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Gene information
General information
TC011710 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid67: 6810866..6813221
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011710
    iBeetle screen iB_01880
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen partially dorsoventral-axis broader - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011710 RA
      MLRRSLVIL RQFSTSGPK SGPAGRFQY 
      TIRAAFGKY LLVTNTVSS GVLMLLGDI 
      VEQEFHHDF KAREDEPRY DYGRLGRMF 
      LVGLGMGPV HHYYYGLIN KLWPLRDMV 
      TVSKKILAD QIVMSPICI AQFFYTLGL 
      LEQKPVKRI SEEFLGKFG AVYTMDWCV 
      WPPTQFINF YLIPCRYQV IYINFVTML 
      YNVFLSYIK HEY
    CDS sequence
    • >TC011710 RA
      atgttgcgt cggagtttg gtcattttg cgccaattt 
      tcaacttct gggccaaaa tcggggcct gcgggccgc 
      ttccagtac acgattagg gcagctttc ggcaagtac 
      ctgctagtg acaaacact gtgagctcg ggggtgctc 
      atgcttctg ggggacatc gtggagcaa gagttccac 
      cacgatttc aaggcccgc gaggacgag ccccgttat 
      gattacggc cgtttaggc agaatgttt ctggtgggg 
      ttggggatg ggacccgtc caccactac tattacggg 
      ttgatcaac aaattgtgg cctttgcgc gacatggtg 
      acggttagc aaaaaaatt ctagccgac caaattgtc 
      atgtccccg atttgtata gcccagttt ttctacaca 
      ctagggcta ttagaacag aaacctgta aagcggata 
      agtgaggag tttttaggc aaatttggg gctgtttat 
      acgatggat tggtgtgtg tggccaccg acgcagttt 
      atcaatttt tatttaatc ccctgtaga taccaagtt 
      atttatatt aatttcgtc acaatgctt tacaacgta 
      tttttgtcg tatattaag cacgagtat tga
    mRNA sequence
    • >TC011710 RA
      agtggaaag tggtgattg ttggcgtat tgtcaccac 
      tggacattg acgagacaa taaataatt tcacttatt 
      tacattagt cgggcgaaa gggtaaatc gcactagcg 
      atgacccca gcctgccgg gtgctacgc ctctggtga 
      ttaaattaa ttaaaaata ccttttcga gcggatttt 
      gtacggctt caggagaac tcggcattt tatttgatt 
      atatctcat tccggccca gtttccgcc cactttcat 
      ccgattttc agccaagac ccggaaatc aataagctg 
      aaaactcgc accaaccac tggaatttg tctacactc 
      aatttcact caaacaaaa atttcaaac aacaaaaaa 
      ccacatcag aaaataaca caacaaaaa acccgtgac 
      gccatttat aaacaaact ttgacagtt tttttactc 
      aaagccccg ccccgccac ctagtagcc agtagatgt 
      ggcgattat aacgtcatt gaaacgtca cttgcaaaa 
      aaaaattcg caaaaatgt acttaataa ttacaacat 
      attcacatt atattactc aattgtcac tcttttgtg 
      taaataaag cttaatgga ttacgcata ggtgttttc 
      gctcgcaaa tttttccaa gtgtgacag ttacataac 
      ctgcgatgt tgcgtcgga gtttggtca ttttgcgcc 
      aattttcaa cttctgggc caaaatcgg ggcctgcgg 
      gccgcttcc agtacacga ttagggcag ctttcggca 
      agtacctgc tagtgacaa acactgtga gctcggggg 
      tgctcatgc ttctggggg acatcgtgg agcaagagt 
      tccaccacg atttcaagg cccgcgagg acgagcccc 
      gttatgatt acggccgtt taggcagaa tgtttctgg 
      tggggttgg ggatgggac ccgtccacc actactatt 
      acgggttga tcaacaaat tgtggcctt tgcgcgaca 
      tggtgacgg ttagcaaaa aaattctag ccgaccaaa 
      ttgtcatgt ccccgattt gtatagccc agtttttct 
      acacactag ggctattag aacagaaac ctgtaaagc 
      ggataagtg aggagtttt taggcaaat ttggggctg 
      tttatacga tggattggt gtgtgtggc caccgacgc 
      agtttatca atttttatt taatcccct gtagatacc 
      aagttattt atattaatt tcgtcacaa tgctttaca 
      acgtatttt tgtcgtata ttaagcacg agtattgac 
      caatctaat taggaccaa acgtactta ctgccaaat 
      aaagaatga taaattat