Status
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Gene information
General information
TC011645 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15947553..15951091
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011645
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01872

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011645 RA
      MDNDLERSV RLLEDALQT PTKGLFKVS TATPKREKP 
      NLSFTINPE DLTGLEYGS VYQNTLSLF VDDDKTVTG 
      SVIQAGTPW KNTVNNLYT EFADILQGS TGGLDILDV 
      AEDLARCCS DALAVVKSL KAKVATSEL AEEKWLENE 
      RNNWRLLYV LYQDRIQAQ NHSKEGFEE DFGSSEKNC 
      VCELFKREN LVRESQLVI DWLERNALE KGDPILMHS 
      DWTYGWEHT LHQLLAPDA IAIGSNVDI IKNIHPDAP 
      QFQRLSLHH LDYDNDKKL CQRIFMEIR CGNLEGAQE 
      LCYNSGYPW RAAMLEGWK LFHNPNLET SLEEYKDVE 
      GNQRRDLWK SCALDYCHR SNLNIYEKA AIGSLCGFL 
      EGILPVCTT WEDHLWAYM RTMVDIRVE SEIRDSIIK 
      NYIPLPEEY WEQKKSLSE IFTILEASK NIDIAKEAH 
      EIDHVIQKL VILDEIPTL IRLLDQWSE DLSVTTQSL 
      RFYAHLVLF FESVGDSTH RKTCEKIVE TYIERLSEM 
      NETQLVAFY VSKLGKSKQ IQLYAKHCE KLLSTEQRK 
      NALTYAEDF SLDVFAITQ QIVEIITSR PSESELPGN 
      LQGKITEID ELKISAIDW LMFYEEQRA EALAQTNSI 
      IFKFLILGK LEAAQLAIN KIPYDSIEK IRSEEQIHQ 
      IVKNYLSYK AYLDAHEAF NEWFRQYRN KPMPPPELP 
      ENAQFTEKV AHNHKVSQF NAEAERWKL TTSHSAKRA 
      KTLLYNVLL FPEGWLEGG DDAEYLRSI CIPQIVLLL 
      YSVLNESGL HEEAVQLAD LVAAKKYGL YKVYSKEKL 
      AEILEKICE SSVALLNAK KDPFGSSSS V
    CDS sequence
    • >TC011645 RA
      atggataat gatttggaa cggtcggtg cgtcttctc 
      gaagacgct ctccagacc cccacaaaa gggctattt 
      aaggtctcg accgcaact ccaaagcgt gaaaaacct 
      aacctcagc tttacaata aaccccgaa gacttaacc 
      ggcctggaa tatggaagt gtttatcaa aacaccttg 
      tctttattt gtcgatgat gacaaaacg gtcaccggg 
      tcggtgatc caagcagga actccatgg aaaaacact 
      gttaataat ttatataca gagtttgcg gatatcctg 
      caggggtcc accggaggc cttgatatc ctggacgtg 
      gccgaagac ttggcgagg tgttgtagt gacgctttg 
      gcggtggtt aagtcactg aaagccaaa gtggccact 
      agtgaatta gccgaagag aaatggctc gaaaatgag 
      cggaataat tggagactg ctgtatgtc ctgtaccag 
      gatcggatc caggcccag aaccacagt aaggaaggg 
      tttgaggag gatttcggg agcagtgaa aagaactgt 
      gtgtgcgaa ctgttcaaa cgagagaat ttggttagg 
      gaaagccag ttggtgatc gattggctc gagaggaac 
      gctctggag aagggggac cccatttta atgcactca 
      gactggacg tatggatgg gaacacact ttgcatcaa 
      ttgctagca cctgatgct attgcaatt gggagtaat 
      gtggatatt atcaagaac atacatcca gatgcgcca 
      cagtttcag cgcttatcg ctgcaccac ttggattac 
      gacaatgac aaaaaacta tgtcagaga atttttatg 
      gaaattagg tgcggcaac cttgagggc gcccaggaa 
      ttatgttac aacagtggt tatccatgg cgcgctgcg 
      atgttagaa ggctggaag ctttttcat aatcccaac 
      ctggaaaca agtcttgaa gagtataaa gacgtagaa 
      gggaaccaa aggcgggat ttgtggaaa tcttgcgca 
      ttagattat tgtcatagg tcaaacttg aacatttat 
      gaaaaagcc gccattggt agtctttgt ggtttcttg 
      gagggcatt ttgcccgtt tgtaccaca tgggaggac 
      cacctgtgg gcctacatg cgaaccatg gtcgatatt 
      cgtgttgaa tcggaaatt cgcgactca atcattaaa 
      aactacata ccactacct gaagaatat tgggaacaa 
      aagaaatca ttaagtgaa atttttaca attttggaa 
      gcctcgaaa aatattgac attgcaaaa gaagcgcat 
      gaaattgat catgtaatt cagaaactt gtcatttta 
      gacgaaatt cccacactg atacgcctc ctagatcag 
      tggagtgaa gatctaagt gttacgaca caatctctt 
      cgattttat gctcactta gttttgttt tttgaaagc 
      gtcggagac tccactcat agaaaaacg tgtgaaaaa 
      atcgtcgaa acatatatc gagcgtttg agcgaaatg 
      aacgaaacc cagcttgtg gccttctac gtgagcaag 
      ctgggaaaa tccaaacaa atccaactt tacgccaaa 
      cactgcgaa aaactgctt tcgactgag caacgcaaa 
      aacgccctt acttacgct gaagatttc agtttggac 
      gtatttgct ataacgcaa caaatcgtc gaaattata 
      acaagccga ccgtctgaa tccgaactt cccggtaat 
      ctccaaggt aaaatcacc gaaatcgac gaactcaaa 
      atctccgct attgattgg ctgatgttt tacgaggag 
      caacgagcc gaagcattg gctcaaacc aattctatt 
      attttcaaa tttctgatt ttgggcaaa ttagaagca 
      gcccaattg gcaatcaat aaaattcct tacgattct 
      atagagaag atacgcagc gaagagcag attcatcag 
      attgttaag aattatttg agttataaa gcgtatttg 
      gatgcacat gaggcgttt aacgagtgg tttagacag 
      tataggaat aagccgatg ccaccacct gaactgccc 
      gaaaatgca caatttact gaaaaagtt gctcataat 
      cataaagtc tcgcagttt aatgccgaa gcggaacgg 
      tggaaattg acgacatcg cattcagca aaaagggcc 
      aaaacttta ttatataat gtccttttg tttcctgaa 
      gggtggctt gaaggagga gacgatgct gagtatctg 
      cgatcaatt tgtattcct cagattgtt ttactattg 
      tattcggtt ttaaatgag tctggactg cacgaagaa 
      gcggtgcag ttggccgat ttagtggcg gccaagaaa 
      tacggactt tacaaggtg tattctaaa gagaaattg 
      gcagagatt ttggagaag atttgtgaa agttctgtg 
      gcgcttctc aacgcgaag aaagacccg tttggaagc 
      tcctcaagt gtttaa
    mRNA sequence
    • >TC011645 RA
      tcaacactt ttttcacca attttcctt caatttgaa 
      caaaaaaca cgataaaca caaacagac gcgataatt 
      tgaaacaaa tgacgatcg aggaaaaga aaaactgtt 
      accaacctg aatctaggg actttgcaa cgctctgag 
      gcaacgcta gacttgttt tttgaggtt atgcaacgg 
      ctttttgtg tgtgttttt tattaaaaa tggataatg 
      atttggaac ggtcggtgc gtcttctcg aagacgctc 
      tccagaccc ccacaaaag ggctattta aggtctcga 
      ccgcaactc caaagcgtg aaaaaccta acctcagct 
      ttacaataa accccgaag acttaaccg gcctggaat 
      atggaagtg tttatcaaa acaccttgt ctttatttg 
      tcgatgatg acaaaacgg tcaccgggt cggtgatcc 
      aagcaggaa ctccatgga aaaacactg ttaataatt 
      tatatacag agtttgcgg atatcctgc aggggtcca 
      ccggaggcc ttgatatcc tggacgtgg ccgaagact 
      tggcgaggt gttgtagtg acgctttgg cggtggtta 
      agtcactga aagccaaag tggccacta gtgaattag 
      ccgaagaga aatggctcg aaaatgagc ggaataatt 
      ggagactgc tgtatgtcc tgtaccagg atcggatcc 
      aggcccaga accacagta aggaagggt ttgaggagg 
      atttcggga gcagtgaaa agaactgtg tgtgcgaac 
      tgttcaaac gagagaatt tggttaggg aaagccagt 
      tggtgatcg attggctcg agaggaacg ctctggaga 
      agggggacc ccattttaa tgcactcag actggacgt 
      atggatggg aacacactt tgcatcaat tgctagcac 
      ctgatgcta ttgcaattg ggagtaatg tggatatta 
      tcaagaaca tacatccag atgcgccac agtttcagc 
      gcttatcgc tgcaccact tggattacg acaatgaca 
      aaaaactat gtcagagaa tttttatgg aaattaggt 
      gcggcaacc ttgagggcg cccaggaat tatgttaca 
      acagtggtt atccatggc gcgctgcga tgttagaag 
      gctggaagc tttttcata atcccaacc tggaaacaa 
      gtcttgaag agtataaag acgtagaag ggaaccaaa 
      ggcgggatt tgtggaaat cttgcgcat tagattatt 
      gtcataggt caaacttga acatttatg aaaaagccg 
      ccattggta gtctttgtg gtttcttgg agggcattt 
      tgcccgttt gtaccacat gggaggacc acctgtggg 
      cctacatgc gaaccatgg tcgatattc gtgttgaat 
      cggaaattc gcgactcaa tcattaaaa actacatac 
      cactacctg aagaatatt gggaacaaa agaaatcat 
      taagtgaaa tttttacaa ttttggaag cctcgaaaa 
      atattgaca ttgcaaaag aagcgcatg aaattgatc 
      atgtaattc agaaacttg tcattttag acgaaattc 
      ccacactga tacgcctcc tagatcagt ggagtgaag 
      atctaagtg ttacgacac aatctcttc gattttatg 
      ctcacttag ttttgtttt ttgaaagcg tcggagact 
      ccactcata gaaaaacgt gtgaaaaaa tcgtcgaaa 
      catatatcg agcgtttga gcgaaatga acgaaaccc 
      agcttgtgg ccttctacg tgagcaagc tgggaaaat 
      ccaaacaaa tccaacttt acgccaaac actgcgaaa 
      aactgcttt cgactgagc aacgcaaaa acgccctta 
      cttacgctg aagatttca gtttggacg tatttgcta 
      taacgcaac aaatcgtcg aaattataa caagccgac 
      cgtctgaat ccgaacttc ccggtaatc tccaaggta 
      aaatcaccg aaatcgacg aactcaaaa tctccgcta 
      ttgattggc tgatgtttt acgaggagc aacgagccg 
      aagcattgg ctcaaacca attctatta ttttcaaat 
      ttctgattt tgggcaaat tagaagcag cccaattgg 
      caatcaata aaattcctt acgattcta tagagaaga 
      tacgcagcg aagagcaga ttcatcaga ttgttaaga 
      attatttga gttataaag cgtatttgg atgcacatg 
      aggcgttta acgagtggt ttagacagt ataggaata 
      agccgatgc caccacctg aactgcccg aaaatgcac 
      aatttactg aaaaagttg ctcataatc ataaagtct 
      cgcagttta atgccgaag cggaacggt ggaaattga 
      cgacatcgc attcagcaa aaagggcca aaactttat 
      tatataatg tccttttgt ttcctgaag ggtggcttg 
      aaggaggag acgatgctg agtatctgc gatcaattt 
      gtattcctc agattgttt tactattgt attcggttt 
      taaatgagt ctggactgc acgaagaag cggtgcagt 
      tggccgatt tagtggcgg ccaagaaat acggacttt 
      acaaggtgt attctaaag agaaattgg cagagattt 
      tggagaaga tttgtgaaa gttctgtgg cgcttctca 
      acgcgaaga aagacccgt ttggaagct cctcaagtg 
      tttaaaata attttctga ataaattcg ttttttcta 
      a
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