc aaccgttga 
      gcatcgacg gggagacgg atcccaggc agagctacc 
      tccagcaca tcttccacc cgggcaggt tccccttgc 
      atataatca gcaaggctt tgaacattt acaaacgct 
      cggccgtca tcagcgact tgaacctct cggcggccc 
      atctcaagc agcagatct gcatggccg tcgaagacg 
      aggtccaaa acgagctga aggaaaccg tcgtcagcg 
      acgaagatt atctcgaaa ccgccagct actgctggc 
      aacaattct actcctgtt gcgtccaat accacgtcg 
      ctggactta aacctttag gcctccttg ttcttccaa 
      actcctccg gggccgtac ttttgaaaa aatccatga 
      tttcgtttt tgcgacctc tcgatattt ttgaacatt 
      tcatctacg aaagcgacc acatgttta gggaccaat 
      tcaccaaat atttcctct tgtctgaga atgttgata 
      tcacagacg acgtgatca gtttgttca aggtcatta 
      attatttgg attcgcaga tgagtgaga ttttcgcgt 
      acacgttcg aacgagagt tggcactgc tgaagtcgc 
      ctgatgtcg tcatggggg ctctccttg agcagatcc 
      agcgcgaga tggacaccg agttcaccg agcacgtgg 
      ccgagatgg tgcgcgatt gcaccgatt tgtaccaat 
      ccatccaca aaatcctcg aattgctcc gaatcgagt 
      accaaatcc agccagagg agcccatcg acagcgttc 
      aagcctcaa taaaccaca ttttcacct cgcagttgg 
      gtgtttcga tggttacag gatgtctcc gacagcaga 
      tccaagtcc ggttcacta tctgtcgca gtcttttgc 
      ttattagta acattttgc tcgagcgag gcgaactcg 
      attggaacg ttttagagg cgattaggt cggtttgta 
      gtcccgaga tcgtggttt tgactcagg ccaatttcg 
      tgatggttt ggctgtcgg gtttgtccg ctttgcagt 
      atttgccaa ccgagtcga cgttgcaga gactgtcgt 
      cgttgaggt tgagtcctg tttttaatc tcaggacga 
      atgcgatta gctcgattt cgttgttgg agctttttg 
      cggggagta caggggggt atgaggcac ggaaggcct 
      ttgcgaggt tgaggcacg atgaggaga gtctggcgc 
      attggcatt tggcgcttt tgccgtatc ttaattgcc 
      tcttccaca tcatttggc cgatcagtg gcagcccga 
      ttttggccg aatggctgc tcagttgtg gccaacact 
      tatggctgc agcagtaca tccgctaca taagctcgt 
      ggtgcgaat ggaatagtt gcacacata gcttccttc 
      tcgccgcgt ctttactaa ccactggtg aaccgtaca 
      aagcgcaag aactgttcg aaaatgcgg ccaagggta 
      ttttcacgg accaatttt tggcccaac gcatcctaa 
      cccctgacc acaaccaaa ccaaagcct acattaact 
      actacttga aagtgattc atttgttcg aattgcaca 
      aatcgtacg attgtgcga tcgctttgg ccaataagg 
      cgctcagtg tcacctcac cggacgacc ctttgaccg 
      ccactttgt actccatca aattcaagc accatttgg 
      ctctgaaaa actaccaaa gtgcgtatg actcgctca 
      atgccaacc cggattccg acagacgca aggacaatt 
      tgcgcgact tggtgaaaa aattgctgg atgagaaga 
      agttggacg ttttgatgg gtttcacgt acagcggga 
      tggaggagc tattttgtg atattgtct cgacgaggg 
      cgcgcgctg ccgacccct cgaataata tttactacg 
      attttttgt acgcttatc aaattaaaa gagggacca 
      cttttttgc gggccgccg cctctgtga tgtatgaac 
      aagccttcc gactcagcc aatacagtg atcctgact 
      cgttggaaa aacaagtca agtgttacc ttgctgcga 
      aaaacgccc tagccttgt gtgagcctc aatacgctt 
      acatcgtaa tgcccgtgg aacaaatcg aggataaaa 
      ccatcactt tacccactt ttgccggct cggatgacg 
      agcctgaag aaatcatag tacgcaaac aagtgaaca 
      tcatcaacc ttgaaataa tgtccaagg acttggcgt 
      tcgccagcg ccaagctca agttaattc gcttcaacc 
      cgcgcaact acttcgaaa taacaaatc cgcacgaat 
      tggtgattt tgttgacta gtgccggtt tgtttaaga 
      cggcgttga caatttcga ggacttttg acgtttctt 
      acaggatag tgtttgagg cacttacga aaaaatgcg 
      cgtcgttgt ccaaacaga acgaaccgg tgacttgga 
      agtggctga ttgagaaca atttgcaag atttgccag 
      ttgggacga aaagtgcgt cggaggcgg cgtggagcc 
      tcctgcagg agtgtgtgg aggagtacg aggaggaga 
      aaatgtccg ttattcata aaatcgtgt gtaagcagg 
      ttattaaga tgaatctgt ggataccgc agtggctgc 
      tgtcgtcct ataagaagc gcaacccct ccgagctct 
      tccgcctct tgtacacgt ctggccggc tggaggaag 
      ccgccgaaa tcgcctccg agtacatga tggcggcct 
      tgggctacg gtaaggaat actacggtt tcgcacacg 
      ccatgcaac ccacaacac cagccgtct gtctccccg 
      ttcgcggtt tcataactc ttatcaacc aactgaagc 
      tgatcaact caaacgacc cggaaaaac ccttcctcc 
      gggaatgcg aacacttat caaacgtcc tcgatacgt 
      acctcaaaa cggcacaaa gaacctcga tcgagatgt 
      gcaactacc acttaagcc tggcgatga attaataaa 
      gtgattgtt tcgtaaaaa agtgtttta tttcgtttt 
      cattcgtac gtttcggtt acgctcccc actgtggtg 
      aaggtcagg tagatggtg acgatgagg aggcagccg 
      gcgactgtg gtgataacc agggttggg tgctgtcgg 
      aagactatc agcaggagc acgctggcg accagcatg 
      atagccgcc aggagcgga atcaggatc agaggggcc 
      cattcgttg cctctgatt tcttgaaaa aaaaattgt 
      tattgctat aacaaaact cgccaaaaa ctgcgtgat 
      ctattatta tttcgaaga attcgccga aaaacctta 
      tctgcagtt tacacttgc ggttaaaga gctccttga 
      tctgttatt tcgagtaga acaagtcgc actttagcg 
      tttatggca aaaaatgct cacctgcgc ccaaggagg 
      tgccaaacc cgtccttaa aatcccacg tccttttgc 
      tcacattca tccgtttcg aatgcgaat aataaatct 
      tcggtcatt gttgcgata accggtgat aagcaaagg 
      tcgctcgcc ggttttccc gatcaggtt ttccccact 
      atagccaac cgcacaaca caaaatcga aaaatctct 
      ccgtgtcgc acacagaat a