| General information | |||
| TC011645 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 15947553..15951091 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC011645 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01872 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC011645 RA
MDNDLERSV RLLEDALQT PTKGLFKVS TATPKREKP NLSFTINPE DLTGLEYGS VYQNTLSLF VDDDKTVTG SVIQAGTPW KNTVNNLYT EFADILQGS TGGLDILDV AEDLARCCS DALAVVKSL KAKVATSEL AEEKWLENE RNNWRLLYV LYQDRIQAQ NHSKEGFEE DFGSSEKNC VCELFKREN LVRESQLVI DWLERNALE KGDPILMHS DWTYGWEHT LHQLLAPDA IAIGSNVDI IKNIHPDAP QFQRLSLHH LDYDNDKKL CQRIFMEIR CGNLEGAQE LCYNSGYPW RAAMLEGWK LFHNPNLET SLEEYKDVE GNQRRDLWK SCALDYCHR SNLNIYEKA AIGSLCGFL EGILPVCTT WEDHLWAYM RTMVDIRVE SEIRDSIIK NYIPLPEEY WEQKKSLSE IFTILEASK NIDIAKEAH EIDHVIQKL VILDEIPTL IRLLDQWSE DLSVTTQSL RFYAHLVLF FESVGDSTH RKTCEKIVE TYIERLSEM NETQLVAFY VSKLGKSKQ IQLYAKHCE KLLSTEQRK NALTYAEDF SLDVFAITQ QIVEIITSR PSESELPGN LQGKITEID ELKISAIDW LMFYEEQRA EALAQTNSI IFKFLILGK LEAAQLAIN KIPYDSIEK IRSEEQIHQ IVKNYLSYK AYLDAHEAF NEWFRQYRN KPMPPPELP ENAQFTEKV AHNHKVSQF NAEAERWKL TTSHSAKRA KTLLYNVLL FPEGWLEGG DDAEYLRSI CIPQIVLLL YSVLNESGL HEEAVQLAD LVAAKKYGL YKVYSKEKL AEILEKICE SSVALLNAK KDPFGSSSS V
- >TC011645 RA
atggataat gatttggaa cggtcggtg cgtcttctc gaagacgct ctccagacc cccacaaaa gggctattt aaggtctcg accgcaact ccaaagcgt gaaaaacct aacctcagc tttacaata aaccccgaa gacttaacc ggcctggaa tatggaagt gtttatcaa aacaccttg tctttattt gtcgatgat gacaaaacg gtcaccggg tcggtgatc caagcagga actccatgg aaaaacact gttaataat ttatataca gagtttgcg gatatcctg caggggtcc accggaggc cttgatatc ctggacgtg gccgaagac ttggcgagg tgttgtagt gacgctttg gcggtggtt aagtcactg aaagccaaa gtggccact agtgaatta gccgaagag aaatggctc gaaaatgag cggaataat tggagactg ctgtatgtc ctgtaccag gatcggatc caggcccag aaccacagt aaggaaggg tttgaggag gatttcggg agcagtgaa aagaactgt gtgtgcgaa ctgttcaaa cgagagaat ttggttagg gaaagccag ttggtgatc gattggctc gagaggaac gctctggag aagggggac cccatttta atgcactca gactggacg tatggatgg gaacacact ttgcatcaa ttgctagca cctgatgct attgcaatt gggagtaat gtggatatt atcaagaac atacatcca gatgcgcca cagtttcag cgcttatcg ctgcaccac ttggattac gacaatgac aaaaaacta tgtcagaga atttttatg gaaattagg tgcggcaac cttgagggc gcccaggaa ttatgttac aacagtggt tatccatgg cgcgctgcg atgttagaa ggctggaag ctttttcat aatcccaac ctggaaaca agtcttgaa gagtataaa gacgtagaa gggaaccaa aggcgggat ttgtggaaa tcttgcgca ttagattat tgtcatagg tcaaacttg aacatttat gaaaaagcc gccattggt agtctttgt ggtttcttg gagggcatt ttgcccgtt tgtaccaca tgggaggac cacctgtgg gcctacatg cgaaccatg gtcgatatt cgtgttgaa tcggaaatt cgcgactca atcattaaa aactacata ccactacct gaagaatat tgggaacaa aagaaatca ttaagtgaa atttttaca attttggaa gcctcgaaa aatattgac attgcaaaa gaagcgcat gaaattgat catgtaatt cagaaactt gtcatttta gacgaaatt cccacactg atacgcctc ctagatcag tggagtgaa gatctaagt gttacgaca caatctctt cgattttat gctcactta gttttgttt tttgaaagc gtcggagac tccactcat agaaaaacg tgtgaaaaa atcgtcgaa acatatatc gagcgtttg agcgaaatg aacgaaacc cagcttgtg gccttctac gtgagcaag ctgggaaaa tccaaacaa atccaactt tacgccaaa cactgcgaa aaactgctt tcgactgag caacgcaaa aacgccctt acttacgct gaagatttc agtttggac gtatttgct ataacgcaa caaatcgtc gaaattata acaagccga ccgtctgaa tccgaactt cccggtaat ctccaaggt aaaatcacc gaaatcgac gaactcaaa atctccgct attgattgg ctgatgttt tacgaggag caacgagcc gaagcattg gctcaaacc aattctatt attttcaaa tttctgatt ttgggcaaa ttagaagca gcccaattg gcaatcaat aaaattcct tacgattct atagagaag atacgcagc gaagagcag attcatcag attgttaag aattatttg agttataaa gcgtatttg gatgcacat gaggcgttt aacgagtgg tttagacag tataggaat aagccgatg ccaccacct gaactgccc gaaaatgca caatttact gaaaaagtt gctcataat cataaagtc tcgcagttt aatgccgaa gcggaacgg tggaaattg acgacatcg cattcagca aaaagggcc aaaacttta ttatataat gtccttttg tttcctgaa gggtggctt gaaggagga gacgatgct gagtatctg cgatcaatt tgtattcct cagattgtt ttactattg tattcggtt ttaaatgag tctggactg cacgaagaa gcggtgcag ttggccgat ttagtggcg gccaagaaa tacggactt tacaaggtg tattctaaa gagaaattg gcagagatt ttggagaag atttgtgaa agttctgtg gcgcttctc aacgcgaag aaagacccg tttggaagc tcctcaagt gtttaa
- >TC011645 RA
tcaacactt ttttcacca attttcctt caatttgaa caaaaaaca cgataaaca caaacagac gcgataatt tgaaacaaa tgacgatcg aggaaaaga aaaactgtt accaacctg aatctaggg actttgcaa cgctctgag gcaacgcta gacttgttt tttgaggtt atgcaacgg ctttttgtg tgtgttttt tattaaaaa tggataatg atttggaac ggtcggtgc gtcttctcg aagacgctc tccagaccc ccacaaaag ggctattta aggtctcga ccgcaactc caaagcgtg aaaaaccta acctcagct ttacaataa accccgaag acttaaccg gcctggaat atggaagtg tttatcaaa acaccttgt ctttatttg tcgatgatg acaaaacgg tcaccgggt cggtgatcc aagcaggaa ctccatgga aaaacactg ttaataatt tatatacag agtttgcgg atatcctgc aggggtcca ccggaggcc ttgatatcc tggacgtgg ccgaagact tggcgaggt gttgtagtg acgctttgg cggtggtta agtcactga aagccaaag tggccacta gtgaattag ccgaagaga aatggctcg aaaatgagc ggaataatt ggagactgc tgtatgtcc tgtaccagg atcggatcc aggcccaga accacagta aggaagggt ttgaggagg atttcggga gcagtgaaa agaactgtg tgtgcgaac tgttcaaac gagagaatt tggttaggg aaagccagt tggtgatcg attggctcg agaggaacg ctctggaga agggggacc ccattttaa tgcactcag actggacgt atggatggg aacacactt tgcatcaat tgctagcac ctgatgcta ttgcaattg ggagtaatg tggatatta tcaagaaca tacatccag atgcgccac agtttcagc gcttatcgc tgcaccact tggattacg acaatgaca aaaaactat gtcagagaa tttttatgg aaattaggt gcggcaacc ttgagggcg cccaggaat tatgttaca acagtggtt atccatggc gcgctgcga tgttagaag gctggaagc tttttcata atcccaacc tggaaacaa gtcttgaag agtataaag acgtagaag ggaaccaaa ggcgggatt tgtggaaat cttgcgcat tagattatt gtcataggt caaacttga acatttatg aaaaagccg ccattggta gtctttgtg gtttcttgg agggcattt tgcccgttt gtaccacat gggaggacc acctgtggg cctacatgc gaaccatgg tcgatattc gtgttgaat cggaaattc gcgactcaa tcattaaaa actacatac cactacctg aagaatatt gggaacaaa agaaatcat taagtgaaa tttttacaa ttttggaag cctcgaaaa atattgaca ttgcaaaag aagcgcatg aaattgatc atgtaattc agaaacttg tcattttag acgaaattc ccacactga tacgcctcc tagatcagt ggagtgaag atctaagtg ttacgacac aatctcttc gattttatg ctcacttag ttttgtttt ttgaaagcg tcggagact ccactcata gaaaaacgt gtgaaaaaa tcgtcgaaa catatatcg agcgtttga gcgaaatga acgaaaccc agcttgtgg ccttctacg tgagcaagc tgggaaaat ccaaacaaa tccaacttt acgccaaac actgcgaaa aactgcttt cgactgagc aacgcaaaa acgccctta cttacgctg aagatttca gtttggacg tatttgcta taacgcaac aaatcgtcg aaattataa caagccgac cgtctgaat ccgaacttc ccggtaatc tccaaggta aaatcaccg aaatcgacg aactcaaaa tctccgcta ttgattggc tgatgtttt acgaggagc aacgagccg aagcattgg ctcaaacca attctatta ttttcaaat ttctgattt tgggcaaat tagaagcag cccaattgg caatcaata aaattcctt acgattcta tagagaaga tacgcagcg aagagcaga ttcatcaga ttgttaaga attatttga gttataaag cgtatttgg atgcacatg aggcgttta acgagtggt ttagacagt ataggaata agccgatgc caccacctg aactgcccg aaaatgcac aatttactg aaaaagttg ctcataatc ataaagtct cgcagttta atgccgaag cggaacggt ggaaattga cgacatcgc attcagcaa aaagggcca aaactttat tatataatg tccttttgt ttcctgaag ggtggcttg aaggaggag acgatgctg agtatctgc gatcaattt gtattcctc agattgttt tactattgt attcggttt taaatgagt ctggactgc acgaagaag cggtgcagt tggccgatt tagtggcgg ccaagaaat acggacttt acaaggtgt attctaaag agaaattgg cagagattt tggagaaga tttgtgaaa gttctgtgg cgcttctca acgcgaaga aagacccgt ttggaagct cctcaagtg tttaaaata attttctga ataaattcg ttttttcta a
