Status
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Gene information
General information
TC031398
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011395,TC011396
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 829040..833038
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031398
    iBeetle screen iB_01848

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031398 RA
      MMLLKVVLF VSISVCFCD NDNRPLVTT PLGRIRGYH 
      KTSHDGRKF AAFEGIPFA KPPVGQRRF EEPEPVDPW 
      HGTWDARYL TSCAQTSMT QPNVTEGAE DCLHINVYV 
      PREVPTPGE GLDVVVHVH GGAYMYGSG HVYARPDFL 
      MDRDLIFVT FNYRLGVFG FLSTEDEVV PGNMGLKDQ 
      VMALRWVQK NIDSFGGNP NSVTLTGLS AGGSSVHFH 
      YFSPLSEGL FQRGFSQSG AATNCWSLQ EDGLAKAKL 
      LGGSMGCPI GGTTRELVQ CLKERPMEQ ILGKVWLFF 
      KYQFLPFAP FAPVVEEKG PSAFLTDYP FNLLKAGKV 
      LDVPWISSN TQHEGIFPS IFMVNDLEN VNQNWKSLA 
      PAFFDFIYT LPKTQHETA SQQILEYYL GKNQKLTQG 
      NSDQLAKMF TDRIFIIDA ESATKLQAK TAKSPVYYY 
      FFTYFGDFN QVKGVGHGD DAKYFFGNI FEPRPLSEN 
      ELKMKDVML DMLVTYAKK GTPVIKGAD WRPTQGDQL 
      TYLEIGGPE DGQMTLKTS SDLSGAKDF WNSLGLSDT 
      ESFTSMTQQ LRKIMVPHF LILLFISLA QCSRPLVQT 
      PLGAIQGHT KTSHNGRNF SAFEGVPFA KPPVGPKRF 
      EPPEPVDPW HGTWDARHL VTCAQTHMT QPNVTAGSE 
      DCLYVNVYV PGEVPTPGK QLDVVVHIH GGAFMYCSG 
      HYYAKPEFL MDRDLVFVT FNYRLGVFG FLSTEDGVV 
      PGNMGLKDQ VMALRWVQN NIASFGGNP NSVTLTGVS 
      AGGSSVHFH YFSPLSEGL FQRGFSQSG TATNCWSLQ 
      EDGLAKAKL LGGSMGCPI GGTTRELVE CLKERPMEQ 
      ILGKIGLFF KYQFLPFAP FAPVVEEKG PNAFLTDHP 
      YNLLKAGKV LDVPWISSN TQHEGTFPS LSLMNDLDH 
      IYQNWESLA PTFFEFIHT LPPSQHERV SEQILEYYL 
      GKNQKLTQE NSDKFTKMF SDRIFLIDS ESATKLQAK 
      TAKSPVYYY FFTYFGDFN QLKVVGHGD DAKYFFGSV 
      FVPRPLTEN ELKMKDVML DMLVTYAKT GTPVIKGAD 
      WRPTQGDQL TYLDIGGLE DGEIKVKTC GDLSGARDF 
      WNSLGLMDS ENFGSTKEE L
    CDS sequence
    • >TC031398 RA
      atgatgctt ctaaaagta gtactcttt gtctcaatt 
      tccgtgtgt ttttgtgac aatgacaac cgaccattg 
      gtcacaacc ccattgggc cgaatccgc gggtaccac 
      aaaacgtcc catgatggt cgaaagttc gccgctttc 
      gaaggcatc ccatttgcc aaaccacca gtgggacaa 
      agacgcttc gaagaacct gagcctgtg gacccttgg 
      catggtacc tgggacgcc aggtactta accagctgc 
      gcccagacg tccatgacc cagccgaac gtcactgaa 
      ggcgctgag gattgccta cacatcaat gtgtacgta 
      ccaagggaa gtaccaaca cctggggaa gggctagat 
      gtggtggta cacgtccat gggggggct tacatgtat 
      ggtagtggg cacgtgtac gcccgccca gatttccta 
      atggatcgg gacttaatt ttcgtcact tttaactac 
      cgactgggc gtttttggg ttcttgagt accgaggat 
      gaggtggta ccggggaat atgggtttg aaggaccaa 
      gtaatggcc ttgagatgg gtccaaaaa aatattgac 
      agttttggg gggaaccca aacagtgtg accttgacg 
      ggtctatca gctggtggt tccagcgtc catttccat 
      tacttctcc cctttgagt gagggtctc ttccaacga 
      gggttctcc caaagtggt gctgccacc aattgttgg 
      tctttacaa gaggatggg ttggcaaaa gccaaactt 
      ttgggtggt tccatgggt tgtcccatt ggtggtacc 
      actcgtgag ttggttcaa tgtcttaaa gaacgaccc 
      atggagcag attttgggc aaggtttgg ttgttcttc 
      aagtaccag tttttgcca tttgcccct tttgccccc 
      gttgtggag gaaaaaggg ccaagtgcc tttttgacc 
      gactatcct tttaacctc ttaaaggcc gggaaagtg 
      ttagatgtg ccatggatt tcgtcaaac acgcaacac 
      gaagggatt tttccctcg attttcatg gtaaacgat 
      ttagaaaat gttaaccaa aactggaaa tctttagca 
      cccgcattt ttcgatttt atttacact ttgcccaaa 
      actcagcat gagacagct tcccagcaa atcctagag 
      tactatttg ggcaaaaac cagaaactg acccaagga 
      aactccgat cagttggcc aaaatgttc actgaccgt 
      attttcata attgatgcc gaaagtgca actaaactt 
      caggccaaa accgccaaa tcgccagtc tattattac 
      ttttttacc tatttcgga gacttcaac caagtcaaa 
      ggtgtaggt catggtgac gatgccaag tacttcttt 
      ggcaacatt ttcgaaccc agacctttg agtgagaac 
      gaattgaaa atgaaagac gttatgttg gacatgttg 
      gtcacttat gccaaaaaa gggaccccg gtcataaaa 
      ggggccgac tggaggcca actcaagga gaccagtta 
      acgtacctt gaaattggg ggccctgaa gatgggcaa 
      atgacgctc aaaacgagc agtgacctt agcggggcc 
      aaggacttt tggaactct ctaggcttg tcggatact 
      gaaagtttt actagtatg acacaacaa ttacgcaag 
      atcatggta ccacatttc ctaatcctc ttatttatt 
      tcgttggcc cagtgttcg cgcccgttg gtacaaacc 
      ccattgggt gccatccaa ggccacacc aaaacgtcc 
      cacaatggg cggaacttc tccgctttt gaaggagta 
      ccgtttgcc aaaccacca gttggccca aaacgcttc 
      gagccaccg gaacccgtg gacccatgg catggtact 
      tgggacgcc cgtcacctt gtaacatgc gcccaaaca 
      cacatgacc caaccaaac gtgaccgca ggttcggag 
      gattgtctc tacgtcaat gtttacgtc ccgggggaa 
      gtaccaact cctggcaaa caactagat gtcgtggta 
      catatccat ggtggggct tttatgtat tgtagtggg 
      cactattac gccaaacct gagtttctg atggaccgt 
      gacttggtt tttgtcact tttaattac cgactgggc 
      gtttttggg ttcttgagt accgaggat ggggtggta 
      ccggggaat atgggtttg aaggaccaa gttatggcc 
      ttgagatgg gtccaaaac aatattgcc agttttggg 
      gggaaccca aacagtgtg actttgact ggggtatca 
      gctggtggt tccagcgtc cattttcat tatttttcc 
      cctttgagt gagggtctc ttccaacga gggttctcc 
      caaagtggt actgccacc aattgttgg tctttacaa 
      gaggatggg ttggcaaaa gccaaactt ttgggtggt 
      tccatgggt tgtcccatt ggtggtacc actcgtgag 
      ttggttgaa tgtcttaag gaaagaccc atggagcag 
      attttgggc aagattggg ttgttcttc aagtaccag 
      tttttgcca tttgcccct tttgccccc gttgtggag 
      gaaaaaggt ccaaatgcc tttttgacc gaccatcct 
      tacaacctc ctaaaggcc gggaaagtg ttagacgtg 
      ccatggatt tcgtcaaac acgcaacac gaagggact 
      tttccatca ttatctttg atgaacgat ttggaccat 
      atttaccaa aactgggaa agtctagcc cccacattc 
      tttgaattt attcacact ttgccccca agtcaacat 
      gagagagtt tctgagcag attttagag tactatttg 
      ggcaaaaac caaaaactg actcaggaa aattccgat 
      aaatttacc aaaatgttc agcgaccgt attttccta 
      attgattcc gaaagtgca accaaactt caggccaaa 
      accgccaaa tcgccagtc tattattac ttttttacc 
      tattttgga gacttcaac caactcaaa gttgtaggt 
      catggtgac gatgccaag tactttttt ggcagtgtt 
      ttcgtacca agacctttg actgagaac gaattgaaa 
      atgaaggac gttatgttg gacatgttg gtcacttat 
      gccaaaaca gggactccg gtcataaaa ggggccgat 
      tggaggcca actcaagga gaccagttg acatacctt 
      gatattgga ggtcttgaa gatggggaa ataaaggtc 
      aaaacgtgt ggtgacctt agcggggct agggatttt 
      tggaattct ctggggtta atggatagt gaaaatttt 
      ggtagtacc aaggaagaa ttgtaa
    mRNA sequence
    • >TC031398 RA
      agattggag caatttgcg aattaaacg atgagttcc 
      acttatata atgtgacgt aaccttttc ccaccttca 
      ttcctccaa acatgatgc ttctaaaag tagtactct 
      ttgtctcaa tttccgtgt gtttttgtg acaatgaca 
      accgaccat tggtcacaa ccccattgg gccgaatcc 
      gcgggtacc acaaaacgt cccatgatg gtcgaaagt 
      tcgccgctt tcgaaggca tcccatttg ccaaaccac 
      cagtgggac aaagacgct tcgaagaac ctgagcctg 
      tggaccctt ggcatggta cctgggacg ccaggtact 
      taaccagct gcgcccaga cgtccatga cccagccga 
      acgtcactg aaggcgctg aggattgcc tacacatca 
      atgtgtacg taccaaggg aagtaccaa cacctgggg 
      aagggctag atgtggtgg tacacgtcc atggggggg 
      cttacatgt atggtagtg ggcacgtgt acgcccgcc 
      cagatttcc taatggatc gggacttaa ttttcgtca 
      cttttaact accgactgg gcgtttttg ggttcttga 
      gtaccgagg atgaggtgg taccgggga atatgggtt 
      tgaaggacc aagtaatgg ccttgagat gggtccaaa 
      aaaatattg acagttttg gggggaacc caaacagtg 
      tgaccttga cgggtctat cagctggtg gttccagcg 
      tccatttcc attacttct cccctttga gtgagggtc 
      tcttccaac gagggttct cccaaagtg gtgctgcca 
      ccaattgtt ggtctttac aagaggatg ggttggcaa 
      aagccaaac ttttgggtg gttccatgg gttgtccca 
      ttggtggta ccactcgtg agttggttc aatgtctta 
      aagaacgac ccatggagc agattttgg gcaaggttt 
      ggttgttct tcaagtacc agtttttgc catttgccc 
      cttttgccc ccgttgtgg aggaaaaag ggccaagtg 
      cctttttga ccgactatc cttttaacc tcttaaagg 
      ccgggaaag tgttagatg tgccatgga tttcgtcaa 
      acacgcaac acgaaggga tttttccct cgattttca 
      tggtaaacg atttagaaa atgttaacc aaaactgga 
      aatctttag cacccgcat ttttcgatt ttatttaca 
      ctttgccca aaactcagc atgagacag cttcccagc 
      aaatcctag agtactatt tgggcaaaa accagaaac 
      tgacccaag gaaactccg atcagttgg ccaaaatgt 
      tcactgacc gtattttca taattgatg ccgaaagtg 
      caactaaac ttcaggcca aaaccgcca aatcgccag 
      tctattatt actttttta cctatttcg gagacttca 
      accaagtca aaggtgtag gtcatggtg acgatgcca 
      agtacttct ttggcaaca ttttcgaac ccagacctt 
      tgagtgaga acgaattga aaatgaaag acgttatgt 
      tggacatgt tggtcactt atgccaaaa aagggaccc 
      cggtcataa aaggggccg actggaggc caactcaag 
      gagaccagt taacgtacc ttgaaattg ggggccctg 
      aagatgggc aaatgacgc tcaaaacga gcagtgacc 
      ttagcgggg ccaaggact tttggaact ctctaggct 
      tgtcggata ctgaaagtt ttactagta tgacacaac 
      aattacgca agatcatgg taccacatt tcctaatcc 
      tcttattta tttcgttgg cccagtgtt cgcgcccgt 
      tggtacaaa ccccattgg gtgccatcc aaggccaca 
      ccaaaacgt cccacaatg ggcggaact tctccgctt 
      ttgaaggag taccgtttg ccaaaccac cagttggcc 
      caaaacgct tcgagccac cggaacccg tggacccat 
      ggcatggta cttgggacg cccgtcacc ttgtaacat 
      gcgcccaaa cacacatga cccaaccaa acgtgaccg 
      caggttcgg aggattgtc tctacgtca atgtttacg 
      tcccggggg aagtaccaa ctcctggca aacaactag 
      atgtcgtgg tacatatcc atggtgggg cttttatgt 
      attgtagtg ggcactatt acgccaaac ctgagtttc 
      tgatggacc gtgacttgg tttttgtca cttttaatt 
      accgactgg gcgtttttg ggttcttga gtaccgagg 
      atggggtgg taccgggga atatgggtt tgaaggacc 
      aagttatgg ccttgagat gggtccaaa acaatattg 
      ccagttttg gggggaacc caaacagtg tgactttga 
      ctggggtat cagctggtg gttccagcg tccattttc 
      attattttt cccctttga gtgagggtc tcttccaac 
      gagggttct cccaaagtg gtactgcca ccaattgtt 
      ggtctttac aagaggatg ggttggcaa aagccaaac 
      ttttgggtg gttccatgg gttgtccca ttggtggta 
      ccactcgtg agttggttg aatgtctta aggaaagac 
      ccatggagc agattttgg gcaagattg ggttgttct 
      tcaagtacc agtttttgc catttgccc cttttgccc 
      ccgttgtgg aggaaaaag gtccaaatg cctttttga 
      ccgaccatc cttacaacc tcctaaagg ccgggaaag 
      tgttagacg tgccatgga tttcgtcaa acacgcaac 
      acgaaggga cttttccat cattatctt tgatgaacg 
      atttggacc atatttacc aaaactggg aaagtctag 
      cccccacat tctttgaat ttattcaca ctttgcccc 
      caagtcaac atgagagag tttctgagc agattttag 
      agtactatt tgggcaaaa accaaaaac tgactcagg 
      aaaattccg ataaattta ccaaaatgt tcagcgacc 
      gtattttcc taattgatt ccgaaagtg caaccaaac 
      ttcaggcca aaaccgcca aatcgccag tctattatt 
      actttttta cctattttg gagacttca accaactca 
      aagttgtag gtcatggtg acgatgcca agtactttt 
      ttggcagtg ttttcgtac caagacctt tgactgaga 
      acgaattga aaatgaagg acgttatgt tggacatgt 
      tggtcactt atgccaaaa cagggactc cggtcataa 
      aaggggccg attggaggc caactcaag gagaccagt 
      tgacatacc ttgatattg gaggtcttg aagatgggg 
      aaataaagg tcaaaacgt gtggtgacc ttagcgggg 
      ctagggatt tttggaatt ctctggggt taatggata 
      gtgaaaatt ttggtagta ccaaggaag aattgtaat 
      aatttttaa ataaaaaaa atatatatt c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY