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Gene information
General information
TC011666 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 16123917..16127706
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011666
    iBeetle screen iB_01874

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 16.67% (includes death as larva, prepupa, pupa, adult)

    Adult morphology
    wings not closed - Number of animals showing the phenotype: 2


    pronotum bristle dorsal mediansurface not present - Number of animals showing the phenotype: 7

    Comment: dorsal medium line without bristles

    abdominal odoriferous glands colour decreased - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Comment: ovaries were extremely held-egg (>10 eggs in one lateral oviduct)

    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011666 RA
      MFVPPPVEQ QFRMDPNMT LLHSSSQIH NVHPRRKNN 
      RNTLHGMQA ERLSLSEEA KLEMASLPT KTPVSVLQE 
      LLSRRGATP KYELVQIEG AIHEPIFRY RVFINNDLV 
      ATGTGRSKK DAKHAAAKN LLDVLVGKQ SPEQANASN 
      GTPGANDIT AQVVSPFDD KVMGNPIGW LQEMCMSRR 
      WPPPSYEME HEEGLPHER QFTIACQVL KFKEVGTGK 
      SKKLAKRMA AHKMWQALQ DMPLEGNNL PQGYDDDEE 
      LAAKMCNLQ GRYSGLKDS KIPTLNIQH TQKVSQFHK 
      ALKQSNGPK LKELQNIVL NSKDFNFIQ FLHEIASEQ 
      QFEVTYVDI EEKALSGKS QCLVQLSTL PVAVCYGAG 
      ATPKEAQSA AALNALEYL RIMSKK
    CDS sequence
    • >TC011666 RA
      atgtttgta cctccgcca gttgaacag caatttcgc 
      atggaccca aatatgacg cttctgcat tcctcaagt 
      caaatacac aacgtccat cctaggagg aaaaacaac 
      agaaatact ttacacgga atgcaggct gaacgcttg 
      tctctgagc gaagaggct aaattggag atggcgtcg 
      ttgccgacc aaaactcct gtttcagta cttcaggag 
      ttattaagt cgtcgtggg gcgacgcct aagtacgaa 
      ctagtccaa atcgagggg gctattcac gaacctata 
      ttccgctac agggtgttt attaataac gatttggtt 
      gctactgga acagggagg tcgaaaaaa gacgccaaa 
      cacgctgct gcgaagaat ctgcttgat gttttggtc 
      ggcaaacag agtccagaa caagccaac gcttctaac 
      gggactccc ggagccaac gatattaca gctcaagtg 
      gtgtctccg tttgatgat aaagttatg gggaaccct 
      atcggttgg ctccaggag atgtgcatg tctcggaga 
      tggcctcca ccgtcctat gagatggaa catgaggaa 
      ggtcttcca cacgaacgc cagttcacg atagcttgt 
      caagtgtta aaattcaaa gaagtcggt actggcaag 
      tcaaaaaaa ttagctaaa agaatggca gcacacaaa 
      atgtggcag gccctgcaa gatatgcct ctagagggc 
      aataatctg ccgcaaggt tacgacgac gatgaagag 
      ttggcggcg aaaatgtgt aacttacaa ggacgctac 
      agtggtctg aaagatagt aaaataccg acactcaac 
      atacaacac acccaaaag gtgtcacag tttcacaaa 
      gccctcaag cagtccaat gggccgaag ctaaaggag 
      ttgcagaac atagttctc aacagcaaa gacttcaac 
      ttcattcag tttttgcat gaaattgcg tccgagcaa 
      caatttgaa gtaacttat gtcgatata gaggagaaa 
      gcactatct ggcaaaagt cagtgctta gtacagctt 
      tctacatta ccggtagcg gtgtgttac ggtgccgga 
      gcgacaccg aaggaagcg caatcagcg gctgctttg 
      aatgcactc gaatattta agaataatg agcaagaag 
      tga
    mRNA sequence
    • >TC011666 RA
      catgcgaac tgtttaacc atttgacgt ttgtgtcca 
      tcaaaaaac gtcgccatt acagtgttt atagcgacg 
      attgtattt caaagtgta ataaaaaat tctaaataa 
      ttggcgaat ggaatataa tttgctcgt tttcttcag 
      tacgggaag cggaaatta agggccgac aagcggcga 
      gatgtttgt acctccgcc agttgaaca gcaatttcg 
      catggaccc aaatatgac gcttctgca ttcctcaag 
      tcaaataca caacgtcca tcctaggag gaaaaacaa 
      cagaaatac tttacacgg aatgcaggc tgaacgctt 
      gtctctgag cgaagaggc taaattgga gatggcgtc 
      gttgccgac caaaactcc tgtttcagt acttcagga 
      gttattaag tcgtcgtgg ggcgacgcc taagtacga 
      actagtcca aatcgaggg ggctattca cgaacctat 
      attccgcta cagggtgtt tattaataa cgatttggt 
      tgctactgg aacagggag gtcgaaaaa agacgccaa 
      acacgctgc tgcgaagaa tctgcttga tgttttggt 
      cggcaaaca gagtccaga acaagccaa cgcttctaa 
      cgggactcc cggagccaa cgatattac agctcaagt 
      ggtgtctcc gtttgatga taaagttat ggggaaccc 
      tatcggttg gctccagga gatgtgcat gtctcggag 
      atggcctcc accgtccta tgagatgga acatgagga 
      aggtcttcc acacgaacg ccagttcac gatagcttg 
      tcaagtgtt aaaattcaa agaagtcgg tactggcaa 
      gtcaaaaaa attagctaa aagaatggc agcacacaa 
      aatgtggca ggccctgca agatatgcc tctagaggg 
      caataatct gccgcaagg ttacgacga cgatgaaga 
      gttggcggc gaaaatgtg taacttaca aggacgcta 
      cagtggtct gaaagatag taaaatacc gacactcaa 
      catacaaca cacccaaaa ggtgtcaca gtttcacaa 
      agccctcaa gcagtccaa tgggccgaa gctaaagga 
      gttgcagaa catagttct caacagcaa agacttcaa 
      cttcattca gtttttgca tgaaattgc gtccgagca 
      acaatttga agtaactta tgtcgatat agaggagaa 
      agcactatc tggcaaaag tcagtgctt agtacagct 
      ttctacatt accggtagc ggtgtgtta cggtgccgg 
      agcgacacc gaaggaagc gcaatcagc ggctgcttt 
      gaatgcact cgaatattt aagaataat gagcaagaa 
      gtgaatttc gagcttatg atcgtttac acttcaaac 
      atctgcaac ttttttatt taatttgtt tttattgtg 
      tatacttat ttaggctaa tccatgttg agatgtttt 
      taagtgtag ccgagtcat tttttcaaa caaaaatgt 
      atcaagatg catgtgctt tccctacat gcattttgc 
      ttttcttct cgatcggcg ccattttgg taacacgtg 
      atggatgaa tcttgggcg gcgtcgaca attttacga 
      ttacataca tcacagtca gttgggcac tttttattg 
      tgtattgtt caaatgcga aacaataaa aactgtgcg 
      acttattaa cgagaagga acgcaccta attttacaa 
      gctatactt tgcatagaa ttagtggtt tttttcgag 
      actatgtat gtcgttttt tatattttt gtgtttgtg 
      tgtgtgtta atgcactca agcagaaaa tgatttttt 
      aaaaggtgt ctattatta agagtagtg atgtttagg 
      tacctagct ttagcattc cactatata ggcactaga 
      acttgagtt gtaatgttg ggtgccccc atataacat 
      gttgtacat atttatatt gtgtaaagg gagcattta 
      tttaatgca aagtataga ggttgtacc cctgtttcc 
      ccagaatta acatatatg tcaatagaa gctaatgtt 
      aagatcaag tacatcaat tcggacaat attgttttt 
      cctttatta tgttctact tgagccata tcgatattg 
      atgtgtatg ctaattgaa aataaaata atagcttac 
      aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY