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Gene information
General information
TC011641 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15887623..15933912
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011641
    iBeetle screen iB_01871

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    remarks: later than schedule,
    Pupal morphology
    remarks: later than schedule,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011641 RA
      MALSNLTAG PAQAVEDFL LQVENATLL FNATTANAT 
      VALHYPSGY TLPQIVVAS VVVTMLMIV VVVGNMLVI 
      IAITTEKAL KNIQNWFIA SLAVADFFL GLVIMPFSL 
      ANELMGYWI FGMWWCDVH SAMDVLLST ASIMNLCLI 
      SLDRYWSIT QAVEYLKKR TPVRAVVMI AAVWVLSAL 
      ICIPPLLGW KVERTPDEQ YPKCQLSEE LGYVLYSAL 
      GSFYIPSCI MVFVYIRIY YAAKARARR GIRKPPRRQ 
      PQDAVTSFS VQKNGDGDG AATAGASVD RNSNTSPRD 
      GDRQIATIE AIPRPMPIP TVTCDLASD ISTSDAGGE 
      IVAETLDQK DTLKVFSSA QVVRNPLAQ SQFRGSTLS 
      VNGELQQQA AAMSRMRQP SMGIDTDMV SEFDPSSSD 
      SGVVSRCAV VKPLKLRLC KPIFGRKTA KNRRTAKDK 
      ASSSKEHMV EEVRVQKPR DPEREKRRI ARKKEKRAT 
      LILGLIMGS FIACWLPFF FMYILRLAY DIPGIAFST 
      AFWLGYMNS ALNPVIYTI FNKDFRRAF RRILFK
    • >TC011641 RB
      MALSNLTAG PAQAVEDFL LQVENATLL FNATTANAT 
      VALHYPSGY TLPQIVVAS VVVTMLMIV VVVGNMLVI 
      IAITTEKAL KNIQNWFIA SLAVADFFL GLVIMPFSL 
      ANELMGYWI FGMWWCDVH SAMDVLLST ASIMNLCLI 
      SLDRYWSIT QAVEYLKKR TPVRAVVMI AAVWVLSAL 
      ICIPPLLGW KVERTPDEQ YPKCQLSEE LGYVLYSAL 
      GSFYIPSCI MVFVYIRIY YAAKARARR GIRKPPRRQ 
      PQDAVTSFS VQKNGDGDG AATAGASVD RNSNTSPRD 
      GDRQIATIE AIPRPMPIP TVTCDLASD ISTSDAGGE 
      IVAETLDQK DTLKVCTID SDGPKCQPQ DDAQVFSSA 
      QVVRNPLAQ SQFRGSTLS VNGELQQQA AAMSRMRQP 
      SMGIDTDMV SEFDPSSSD SGVVSRCAV VKPLKLRLC 
      KPIFGRKTA KNRRTAKDK ASSSKEHMV EEVRVQKPR 
      DPEREKRRI ARKKEKRAT LILGLIMGS FIACWLPFF 
      FMYILRLAY DIPGIAFST AFWLGYMNS ALNPVIYTI 
      FNKDFRRAF RRILFK
    CDS sequence
    • >TC011641 RA
      atggcgctc agcaacctg acggcaggg ccggcccaa 
      gccgtcgag gacttcctt ctgcaagtg gaaaacgcg 
      acgctgctc tttaacgcc accaccgcc aacgccacc 
      gtcgccctc cactacccc agcggctac actctgccc 
      cagatcgtg gtcgcttcc gtcgttgtc acgatgctg 
      atgatcgtc gtcgtcgtg ggcaacatg ctggtcatc 
      attgccatc accacggag aaggcgctc aaaaacata 
      caaaactgg tttattgct tcgttagcc gtcgccgac 
      ttcttctta gggctggtg attatgccg ttttcgctc 
      gccaacgaa ctcatgggt tattggatt ttcgggatg 
      tggtggtgc gatgtgcat tccgccatg gatgtgctg 
      ctgagcact gcgtccatt atgaacttg tgtctcatt 
      tcgttggat cgctattgg agtatcact caagcggtt 
      gaatatctc aagaagcgc actccggta cgtgctgtg 
      gtcatgatt gccgccgtt tgggttctc tctgccctc 
      atatgcatc cctccgttg ctcggctgg aaggtggag 
      cgcactcca gacgaacag tatcccaag tgtcagctg 
      agtgaagaa ctgggttac gtgctgtat tcggcactg 
      ggttcgttc tacatccca tcgtgcata atggtattt 
      gtatatatc cgaatttac tacgcggcg aaagctcgt 
      gctcgacgc ggcatccgg aagccgcct cgacgccaa 
      ccccaagac gccgtcacc agtttcagt gtacagaag 
      aacggagat ggcgacggc gccgcgacg gcaggcgct 
      tccgtcgat aggaacagc aacacgtcg ccgagggac 
      ggagacaga caaatcgcc accatcgag gcgattcca 
      cgcccaatg cccattcca acagtcacg tgcgattta 
      gcatcggat atttcgacg agcgacgcc ggaggagaa 
      atcgtcgcc gagacgctt gatcagaag gatacgctc 
      aaggtgttc agcagtgcc caggtggtc cgaaaccct 
      ctggcccag agccaattc cgaggctct acgttatcg 
      gtaaacggc gaattgcaa cagcaagct gctgccatg 
      tctcgtatg cgtcaacca tcgatgggc atcgacacg 
      gatatggtg agcgaattt gatccgagc agttcggac 
      tctggtgta gtgtcccgg tgtgcggtg gtgaaaccg 
      ttaaaactc cgtctgtgc aaaccgata ttcggtcgc 
      aaaacggcg aaaaaccga aggaccgcc aaagacaaa 
      gcgagctcg agcaaagag cacatggtg gaggaggtg 
      cgcgtccag aagcccaga gacccagaa cgagagaag 
      cgacgcatc gcgaggaaa aaggaaaaa agggcaaca 
      ctcattctg ggtctcatt atgggctcg tttatcgcc 
      tgctggttg cccttcttc ttcatgtac atcctgcgg 
      ctggcctac gatatcccg gggatcgcc ttctccacg 
      gccttttgg ttggggtac atgaactcg gcgctgaac 
      ccggttatc tacaccatc ttcaacaag gacttccgg 
      agggccttc aggagaatc ctgttcaag tga
    • >TC011641 RB
      atggcgctc agcaacctg acggcaggg ccggcccaa 
      gccgtcgag gacttcctt ctgcaagtg gaaaacgcg 
      acgctgctc tttaacgcc accaccgcc aacgccacc 
      gtcgccctc cactacccc agcggctac actctgccc 
      cagatcgtg gtcgcttcc gtcgttgtc acgatgctg 
      atgatcgtc gtcgtcgtg ggcaacatg ctggtcatc 
      attgccatc accacggag aaggcgctc aaaaacata 
      caaaactgg tttattgct tcgttagcc gtcgccgac 
      ttcttctta gggctggtg attatgccg ttttcgctc 
      gccaacgaa ctcatgggt tattggatt ttcgggatg 
      tggtggtgc gatgtgcat tccgccatg gatgtgctg 
      ctgagcact gcgtccatt atgaacttg tgtctcatt 
      tcgttggat cgctattgg agtatcact caagcggtt 
      gaatatctc aagaagcgc actccggta cgtgctgtg 
      gtcatgatt gccgccgtt tgggttctc tctgccctc 
      atatgcatc cctccgttg ctcggctgg aaggtggag 
      cgcactcca gacgaacag tatcccaag tgtcagctg 
      agtgaagaa ctgggttac gtgctgtat tcggcactg 
      ggttcgttc tacatccca tcgtgcata atggtattt 
      gtatatatc cgaatttac tacgcggcg aaagctcgt 
      gctcgacgc ggcatccgg aagccgcct cgacgccaa 
      ccccaagac gccgtcacc agtttcagt gtacagaag 
      aacggagat ggcgacggc gccgcgacg gcaggcgct 
      tccgtcgat aggaacagc aacacgtcg ccgagggac 
      ggagacaga caaatcgcc accatcgag gcgattcca 
      cgcccaatg cccattcca acagtcacg tgcgattta 
      gcatcggat atttcgacg agcgacgcc ggaggagaa 
      atcgtcgcc gagacgctt gatcagaag gatacgctc 
      aaggtttgt acgattgac agtgacgga ccgaaatgc 
      caaccgcag gacgacgct caagtgttc agcagtgcc 
      caggtggtc cgaaaccct ctggcccag agccaattc 
      cgaggctct acgttatcg gtaaacggc gaattgcaa 
      cagcaagct gctgccatg tctcgtatg cgtcaacca 
      tcgatgggc atcgacacg gatatggtg agcgaattt 
      gatccgagc agttcggac tctggtgta gtgtcccgg 
      tgtgcggtg gtgaaaccg ttaaaactc cgtctgtgc 
      aaaccgata ttcggtcgc aaaacggcg aaaaaccga 
      aggaccgcc aaagacaaa gcgagctcg agcaaagag 
      cacatggtg gaggaggtg cgcgtccag aagcccaga 
      gacccagaa cgagagaag cgacgcatc gcgaggaaa 
      aaggaaaaa agggcaaca ctcattctg ggtctcatt 
      atgggctcg tttatcgcc tgctggttg cccttcttc 
      ttcatgtac atcctgcgg ctggcctac gatatcccg 
      gggatcgcc ttctccacg gccttttgg ttggggtac 
      atgaactcg gcgctgaac ccggttatc tacaccatc 
      ttcaacaag gacttccgg agggccttc aggagaatc 
      ctgttcaag tga
    mRNA sequence
    • >TC011641 RA
      tccgggagg aggctcaca cagtgtgcg gtcgtggag 
      caaggcggt aacatgcca agcgagctc cgagtcgaa 
      agctccgcc gaggacgac gacgaagac gccgcgccg 
      ccgacgctt cggcctcgg ctccccatc tacaccata 
      ttctaaact agctagtgc tctctctaa ttctctaat 
      taaaaattt aataaaaat tgttgcgtt cgtttcaca 
      ttatatcgc cgatacacg ggctgcttc gggtacacg 
      ggtattgtt gtacatagt gactaggga ccaaccaaa 
      tggcgctca gcaacctga cggcagggc cggcccaag 
      ccgtcgagg acttccttc tgcaagtgg aaaacgcga 
      cgctgctct ttaacgcca ccaccgcca acgccaccg 
      tcgccctcc actacccca gcggctaca ctctgcccc 
      agatcgtgg tcgcttccg tcgttgtca cgatgctga 
      tgatcgtcg tcgtcgtgg gcaacatgc tggtcatca 
      ttgccatca ccacggaga aggcgctca aaaacatac 
      aaaactggt ttattgctt cgttagccg tcgccgact 
      tcttcttag ggctggtga ttatgccgt tttcgctcg 
      ccaacgaac tcatgggtt attggattt tcgggatgt 
      ggtggtgcg atgtgcatt ccgccatgg atgtgctgc 
      tgagcactg cgtccatta tgaacttgt gtctcattt 
      cgttggatc gctattgga gtatcactc aagcggttg 
      aatatctca agaagcgca ctccggtac gtgctgtgg 
      tcatgattg ccgccgttt gggttctct ctgccctca 
      tatgcatcc ctccgttgc tcggctgga aggtggagc 
      gcactccag acgaacagt atcccaagt gtcagctga 
      gtgaagaac tgggttacg tgctgtatt cggcactgg 
      gttcgttct acatcccat cgtgcataa tggtatttg 
      tatatatcc gaatttact acgcggcga aagctcgtg 
      ctcgacgcg gcatccgga agccgcctc gacgccaac 
      cccaagacg ccgtcacca gtttcagtg tacagaaga 
      acggagatg gcgacggcg ccgcgacgg caggcgctt 
      ccgtcgata ggaacagca acacgtcgc cgagggacg 
      gagacagac aaatcgcca ccatcgagg cgattccac 
      gcccaatgc ccattccaa cagtcacgt gcgatttag 
      catcggata tttcgacga gcgacgccg gaggagaaa 
      tcgtcgccg agacgcttg atcagaagg atacgctca 
      aggtgttca gcagtgccc aggtggtcc gaaaccctc 
      tggcccaga gccaattcc gaggctcta cgttatcgg 
      taaacggcg aattgcaac agcaagctg ctgccatgt 
      ctcgtatgc gtcaaccat cgatgggca tcgacacgg 
      atatggtga gcgaatttg atccgagca gttcggact 
      ctggtgtag tgtcccggt gtgcggtgg tgaaaccgt 
      taaaactcc gtctgtgca aaccgatat tcggtcgca 
      aaacggcga aaaaccgaa ggaccgcca aagacaaag 
      cgagctcga gcaaagagc acatggtgg aggaggtgc 
      gcgtccaga agcccagag acccagaac gagagaagc 
      gacgcatcg cgaggaaaa aggaaaaaa gggcaacac 
      tcattctgg gtctcatta tgggctcgt ttatcgcct 
      gctggttgc ccttcttct tcatgtaca tcctgcggc 
      tggcctacg atatcccgg ggatcgcct tctccacgg 
      ccttttggt tggggtaca tgaactcgg cgctgaacc 
      cggttatct acaccatct tcaacaagg acttccgga 
      gggccttca ggagaatcc tgttcaagt gatgtttgg 
      cttacgtgt gctgctatg aatgcgtca gccgacgga 
      tacggacgg cgcaatatc gcagcgcac caagtgccc 
      tctaccgac actgaactc tcgacaatc ctcctcacg 
      tggacatta ttatactct tcgatatac gtgtaaatt 
      gttatttct tgttaggta cggagcaat cgtcgtcaa 
      ctcgagcca gaaatcaaa atatagatg gcgccaata 
      cacttgttt taacaaaag tacaaaacg aatttttat 
      tctaaacat caaaagtat ggaaattgc cttaattaa 
      tgctatgta atattatat caaactgaa aattgtatc 
      gtatgtgta gtcagtcta cttccaaat aactccact 
      ctgtttaat ggaagcgat tttatctgt agcttttaa 
      ggtttttag gaaatgact cgagttttt attgtaata 
      tcaaaacgt tccgagttg gccacgatt gttatattt 
      tatttgact ctattgtaa taataaaat atggagcaa 
      tgactacca ctgtgataa tataacgct ttcattctg 
      gaatttatc gtaaatagt ctaaaagaa aacaaacta 
      ttatgtgtt tttaaaaaa atcttcgat gtttagaga 
      cttcatttc ttgtacata acttaatcg aaaagcgag 
      tacaagtta cccaacttt gaatccatg aagaaaaac 
      aacgtttac cagattcta ccctgcttt ttagcaaat 
      tgtaataca gcgtgtccc agcgatttg gaaaagtcc 
      atcattgtc ctttaaatg caaaattac ataccaaat 
      tagctgata ttaacccat agcatcacc tgaaattag 
      atacttcaa aaacgagtt acaaaacaa aaatatgtt 
      tttcaaaag gaaacaatc taggtttct tttgatctc 
      tatgctctt aaccaagcc cttcttgga ttttttcag 
      tttgcagcc caacactat aattgcata agcaaggac 
      attttctgt tccattgac accctattg ctacatcta 
      ataacaaca taagtttgt cttgtaact cagttttgg 
      actaaaaaa tcatttttg atgaagcaa tagatcatt 
      ggatcattg gtcaataat ggaattttt caattaact 
      gtatatgtt gaagggcgc tcctcagac caagtcgtt 
      tggaccaaa cctccactc ttcattgat tttcgtcgt 
      tgcgcgatg tcaaaattg acaagggac caatgaaac 
      cgtttttaa ttatgcgcg ttcgaacac ccgccgccg 
      ccgccgccg ccgccgccg ccacagcaa taataataa 
      taataataa tttccttgt gaacgttgg cgtttttcg 
      cttccaagt ccaagttgc gtaactttt cgtatttta 
      aatgttaat agaacgaat ggagcgagt gacaagttc 
      ggctatagt ttagacacc aattgtatc aaacctctg 
      gaggccgtc ggccgtcat ctcccttcc gttccgacc 
      atcaccctc tatcctttt ccaaaaaaa aaaaaataa 
      cagacgttg ccttggcga ggtctccag tggaagtct 
      tccacgagc ccgcggggt tgctctccg actcctgac 
      aaattcttg caacgaaac tatggtgct attgactgc 
      tcgccggtc ggctcaagg gtgtctcag gccctggag 
      ccggccctg tgcggcccc gcgaggcga tatcgaaac 
      tcgccctga gcccgcgat tttcgatct cgcgagctc 
      tttatcttg ttacatctc tagattaat tattgttat 
      ataaagatg aaaaaacca attataaac tataacact 
      gtcctatat ttgtgtgtg tacgttttt cgtgaaata 
      ttattaata aggatataa ataaatcaa tatggtact 
      g
    • >TC011641 RB
      tccgggagg aggctcaca cagtgtgcg gtcgtggag 
      caaggcggt aacatgcca agcgagctc cgagtcgaa 
      agctccgcc gaggacgac gacgaagac gccgcgccg 
      ccgacgctt cggcctcgg ctccccatc tacaccata 
      ttctaaact agctagtgc tctctctaa ttctctaat 
      taaaaattt aataaaaat tgttgcgtt cgtttcaca 
      ttatatcgc cgatacacg ggctgcttc gggtacacg 
      ggtattgtt gtacatagt gactaggga ccaaccaaa 
      tggcgctca gcaacctga cggcagggc cggcccaag 
      ccgtcgagg acttccttc tgcaagtgg aaaacgcga 
      cgctgctct ttaacgcca ccaccgcca acgccaccg 
      tcgccctcc actacccca gcggctaca ctctgcccc 
      agatcgtgg tcgcttccg tcgttgtca cgatgctga 
      tgatcgtcg tcgtcgtgg gcaacatgc tggtcatca 
      ttgccatca ccacggaga aggcgctca aaaacatac 
      aaaactggt ttattgctt cgttagccg tcgccgact 
      tcttcttag ggctggtga ttatgccgt tttcgctcg 
      ccaacgaac tcatgggtt attggattt tcgggatgt 
      ggtggtgcg atgtgcatt ccgccatgg atgtgctgc 
      tgagcactg cgtccatta tgaacttgt gtctcattt 
      cgttggatc gctattgga gtatcactc aagcggttg 
      aatatctca agaagcgca ctccggtac gtgctgtgg 
      tcatgattg ccgccgttt gggttctct ctgccctca 
      tatgcatcc ctccgttgc tcggctgga aggtggagc 
      gcactccag acgaacagt atcccaagt gtcagctga 
      gtgaagaac tgggttacg tgctgtatt cggcactgg 
      gttcgttct acatcccat cgtgcataa tggtatttg 
      tatatatcc gaatttact acgcggcga aagctcgtg 
      ctcgacgcg gcatccgga agccgcctc gacgccaac 
      cccaagacg ccgtcacca gtttcagtg tacagaaga 
      acggagatg gcgacggcg ccgcgacgg caggcgctt 
      ccgtcgata ggaacagca acacgtcgc cgagggacg 
      gagacagac aaatcgcca ccatcgagg cgattccac 
      gcccaatgc ccattccaa cagtcacgt gcgatttag 
      catcggata tttcgacga gcgacgccg gaggagaaa 
      tcgtcgccg agacgcttg atcagaagg atacgctca 
      aggtttgta cgattgaca gtgacggac cgaaatgcc 
      aaccgcagg acgacgctc aagtgttca gcagtgccc 
      aggtggtcc gaaaccctc tggcccaga gccaattcc 
      gaggctcta cgttatcgg taaacggcg aattgcaac 
      agcaagctg ctgccatgt ctcgtatgc gtcaaccat 
      cgatgggca tcgacacgg atatggtga gcgaatttg 
      atccgagca gttcggact ctggtgtag tgtcccggt 
      gtgcggtgg tgaaaccgt taaaactcc gtctgtgca 
      aaccgatat tcggtcgca aaacggcga aaaaccgaa 
      ggaccgcca aagacaaag cgagctcga gcaaagagc 
      acatggtgg aggaggtgc gcgtccaga agcccagag 
      acccagaac gagagaagc gacgcatcg cgaggaaaa 
      aggaaaaaa gggcaacac tcattctgg gtctcatta 
      tgggctcgt ttatcgcct gctggttgc ccttcttct 
      tcatgtaca tcctgcggc tggcctacg atatcccgg 
      ggatcgcct tctccacgg ccttttggt tggggtaca 
      tgaactcgg cgctgaacc cggttatct acaccatct 
      tcaacaagg acttccgga gggccttca ggagaatcc 
      tgttcaagt gatgtttgg cttacgtgt gctgctatg 
      aatgcgtca gccgacgga tacggacgg cgcaatatc 
      gcagcgcac caagtgccc tctaccgac actgaactc 
      tcgacaatc ctcctcacg tggacatta ttatactct 
      tcgatatac gtgtaaatt gttatttct tgttaggta 
      cggagcaat cgtcgtcaa ctcgagcca gaaatcaaa 
      atatagatg gcgccaata cacttgttt taacaaaag 
      tacaaaacg aatttttat tctaaacat caaaagtat 
      ggaaattgc cttaattaa tgctatgta atattatat 
      caaactgaa aattgtatc gtatgtgta gtcagtcta 
      cttccaaat aactccact ctgtttaat ggaagcgat 
      tttatctgt agcttttaa ggtttttag gaaatgact 
      cgagttttt attgtaata tcaaaacgt tccgagttg 
      gccacgatt gttatattt tatttgact ctattgtaa 
      taataaaat atggagcaa tgactacca ctgtgataa 
      tataacgct ttcattctg gaatttatc gtaaatagt 
      ctaaaagaa aacaaacta ttatgtgtt tttaaaaaa 
      atcttcgat gtttagaga cttcatttc ttgtacata 
      acttaatcg aaaagcgag tacaagtta cccaacttt 
      gaatccatg aagaaaaac aacgtttac cagattcta 
      ccctgcttt ttagcaaat tgtaataca gcgtgtccc 
      agcgatttg gaaaagtcc atcattgtc ctttaaatg 
      caaaattac ataccaaat tagctgata ttaacccat 
      agcatcacc tgaaattag atacttcaa aaacgagtt 
      acaaaacaa aaatatgtt tttcaaaag gaaacaatc 
      taggtttct tttgatctc tatgctctt aaccaagcc 
      cttcttgga ttttttcag tttgcagcc caacactat 
      aattgcata agcaaggac attttctgt tccattgac 
      accctattg ctacatcta ataacaaca taagtttgt 
      cttgtaact cagttttgg actaaaaaa tcatttttg 
      atgaagcaa tagatcatt ggatcattg gtcaataat 
      ggaattttt caattaact gtatatgtt gaagggcgc 
      tcctcagac caagtcgtt tggaccaaa cctccactc 
      ttcattgat tttcgtcgt tgcgcgatg tcaaaattg 
      acaagggac caatgaaac cgtttttaa ttatgcgcg 
      ttcgaacac ccgccgccg ccgccgccg ccgccgccg 
      ccacagcaa taataataa taataataa tttccttgt 
      gaacgttgg cgtttttcg cttccaagt ccaagttgc 
      gtaactttt cgtatttta aatgttaat agaacgaat 
      ggagcgagt gacaagttc ggctatagt ttagacacc 
      aattgtatc aaacctctg gaggccgtc ggccgtcat 
      ctcccttcc gttccgacc atcaccctc tatcctttt 
      ccaaaaaaa aaaaaataa cagacgttg ccttggcga 
      ggtctccag tggaagtct tccacgagc ccgcggggt 
      tgctctccg actcctgac aaattcttg caacgaaac 
      tatggtgct attgactgc tcgccggtc ggctcaagg 
      gtgtctcag gccctggag ccggccctg tgcggcccc 
      gcgaggcga tatcgaaac tcgccctga gcccgcgat 
      tttcgatct cgcgagctc tttatcttg ttacatctc 
      tagattaat tattgttat ataaagatg aaaaaacca 
      attataaac tataacact gtcctatat ttgtgtgtg 
      tacgttttt cgtgaaata ttattaata aggatataa 
      ataaatcaa tatggtact g
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