Status
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Gene information
General information
TC011390 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 639269..647863
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011390
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01847

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011390 RA
      MEQMTKKET IALRDKHIG KSCQLFFRQ DPLKIMRAK 
      AQYMYDEKG DAYLDCINN VAHVGHCHP AVVEAGCRQ 
      MSILNTNNR FLHDNIVLC AQRIINTMP DKLSVCYFV 
      NSGSEANDL ALRLAQIHT GNRQVITLE HAYHGHLTS 
      LIDISHYKF NLPGGPPQK DYVHVASCP DTYRGKYRD 
      SDYAKEELS KLYSDEILT ICQNVSSEK GEKICAFIA 
      ESLLSCGGQ IIPPDNYMK NVYDHVRQN GGVCIADEV 
      QVGFGRLGK HWWGFQMHD VIPDIVTMG KPMGNGHPI 
      AAVVTTEEI AESFRRTGV EYFNTYGGN PVSCAIANA 
      VFDTIEKEN LREHALVVG EYLFDSCQI LAKKHACIG 
      DVRGVGLFL GIEMVKDRD LRTPDKATA SYVLKRMRE 
      EHILVSADG PECNVIKIK PPMVFTKEN VDEVVSTLD 
      RVLKELRDS QQKSSNVKS TEVPSKEPR TRKSLKEDR 
      IKSI
    CDS sequence
    • >TC011390 RA
      atggaacag atgaccaag aaggagacc attgccctt 
      cgggacaag cacattgga aagtcatgc cagttgttc 
      ttccgtcaa gatccgttg aagataatg agggcgaag 
      gcccagtac atgtatgac gagaaaggc gacgcttat 
      ttggattgt atcaataat gtggcacat gtgggtcat 
      tgccaccca gccgttgtg gaagccggc tgtcgtcaa 
      atgtcaatc ctcaacacc aacaaccgc ttcctgcac 
      gacaacatc gtcctttgc gcccaacgc atcatcaac 
      acaatgcct gacaaactc tcagtttgc tattttgtc 
      aattctggc tcagaagcc aacgatttg gctctgcgt 
      cttgcgcaa attcacacc ggaaatcgt caagtcatc 
      actttggag catgcgtac catggccat ttgacttcg 
      ttgattgac atttcccac tataagttt aatcttcct 
      ggtgggcca ccacagaaa gactatgtc catgtggct 
      tcctgtccc gatacttat cgaggaaag taccgcgat 
      tccgactat gccaaagaa gaactgtca aaactatac 
      tctgacgaa attttgaca atctgccaa aacgtttcg 
      tccgaaaaa ggtgaaaaa atctgcgcc tttattgct 
      gaaagtctg ctaagttgt ggcggtcaa attatcccg 
      cctgataat tacatgaaa aatgtctac gatcacgtc 
      cgacaaaat ggcggcgtt tgcatcgcc gacgaggtc 
      caagtcggc tttggacgt ttgggtaaa cactggtgg 
      ggttttcaa atgcatgac gtcatccca gatattgta 
      acaatgggt aaacccatg ggtaatggg catcccatt 
      gctgcagtg gtgacaact gaagaaatt gccgaaagt 
      tttcggcga actggagtc gagtacttt aatacgtat 
      ggtggtaat cctgtttcg tgcgccata gccaacgct 
      gtttttgac acaatcgag aaggagaat ttacgagag 
      catgcactc gtcgttgga gaatattta tttgacagc 
      tgtcaaatt ctcgccaag aaacacgcc tgtattggc 
      gatgtgcgt ggtgttggt ttgtttctg gggattgaa 
      atggttaag gaccgtgat ttgcgcacg cctgataag 
      gccacagcc agctatgtg ctaaaaagg atgagggag 
      gagcatatt ttggtcagt gctgacggt ccggaatgc 
      aatgttata aaaataaaa cccccaatg gtttttacg 
      aaggaaaat gtggatgag gttgtttcc actcttgat 
      cgtgttttg aaggagttg agggatagt cagcagaaa 
      tcgtcgaat gtgaaaagc actgaagtt ccgagtaaa 
      gagcctcgg acgcggaag tcgctcaag gaggatagg 
      attaagtcg atttaa
    mRNA sequence
    • >TC011390 RA
      aatatttta atcactcga taacacaat ttgttttgc 
      gataaaacc atccaaatt taccctcga accgatcaa 
      tattgacca aataaccac cccaaagtc tcttgtttt 
      tgtctcgaa aaaaatgtg aaaattcca cctggtggt 
      atcacctct gttatcaca aaggttacg tgctatcac 
      accgtcgtt tcgtaacag caatgcgtg cgcacaaat 
      tccataata aatagcgca acgcaacgc aacacccag 
      caaagcgct ccagacacg cttccaggc ccacatcca 
      gcacccatc atggaacag atgaccaag aaggagacc 
      attgccctt cgggacaag cacattgga aagtcatgc 
      cagttgttc ttccgtcaa gatccgttg aagataatg 
      agggcgaag gcccagtac atgtatgac gagaaaggc 
      gacgcttat ttggattgt atcaataat gtggcacat 
      gtgggtcat tgccaccca gccgttgtg gaagccggc 
      tgtcgtcaa atgtcaatc ctcaacacc aacaaccgc 
      ttcctgcac gacaacatc gtcctttgc gcccaacgc 
      atcatcaac acaatgcct gacaaactc tcagtttgc 
      tattttgtc aattctggc tcagaagcc aacgatttg 
      gctctgcgt cttgcgcaa attcacacc ggaaatcgt 
      caagtcatc actttggag catgcgtac catggccat 
      ttgacttcg ttgattgac atttcccac tataagttt 
      aatcttcct ggtgggcca ccacagaaa gactatgtc 
      catgtggct tcctgtccc gatacttat cgaggaaag 
      taccgcgat tccgactat gccaaagaa gaactgtca 
      aaactatac tctgacgaa attttgaca atctgccaa 
      aacgtttcg tccgaaaaa ggtgaaaaa atctgcgcc 
      tttattgct gaaagtctg ctaagttgt ggcggtcaa 
      attatcccg cctgataat tacatgaaa aatgtctac 
      gatcacgtc cgacaaaat ggcggcgtt tgcatcgcc 
      gacgaggtc caagtcggc tttggacgt ttgggtaaa 
      cactggtgg ggttttcaa atgcatgac gtcatccca 
      gatattgta acaatgggt aaacccatg ggtaatggg 
      catcccatt gctgcagtg gtgacaact gaagaaatt 
      gccgaaagt tttcggcga actggagtc gagtacttt 
      aatacgtat ggtggtaat cctgtttcg tgcgccata 
      gccaacgct gtttttgac acaatcgag aaggagaat 
      ttacgagag catgcactc gtcgttgga gaatattta 
      tttgacagc tgtcaaatt ctcgccaag aaacacgcc 
      tgtattggc gatgtgcgt ggtgttggt ttgtttctg 
      gggattgaa atggttaag gaccgtgat ttgcgcacg 
      cctgataag gccacagcc agctatgtg ctaaaaagg 
      atgagggag gagcatatt ttggtcagt gctgacggt 
      ccggaatgc aatgttata aaaataaaa cccccaatg 
      gtttttacg aaggaaaat gtggatgag gttgtttcc 
      actcttgat cgtgttttg aaggagttg agggatagt 
      cagcagaaa tcgtcgaat gtgaaaagc actgaagtt 
      ccgagtaaa gagcctcgg acgcggaag tcgctcaag 
      gaggatagg attaagtcg atttaattt attgttcag 
      tattttgta tgttttctg tgaaaataa aactattgt 
      gaagct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY