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Gene information
General information
TC011518 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid14: 2707278..2708867
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011518
    iBeetle screen iB_01858
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011518 RA
      MFSCTIPKL RIIFPTARS LSSLASPSQ IEPRQLAFP 
      PKYQQPRQV WLENFDTIE EKKLAILEL HPEVFAANP 
      RVDIIHDNV RWQSLYRYV SYAHTKSRF EVRGGGRKP 
      WPQKGLGRA RHGSIRSPL FRGGGVIHG PKSPNPHFY 
      MLPFYTRIH GLTATLSIK LAQDDLHVI TDLEIPTDD 
      PNFVEELVK SRKWGPSVL FVDESDIMP RNITAATDS 
      VKHFNLMPA YGLNVYSML KHDTLVLTR AALDLIESK 
      LLTHLHKNN SVPNTQKYK LNQV
    CDS sequence
    • >TC011518 RA
      atgttttct tgcacaatt cccaaatta cgaataatc 
      tttccaacc gcgcgttcg ctctcctcg cttgctagc 
      ccttcccag atcgagcct cgccaactg gctttcccc 
      cccaaatac caacaaccc cgtcaagtt tggttagaa 
      aactttgac acaatcgaa gaaaagaaa ctcgcaata 
      ctagaacta catccagag gttttcgca gccaacccc 
      cgagtcgac ataatccac gataacgtg cggtggcaa 
      tctctgtat cgctacgtg agttacgcg cacacaaaa 
      tcgcggttt gaagtgcga ggaggaggg cgtaagcct 
      tggccccag aagggtctc gggagggcg cggcatggg 
      tcgatacgc agccccctg tttaggggc ggcggggtg 
      atccacggc cctaaatcg cccaaccca cacttttac 
      atgttgccg ttttatacc aggattcat gggttgacc 
      gcaacgttg tcgataaag ttggctcag gatgattta 
      cacgtgatc actgatttg gagataccg actgatgat 
      ccaaacttt gtggaggaa ttggtcaag agcaggaag 
      tgggggcct tcggtcttg tttgtggac gagtccgat 
      attatgccg agaaatatc actgcagca actgattcc 
      gttaaacat tttaatttg atgccagct tatgggctt 
      aatgtttat tcaatgctt aagcatgat acgttggtg 
      ttaacgagg gctgcactt gatctgatc gaatcaaaa 
      ctcttaacg catctgcac aagaacaac tctgtgcct 
      aatacacag aaatacaag ttaaatcaa gtgtaa
    mRNA sequence
    • >TC011518 RA
      tgacgaagc tgtcaacta gtttcaggt gcacgtgtt 
      tgcagccca aaaaatacc taaaatatg gataaacca 
      gtcgttttg gcacgtgtt attaaggtc ctaggccgt 
      actgggtcc cagggacag tgtacacaa gttaaggtt 
      gagttcatc ggggaacaa aaccgacaa attatccgc 
      aatgttaag ggcccagtg agagaaggg gacattctt 
      accctcctg gaatccgaa cgtgaagct aggagatta 
      agataattt gcttaagga tatccatgg tttattcgt 
      gggaaatga ataaaaata gtttataat acttcaatt 
      ctgttttct tttataata aacaatttc cgacgaatt 
      atcggtttt ttattccct taatactgt cctttttca 
      attacggcg acatttgag tttcgaaaa ttcgtaaaa 
      taccgtttt ttttcgatt aaataagtt agaaaacga 
      caactgtat tgtgtgttg cataccata ccgggcgtt 
      tttgaggtt gtgtccgtc taatttagg ttaagttag 
      tggacttga aacagctaa aatgttttc ttgcacaat 
      tcccaaatt acgaataat ctttccaac cgcgcgttc 
      gctctcctc gcttgctag cccttccca gatcgagcc 
      tcgccaact ggctttccc ccccaaata ccaacaacc 
      ccgtcaagt ttggttaga aaactttga cacaatcga 
      agaaaagaa actcgcaat actagaact acatccaga 
      ggttttcgc agccaaccc ccgagtcga cataatcca 
      cgataacgt gcggtggca atctctgta tcgctacgt 
      gagttacgc gcacacaaa atcgcggtt tgaagtgcg 
      aggaggagg gcgtaagcc ttggcccca gaagggtct 
      cgggagggc gcggcatgg gtcgatacg cagccccct 
      gtttagggg cggcggggt gatccacgg ccctaaatc 
      gcccaaccc acactttta catgttgcc gttttatac 
      caggattca tgggttgac cgcaacgtt gtcgataaa 
      gttggctca ggatgattt acacgtgat cactgattt 
      ggagatacc gactgatga tccaaactt tgtggagga 
      attggtcaa gagcaggaa gtgggggcc ttcggtctt 
      gtttgtgga cgagtccga tattatgcc gagaaatat 
      cactgcagc aactgattc cgttaaaca ttttaattt 
      gatgccagc ttatgggct taatgttta ttcaatgct 
      taagcatga tacgttggt gttaacgag ggctgcact 
      tgatctgat cgaatcaaa actcttaac gcatctgca 
      caagaacaa ctctgtgcc taatacaca gaaatacaa 
      gttaaatca agtgtaatt tttaataag aaataaatt 
      gtttagtca aat