Status
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Gene information
General information
TC031473
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011512
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid14: 2748590..2756317
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031473
    iBeetle screen iB_01857
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031473 RA
      MSSAEIAEG SVDPPTKKR KLDSCPIST DMANNGASG 
      SSQQDIDEG LYSRQLYVL GHDAMRRMA SSDVLVSGL 
      GGLGVEVAK NVILGGVKS VTLHDEAVC SVADLSSQF 
      YFTEADVGK NRAEVCCKQ LAELNTYVP TKAYTGPLT 
      PDFIRKFRV VVLTNSCLA EQMQISEIT HASNIALIV 
      ADTRGLFAQ VFCDFGDAF TVVDTNGES PVSAMIADI 
      STDKEGIVT CIDDTRHGM EDGDHVTFS EVQGMTELN 
      NCKPIKIKV LGPYTFSIG DTTNFSKYE RGGIATQVK 
      MPKTLSFKS LKDSLKSPE FLMTDFAKF DHPQQLHVA 
      FQALHKYVE KHGRVPKPW NNEDASEFL SIAKSLAVD 
      GGNDTEVNT NLLETFAKV CAGDLNPIN ATIGGIVAQ 
      EVMKACSGK FHPIYQWLY FDAIECLPT EAGEITEES 
      AAPKGTRYD GQIAVFGSE FQKKLGGLK YFVVGAGAI 
      GCELLKNFA MMGIGGEGG QITVTDMDL IEKSNLNRQ 
      FLFRPHDVQ RPKSGTAAK VIKKMNPSI NIVAHENRV 
      GPESENMYD DTFFESLDG VANALDNVD ARIYMDRRC 
      VYYRKPLLE SGTLGTKGN TQVVVPFLT ESYSSSQDP 
      PEKSIPICT LKNFPNAIE HTLQWARDN FEGLFRQSA 
      ENASQYLTD PEFLERTIK LPGVQPIEV LESVKAALV 
      DDRPKSFED CVVWARHHW EEQYSNQIK QLLFNFPPD 
      QKTSTGQLF WSGPKRCPE PLTFDVNNT LHLDYVFAA 
      ANLKAEVYG IPQNRDRAY IAEVVQKIH VPEFVPKSG 
      VKIAVTDSQ MAMTNGSSV DLDRVGQIR EELPSVAEL 
      GNLCLTPLD FEKDDDTNL HMDFIVAAS NLRAANYKI 
      PPADRHKSK LIAGKIIPA IATTTSVVA GLVCLELYK 
      LTRSLKSLT PFKNGFVNL ALPFFGFSE PIAAPKNEY 
      CGKEWTLWD RFEVDGEMT LSEFLEYFK EKHGLEITM 
      LSQGVCMLY SFFMAKAKA QERLGLPMS EIVKRVSKK 
      KLEPHVKAL VFELCCNDA DGNDVEVPY VKYNLP
    CDS sequence
    • >TC031473 RA
      atgtctagt gctgaaatc gccgaaggc tccgtcgat 
      cccccaact aagaagcgg aagcttgac tcgtgcccg 
      atttcaacc gacatggcg aacaacggc gcttccgga 
      tcgtctcag caggacatc gacgagggt ctgtactcg 
      cgccagctc tacgtcctg gggcacgac gccatgcgg 
      cgcatggcc tccagcgac gtgctggtc tcaggcctg 
      ggcgggctc ggcgtcgag gtggccaag aacgtcatc 
      ctcggcggg gtcaagtcc gtcacgttg catgacgaa 
      gccgtttgc agcgtcgcc gacctctcg tcgcagttc 
      tacttcacc gaagccgac gtgggcaag aacagggcc 
      gaggtctgc tgcaagcag ctggcggag ctcaacacc 
      tacgtcccc acgaaggcc tacacgggc cccctgacg 
      ccggacttc atccgcaag ttccgagtc gttgtcctg 
      accaatagt tgtctcgct gagcagatg caaatcagc 
      gaaatcaca catgccagt aacattgcg ctcattgtg 
      gccgatacg agggggctc tttgcccaa gtgttctgt 
      gatttcggg gatgctttc acggtcgtt gacaccaac 
      ggggagtcg ccggtctcg gcgatgatc gctgatatt 
      tccaccgat aaagaaggc attgtgacc tgcattgac 
      gacacccgc catggcatg gaagacggc gaccacgtc 
      acattttcc gaagtccaa ggaatgacc gaactgaac 
      aattgcaag ccgattaaa atcaaagtg ttaggccct 
      tacacgttc agcatcggc gatacgacc aacttttcg 
      aaatacgag cgtggcggt attgccact caagtgaaa 
      atgcccaaa accttgtcg tttaaatca ctcaaagac 
      tcgctaaaa tcgcccgaa tttttgatg accgatttc 
      gcgaaattc gatcatcca cagcagctc cacgtggct 
      tttcaagcg ttgcacaaa tacgtcgaa aagcacggc 
      cgtgtacca aaaccgtgg aacaacgaa gacgcatcc 
      gaatttctg tccattgct aaatctttg gccgtggac 
      ggcggaaac gataccgaa gtgaacact aacttgtta 
      gaaacgttt gcgaaagtg tgcgcgggt gatttgaat 
      ccgattaat gcgactatc ggtggtatc gtggcgcag 
      gaagttatg aaagcttgt tcggggaaa tttcacccg 
      atttatcag tggttgtat ttcgatgcg attgaatgt 
      ttgcccacg gaagctggg gaaattacc gaagagtcg 
      gcggcaccg aaaggcaca cgctatgat ggacaaatt 
      gcggtcttc ggaagcgaa ttccagaag aaattaggc 
      ggtttgaaa tatttcgta gtaggtgct ggagcgatc 
      ggttgcgaa ttacttaaa aatttcgct atgatggga 
      attggtggt gaaggtgga caaatcacc gtcactgat 
      atggatctt attgaaaaa tctaatctc aatcgacag 
      tttttgttc agaccacat gatgttcag cgtcccaaa 
      tctggaacg gcagcaaag gtgatcaag aaaatgaac 
      ccgagcata aacatagtc gcccatgaa aatcgcgtg 
      ggccccgag tctgaaaac atgtacgat gacactttc 
      ttcgagtcg ttagacggc gtagctaac gctttagat 
      aacgttgac gctcgtatc tacatggac cgtcgatgt 
      gtctattac cgaaaacct ctactagaa tccggaacc 
      ttgggtaca aaaggcaac acccaggtc gtagtgcct 
      ttcctcacc gaatcctac agttcgtca caggaccct 
      cccgagaag agcatcccg atttgtacc cttaaaaat 
      ttccccaac gccatcgaa cacactctc caatgggct 
      cgtgataat ttcgaaggc cttttcaga cagagtgct 
      gaaaacgca tcgcaatat ctcaccgat cctgaattt 
      ttggaacgt actattaaa ttaccagga gttcaaccg 
      attgaagtg ctggaatcg gtcaaggca gctttagtt 
      gatgatagg ccaaagagt tttgaagac tgtgtagtg 
      tgggcccgc catcattgg gaggaacaa tattcaaat 
      cagatcaaa caactgctt tttaatttc ccgccggat 
      caaaaaacc agcactgga cagttgttc tggtcggga 
      ccgaagagg tgtcccgaa ccactcaca ttcgatgtg 
      aacaatacc ttgcatttg gattatgtt tttgcagcg 
      gctaatttg aaagccgag gtttatgga ataccgcag 
      aatagggat cgggcttac atcgccgaa gtggtgcaa 
      aaaattcac gtaccagag ttcgtcccg aaatctggc 
      gtcaagatc gccgtcacc gattcgcag atggcaatg 
      acgaacggt tcaagcgtc gacttggac cgcgttggc 
      caaatcaga gaggaatta ccttcggtc gccgaactg 
      ggcaatctt tgcctcact ccacttgat ttcgaaaag 
      gacgacgac acaaacctc cacatggat ttcatagta 
      gcggcttcg aatctccga gccgccaac tacaaaatc 
      cctccagcc gatcgtcac aaatccaag ctgatcgcc 
      ggcaaaatc atcccagct atagcgaca acaacctca 
      gtcgtcgcc ggcctcgtc tgtctcgag ctttacaaa 
      ttaacgaga agtttgaaa tcgttgacg ccgttcaag 
      aacggtttc gtcaacttg gctctgccg ttcttcggg 
      ttttcggag cccattgcc gcccccaag aacgagtat 
      tgcgggaag gagtggacg ttgtgggac cggtttgag 
      gtggatggg gagatgact ctgagcgag tttttggag 
      tactttaaa gagaagcac ggcttggag atcacgatg 
      ttgtcgcag ggcgtgtgc atgctgtac tcgttcttt 
      atggccaaa gcgaaggcg caggaacgc ctggggttg 
      cccatgtcc gagattgtg aagcgagtg tcgaagaag 
      aagctcgaa ccgcacgtc aaggcgcta gtgtttgaa 
      ctgtgttgt aacgacgct gacggaaac gatgtcgaa 
      gttccgtat gttaagtat aatctacct tga
    mRNA sequence
    • >TC031473 RA
      tgtcctttc ttacaggaa ttttcgtat ggtttgtgg 
      gaaaattat tttcgagta aatcgcttc acttgggtc 
      gtttcggtg cccccattg ctctgctta acaatcagt 
      gtgaatctt tcgcgacaa tttccaatt tagcgcgta 
      agtttgcag aattgtggg agtgcgtga atcacgcat 
      tccggcatg cgcattcgg cccgtggtc tctttgttc 
      gttcgttgt gcgccttca gccagcgtt gtgtctaaa 
      tccaagtga aaaaaccgg ccaattgac attgctata 
      tggttatca gcgcgcaaa ttcctcgat tgacccgca 
      actaatcaa actgtgtcc tttcgtgtc cgatgtcta 
      gtgctgaaa tcgccgaag gctccgtcg atcccccaa 
      ctaagaagc ggaagcttg actcgtgcc cgatttcaa 
      ccgacatgg cgaacaacg gcgcttccg gatcgtctc 
      agcaggaca tcgacgagg gtctgtact cgcgccagc 
      tctacgtcc tggggcacg acgccatgc ggcgcatgg 
      cctccagcg acgtgctgg tctcaggcc tgggcgggc 
      tcggcgtcg aggtggcca agaacgtca tcctcggcg 
      gggtcaagt ccgtcacgt tgcatgacg aagccgttt 
      gcagcgtcg ccgacctct cgtcgcagt tctacttca 
      ccgaagccg acgtgggca agaacaggg ccgaggtct 
      gctgcaagc agctggcgg agctcaaca cctacgtcc 
      ccacgaagg cctacacgg gccccctga cgccggact 
      tcatccgca agttccgag tcgttgtcc tgaccaata 
      gttgtctcg ctgagcaga tgcaaatca gcgaaatca 
      cacatgcca gtaacattg cgctcattg tggccgata 
      cgagggggc tctttgccc aagtgttct gtgatttcg 
      gggatgctt tcacggtcg ttgacacca acggggagt 
      cgccggtct cggcgatga tcgctgata tttccaccg 
      ataaagaag gcattgtga cctgcattg acgacaccc 
      gccatggca tggaagacg gcgaccacg tcacatttt 
      ccgaagtcc aaggaatga ccgaactga acaattgca 
      agccgatta aaatcaaag tgttaggcc cttacacgt 
      tcagcatcg gcgatacga ccaactttt cgaaatacg 
      agcgtggcg gtattgcca ctcaagtga aaatgccca 
      aaaccttgt cgtttaaat cactcaaag actcgctaa 
      aatcgcccg aatttttga tgaccgatt tcgcgaaat 
      tcgatcatc cacagcagc tccacgtgg cttttcaag 
      cgttgcaca aatacgtcg aaaagcacg gccgtgtac 
      caaaaccgt ggaacaacg aagacgcat ccgaatttc 
      tgtccattg ctaaatctt tggccgtgg acggcggaa 
      acgataccg aagtgaaca ctaacttgt tagaaacgt 
      ttgcgaaag tgtgcgcgg gtgatttga atccgatta 
      atgcgacta tcggtggta tcgtggcgc aggaagtta 
      tgaaagctt gttcgggga aatttcacc cgatttatc 
      agtggttgt atttcgatg cgattgaat gtttgccca 
      cggaagctg gggaaatta ccgaagagt cggcggcac 
      cgaaaggca cacgctatg atggacaaa ttgcggtct 
      tcggaagcg aattccaga agaaattag gcggtttga 
      aatatttcg tagtaggtg ctggagcga tcggttgcg 
      aattactta aaaatttcg ctatgatgg gaattggtg 
      gtgaaggtg gacaaatca ccgtcactg atatggatc 
      ttattgaaa aatctaatc tcaatcgac agtttttgt 
      tcagaccac atgatgttc agcgtccca aatctggaa 
      cggcagcaa aggtgatca agaaaatga acccgagca 
      taaacatag tcgcccatg aaaatcgcg tgggccccg 
      agtctgaaa acatgtacg atgacactt tcttcgagt 
      cgttagacg gcgtagcta acgctttag ataacgttg 
      acgctcgta tctacatgg accgtcgat gtgtctatt 
      accgaaaac ctctactag aatccggaa ccttgggta 
      caaaaggca acacccagg tcgtagtgc ctttcctca 
      ccgaatcct acagttcgt cacaggacc ctcccgaga 
      agagcatcc cgatttgta cccttaaaa atttcccca 
      acgccatcg aacacactc tccaatggg ctcgtgata 
      atttcgaag gccttttca gacagagtg ctgaaaacg 
      catcgcaat atctcaccg atcctgaat ttttggaac 
      gtactatta aattaccag gagttcaac cgattgaag 
      tgctggaat cggtcaagg cagctttag ttgatgata 
      ggccaaaga gttttgaag actgtgtag tgtgggccc 
      gccatcatt gggaggaac aatattcaa atcagatca 
      aacaactgc tttttaatt tcccgccgg atcaaaaaa 
      ccagcactg gacagttgt tctggtcgg gaccgaaga 
      ggtgtcccg aaccactca cattcgatg tgaacaata 
      ccttgcatt tggattatg tttttgcag cggctaatt 
      tgaaagccg aggtttatg gaataccgc agaataggg 
      atcgggctt acatcgccg aagtggtgc aaaaaattc 
      acgtaccag agttcgtcc cgaaatctg gcgtcaaga 
      tcgccgtca ccgattcgc agatggcaa tgacgaacg 
      gttcaagcg tcgacttgg accgcgttg gccaaatca 
      gagaggaat taccttcgg tcgccgaac tgggcaatc 
      tttgcctca ctccacttg atttcgaaa aggacgacg 
      acacaaacc tccacatgg atttcatag tagcggctt 
      cgaatctcc gagccgcca actacaaaa tccctccag 
      ccgatcgtc acaaatcca agctgatcg ccggcaaaa 
      tcatcccag ctatagcga caacaacct cagtcgtcg 
      ccggcctcg tctgtctcg agctttaca aattaacga 
      gaagtttga aatcgttga cgccgttca agaacggtt 
      tcgtcaact tggctctgc cgttcttcg ggttttcgg 
      agcccattg ccgccccca agaacgagt attgcggga 
      aggagtgga cgttgtggg accggtttg aggtggatg 
      gggagatga ctctgagcg agtttttgg agtacttta 
      aagagaagc acggcttgg agatcacga tgttgtcgc 
      agggcgtgt gcatgctgt actcgttct ttatggcca 
      aagcgaagg cgcaggaac gcctggggt tgcccatgt 
      ccgagattg tgaagcgag tgtcgaaga agaagctcg 
      aaccgcacg tcaaggcgc tagtgtttg aactgtgtt 
      gtaacgacg ctgacggaa acgatgtcg aagttccgt 
      atgttaagt ataatctac cttgaatgg ttgcgtgtt 
      ggctgcatt cgtattagg gcatttaat gacagttat 
      taggtgtgc actaaattg aactattct gggtatttg 
      tcaaattgt gatcttgta aattattta tattttttg 
      caagcataa ctagaacac gtgttcggt aggaatttg 
      ttattactt gcccagtga ttctcactt gattttcta 
      ttatttgtg ttatggcga gtgaattca atgagctaa 
      tgcaatata atcgtacgt atgctgata ctggataca 
      ttgtaggtt caggatagt gtttatttt cttaaatat 
      tttgatgtg tgaaattta ataaatatg ttttgaaca 
      t