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Gene information
General information
TC011645 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15947553..15951091
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011645
    iBeetle screen iB_01872

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    Pupal morphology
    remarks: later than schedule,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011645 RA
      MDNDLERSV RLLEDALQT PTKGLFKVS TATPKREKP 
NLSFTINPE DLTGLEYGS VYQNTLSLF VDDDKTVTG 
SVIQAGTPW KNTVNNLYT EFADILQGS TGGLDILDV 
AEDLARCCS DALAVVKSL KAKVATSEL AEEKWLENE 
RNNWRLLYV LYQDRIQAQ NHSKEGFEE DFGSSEKNC 
VCELFKREN LVRESQLVI DWLERNALE KGDPILMHS 
DWTYGWEHT LHQLLAPDA IAIGSNVDI IKNIHPDAP 
QFQRLSLHH LDYDNDKKL CQRIFMEIR CGNLEGAQE 
LCYNSGYPW RAAMLEGWK LFHNPNLET SLEEYKDVE 
GNQRRDLWK SCALDYCHR SNLNIYEKA AIGSLCGFL 
EGILPVCTT WEDHLWAYM RTMVDIRVE SEIRDSIIK 
NYIPLPEEY WEQKKSLSE IFTILEASK NIDIAKEAH 
EIDHVIQKL VILDEIPTL IRLLDQWSE DLSVTTQSL 
RFYAHLVLF FESVGDSTH RKTCEKIVE TYIERLSEM 
NETQLVAFY VSKLGKSKQ IQLYAKHCE KLLSTEQRK 
NALTYAEDF SLDVFAITQ QIVEIITSR PSESELPGN 
LQGKITEID ELKISAIDW LMFYEEQRA EALAQTNSI 
IFKFLILGK LEAAQLAIN KIPYDSIEK IRSEEQIHQ 
IVKNYLSYK AYLDAHEAF NEWFRQYRN KPMPPPELP 
ENAQFTEKV AHNHKVSQF NAEAERWKL TTSHSAKRA 
KTLLYNVLL FPEGWLEGG DDAEYLRSI CIPQIVLLL 
YSVLNESGL HEEAVQLAD LVAAKKYGL YKVYSKEKL 
AEILEKICE SSVALLNAK KDPFGSSSS V
    CDS sequence
    • >TC011645 RA
      atggataat gatttggaa cggtcggtg cgtcttctc 
gaagacgct ctccagacc cccacaaaa gggctattt 
aaggtctcg accgcaact ccaaagcgt gaaaaacct 
aacctcagc tttacaata aaccccgaa gacttaacc 
ggcctggaa tatggaagt gtttatcaa aacaccttg 
tctttattt gtcgatgat gacaaaacg gtcaccggg 
tcggtgatc caagcagga actccatgg aaaaacact 
gttaataat ttatataca gagtttgcg gatatcctg 
caggggtcc accggaggc cttgatatc ctggacgtg 
gccgaagac ttggcgagg tgttgtagt gacgctttg 
gcggtggtt aagtcactg aaagccaaa gtggccact 
agtgaatta gccgaagag aaatggctc gaaaatgag 
cggaataat tggagactg ctgtatgtc ctgtaccag 
gatcggatc caggcccag aaccacagt aaggaaggg 
tttgaggag gatttcggg agcagtgaa aagaactgt 
gtgtgcgaa ctgttcaaa cgagagaat ttggttagg 
gaaagccag ttggtgatc gattggctc gagaggaac 
gctctggag aagggggac cccatttta atgcactca 
gactggacg tatggatgg gaacacact ttgcatcaa 
ttgctagca cctgatgct attgcaatt gggagtaat 
gtggatatt atcaagaac atacatcca gatgcgcca 
cagtttcag cgcttatcg ctgcaccac ttggattac 
gacaatgac aaaaaacta tgtcagaga atttttatg 
gaaattagg tgcggcaac cttgagggc gcccaggaa 
ttatgttac aacagtggt tatccatgg cgcgctgcg 
atgttagaa ggctggaag ctttttcat aatcccaac 
ctggaaaca agtcttgaa gagtataaa gacgtagaa 
gggaaccaa aggcgggat ttgtggaaa tcttgcgca 
ttagattat tgtcatagg tcaaacttg aacatttat 
gaaaaagcc gccattggt agtctttgt ggtttcttg 
gagggcatt ttgcccgtt tgtaccaca tgggaggac 
cacctgtgg gcctacatg cgaaccatg gtcgatatt 
cgtgttgaa tcggaaatt cgcgactca atcattaaa 
aactacata ccactacct gaagaatat tgggaacaa 
aagaaatca ttaagtgaa atttttaca attttggaa 
gcctcgaaa aatattgac attgcaaaa gaagcgcat 
gaaattgat catgtaatt cagaaactt gtcatttta 
gacgaaatt cccacactg atacgcctc ctagatcag 
tggagtgaa gatctaagt gttacgaca caatctctt 
cgattttat gctcactta gttttgttt tttgaaagc 
gtcggagac tccactcat agaaaaacg tgtgaaaaa 
atcgtcgaa acatatatc gagcgtttg agcgaaatg 
aacgaaacc cagcttgtg gccttctac gtgagcaag 
ctgggaaaa tccaaacaa atccaactt tacgccaaa 
cactgcgaa aaactgctt tcgactgag caacgcaaa 
aacgccctt acttacgct gaagatttc agtttggac 
gtatttgct ataacgcaa caaatcgtc gaaattata 
acaagccga ccgtctgaa tccgaactt cccggtaat 
ctccaaggt aaaatcacc gaaatcgac gaactcaaa 
atctccgct attgattgg ctgatgttt tacgaggag 
caacgagcc gaagcattg gctcaaacc aattctatt 
attttcaaa tttctgatt ttgggcaaa ttagaagca 
gcccaattg gcaatcaat aaaattcct tacgattct 
atagagaag atacgcagc gaagagcag attcatcag 
attgttaag aattatttg agttataaa gcgtatttg 
gatgcacat gaggcgttt aacgagtgg tttagacag 
tataggaat aagccgatg ccaccacct gaactgccc 
gaaaatgca caatttact gaaaaagtt gctcataat 
cataaagtc tcgcagttt aatgccgaa gcggaacgg 
tggaaattg acgacatcg cattcagca aaaagggcc 
aaaacttta ttatataat gtccttttg tttcctgaa 
gggtggctt gaaggagga gacgatgct gagtatctg 
cgatcaatt tgtattcct cagattgtt ttactattg 
tattcggtt ttaaatgag tctggactg cacgaagaa 
gcggtgcag ttggccgat ttagtggcg gccaagaaa 
tacggactt tacaaggtg tattctaaa gagaaattg 
gcagagatt ttggagaag atttgtgaa agttctgtg 
gcgcttctc aacgcgaag aaagacccg tttggaagc 
tcctcaagt gtttaa
    mRNA sequence
    • >TC011645 RA
      tcaacactt ttttcacca attttcctt caatttgaa 
caaaaaaca cgataaaca caaacagac gcgataatt 
tgaaacaaa tgacgatcg aggaaaaga aaaactgtt 
accaacctg aatctaggg actttgcaa cgctctgag 
gcaacgcta gacttgttt tttgaggtt atgcaacgg 
ctttttgtg tgtgttttt tattaaaaa tggataatg 
atttggaac ggtcggtgc gtcttctcg aagacgctc 
tccagaccc ccacaaaag ggctattta aggtctcga 
ccgcaactc caaagcgtg aaaaaccta acctcagct 
ttacaataa accccgaag acttaaccg gcctggaat 
atggaagtg tttatcaaa acaccttgt ctttatttg 
tcgatgatg acaaaacgg tcaccgggt cggtgatcc 
aagcaggaa ctccatgga aaaacactg ttaataatt 
tatatacag agtttgcgg atatcctgc aggggtcca 
ccggaggcc ttgatatcc tggacgtgg ccgaagact 
tggcgaggt gttgtagtg acgctttgg cggtggtta 
agtcactga aagccaaag tggccacta gtgaattag 
ccgaagaga aatggctcg aaaatgagc ggaataatt 
ggagactgc tgtatgtcc tgtaccagg atcggatcc 
aggcccaga accacagta aggaagggt ttgaggagg 
atttcggga gcagtgaaa agaactgtg tgtgcgaac 
tgttcaaac gagagaatt tggttaggg aaagccagt 
tggtgatcg attggctcg agaggaacg ctctggaga 
agggggacc ccattttaa tgcactcag actggacgt 
atggatggg aacacactt tgcatcaat tgctagcac 
ctgatgcta ttgcaattg ggagtaatg tggatatta 
tcaagaaca tacatccag atgcgccac agtttcagc 
gcttatcgc tgcaccact tggattacg acaatgaca 
aaaaactat gtcagagaa tttttatgg aaattaggt 
gcggcaacc ttgagggcg cccaggaat tatgttaca 
acagtggtt atccatggc gcgctgcga tgttagaag 
gctggaagc tttttcata atcccaacc tggaaacaa 
gtcttgaag agtataaag acgtagaag ggaaccaaa 
ggcgggatt tgtggaaat cttgcgcat tagattatt 
gtcataggt caaacttga acatttatg aaaaagccg 
ccattggta gtctttgtg gtttcttgg agggcattt 
tgcccgttt gtaccacat gggaggacc acctgtggg 
cctacatgc gaaccatgg tcgatattc gtgttgaat 
cggaaattc gcgactcaa tcattaaaa actacatac 
cactacctg aagaatatt gggaacaaa agaaatcat 
taagtgaaa tttttacaa ttttggaag cctcgaaaa 
atattgaca ttgcaaaag aagcgcatg aaattgatc 
atgtaattc agaaacttg tcattttag acgaaattc 
ccacactga tacgcctcc tagatcagt ggagtgaag 
atctaagtg ttacgacac aatctcttc gattttatg 
ctcacttag ttttgtttt ttgaaagcg tcggagact 
ccactcata gaaaaacgt gtgaaaaaa tcgtcgaaa 
catatatcg agcgtttga gcgaaatga acgaaaccc 
agcttgtgg ccttctacg tgagcaagc tgggaaaat 
ccaaacaaa tccaacttt acgccaaac actgcgaaa 
aactgcttt cgactgagc aacgcaaaa acgccctta 
cttacgctg aagatttca gtttggacg tatttgcta 
taacgcaac aaatcgtcg aaattataa caagccgac 
cgtctgaat ccgaacttc ccggtaatc tccaaggta 
aaatcaccg aaatcgacg aactcaaaa tctccgcta 
ttgattggc tgatgtttt acgaggagc aacgagccg 
aagcattgg ctcaaacca attctatta ttttcaaat 
ttctgattt tgggcaaat tagaagcag cccaattgg 
caatcaata aaattcctt acgattcta tagagaaga 
tacgcagcg aagagcaga ttcatcaga ttgttaaga 
attatttga gttataaag cgtatttgg atgcacatg 
aggcgttta acgagtggt ttagacagt ataggaata 
agccgatgc caccacctg aactgcccg aaaatgcac 
aatttactg aaaaagttg ctcataatc ataaagtct 
cgcagttta atgccgaag cggaacggt ggaaattga 
cgacatcgc attcagcaa aaagggcca aaactttat 
tatataatg tccttttgt ttcctgaag ggtggcttg 
aaggaggag acgatgctg agtatctgc gatcaattt 
gtattcctc agattgttt tactattgt attcggttt 
taaatgagt ctggactgc acgaagaag cggtgcagt 
tggccgatt tagtggcgg ccaagaaat acggacttt 
acaaggtgt attctaaag agaaattgg cagagattt 
tggagaaga tttgtgaaa gttctgtgg cgcttctca 
acgcgaaga aagacccgt ttggaagct cctcaagtg 
tttaaaata attttctga ataaattcg ttttttcta 
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    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY