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Gene information
General information
TC011527 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3697422..3701280
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011527
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01861

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber complete not present - Number of animals showing the phenotype: 4
    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
    ovary size decreased - Number of animals showing the phenotype: 4
    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 4
    tropharium potentially size decreased - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Comment: all dead animals partially kannibalized

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011527 RA
      MAAANLRLP PLPTVRDLI KLYKLRALR QLSQNFLLD 
      ERITDKIVK AAGNIRNHY VCEVGPGPG SITRSIIRK 
      CPKKLIVVE KDPRFVPIL ELLQEASQS HVKMGIKIQ 
      DIRDFNFEE GFAGAPARE WTDYPPPIH LIGNLPFSV 
      STNLIIRWL HAISEQRSA WSFGRSTMT LTFQKEVAE 
      RIVAPVTHE QRCRLSVMC QFWCDVEHK FTIPGRAFV 
      PKPDVDVGV VTLVPKKHP LVKLPFKTV EKILRTVFN 
      MRQKWSIKG GGRLFPEDK RDELAVKLF ALADVDSRI 
      RPFEISNEE FARICYAYK VICEEDPEI ESYDFRAPK 
      KLNVVAG
    CDS sequence
    • >TC011527 RA
      atggcagcg gccaatctt aggttaccc cccttgccc 
      acagtgagg gacttaatt aagttgtat aagttaagg 
      gcgttacgt cagctatcg caaaacttt cttttggac 
      gaaagaatc accgacaaa atcgttaaa gcggctgga 
      aatattcgg aatcattat gtttgtgaa gtgggccct 
      gggcccggc agtataacc cggtccatc atcaggaag 
      tgccccaag aagctcatc gtggtggaa aaggacccc 
      cgatttgtg cccatcttg gagttgctt caagaagcg 
      agtcagagt cacgtcaaa atggggatt aaaattcag 
      gatatccgg gattttaat ttcgaggag ggttttgcg 
      ggcgctcct gcacgcgag tggacggac tacccgccc 
      cccattcac ctcattggc aatctgccc ttcagcgtt 
      tcaacgaat ttgattatc cggtggtta catgcgatt 
      tcggagcaa aggtcggct tggagtttt gggcgctct 
      accatgacc ttgacgttc cagaaggaa gttgctgaa 
      aggattgtc gctccagtg actcatgaa cagagatgc 
      agactctct gtaatgtgc cagttctgg tgcgatgtg 
      gaacacaaa ttcactatt ccagggagg gcttttgtc 
      cccaaaccg gatgttgat gtcggggtt gtaacactg 
      gttcctaag aagcatccg ttggtgaag ttaccgttt 
      aaaacggtt gagaaaatt ctcaggact gtcttcaat 
      atgcggcaa aagtggtcc atcaagggc gggggcagg 
      ctgttccca gaggataag cgggacgaa ctagctgtt 
      aaactgttt gctctggct gatgtcgac tcacgaatt 
      cgccctttc gaaatatca aacgaagaa ttcgcccgc 
      atttgctac gcctacaaa gtaatctgt gaggaagac 
      cccgaaatt gaaagctac gactttaga gcccctaaa 
      aagttgaat gttgttgct gggtag
    mRNA sequence
    • >TC011527 RA
      aaagacatt aattaactt tcgaggtta tgtccgcac 
      ttttttgca caaatcccc aaaacagtt aatatttac 
      gatgtcaga gagtgatcg catgcccaa aattgaccg 
      caccaaact gaaacaaac cgatatcga cttcataaa 
      attcgtgga aaagcagaa tttggaccg tgtccgaaa 
      actgcaaac ccttcgccg caaaaacct gatcactgt 
      tagcctcct tggaactgc agtcttggg catttacgg 
      ctattccat ttgggcagt gcgacaaga aaacttcct 
      cgacgattt tgaaatccc aaccactgt aacagaatc 
      agcaaagta gtaatggca gcggccaat cttaggtta 
      ccccccttg cccacagtg agggactta attaagttg 
      tataagtta agggcgtta cgtcagcta tcgcaaaac 
      tttcttttg gacgaaaga atcaccgac aaaatcgtt 
      aaagcggct ggaaatatt cggaatcat tatgtttgt 
      gaagtgggc cctgggccc ggcagtata acccggtcc 
      atcatcagg aagtgcccc aagaagctc atcgtggtg 
      gaaaaggac ccccgattt gtgcccatc ttggagttg 
      cttcaagaa gcgagtcag agtcacgtc aaaatgggg 
      attaaaatt caggatatc cgggatttt aatttcgag 
      gagggtttt gcgggcgct cctgcacgc gagtggacg 
      gactacccg ccccccatt cacctcatt ggcaatctg 
      cccttcagc gtttcaacg aatttgatt atccggtgg 
      ttacatgcg atttcggag caaaggtcg gcttggagt 
      tttgggcgc tctaccatg accttgacg ttccagaag 
      gaagttgct gaaaggatt gtcgctcca gtgactcat 
      gaacagaga tgcagactc tctgtaatg tgccagttc 
      tggtgcgat gtggaacac aaattcact attccaggg 
      agggctttt gtccccaaa ccggatgtt gatgtcggg 
      gttgtaaca ctggttcct aagaagcat ccgttggtg 
      aagttaccg tttaaaacg gttgagaaa attctcagg 
      actgtcttc aatatgcgg caaaagtgg tccatcaag 
      ggcgggggc aggctgttc ccagaggat aagcgggac 
      gaactagct gttaaactg tttgctctg gctgatgtc 
      gactcacga attcgccct ttcgaaata tcaaacgaa 
      gaattcgcc cgcatttgc tacgcctac aaagtaatc 
      tgtgaggaa gaccccgaa attgaaagc tacgacttt 
      agagcccct aaaaagttg aatgttgtt gctgggtag 
      tctggtttg ttagtcatt tgtttagtg ttgattaat 
      aaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY