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Gene information
General information
TC011529 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3658961..3661119
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011529
    iBeetle screen iB_01862

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 11.11% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 11.11% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011529 RA
      MFVKGVVLL LFLSTQFLC YEQFVIPDV TLEAYAPKG 
      FRASIPALP GIQMFAFHM NVNKKISQV DPGDYRQDY 
      TSPDGNVWS YFNSDLSLN IGDTVNYWI FVQHEKLGY 
      RKDNVEWTV TELLQLPNG TCEPPLTVV SGQTQVCKG 
      QVVFEENFR GDKINENKW TLEQYIPTY TSVDSEFVS 
      YQKKQCYIF GEKLFIKPN PAQNDDDVN GELDFRGKC 
      TRATERACH IVREIYLIV PPVASGRIV SKFKFRYGT 
      VEIKAKLPA GDWIYPQIY LEKVSDPKD KIWISYARG 
      NNQLLLKNK QDLGGNLLF GGLVIDPEE PGRSQFLKT 
      KRSNTPFSR EMHVYTVIW TKDKLSLLL DGTQYGEID 
      KEALSQFNF SDDDLVQIV LGVGVGGNN DFPDSVQSG 
      NHKKPWVNK DPKEVKFFF NARSEWLGT WKGDNTALQ 
      VDYVKVTAL
    CDS sequence
    • >TC011529 RA
      atgttcgtt aaaggagta gtactgttg ctatttctt 
      tcgacacaa tttctttgt tatgaacag tttgtgatc 
      ccagatgtt acgctagaa gcctatgct cctaaagga 
      ttcagggca tctatacct gctctgcca ggtattcag 
      atgttcgca tttcacatg aatgtaaac aaaaagata 
      tcacaagtt gatcccggc gattacaga caggactac 
      acttcgcct gacggtaat gtgtggtct tatttcaat 
      agtgatttg tccctcaac atcggagac accgtcaac 
      tattggatt ttcgtccaa catgaaaaa ttggggtac 
      cgaaaagac aacgtagaa tggactgtt acagaactt 
      ctccaatta ccaaatggt acttgtgaa ccgccttta 
      actgtggtt agtggacaa acccaggtt tgcaaaggc 
      caggttgtg ttcgaagag aattttcga ggtgataaa 
      atcaatgag aataaatgg actctcgaa cagtacata 
      ccaacatac accagtgtg gattcggaa tttgtttcc 
      taccagaaa aaacaatgt tacattttt ggagagaaa 
      ttatttata aaaccaaac cctgcgcaa aacgacgac 
      gacgtgaac ggtgaatta gacttccgt ggaaagtgt 
      acgcgtgct accgaaagg gcgtgccac attgtacga 
      gagatctac ttaatagtt ccaccagta gcttcgggt 
      agaatagtt tcaaaattt aaatttcga tatggtaca 
      gtcgaaatt aaagccaag ttgccagca ggagactgg 
      atttatccg caaatttat ttggaaaaa gttagcgac 
      cctaaagat aaaatatgg atcagttat gccagaggc 
      aataatcaa ctgttatta aaaaataag caagatctt 
      ggtggtaac ttacttttt ggtggactg gtaattgat 
      ccagaggag cctggaaga agccagttt ttaaaaact 
      aaacgcagc aacactccg ttttcgagg gaaatgcat 
      gtctatacg gtcatttgg accaaagac aaattgtcg 
      ttactactc gatggtact cagtatggt gaaatagac 
      aaggaagcc ctcagccaa tttaacttt tcagacgat 
      gatttggtc caaatagtt ttgggagtt ggcgttggt 
      ggaaataat gacttcccc gacagtgtc cagtcgggg 
      aaccacaaa aaaccatgg gttaataaa gatcccaaa 
      gaagtgaaa ttcttcttt aacgcaagg tctgagtgg 
      cttgggacc tggaagggt gataataca gctttacaa 
      gtcgattac gtcaaagtt acggcttta taa
    mRNA sequence
    • >TC011529 RA
      catatttct attgtatca attaatttg caaatgtta 
      ttgatctat tttcattat gataataaa gatttgatg 
      aaaccagtt tgttcgcct gcaaggtca gttcaagac 
      atggagaat cccagatta cagttatct taatgtaat 
      tcccagttc attagagaa ataacaatc cttagtggc 
      agaatgttc gttaaagga gtagtactg ttgctattt 
      ctttcgaca caatttctt tgttatgaa cagtttgtg 
      atcccagat gttacgcta gaagcctat gctcctaaa 
      ggattcagg gcatctata cctgctctg ccaggtatt 
      cagatgttc gcatttcac atgaatgta aacaaaaag 
      atatcacaa gttgatccc ggcgattac agacaggac 
      tacacttcg cctgacggt aatgtgtgg tcttatttc 
      aatagtgat ttgtccctc aacatcgga gacaccgtc 
      aactattgg attttcgtc caacatgaa aaattgggg 
      taccgaaaa gacaacgta gaatggact gttacagaa 
      cttctccaa ttaccaaat ggtacttgt gaaccgcct 
      ttaactgtg gttagtgga caaacccag gtttgcaaa 
      ggccaggtt gtgttcgaa gagaatttt cgaggtgat 
      aaaatcaat gagaataaa tggactctc gaacagtac 
      ataccaaca tacaccagt gtggattcg gaatttgtt 
      tcctaccag aaaaaacaa tgttacatt tttggagag 
      aaattattt ataaaacca aaccctgcg caaaacgac 
      gacgacgtg aacggtgaa ttagacttc cgtggaaag 
      tgtacgcgt gctaccgaa agggcgtgc cacattgta 
      cgagagatc tacttaata gttccacca gtagcttcg 
      ggtagaata gtttcaaaa tttaaattt cgatatggt 
      acagtcgaa attaaagcc aagttgcca gcaggagac 
      tggatttat ccgcaaatt tatttggaa aaagttagc 
      gaccctaaa gataaaata tggatcagt tatgccaga 
      ggcaataat caactgtta ttaaaaaat aagcaagat 
      cttggtggt aacttactt tttggtgga ctggtaatt 
      gatccagag gagcctgga agaagccag tttttaaaa 
      actaaacgc agcaacact ccgttttcg agggaaatg 
      catgtctat acggtcatt tggaccaaa gacaaattg 
      tcgttacta ctcgatggt actcagtat ggtgaaata 
      gacaaggaa gccctcagc caatttaac ttttcagac 
      gatgatttg gtccaaata gttttggga gttggcgtt 
      ggtggaaat aatgacttc cccgacagt gtccagtcg 
      gggaaccac aaaaaacca tgggttaat aaagatccc 
      aaagaagtg aaattcttc tttaacgca aggtctgag 
      tggcttggg acctggaag ggtgataat acagcttta 
      caagtcgat tacgtcaaa gttacggct ttataagat 
      attaaaatc gtgtaagtt acttaacga aacacttgt 
      gccatatca atttattgt ccgaataaa aacattaaa 
      atcat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY