Status
Please wait ...
Gene information
General information
TC031254
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011610
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid67: 7490749..7525870
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031254
    iBeetle screen iB_01868
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva bristle mostlynot present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection


    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 9


    elytra shape irregular - Number of animals showing the phenotype: 4


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031254 RA
      METCVLIPQ EFSLCVRGP APRRTAEPE VSVWSNTSI 
      AQGSLCYPF QGTIRIDKL DVYGQLDDD DIRHRFGCY 
      DEISGSGSR RVRHCNWVR FVRVASSFN PAVNFVATK 
      VRGEPVYEA VKPISPDTE LLVYYLPER PEELFFVRM 
      RASLYRQTM DSILEDSPL DLSMSLLSR ALSGSPPSA 
      EDERKSVSG DSSAASSQG DLPEATVPT PRARPRERT 
      LLPCGVCNK AFDRPSLLK RHMRTHTGE KPHVCMVCG 
      KGFSTSSSL NTHRRIHSG EKPHQCPVC LKRFTASSN 
      LYYHRMTHI KDKPHKCNL CSKSFPTPG DLRSHMYVH 
      SGSWPFKCH ICSRGFSKH TNLKNHLFL HTGKPSATR 
      KG
    CDS sequence
    • >TC031254 RA
      atggagact tgtgtcctc attccgcag gaattcagt 
      ctttgcgtc cgaggtcct gcaccgcga cgcaccgcc 
      gaacctgaa gtttcagtt tggtcaaat accagcatc 
      gcgcaagga tcgctctgt tatcctttc caagggacg 
      atccggatt gataagctg gatgtctac ggacagctt 
      gatgatgat gatattcgg caccgtttc ggatgctac 
      gacgaaatc tccggaagt ggctcccgg agggtgcgc 
      cactgcaac tgggtccgg ttcgtgagg gtggccagt 
      tccttcaat ccggcggtt aatttcgtc gcaacgaag 
      gttaggggt gaaccggtt tacgaagcg gttaaaccg 
      atttcgccc gatacggaa ttactggtg tattattta 
      ccggaaagg ccggaagaa ctgtttttt gtgaggatg 
      agggcgtcg ctctaccgc caaacgatg gactcgatc 
      cttgaagac tctcccctc gacctctcc atgtccctc 
      ctctctcgg gccttgagt ggctctccc ccctctgct 
      gaagacgag cgaaaatcg gtttcagga gacagttca 
      gcggccagc tcgcagggc gacctccct gaagccacc 
      gtccccacc cccagagca cggccccgc gaacgcact 
      ttactccct tgtggagta tgcaacaaa gccttcgac 
      cgcccttcc ctcctcaaa agacacatg cggacgcac 
      accggcgag aaaccgcac gtgtgtatg gtgtgcgga 
      aaaggcttc agcacctcc tcctccctc aacacacat 
      cgacgcatc cacagcggg gaaaaaccc caccagtgt 
      cccgtgtgt cttaagcga ttcacagcc tcctccaat 
      ttatactac caccgaatg acgcacatc aaagataaa 
      ccgcacaaa tgcaatctc tgctccaag tcctttccg 
      actccgggc gatctcagg tcgcatatg tatgtccat 
      tccgggtcg tggcccttc aaatgccac atttgttca 
      cggggtttc tccaaacac acgaatctc aaaaatcat 
      ctattccta cacactggc aagccgtcc gcaactcgc 
      aagggctga
    mRNA sequence
    • >TC031254 RA
      agtgccact cctgcaagg ctgcggtca ctagatcac 
      cgcgaatcg cgcgttgtc tcttctcct gtcatgcac 
      tcttcatca tgtgttttt gactcttat tattgtcct 
      ctaactgat tgttgatcg atcgagagt ttgcaatgg 
      agacttgtg tcctcattc cgcaggaat tcagtcttt 
      gcgtccgag gtcctgcac cgcgacgca ccgccgaac 
      ctgaagttt cagtttggt caaatacca gcatcgcgc 
      aaggatcgc tctgttatc ctttccaag ggacgatcc 
      ggattgata agctggatg tctacggac agcttgatg 
      atgatgata ttcggcacc gtttcggat gctacgacg 
      aaatctccg gaagtggct cccggaggg tgcgccact 
      gcaactggg tccggttcg tgagggtgg ccagttcct 
      tcaatccgg cggttaatt tcgtcgcaa cgaaggtta 
      ggggtgaac cggtttacg aagcggtta aaccgattt 
      cgcccgata cggaattac tggtgtatt atttaccgg 
      aaaggccgg aagaactgt tttttgtga ggatgaggg 
      cgtcgctct accgccaaa cgatggact cgatccttg 
      aagactctc ccctcgacc tctccatgt ccctcctct 
      ctcgggcct tgagtggct ctcccccct ctgctgaag 
      acgagcgaa aatcggttt caggagaca gttcagcgg 
      ccagctcgc agggcgacc tccctgaag ccaccgtcc 
      ccaccccca gagcacggc cccgcgaac gcactttac 
      tcccttgtg gagtatgca acaaagcct tcgaccgcc 
      cttccctcc tcaaaagac acatgcgga cgcacaccg 
      gcgagaaac cgcacgtgt gtatggtgt gcggaaaag 
      gcttcagca cctcctcct ccctcaaca cacatcgac 
      gcatccaca gcggggaaa aaccccacc agtgtcccg 
      tgtgtctta agcgattca cagcctcct ccaatttat 
      actaccacc gaatgacgc acatcaaag ataaaccgc 
      acaaatgca atctctgct ccaagtcct ttccgactc 
      cgggcgatc tcaggtcgc atatgtatg tccattccg 
      ggtcgtggc ccttcaaat gccacattt gttcacggg 
      gtttctcca aacacacga atctcaaaa atcatctat 
      tcctacaca ctggcaagc cgtccgcaa ctcgcaagg 
      gctgaccaa attcaaaat tacaaattt gttgtaagt 
      gattttaat ttaatttaa tattgttgt ttgtataga 
      cttgtaaat actattttt acaccgaaa taaaactcg 
      tttttttcc