Status
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Gene information
General information
TC011712 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14603977..14608034
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011712
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01881

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011712 RA
      MSNVVCFTL RGGRSVLCK GTVDLSSGK VCKILACKF 
      STKPRRSKI FTDIAETVK DIPNGSKLL VGGFGLCGI 
      PENLIAALL AHPSQNYTV VSNNAGVEN FGLGLLLQK 
      RKIKRMISS YVGENPEFE RQYLSGELE LELTPQGTL 
      AERIRAGGA GIPAFYTPT AFGTLVHEG GAPICYDKE 
      GKIKIASPP RQAQTFGGK NYIMEEAIT GDFALIKAW 
      KADPYGNLI FNKSARNFN PAMAKAAKI TIAEVEEIV 
      EVGQIGPDE VHLPGIFVD RVVKGTKYE KRIEKVCVS 
      EEGSGKGGG KSPAALLRE RIIRRAALE FKDGMYANL 
      GIGMPMLAS NYIPNDMEV FLQSENGIL GLGPFPTSK 
      NVDPDLINA GKETVTVVP GASYFGSDD SFAMIRGGH 
      IDLTMLGAM EVSQFGDLA NWMIPKKLV KGMGGAMDL 
      VAAPGTKVV VCMEHNAKN GSPKILEKC TLPLTGKQC 
      VDMIITEKA VFEIDQTKG LTLVEIADE VTEEDVKKA 
      TACKFEVSK DLKKMGQI
    CDS sequence
    • >TC011712 RA
      atgtcgaac gtggtttgt tttactttg agaggtgga 
      aggagtgtg ttatgtaag ggaacggtg gatttgtcg 
      tccggaaag gtgtgcaag atcttggct tgtaaattc 
      tccaccaag ccccgccgc agcaaaatc ttcaccgac 
      atcgccgaa accgtcaag gacatcccc aatggctcc 
      aaactcctc gttggaggt ttcggcctt tgcggcatc 
      cccgaaaac ctcatcgcc gcccttttg gcccacccc 
      tcccagaac tacaccgtc gtctccaac aacgccggt 
      gtcgaaaac ttcggcttg ggcctcctt ctccagaaa 
      cgcaaaatc aaacgaatg atttcctcc tacgttgga 
      gaaaatccc gaatttgaa cgccaatac ctgtccgga 
      gaactggaa ctcgagctc acgccacag ggaactctc 
      gccgaacgg attcgggct ggaggtgcc gggatcccg 
      gctttttac acacctact gccttcggg acgttggtc 
      catgaaggc ggagccccg atttgctac gacaaggag 
      gggaaaatc aaaattgcg agccctcca agacaagca 
      cagaccttt ggagggaaa aactacatc atggaggag 
      gccatcacg ggggatttc gccctcatc aaggcctgg 
      aaagccgac ccttacggc aacttgatt tttaacaaa 
      tcggcccgg aattttaac ccggccatg gccaaagcg 
      gccaaaatc accatcgcc gaagtggag gaaatagtc 
      gaggtgggc cagataggg cctgatgag gtccacttg 
      cctggaatt ttcgtcgac agagtcgtc aaaggcaca 
      aaatacgag aaaagaatc gagaaagtg tgtgttagt 
      gaggaaggg agtggtaaa gggggtggc aaatcacca 
      gctgccctc ctacgggag cggatcatc cgtagggcc 
      gctctggag tttaaggac gggatgtat gccaatttg 
      gggatcggg atgccgatg ctcgcctcc aactacatc 
      cccaacgac atggaggtg ttcctccaa tccgagaac 
      gggattctg ggattaggg ccgttccca acctccaaa 
      aacgtggac ccggacctg atcaatgcc gggaaggag 
      actgtgact gtggtcccg ggagcgtcg tacttcggg 
      agtgatgat agttttgcc atgattcgg ggagggcac 
      attgatttg acaatgttg ggggcaatg gaggtgtcg 
      caatttggc gacttggcc aactggatg atcccgaaa 
      aagcttgtc aaaggaatg ggtggtgcg atggacctg 
      gtggcggcc cccggaacc aaagtggtg gtctgtatg 
      gagcacaac gccaagaac ggaagtccc aaaattttg 
      gaaaagtgc accttgccc ctaactggc aaacaatgc 
      gttgacatg atcatcacc gagaaggct gtgtttgaa 
      atcgaccaa accaaaggt ctcaccctt gttgaaatc 
      gccgacgaa gtcacggaa gaagacgtc aagaaggcc 
      acagcttgt aaattcgaa gtgtcgaaa gatttgaaa 
      aaaatgggt caaatttaa
    mRNA sequence
    • >TC011712 RA
      tttcaatac tacggcagc tgtggcgag ttacttgcg 
      ttttcgcac aatgtcgaa cgtggtttg ttttacttt 
      gagaggtgg aaggagtgt gttatgtaa gggaacggt 
      ggatttgtc gtccggaaa ggtgtgcaa gatcttggc 
      ttgtaaatt ctccaccaa gccccgccg cagcaaaat 
      cttcaccga catcgccga aaccgtcaa ggacatccc 
      caatggctc caaactcct cgttggagg tttcggcct 
      ttgcggcat ccccgaaaa cctcatcgc cgccctttt 
      ggcccaccc ctcccagaa ctacaccgt cgtctccaa 
      caacgccgg tgtcgaaaa cttcggctt gggcctcct 
      tctccagaa acgcaaaat caaacgaat gatttcctc 
      ctacgttgg agaaaatcc cgaatttga acgccaata 
      cctgtccgg agaactgga actcgagct cacgccaca 
      gggaactct cgccgaacg gattcgggc tggaggtgc 
      cgggatccc ggcttttta cacacctac tgccttcgg 
      gacgttggt ccatgaagg cggagcccc gatttgcta 
      cgacaagga ggggaaaat caaaattgc gagccctcc 
      aagacaagc acagacctt tggagggaa aaactacat 
      catggagga ggccatcac gggggattt cgccctcat 
      caaggcctg gaaagccga cccttacgg caacttgat 
      ttttaacaa atcggcccg gaattttaa cccggccat 
      ggccaaagc ggccaaaat caccatcgc cgaagtgga 
      ggaaatagt cgaggtggg ccagatagg gcctgatga 
      ggtccactt gcctggaat tttcgtcga cagagtcgt 
      caaaggcac aaaatacga gaaaagaat cgagaaagt 
      gtgtgttag tgaggaagg gagtggtaa agggggtgg 
      caaatcacc agctgccct cctacggga gcggatcat 
      ccgtagggc cgctctgga gtttaagga cgggatgta 
      tgccaattt ggggatcgg gatgccgat gctcgcctc 
      caactacat ccccaacga catggaggt gttcctcca 
      atccgagaa cgggattct gggattagg gccgttccc 
      aacctccaa aaacgtgga cccggacct gatcaatgc 
      cgggaagga gactgtgac tgtggtccc gggagcgtc 
      gtacttcgg gagtgatga tagttttgc catgattcg 
      gggagggca cattgattt gacaatgtt gggggcaat 
      ggaggtgtc gcaatttgg cgacttggc caactggat 
      gatcccgaa aaagcttgt caaaggaat gggtggtgc 
      gatggacct ggtggcggc ccccggaac caaagtggt 
      ggtctgtat ggagcacaa cgccaagaa cggaagtcc 
      caaaatttt ggaaaagtg caccttgcc cctaactgg 
      caaacaatg cgttgacat gatcatcac cgagaaggc 
      tgtgtttga aatcgacca aaccaaagg tctcaccct 
      tgttgaaat cgccgacga agtcacgga agaagacgt 
      caagaaggc cacagcttg taaattcga agtgtcgaa 
      agatttgaa aaaaatggg tcaaattta ataaaattt 
      tgaaaaaaa tcttgtgtt ttattcgcg aaacttggc 
      cttgacgaa ggccggaag cgggattag gtacaaaaa 
      caagttgaa gacgaccat tccaagagc gaaggcgaa 
      agtgatccc aatgttcat gtcaaagtc gaggattcc 
      atcgctgaa aataacgtc agtcttgtc ccttgagta 
      cctctccac tagaaattc ctgtccgtt tgagtaccg 
      acaaaagcc ggagctgct cgaagccgc cgtttcgat 
      caaattcat gttggtaca attgtgctt caggtcgta 
      cggtagtgt ttcaagtag ttccggaat taaaaacgt 
      ctgtcgatt gaggaaagc ggacgcgta ccgcacttg 
      agtagcata ggttaggta gtacaacca ttgttgtaa 
      ccctttgat cga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY