| General information | |||
| TC011717 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 14572347..14580504 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC011717 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01882 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC011717 RA
MQEIALGYR EVIPDQTVS DPWREITLN TGGTQGTLQ DIKVAERAN GFCYRDSTR HHTRNRFIY WRISHDVLE LVEHSLDIN LAGNRIRYK FVDTPILDG VSIHETYDN VVVLVPTVC SVHRLVFPH PDKYHRQDH VAGVHPDLA APSVFSEAT ANEARDPAT FHFFTNSSS QLPYLAASS LTLAEEEAI FVLAYHSGE ILLLRQSPE GKIDSAELK SESIVPRFL SGLADKLRQ KDKEGDTTV SLILHTFGL ETFALTLCR NGHLKFWSC SKAECVSVI DVMAETGAR NQLQGAQNH ILRKAVDGF DSECLLGIF MSFAAKSQL HVFKPMLSG SQVRLVRLN ILDLAQTDL IDFTISTSR IWSVWRGKE GQCEVYSAS NQAGSHWIP VVLENVPDA SQPLSIDGE TDPRQSYLQ HIFHPGRFP LHIISKALN IYKRSAVIS DLNLSAAHL KQQICMAVE DEVQNELKE TVVSDEDYL ETASYCWQQ FYSCCVQYH VAGLKPLGL LVLPNSSGA VLLKKSMIS FLRPLDIFE HFIYESDHM FRDQFTKYF LLSENVDIT DDVISLFKV INYLDSQMS EIFAYTFER ELALLKSPD VVMGALLEQ IQREMDTEF TEHVAEMVR DCTDLYQSI HKILELLRI EYQIQPEEP IDSVQASIN HIFTSQLGV SMVTGCLRQ QIQVRFTIC RSLLLISNI LLERGELDW NVLEAIRSV CSPEIVVLT QANFVMVWL SGLSALQYL PTESTLQRL SSLRLSPVF NLRTNAISS ISLLELFAG STGGYEARK AFARLRHDE ESLAHWHLA LLPYLNCLF HIIWPISGS PILAEWLLS CGQHLWLQQ YIRYISSWC EWNSCTHSF LLAASLLTT GEPYKAQEL FENAAKGIF TDQFLAQRI LTPDHNQTK AYINYYLKV IHLFELHKS YDCAIALAN KALSVTSPD DPLTATLYS IKFKHHLAL KNYQSAYDS LNANPDSDR RKDNLRDLV KKLLDEKKL DVLMGFTYS GMEELFCDI VSTRARAAD PSNNIYYDF LYAYQIKRG TTFLRAAAS VMYEQAFRL SQYSDPDSL EKQVKCYLA AKNALALCE PQYAYIVMP VEQIEDKTI TLPTFAGSD DEPEEIIVR KQVNIINLE IMSKDLAFA SAKLKLIRF NPRNYFEIT NPHELVILL TSAGLFKTA LTISRTFDV SYRIVFEAL TKKCASLSK QNEPVTWKW LIENNLQDL PVGTKSASE AAWSLLQEC VEEYEEEKM SVIHKIVCK QVIKMNLWI PQWLLSSYK KRNPSELFR LLYTSGRLE EAAEIASEY MMAALGYGK EYYGFAHAM QPTTPAVCL PVRGFITLI NQLKLINSN DPEKPFLRE CEHLSNVLD TYLKTAQRT SIEMCNYHL SLAMN
- >TC011717 RA
atgcaagaa atcgcgttg gggtaccgg gaggtgatc cccgaccag acggtgtcc gatccctgg cgcgaaatc accctcaac acggggggg acacaaggg acgcttcaa gatatcaaa gtcgcggaa agggccaac gggttttgt taccgcgac tcgaccaga caccacacc aggaatcgg tttatctac tggaggatt agccacgat gtcctggag ctcgtggag cacagtctc gatataaat ctcgccgga aatcgcatc agatacaaa tttgtggac accccgatt ttggacggg gtttccatc catgagacg tacgataat gtggtggtt ctggtgccc acggtgtgc agcgtccat aggttggtg tttccgcat ccggataag taccaccgg caggaccat gtcgcgggg gttcaccca gatctggcg gcgccgtcg gtgttcagc gaagctacg gccaacgag gcgagggat cccgccaca ttccacttt ttcaccaat tcgagttct cagctgcca tatctggcc gcctcttcc ctgaccctc gctgaggag gaagcaatt ttcgtgttg gcctaccac tccggcgag atcctcctt ctgagacag tcccccgag ggcaaaatc gactcagct gagctaaaa agcgagtca atcgtgccc cgctttctc tcaggtctc gccgacaag ctgcgccaa aaagacaaa gaaggcgac actaccgtc agcctcatt ttacatact ttcggtctt gaaactttc gctttgaca ctttgccgc aacggtcat ttgaaattc tggtcgtgc tcaaaggcc gaatgtgtc tcagtgatc gacgtcatg gccgaaacc ggcgccaga aaccagctg caaggcgct cagaaccac atcctgcgg aaagccgtc gatgggttt gattctgag tgtctgttg ggtattttc atgagtttc gcagccaag agtcagttg catgtgttc aagccgatg ctcagtggc tcgcaggtt cggctagtg aggcttaat atacttgat ttggctcag accgatttg atcgatttt acgatctcg acaagtcgg atttggtcg gtttggcgc ggaaaagag ggccaatgt gaggtgtat tcggcctcg aaccaggcg gggagtcac tggattccc gtggttctg gaaaatgtg ccagacgcc agccaaccg ttgagcatc gacggggag acggatccc aggcagagc tacctccag cacatcttc cacccgggc aggttcccc ttgcatata atcagcaag gctttgaac atttacaaa cgctcggcc gtcatcagc gacttgaac ctctcggcg gcccatctc aagcagcag atctgcatg gccgtcgaa gacgaggtc caaaacgag ctgaaggaa accgtcgtc agcgacgaa gattatctc gaaaccgcc agctactgc tggcaacaa ttctactcc tgttgcgtc caataccac gtcgctgga cttaaacct ttaggcctc cttgttctt ccaaactcc tccggggcc gtacttttg aaaaaatcc atgatttcg tttttgcga cctctcgat atttttgaa catttcatc tacgaaagc gaccacatg tttagggac caattcacc aaatatttc ctcttgtct gagaatgtt gatatcaca gacgacgtg atcagtttg ttcaaggtc attaattat ttggattcg cagatgagt gagattttc gcgtacacg ttcgaacga gagttggca ctgctgaag tcgcctgat gtcgtcatg ggggctctc cttgagcag atccagcgc gagatggac accgagttc accgagcac gtggccgag atggtgcgc gattgcacc gatttgtac caatccatc cacaaaatc ctcgaattg ctccgaatc gagtaccaa atccagcca gaggagccc atcgacagc gttcaagcc tcaataaac cacattttc acctcgcag ttgggtgtt tcgatggtt acaggatgt ctccgacag cagatccaa gtccggttc actatctgt cgcagtctt ttgcttatt agtaacatt ttgctcgag cgaggcgaa ctcgattgg aacgtttta gaggcgatt aggtcggtt tgtagtccc gagatcgtg gttttgact caggccaat ttcgtgatg gtttggctg tcgggtttg tccgctttg cagtatttg ccaaccgag tcgacgttg cagagactg tcgtcgttg aggttgagt cctgttttt aatctcagg acgaatgcg attagctcg atttcgttg ttggagctt tttgcgggg agtacaggg gggtatgag gcacggaag gcctttgcg aggttgagg cacgatgag gagagtctg gcgcattgg catttggcg cttttgccg tatcttaat tgcctcttc cacatcatt tggccgatc agtggcagc ccgattttg gccgaatgg ctgctcagt tgtggccaa cacttatgg ctgcagcag tacatccgc tacataagc tcgtggtgc gaatggaat agttgcaca catagcttc cttctcgcc gcgtcttta ctaaccact ggtgaaccg tacaaagcg caagaactg ttcgaaaat gcggccaag ggtattttc acggaccaa tttttggcc caacgcatc ctaacccct gaccacaac caaaccaaa gcctacatt aactactac ttgaaagtg attcatttg ttcgaattg cacaaatcg tacgattgt gcgatcgct ttggccaat aaggcgctc agtgtcacc tcaccggac gaccctttg accgccact ttgtactcc atcaaattc aagcaccat ttggctctg aaaaactac caaagtgcg tatgactcg ctcaatgcc aacccggat tccgacaga cgcaaggac aatttgcgc gacttggtg aaaaaattg ctggatgag aagaagttg gacgttttg atgggtttc acgtacagc gggatggag gagctattt tgtgatatt gtctcgacg agggcgcgc gctgccgac ccctcgaat aatatttac tacgatttt ttgtacgct tatcaaatt aaaagaggg accactttt ttgcgggcc gccgcctct gtgatgtat gaacaagcc ttccgactc agccaatac agtgatcct gactcgttg gaaaaacaa gtcaagtgt taccttgct gcgaaaaac gccctagcc ttgtgtgag cctcaatac gcttacatc gtaatgccc gtggaacaa atcgaggat aaaaccatc actttaccc acttttgcc ggctcggat gacgagcct gaagaaatc atagtacgc aaacaagtg aacatcatc aaccttgaa ataatgtcc aaggacttg gcgttcgcc agcgccaag ctcaagtta attcgcttc aacccgcgc aactacttc gaaataaca aatccgcac gaattggtg attttgttg actagtgcc ggtttgttt aagacggcg ttgacaatt tcgaggact tttgacgtt tcttacagg atagtgttt gaggcactt acgaaaaaa tgcgcgtcg ttgtccaaa cagaacgaa ccggtgact tggaagtgg ctgattgag aacaatttg caagatttg ccagttggg acgaaaagt gcgtcggag gcggcgtgg agcctcctg caggagtgt gtggaggag tacgaggag gagaaaatg tccgttatt cataaaatc gtgtgtaag caggttatt aagatgaat ctgtggata ccgcagtgg ctgctgtcg tcctataag aagcgcaac ccctccgag ctcttccgc ctcttgtac acgtctggc cggctggag gaagccgcc gaaatcgcc tccgagtac atgatggcg gccttgggc tacggtaag gaatactac ggtttcgca cacgccatg caacccaca acaccagcc gtctgtctc cccgttcgc ggtttcata actcttatc aaccaactg aagctgatc aactcaaac gacccggaa aaacccttc ctccgggaa tgcgaacac ttatcaaac gtcctcgat acgtacctc aaaacggca caaagaacc tcgatcgag atgtgcaac taccactta agcctggcg atgaattaa
- >TC011717 RA
aaaattatc taaccgcca ctcgccgac cttgatttt ttgtttccg taaacctaa cctaacttt tgcacaaaa aaccgttaa aacgggcta aaaccatgc aagaaatcg cgttggggt accgggagg tgatccccg accagacgg tgtccgatc cctggcgcg aaatcaccc tcaacacgg gggggacac aagggacgc ttcaagata tcaaagtcg cggaaaggg ccaacgggt tttgttacc gcgactcga ccagacacc acaccagga atcggttta tctactgga ggattagcc acgatgtcc tggagctcg tggagcaca gtctcgata taaatctcg ccggaaatc gcatcagat acaaatttg tggacaccc cgattttgg acggggttt ccatccatg agacgtacg ataatgtgg tggttctgg tgcccacgg tgtgcagcg tccataggt tggtgtttc cgcatccgg ataagtacc accggcagg accatgtcg cgggggttc acccagatc tggcggcgc cgtcggtgt tcagcgaag ctacggcca acgaggcga gggatcccg ccacattcc actttttca ccaattcga gttctcagc tgccatatc tggccgcct cttccctga ccctcgctg aggaggaag caattttcg tgttggcct accactccg gcgagatcc tccttctga gacagtccc ccgagggca aaatcgact cagctgagc taaaaagcg agtcaatcg tgccccgct ttctctcag gtctcgccg acaagctgc gccaaaaag acaaagaag gcgacacta ccgtcagcc tcattttac atactttcg gtcttgaaa ctttcgctt tgacacttt gccgcaacg gtcatttga aattctggt cgtgctcaa aggccgaat gtgtctcag tgatcgacg tcatggccg aaaccggcg ccagaaacc agctgcaag gcgctcaga accacatcc tgcggaaag ccgtcgatg ggtttgatt ctgagtgtc tgttgggta ttttcatga gtttcgcag ccaagagtc agttgcatg tgttcaagc cgatgctca gtggctcgc aggttcggc tagtgaggc ttaatatac ttgatttgg ctcagaccg atttgatcg attttacga tctcgacaa gtcggattt ggtcggttt ggcgcggaa aagagggcc aatgtgagg tgtattcgg cctcgaacc aggcgggga gtcactgga ttcccgtgg ttctggaaa atgtgccag acgccagcc aaccgttga gcatcgacg gggagacgg atcccaggc agagctacc tccagcaca tcttccacc cgggcaggt tccccttgc atataatca gcaaggctt tgaacattt acaaacgct cggccgtca tcagcgact tgaacctct cggcggccc atctcaagc agcagatct gcatggccg tcgaagacg aggtccaaa acgagctga aggaaaccg tcgtcagcg acgaagatt atctcgaaa ccgccagct actgctggc aacaattct actcctgtt gcgtccaat accacgtcg ctggactta aacctttag gcctccttg ttcttccaa actcctccg gggccgtac ttttgaaaa aatccatga tttcgtttt tgcgacctc tcgatattt ttgaacatt tcatctacg aaagcgacc acatgttta gggaccaat tcaccaaat atttcctct tgtctgaga atgttgata tcacagacg acgtgatca gtttgttca aggtcatta attatttgg attcgcaga tgagtgaga ttttcgcgt acacgttcg aacgagagt tggcactgc tgaagtcgc ctgatgtcg tcatggggg ctctccttg agcagatcc agcgcgaga tggacaccg agttcaccg agcacgtgg ccgagatgg tgcgcgatt gcaccgatt tgtaccaat ccatccaca aaatcctcg aattgctcc gaatcgagt accaaatcc agccagagg agcccatcg acagcgttc aagcctcaa taaaccaca ttttcacct cgcagttgg gtgtttcga tggttacag gatgtctcc gacagcaga tccaagtcc ggttcacta tctgtcgca gtcttttgc ttattagta acattttgc tcgagcgag gcgaactcg attggaacg ttttagagg cgattaggt cggtttgta gtcccgaga tcgtggttt tgactcagg ccaatttcg tgatggttt ggctgtcgg gtttgtccg ctttgcagt atttgccaa ccgagtcga cgttgcaga gactgtcgt cgttgaggt tgagtcctg tttttaatc tcaggacga atgcgatta gctcgattt cgttgttgg agctttttg cggggagta caggggggt atgaggcac ggaaggcct ttgcgaggt tgaggcacg atgaggaga gtctggcgc attggcatt tggcgcttt tgccgtatc ttaattgcc tcttccaca tcatttggc cgatcagtg gcagcccga ttttggccg aatggctgc tcagttgtg gccaacact tatggctgc agcagtaca tccgctaca taagctcgt ggtgcgaat ggaatagtt gcacacata gcttccttc tcgccgcgt ctttactaa ccactggtg aaccgtaca aagcgcaag aactgttcg aaaatgcgg ccaagggta ttttcacgg accaatttt tggcccaac gcatcctaa cccctgacc acaaccaaa ccaaagcct acattaact actacttga aagtgattc atttgttcg aattgcaca aatcgtacg attgtgcga tcgctttgg ccaataagg cgctcagtg tcacctcac cggacgacc ctttgaccg ccactttgt actccatca aattcaagc accatttgg ctctgaaaa actaccaaa gtgcgtatg actcgctca atgccaacc cggattccg acagacgca aggacaatt tgcgcgact tggtgaaaa aattgctgg atgagaaga agttggacg ttttgatgg gtttcacgt acagcggga tggaggagc tattttgtg atattgtct cgacgaggg cgcgcgctg ccgacccct cgaataata tttactacg attttttgt acgcttatc aaattaaaa gagggacca cttttttgc gggccgccg cctctgtga tgtatgaac aagccttcc gactcagcc aatacagtg atcctgact cgttggaaa aacaagtca agtgttacc ttgctgcga aaaacgccc tagccttgt gtgagcctc aatacgctt acatcgtaa tgcccgtgg aacaaatcg aggataaaa ccatcactt tacccactt ttgccggct cggatgacg agcctgaag aaatcatag tacgcaaac aagtgaaca tcatcaacc ttgaaataa tgtccaagg acttggcgt tcgccagcg ccaagctca agttaattc gcttcaacc cgcgcaact acttcgaaa taacaaatc cgcacgaat tggtgattt tgttgacta gtgccggtt tgtttaaga cggcgttga caatttcga ggacttttg acgtttctt acaggatag tgtttgagg cacttacga aaaaatgcg cgtcgttgt ccaaacaga acgaaccgg tgacttgga agtggctga ttgagaaca atttgcaag atttgccag ttgggacga aaagtgcgt cggaggcgg cgtggagcc tcctgcagg agtgtgtgg aggagtacg aggaggaga aaatgtccg ttattcata aaatcgtgt gtaagcagg ttattaaga tgaatctgt ggataccgc agtggctgc tgtcgtcct ataagaagc gcaacccct ccgagctct tccgcctct tgtacacgt ctggccggc tggaggaag ccgccgaaa tcgcctccg agtacatga tggcggcct tgggctacg gtaaggaat actacggtt tcgcacacg ccatgcaac ccacaacac cagccgtct gtctccccg ttcgcggtt tcataactc ttatcaacc aactgaagc tgatcaact caaacgacc cggaaaaac ccttcctcc gggaatgcg aacacttat caaacgtcc tcgatacgt acctcaaaa cggcacaaa gaacctcga tcgagatgt gcaactacc acttaagcc tggcgatga attaataaa gtgattgtt tcgtaaaaa agtgtttta tttcgtttt cattcgtac gtttcggtt acgctcccc actgtggtg aaggtcagg tagatggtg acgatgagg aggcagccg gcgactgtg gtgataacc agggttggg tgctgtcgg aagactatc agcaggagc acgctggcg accagcatg atagccgcc aggagcgga atcaggatc agaggggcc cattcgttg cctctgatt tcttgaaaa aaaaattgt tattgctat aacaaaact cgccaaaaa ctgcgtgat ctattatta tttcgaaga attcgccga aaaacctta tctgcagtt tacacttgc ggttaaaga gctccttga tctgttatt tcgagtaga acaagtcgc actttagcg tttatggca aaaaatgct cacctgcgc ccaaggagg tgccaaacc cgtccttaa aatcccacg tccttttgc tcacattca tccgtttcg aatgcgaat aataaatct tcggtcatt gttgcgata accggtgat aagcaaagg tcgctcgcc ggttttccc gatcaggtt ttccccact atagccaac cgcacaaca caaaatcga aaaatctct ccgtgtcgc acacagaat a
