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Gene information
General information
TC011549 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid14: 2711989..2713950
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011549
    iBeetle screen iB_01864
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011549 RA
      MEQKLLDGL KDLGYCGPL LEKASFDSA LQEGAKNVE 
      FTKLVSFLT NELRALLNI DEEVNPISG PEDSVSFVM 
      EVSSFLKEL NCPYVALTQ GHISDRLES HQDKLLLLD 
      YLATELMAA RVLQEKKPD KKFELKLQE TEEAANMRK 
      ILMTLRFHK PPPNITIPQ LFQKVCPTV QSIVQKVGS 
      ELVGKPAFQ GTLSDKQWE TLNGVQKDL NNEYKIRRE 
      MLLTRLDVT IQSFQWSDK TKGKDELFE KVYTEKRKR 
      LKTEPEVEL ADLLAARDD IAIIEKTSN SSVRKNTKS 
      SLNKVIIGQ VPDRGGRTS EQAPPPPEM PSWQQRTPG 
      PSGGGRGRG GGNFSRNDN RQNYSRDSG SYGGSYSGN 
      QGGYDNYKS GGRGYDNYN QGRSYDSAG SYGGGYNNP 
      GYNNERQGS YGGGQGSYN DNKSSYDNR NFDSAGSYS 
      TSYEQNSKR PKTYDSFQQ NRTTYADQY VQEEQHNQQ 
      YHGRGNYRG RGRGHSNYN RGGRNHY
    CDS sequence
    • >TC011549 RA
      atggaacag aaactctta gacggactg aaggacctg 
      ggttattgt ggccccctt ctcgagaag gctagtttt 
      gattctgcc ctgcaggag ggagcaaaa aacgtggaa 
      ttcaccaag ctagtcagt tttttaaca aacgagcta 
      cgcgcattg cttaatatc gatgaggaa gtcaatcca 
      atatcaggc cctgaagat tcagtatcg tttgttatg 
      gaagttagt tcttttctg aaagaattg aactgcccc 
      tacgttgcc ttaacacag ggccatatt tcagaccgt 
      ctggaaagt caccaagac aaattatta cttttagac 
      tatttggct actgaactg atggccgca agagtacta 
      caagagaaa aagcccgat aaaaaattt gagctaaaa 
      ctgcaagag actgaagaa gctgccaat atgaggaag 
      atattaatg actctgagg tttcataaa ccgccccca 
      aacattact attccacag ttattccaa aaagtttgc 
      ccaactgtg caaagtatt gttcaaaaa gttggaagt 
      gaattagtt ggaaagcct gcgtttcaa ggaacactt 
      tctgataag cagtgggaa acactaaat ggggttcag 
      aaggatttg aacaatgaa tataaaatt agaagagag 
      atgttgcta acaaggctg gatgttacg attcagtct 
      tttcagtgg tctgataaa acgaaaggc aaagatgag 
      ttatttgaa aaagtttac accgaaaag cggaaacgc 
      ctgaaaacg gaaccggaa gttgaactt gctgattta 
      ttagccgca cgagacgac attgcaatt atcgaaaag 
      acaagtaac tcgtctgtg cgcaaaaac acaaaatcg 
      tcactaaat aaagtcatt attggacag gttcctgat 
      cgtggaggc cgaacctct gagcaagcg ccgccccct 
      ccggagatg ccctcttgg cagcagcgc acgccgggg 
      ccctcgggg ggcggtcga ggaagaggc ggcggtaac 
      ttttcccgt aacgacaac agacagaac tattcaaga 
      gattcgggg agttacggt ggaagttac tctggtaat 
      caaggtggt tatgataat tataaaagc ggcggtcgc 
      ggttacgac aattataac caggggcgc agttatgac 
      agtgctgga agttacggt ggtggttat aacaaccct 
      ggttataat aacgagaga caggggagt tacggcggc 
      ggccaaggc agttataac gataataaa agtagttac 
      gataatagg aattttgat agtgcaggt agttactcc 
      acgagctat gaacaaaat tcgaaaagg ccgaaaact 
      tacgattcc tttcaacaa aatagaacg acttatgct 
      gatcagtat gtgcaagag gaacagcat aatcagcag 
      tatcacggg agggggaat tacagaggg agaggcaga 
      ggacactcg aattacaac aggggaggc aggaatcac 
      tattaa
    mRNA sequence
    • >TC011549 RA
      attttcgca aattggtta gaaattcaa taaccggct 
      tcaatctct atattagtg caaaagtaa gacatagta 
      ttaattaat acaatggaa cagaaactc ttagacgga 
      ctgaaggac ctgggttat tgtggcccc cttctcgag 
      aaggctagt tttgattct gccctgcag gagggagca 
      aaaaacgtg gaattcacc aagctagtc agtttttta 
      acaaacgag ctacgcgca ttgcttaat atcgatgag 
      gaagtcaat ccaatatca ggccctgaa gattcagta 
      tcgtttgtt atggaagtt agttctttt ctgaaagaa 
      ttgaactgc ccctacgtt gccttaaca cagggccat 
      atttcagac cgtctggaa agtcaccaa gacaaatta 
      ttactttta gactatttg gctactgaa ctgatggcc 
      gcaagagta ctacaagag aaaaagccc gataaaaaa 
      tttgagcta aaactgcaa gagactgaa gaagctgcc 
      aatatgagg aagatatta atgactctg aggtttcat 
      aaaccgccc ccaaacatt actattcca cagttattc 
      caaaaagtt tgcccaact gtgcaaagt attgttcaa 
      aaagttgga agtgaatta gttggaaag cctgcgttt 
      caaggaaca ctttctgat aagcagtgg gaaacacta 
      aatggggtt cagaaggat ttgaacaat gaatataaa 
      attagaaga gagatgttg ctaacaagg ctggatgtt 
      acgattcag tcttttcag tggtctgat aaaacgaaa 
      ggcaaagat gagttattt gaaaaagtt tacaccgaa 
      aagcggaaa cgcctgaaa acggaaccg gaagttgaa 
      cttgctgat ttattagcc gcacgagac gacattgca 
      attatcgaa aagacaagt aactcgtct gtgcgcaaa 
      aacacaaaa tcgtcacta aataaagtc attattgga 
      caggttcct gatcgtgga ggccgaacc tctgagcaa 
      gcgccgccc cctccggag atgccctct tggcagcag 
      cgcacgccg gggccctcg gggggcggt cgaggaaga 
      ggcggcggt aacttttcc cgtaacgac aacagacag 
      aactattca agagattcg gggagttac ggtggaagt 
      tactctggt aatcaaggt ggttatgat aattataaa 
      agcggcggt cgcggttac gacaattat aaccagggg 
      cgcagttat gacagtgct ggaagttac ggtggtggt 
      tataacaac cctggttat aataacgag agacagggg 
      agttacggc ggcggccaa ggcagttat aacgataat 
      aaaagtagt tacgataat aggaatttt gatagtgca 
      ggtagttac tccacgagc tatgaacaa aattcgaaa 
      aggccgaaa acttacgat tcctttcaa caaaataga 
      acgacttat gctgatcag tatgtgcaa gaggaacag 
      cataatcag cagtatcac gggaggggg aattacaga 
      gggagaggc agaggacac tcgaattac aacagggga 
      ggcaggaat cactattaa aaattgtgc tgtatcatt 
      atttgcagt tatttgaaa ctagcacta acacttgaa 
      agattgcta atgtattta aatatttaa taaaagtac 
      tgaatcgat