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Gene information
General information
TC011519 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3769416..3771793
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011519
    iBeetle screen iB_01859

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    remarks: later than schedule,
    Pupal morphology
    remarks: later than schedule,
    Fertility
    tropharium partiallystructure irregular - Number of animals showing the phenotype: 3

    previtellogenic egg chamber partiallynumber decreased - Number of animals showing the phenotype: 4

    vitellogenic egg chamber completenot present - Number of animals showing the phenotype: 4

    vitellarium completethinner - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection



    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011519 RA
      MASVLEQFV TTVRTLSSH GNYKVLCEY LSKSSDILL 
      KNTQHLDNV LETLDLQQH SLGYLVVLC AKFNSTVPA 
      NTPDNRFAQ VQEFIHGCN GEQIRLAPD TFAELCHSL 
      TNYLVEQKQ PMKGILLLR KAISKLRFF DSQLTSIHA 
      DLCQLCLLA KCFKPALEI LDTDITGIC QEIVHNPNG 
      AQFEAKYFL LYYYYGGMI YLAVRNLDR ALYFFEVAI 
      TTPAHAVSH IMLEAYKKY IIVSLLLHG KIQPVPKYA 
      SQVVTRFIK PLSQPYNDL ANSFTSNNT ADLNAVLNK 
      HREAFTRDH NLGLVKQVS SVLYKKNIQ RLTKTFLTL 
      SLSDVASRV GLPGPSDAE RYILHMIED QQIYATINQ 
      KDGMVVFRD EPEKYGGPD VLKSLEAQL AICMELDKQ 
      ILAMDEDIQ VNPVYVKKS SGVQDEEQP SKSTYAM
    CDS sequence
    • >TC011519 RA
      atggcatcc gttttagaa cagtttgtg acgactgtt 
      aggaccctc tcatcccat ggaaactat aaagttctt 
      tgtgaatat ttaagtaaa tcgtcggat atcctcctg 
      aaaaacacg cagcacttg gacaacgtg ctcgagacg 
      ctcgatctg cagcagcac tcgctcggc tatttggtg 
      gtgttatgc gctaaattt aattcaaca gtcccagct 
      aatacgcca gacaatcgg tttgcacaa gtgcaagag 
      tttatccat ggctgtaac ggagagcag atcagactg 
      gcccccgac acttttgct gaattatgc cactctcta 
      acaaattat ctagttgag cagaagcaa cccatgaaa 
      gggattctc ctcttacga aaggccatt tcgaagttg 
      cggttcttc gattcgcag ttaacttcg atccatgct 
      gatctgtgc cagttgtgc ttattggca aagtgcttt 
      aagcctgct ctagaaatt ttagatact gatattact 
      ggaatttgc caagagatt gttcataat cctaatggg 
      gcccaattt gaagcaaaa tatttttta ttgtattac 
      tattatggc ggaatgatt tatttagct gtgagaaat 
      ttggatcgt gctttgtat ttctttgag gtagctata 
      acaacccca gcacatgct gtttctcat attatgctc 
      gaggcatac aagaagtac attatagtt tcactttta 
      ttacatgga aaaatacaa ccagtgcct aaatatgct 
      tcacaagtg gttactcgg tttatcaaa ccattatct 
      cagccttat aatgattta gcaaattct ttcacttca 
      aacaacaca gcggatttg aatgctgta cttaacaaa 
      catagagag gcattcacc agggatcac aatttaggt 
      cttgtaaaa caggtatcc tcagtgctt tacaagaaa 
      aacattcaa aggctgacg aaaaccttc ttaacgtta 
      agtttgtca gatgtggcg agccgagtc ggattgcct 
      ggcccttcg gatgccgag cgctacatt ttacacatg 
      atcgaagat cagcaaatc tacgccacg ataaaccaa 
      aaagacgga atggttgtg tttagagac gagcctgag 
      aaatacggg ggcccagac gtgctgaaa agcctagaa 
      gctcaacta gcaatttgt atggaactt gacaaacaa 
      atccttgca atggacgaa gacatccaa gtgaatccc 
      gtctacgtg aagaagtcg agcggtgta caagacgag 
      gagcagccc agcaaaagt acatacgcg atgtaa
    mRNA sequence
    • >TC011519 RA
      tctgtcatc acaccggtt gtcaattca gatcctagt 
      ataaaaata gtaataatt tattcagca aaacgtaat 
      tttcccaca tccgaatga aatggcatc cgttttaga 
      acagtttgt gacgactgt taggaccct ctcatccca 
      tggaaacta taaagttct ttgtgaata tttaagtaa 
      atcgtcgga tatcctcct gaaaaacac gcagcactt 
      ggacaacgt gctcgagac gctcgatct gcagcagca 
      ctcgctcgg ctatttggt ggtgttatg cgctaaatt 
      taattcaac agtcccagc taatacgcc agacaatcg 
      gtttgcaca agtgcaaga gtttatcca tggctgtaa 
      cggagagca gatcagact ggcccccga cacttttgc 
      tgaattatg ccactctct aacaaatta tctagttga 
      gcagaagca acccatgaa agggattct cctcttacg 
      aaaggccat ttcgaagtt gcggttctt cgattcgca 
      gttaacttc gatccatgc tgatctgtg ccagttgtg 
      cttattggc aaagtgctt taagcctgc tctagaaat 
      tttagatac tgatattac tggaatttg ccaagagat 
      tgttcataa tcctaatgg ggcccaatt tgaagcaaa 
      atatttttt attgtatta ctattatgg cggaatgat 
      ttatttagc tgtgagaaa tttggatcg tgctttgta 
      tttctttga ggtagctat aacaacccc agcacatgc 
      tgtttctca tattatgct cgaggcata caagaagta 
      cattatagt ttcactttt attacatgg aaaaataca 
      accagtgcc taaatatgc ttcacaagt ggttactcg 
      gtttatcaa accattatc tcagcctta taatgattt 
      agcaaattc tttcacttc aaacaacac agcggattt 
      gaatgctgt acttaacaa acatagaga ggcattcac 
      cagggatca caatttagg tcttgtaaa acaggtatc 
      ctcagtgct ttacaagaa aaacattca aaggctgac 
      gaaaacctt cttaacgtt aagtttgtc agatgtggc 
      gagccgagt cggattgcc tggcccttc ggatgccga 
      gcgctacat tttacacat gatcgaaga tcagcaaat 
      ctacgccac gataaacca aaaagacgg aatggttgt 
      gtttagaga cgagcctga gaaatacgg gggcccaga 
      cgtgctgaa aagcctaga agctcaact agcaatttg 
      tatggaact tgacaaaca aatccttgc aatggacga 
      agacatcca agtgaatcc cgtctacgt gaagaagtc 
      gagcggtgt acaagacga ggagcagcc cagcaaaag 
      tacatacgc gatgtaaaa atttcagtt gctcagtgt 
      tatcagttt tcattttgt acacttgaa atatttaag 
      ctttttcat gaagaaagg gaaacgtca gctcattaa 
      cttgtaatc gaaattata acattataa acactgtgt 
      gtttttgta gtttaatac catttctca gagacgtgt 
      acattattt attttgtgt ttctttaag taataacaa 
      ttcgagatt tacattgta ctgaggtta tttacaata 
      aaatactga t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY