Status
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Gene information
General information
TC011654 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 16048967..16054995
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011654
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01873

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011654 RA
      MMTPPAADD DLVSAGEWL ESNAAVVEQ WLKRHASTE 
      FRRRVQEAV ADARRVSVA PDLFQRWLV HPPAVRPRR 
      DLEAMEEDE LFMELIRDV ANELDIDVL CHKILRNVS 
      LLTRADRGS LFLARGDGD GKYLVAKLF DVRHDTSLE 
      EAVKMAKAE EIRIPFGVG IAGTVAKNK ILINITDAY 
      NDPRFSSDI DLKTGYKTN SILCMPICN YEGEVIGVA 
      QIINKTNDS QEFTSHDVE VFRRYLTFC GIGIQNAQL 
      FQVSVLEYK RNQILLNLA RSIFEEQNN LECLVTKIM 
      REAQDLLKC ERCAVYLMD LDCGEANHL ERILERPGR 
      IIRRHAPLN RVESGSVTA EDMRVSGLK TPCSFTTIF 
      ELRDGGSNT LVSRPSSFD LQCSTLAQI ASYAASTGQ 
      ILNIGDVKT WLSEQHSEG DAESARTIL CMPILNGQR 
      NLIGVAQLI NKENGAPFT ECDVSLFEA FAIFCGLGI 
      HNTQMYENA CKLMAKQKV ALECLSYHA TASNDATEK 
      LIKEDIPSA ETYNLYSFT FIDFELSDD DTCKATIRM 
      FLECNLVQQ FHIPYEVLC RWILSVKKN YRPVKYHNW 
      RHALNVAQT MFAMFKTGK MERFMSDLE MLGLLVACL 
      CHDLDHRGT NNAFQTKTE SPLAILYST STMEHHHFD 
      QCVMILNTD SNNIFQALS TEDYRRVMK LVETAILST 
      DLAMYFKKR NKFLELIEN GEFDWQSDD KKDLLSGMM 
      MTACDVSAI AKPWEVQHR VAKLVADEF FDQGDMEKM 
      QLKEQPIAM MDRERKDEL PQMQVGFID VICLPLYRV 
      LSETFPWVK PLYQGTLEN RKHWQDLAE KVEMGLTWI 
      DHDTIDKPV EAFADTEET KDIELTVQT LHTAPACQQ 
      LPPLGHKLK RNKHKLCCI L
    CDS sequence
    • >TC011654 RA
      atgatgacg ccacctgcc gccgatgat gacctggtg 
      tcggcgggc gaatggctg gaatcgaac gccgccgtc 
      gtcgagcag tggcttaaa cggcacgcc tccacagag 
      tttcgtaga cgcgtccag gaagccgtt gcagatgcc 
      agacgcgtc tccgtcgct ccagatctt ttccagcga 
      tggctggtg catccacct gcagtgcgg cccagacgc 
      gacctggag gctatggag gaggacgaa ttgtttatg 
      gagttgata cgggacgtc gccaacgag ctagacatt 
      gacgtgctg tgccacaag atattgagg aatgtgagc 
      ttgttgaca agagccgac agggggtcc ttgtttctc 
      gctagggga gatggagac ggcaagtat ctggtggcg 
      aagctgttt gatgttaga catgatacc tcactggag 
      gaggccgtc aaaatggca aaggccgaa gaaattagg 
      attcccttt ggggtgggc attgcgggg accgtcgcc 
      aaaaataaa atactcatc aacatcact gatgcctac 
      aatgatccg cgtttcagc agcgacata gaccttaaa 
      acgggctac aagaccaac tcgatacta tgcatgcct 
      atttgcaat tacgaaggc gaagttatt ggagtcgcg 
      caaattatt aacaagacc aacgatagt caggagttt 
      acttcgcac gacgtcgag gttttcagg aggtatttg 
      actttctgt gggattgga atacaaaac gcccagcta 
      ttccaagtc tcggtgctg gagtacaag aggaatcag 
      attttgcta aacctggca cgtagcatt ttcgaagaa 
      cagaataat ttagaatgt ctggtgacg aaaataatg 
      agagaagcg caagattta ttaaagtgc gagagatgc 
      gccgtgtac ttaatggat ctggattgt ggagaagcg 
      aatcatttg gaacggatt ttagaacgt ccggggaga 
      ataattcga cgtcatgct cctctaaac cgtgtcgaa 
      agtggatcg gtgacggct gaagacatg agagtgagt 
      ggcttaaaa actccttgt tctttcaca acaattttc 
      gaattgaga gatggaggg agtaatact ttggtatct 
      agaccttct tccttcgat ttgcaatgt tccactttg 
      gcacaaatt gcaagttat gccgcttcc accggtcaa 
      attctcaat atcggagat gttaaaacg tggcttagt 
      gaacaacac agtgaaggc gatgccgaa tcggccaga 
      accatcctc tgtatgcct atactcaac ggccaaaga 
      aatctcata ggtgttgcc cagctaata aataaggaa 
      aacggcgcc ccgtttacc gaatgtgac gtttctttg 
      ttcgaagct tttgcgatt ttttgcggt ttgggtatt 
      cataatacc cagatgtac gaaaacgct tgcaagttg 
      atggcgaag cagaaagtc gctttagaa tgtctttct 
      tatcacgcg acggcaagt aacgacgct acggaaaaa 
      ctgatcaaa gaggatatc ccttcagct gaaacttac 
      aacttgtat agcttcacg ttcatcgat ttcgaactt 
      tcagacgac gatacttgc aaagccaca atacggatg 
      tttttagaa tgcaacttg gtgcaacaa tttcacatt 
      ccctacgag gtcttgtgt cgttggatc ctgagcgta 
      aaaaagaat tatcgtccg gtgaaatac cacaattgg 
      agacatgct ctaaacgtg gcccagaca atgtttgcc 
      atgttcaaa acgggaaaa atggagcga tttatgagc 
      gatttggaa atgttgggg cttttggtc gcctgtttg 
      tgtcatgat ttagaccat cgaggtaca aataacgct 
      ttccaaacc aaaaccgag tctccactt gcgattttg 
      tactctacc agtactatg gagcaccat cactttgac 
      cagtgtgtc atgattctc aataccgac agcaataat 
      atattccaa gctttatca acggaggac taccggagg 
      gtgatgaag ctcgtagaa actgcgatt ttatcgact 
      gatctcgcc atgtacttc aaaaagagg aacaaattt 
      ctggaactg attgaaaac ggcgaattc gattggcaa 
      agcgacgat aaaaaggac ctactttcc ggaatgatg 
      atgactgct tgcgatgtg agtgcgatc gcaaaaccc 
      tgggaagtg caacatcga gtggcgaaa ctagtcgct 
      gatgagttt ttcgaccag ggagatatg gaaaaaatg 
      caactgaaa gaacaacca atcgcgatg atggatcgt 
      gaaagaaaa gacgaattg cctcagatg caagtggga 
      tttatcgac gtcatttgt ttaccgctt taccgagtt 
      ttatcggaa acgtttcct tgggttaaa cccttgtat 
      caagggact ttggagaat cgcaagcat tggcaggat 
      ttggccgaa aaagtcgaa atgggcctt acgtggatt 
      gatcacgat accattgat aaaccggtt gaagcgttc 
      gctgataca gaagagacc aaagatata gaattaaca 
      gttcaaact ctacacacc gctccagca tgtcagcaa 
      ttgcctcct ttgggacac aaattgaaa cgtaataaa 
      cacaagtta tgttgcata ttgtga
    mRNA sequence
    • >TC011654 RA
      cagtcggca tgagccagt gcagttctc agtcctcca 
      acacctgga cgccatcct cgcggtgtc tctaaggac 
      atgcgggac gatgatgac gccacctgc cgccgatga 
      tgacctggt gtcggcggg cgaatggct ggaatcgaa 
      cgccgccgt cgtcgagca gtggcttaa acggcacgc 
      ctccacaga gtttcgtag acgcgtcca ggaagccgt 
      tgcagatgc cagacgcgt ctccgtcgc tccagatct 
      tttccagcg atggctggt gcatccacc tgcagtgcg 
      gcccagacg cgacctgga ggctatgga ggaggacga 
      attgtttat ggagttgat acgggacgt cgccaacga 
      gctagacat tgacgtgct gtgccacaa gatattgag 
      gaatgtgag cttgttgac aagagccga cagggggtc 
      cttgtttct cgctagggg agatggaga cggcaagta 
      tctggtggc gaagctgtt tgatgttag acatgatac 
      ctcactgga ggaggccgt caaaatggc aaaggccga 
      agaaattag gattccctt tggggtggg cattgcggg 
      gaccgtcgc caaaaataa aatactcat caacatcac 
      tgatgccta caatgatcc gcgtttcag cagcgacat 
      agaccttaa aacgggcta caagaccaa ctcgatact 
      atgcatgcc tatttgcaa ttacgaagg cgaagttat 
      tggagtcgc gcaaattat taacaagac caacgatag 
      tcaggagtt tacttcgca cgacgtcga ggttttcag 
      gaggtattt gactttctg tgggattgg aatacaaaa 
      cgcccagct attccaagt ctcggtgct ggagtacaa 
      gaggaatca gattttgct aaacctggc acgtagcat 
      tttcgaaga acagaataa tttagaatg tctggtgac 
      gaaaataat gagagaagc gcaagattt attaaagtg 
      cgagagatg cgccgtgta cttaatgga tctggattg 
      tggagaagc gaatcattt ggaacggat tttagaacg 
      tccggggag aataattcg acgtcatgc tcctctaaa 
      ccgtgtcga aagtggatc ggtgacggc tgaagacat 
      gagagtgag tggcttaaa aactccttg ttctttcac 
      aacaatttt cgaattgag agatggagg gagtaatac 
      tttggtatc tagaccttc ttccttcga tttgcaatg 
      ttccacttt ggcacaaat tgcaagtta tgccgcttc 
      caccggtca aattctcaa tatcggaga tgttaaaac 
      gtggcttag tgaacaaca cagtgaagg cgatgccga 
      atcggccag aaccatcct ctgtatgcc tatactcaa 
      cggccaaag aaatctcat aggtgttgc ccagctaat 
      aaataagga aaacggcgc cccgtttac cgaatgtga 
      cgtttcttt gttcgaagc ttttgcgat tttttgcgg 
      tttgggtat tcataatac ccagatgta cgaaaacgc 
      ttgcaagtt gatggcgaa gcagaaagt cgctttaga 
      atgtctttc ttatcacgc gacggcaag taacgacgc 
      tacggaaaa actgatcaa agaggatat cccttcagc 
      tgaaactta caacttgta tagcttcac gttcatcga 
      tttcgaact ttcagacga cgatacttg caaagccac 
      aatacggat gtttttaga atgcaactt ggtgcaaca 
      atttcacat tccctacga ggtcttgtg tcgttggat 
      cctgagcgt aaaaaagaa ttatcgtcc ggtgaaata 
      ccacaattg gagacatgc tctaaacgt ggcccagac 
      aatgtttgc catgttcaa aacgggaaa aatggagcg 
      atttatgag cgatttgga aatgttggg gcttttggt 
      cgcctgttt gtgtcatga tttagacca tcgaggtac 
      aaataacgc tttccaaac caaaaccga gtctccact 
      tgcgatttt gtactctac cagtactat ggagcacca 
      tcactttga ccagtgtgt catgattct caataccga 
      cagcaataa tatattcca agctttatc aacggagga 
      ctaccggag ggtgatgaa gctcgtaga aactgcgat 
      tttatcgac tgatctcgc catgtactt caaaaagag 
      gaacaaatt tctggaact gattgaaaa cggcgaatt 
      cgattggca aagcgacga taaaaagga cctactttc 
      cggaatgat gatgactgc ttgcgatgt gagtgcgat 
      cgcaaaacc ctgggaagt gcaacatcg agtggcgaa 
      actagtcgc tgatgagtt tttcgacca gggagatat 
      ggaaaaaat gcaactgaa agaacaacc aatcgcgat 
      gatggatcg tgaaagaaa agacgaatt gcctcagat 
      gcaagtggg atttatcga cgtcatttg tttaccgct 
      ttaccgagt tttatcgga aacgtttcc ttgggttaa 
      acccttgta tcaagggac tttggagaa tcgcaagca 
      ttggcagga tttggccga aaaagtcga aatgggcct 
      tacgtggat tgatcacga taccattga taaaccggt 
      tgaagcgtt cgctgatac agaagagac caaagatat 
      agaattaac agttcaaac tctacacac cgctccagc 
      atgtcagca attgcctcc tttgggaca caaattgaa 
      acgtaataa acacaagtt atgttgcat attgtgaca 
      tacccccca taaagtgtc attaattag tgttttctt 
      acacattgt cattgatat tctagagaa aaaaaaatg 
      ctcataata tgttacatc agggatggt ttttttaca 
      aacaacaaa aaagaaaag agcagaatg gtgatgctt 
      gaatccaaa aaagacatt ttgtaatta tatttgtat 
      ctaacagca aataaactg tatatagaa aataaaaag 
      taaatttgg aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY