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Gene information
General information
TC011388 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 613849..615959
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011388
    iBeetle screen iB_01846

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg posterior protruding - Number of animals showing the phenotype: 4
    mature egg posterior colour darker - Number of animals showing the phenotype: 4
    mature egg number increased - Number of animals showing the phenotype: 4

    egg chamber number decreased - Number of animals showing the phenotype: 4

    mature egg deposition irregular - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011388 RA
      MSYIVSPDV VKYACTCGL LKPISCLYF CRYCCELRC 
      GFCVCHEVD SHFCGKCCE NLPSSEARL KKNRCGNCY 
      ICPCCQQEL STRIGSKGP SNPEDPKST PKKMYYLSC 
      LYCRWSSRD VGIPDQGAA TGGWPEREN VHANRLQEV 
      IEGYKAFIT AQRQQKERE KKKQRGKFI SYTDRTGVT 
      AAALRKRIG LPAEVPNTY YRNRPKPPE VAIAREEVE 
      DFPEDLLTK ALNLMEITT IDQRLLQPD LQPTTVDKL 
      FPIHKQLSI KRSLRCRSC EHNVSKPEY NPNSVKFKI 
      QLFAYYHIP EVRIVTVEP LRAGKTAEL ILKFTNPTQ 
      HQTVITFLP LDLTDKGGD GDREVVGET QSLNLPEKQ 
      PPSLGGQPS SLLSLSSRQ PSITIKPRP IRQATNCSI 
      AIPGSKVIL PPRDDAAEY DDSGETHNI QDDTKLVVW 
      RKSNKAFVK LAVTPDDGL ELNTEIVAG FTMQHIYTN 
      TIATTVENK EPDRHDHNV RVFLSLGNL VGSE
    CDS sequence
    • >TC011388 RA
      atgtcgtac atcgtaagc cctgatgta gtgaaatat 
      gcgtgcact tgtggcctc ctcaagccc atttcgtgc 
      ctttacttc tgccggtat tgttgcgag cttcggtgc 
      ggcttttgc gtgtgccac gaggtcgat tcccacttt 
      tgtggaaaa tgctgcgag aatctgccc tcgtccgaa 
      gcccggctc aagaagaac aggtgtggc aactgctac 
      atctgcccc tgttgccag caggagctc tccactagg 
      attggctcg aaggggccc tccaatccg gaggacccc 
      aagtccact cccaagaaa atgtactac ttgtcctgt 
      ctgtactgt cgctggagc tcccgtgat gtgggcatt 
      ccggaccag ggggctgcc accggcggc tggcccgag 
      cgcgagaat gtgcacgcg aatcgcctc caggaggtg 
      attgagggc tataaggcc ttcattacg gcacagagg 
      cagcaaaag gaacgggag aagaagaag caaaggggg 
      aagtttatt agctacacg gaccgtact ggggtgacg 
      gccgccgcc ctgcgcaag cgcatcggg ctgccggcc 
      gaagttcca aacacgtac taccggaac cggccgaag 
      ccgcccgaa gtggccatc gcgcgtgag gaagttgag 
      gacttcccc gaagatctc ctcaccaaa gccttaaac 
      ttgatggaa atcacaaca atcgatcaa cgcctactc 
      caacccgac ttgcagcca acgaccgtc gataaatta 
      ttcccaatt cataagcag ctgtcgatc aagcgatcg 
      ctgaggtgt cgctcgtgc gaacacaac gtcagcaaa 
      cccgaatat aaccccaac agtgtcaag tttaaaatc 
      cagttattt gcatattat cacattccg gaggtgcga 
      attgtcaca gttgaacct ctacgtgcc ggaaaaacg 
      gccgaattg attttgaaa tttacaaat ccgacgcaa 
      caccaaact gtgattacg ttcctccca ttggatctg 
      acagataag ggaggggat ggcgatcgg gaagtggtg 
      ggggaaacg caatctttg aacctacca gagaaacag 
      cccccttca ttggggggc caaccttcc agtctttta 
      agtttatct tcccgtcag ccgtcaatc acgatcaag 
      ccacgcccc attcgccaa gcgaccaat tgcagcatc 
      gctataccg ggcagtaag gttatactg ccgccgcga 
      gatgacgct gctgagtat gacgattcc ggggaaaca 
      cataatatt caagatgat accaagctt gtagtttgg 
      agaaaatcg aacaaggcc ttcgtcaag ttggctgtg 
      actcctgat gatggtctt gaattgaat accgaaatc 
      gtggccggt tttacaatg caacatatt tacacaaat 
      acgattgcg acaactgtg gagaacaag gaacccgat 
      agacacgac cacaatgtt agggttttt ctgagtttg 
      gggaatttg gtcgggtct gaatga
    mRNA sequence
    • >TC011388 RA
      cctctagtg tcaagtaag ggggctcca agatgtcgt 
      acatcgtaa gccctgatg tagtgaaat atgcgtgca 
      cttgtggcc tcctcaagc ccatttcgt gcctttact 
      tctgccggt attgttgcg agcttcggt gcggctttt 
      gcgtgtgcc acgaggtcg attcccact tttgtggaa 
      aatgctgcg agaatctgc cctcgtccg aagcccggc 
      tcaagaaga acaggtgtg gcaactgct acatctgcc 
      cctgttgcc agcaggagc tctccacta ggattggct 
      cgaaggggc cctccaatc cggaggacc ccaagtcca 
      ctcccaaga aaatgtact acttgtcct gtctgtact 
      gtcgctgga gctcccgtg atgtgggca ttccggacc 
      agggggctg ccaccggcg gctggcccg agcgcgaga 
      atgtgcacg cgaatcgcc tccaggagg tgattgagg 
      gctataagg ccttcatta cggcacaga ggcagcaaa 
      aggaacggg agaagaaga agcaaaggg ggaagttta 
      ttagctaca cggaccgta ctggggtga cggccgccg 
      ccctgcgca agcgcatcg ggctgccgg ccgaagttc 
      caaacacgt actaccgga accggccga agccgcccg 
      aagtggcca tcgcgcgtg aggaagttg aggacttcc 
      ccgaagatc tcctcacca aagccttaa acttgatgg 
      aaatcacaa caatcgatc aacgcctac tccaacccg 
      acttgcagc caacgaccg tcgataaat tattcccaa 
      ttcataagc agctgtcga tcaagcgat cgctgaggt 
      gtcgctcgt gcgaacaca acgtcagca aacccgaat 
      ataacccca acagtgtca agtttaaaa tccagttat 
      ttgcatatt atcacattc cggaggtgc gaattgtca 
      cagttgaac ctctacgtg ccggaaaaa cggccgaat 
      tgattttga aatttacaa atccgacgc aacaccaaa 
      ctgtgatta cgttcctcc cattggatc tgacagata 
      agggagggg atggcgatc gggaagtgg tgggggaaa 
      cgcaatctt tgaacctac cagagaaac agccccctt 
      cattggggg gccaacctt ccagtcttt taagtttat 
      cttcccgtc agccgtcaa tcacgatca agccacgcc 
      ccattcgcc aagcgacca attgcagca tcgctatac 
      cgggcagta aggttatac tgccgccgc gagatgacg 
      ctgctgagt atgacgatt ccggggaaa cacataata 
      ttcaagatg ataccaagc ttgtagttt ggagaaaat 
      cgaacaagg ccttcgtca agttggctg tgactcctg 
      atgatggtc ttgaattga ataccgaaa tcgtggccg 
      gttttacaa tgcaacata tttacacaa atacgattg 
      cgacaactg tggagaaca aggaacccg atagacacg 
      accacaatg ttagggttt ttctgagtt tggggaatt 
      tggtcgggt ctgaatgaa tcttgtact ggtcccact 
      attataagt tataaaata atgttttgt attattcac 
      tcaattaat gaattgtat tattgcttc agtaacaaa 
      caataaagt tatgctttt tca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY