General information | |||
TC011388 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015450233.1: 613849..615959 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC011388 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01846 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)mature egg posterior colour darker - Number of animals showing the phenotype: 4
mature egg number increased - Number of animals showing the phenotype: 4
egg chamber number decreased - Number of animals showing the phenotype: 4
mature egg deposition irregular - Number of animals showing the phenotype: 4
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 4 (11 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 8 (16 dpi)
- >TC011388 RA
MSYIVSPDV VKYACTCGL LKPISCLYF CRYCCELRC GFCVCHEVD SHFCGKCCE NLPSSEARL KKNRCGNCY ICPCCQQEL STRIGSKGP SNPEDPKST PKKMYYLSC LYCRWSSRD VGIPDQGAA TGGWPEREN VHANRLQEV IEGYKAFIT AQRQQKERE KKKQRGKFI SYTDRTGVT AAALRKRIG LPAEVPNTY YRNRPKPPE VAIAREEVE DFPEDLLTK ALNLMEITT IDQRLLQPD LQPTTVDKL FPIHKQLSI KRSLRCRSC EHNVSKPEY NPNSVKFKI QLFAYYHIP EVRIVTVEP LRAGKTAEL ILKFTNPTQ HQTVITFLP LDLTDKGGD GDREVVGET QSLNLPEKQ PPSLGGQPS SLLSLSSRQ PSITIKPRP IRQATNCSI AIPGSKVIL PPRDDAAEY DDSGETHNI QDDTKLVVW RKSNKAFVK LAVTPDDGL ELNTEIVAG FTMQHIYTN TIATTVENK EPDRHDHNV RVFLSLGNL VGSE
- >TC011388 RA
atgtcgtac atcgtaagc cctgatgta gtgaaatat gcgtgcact tgtggcctc ctcaagccc atttcgtgc ctttacttc tgccggtat tgttgcgag cttcggtgc ggcttttgc gtgtgccac gaggtcgat tcccacttt tgtggaaaa tgctgcgag aatctgccc tcgtccgaa gcccggctc aagaagaac aggtgtggc aactgctac atctgcccc tgttgccag caggagctc tccactagg attggctcg aaggggccc tccaatccg gaggacccc aagtccact cccaagaaa atgtactac ttgtcctgt ctgtactgt cgctggagc tcccgtgat gtgggcatt ccggaccag ggggctgcc accggcggc tggcccgag cgcgagaat gtgcacgcg aatcgcctc caggaggtg attgagggc tataaggcc ttcattacg gcacagagg cagcaaaag gaacgggag aagaagaag caaaggggg aagtttatt agctacacg gaccgtact ggggtgacg gccgccgcc ctgcgcaag cgcatcggg ctgccggcc gaagttcca aacacgtac taccggaac cggccgaag ccgcccgaa gtggccatc gcgcgtgag gaagttgag gacttcccc gaagatctc ctcaccaaa gccttaaac ttgatggaa atcacaaca atcgatcaa cgcctactc caacccgac ttgcagcca acgaccgtc gataaatta ttcccaatt cataagcag ctgtcgatc aagcgatcg ctgaggtgt cgctcgtgc gaacacaac gtcagcaaa cccgaatat aaccccaac agtgtcaag tttaaaatc cagttattt gcatattat cacattccg gaggtgcga attgtcaca gttgaacct ctacgtgcc ggaaaaacg gccgaattg attttgaaa tttacaaat ccgacgcaa caccaaact gtgattacg ttcctccca ttggatctg acagataag ggaggggat ggcgatcgg gaagtggtg ggggaaacg caatctttg aacctacca gagaaacag cccccttca ttggggggc caaccttcc agtctttta agtttatct tcccgtcag ccgtcaatc acgatcaag ccacgcccc attcgccaa gcgaccaat tgcagcatc gctataccg ggcagtaag gttatactg ccgccgcga gatgacgct gctgagtat gacgattcc ggggaaaca cataatatt caagatgat accaagctt gtagtttgg agaaaatcg aacaaggcc ttcgtcaag ttggctgtg actcctgat gatggtctt gaattgaat accgaaatc gtggccggt tttacaatg caacatatt tacacaaat acgattgcg acaactgtg gagaacaag gaacccgat agacacgac cacaatgtt agggttttt ctgagtttg gggaatttg gtcgggtct gaatga
- >TC011388 RA
cctctagtg tcaagtaag ggggctcca agatgtcgt acatcgtaa gccctgatg tagtgaaat atgcgtgca cttgtggcc tcctcaagc ccatttcgt gcctttact tctgccggt attgttgcg agcttcggt gcggctttt gcgtgtgcc acgaggtcg attcccact tttgtggaa aatgctgcg agaatctgc cctcgtccg aagcccggc tcaagaaga acaggtgtg gcaactgct acatctgcc cctgttgcc agcaggagc tctccacta ggattggct cgaaggggc cctccaatc cggaggacc ccaagtcca ctcccaaga aaatgtact acttgtcct gtctgtact gtcgctgga gctcccgtg atgtgggca ttccggacc agggggctg ccaccggcg gctggcccg agcgcgaga atgtgcacg cgaatcgcc tccaggagg tgattgagg gctataagg ccttcatta cggcacaga ggcagcaaa aggaacggg agaagaaga agcaaaggg ggaagttta ttagctaca cggaccgta ctggggtga cggccgccg ccctgcgca agcgcatcg ggctgccgg ccgaagttc caaacacgt actaccgga accggccga agccgcccg aagtggcca tcgcgcgtg aggaagttg aggacttcc ccgaagatc tcctcacca aagccttaa acttgatgg aaatcacaa caatcgatc aacgcctac tccaacccg acttgcagc caacgaccg tcgataaat tattcccaa ttcataagc agctgtcga tcaagcgat cgctgaggt gtcgctcgt gcgaacaca acgtcagca aacccgaat ataacccca acagtgtca agtttaaaa tccagttat ttgcatatt atcacattc cggaggtgc gaattgtca cagttgaac ctctacgtg ccggaaaaa cggccgaat tgattttga aatttacaa atccgacgc aacaccaaa ctgtgatta cgttcctcc cattggatc tgacagata agggagggg atggcgatc gggaagtgg tgggggaaa cgcaatctt tgaacctac cagagaaac agccccctt cattggggg gccaacctt ccagtcttt taagtttat cttcccgtc agccgtcaa tcacgatca agccacgcc ccattcgcc aagcgacca attgcagca tcgctatac cgggcagta aggttatac tgccgccgc gagatgacg ctgctgagt atgacgatt ccggggaaa cacataata ttcaagatg ataccaagc ttgtagttt ggagaaaat cgaacaagg ccttcgtca agttggctg tgactcctg atgatggtc ttgaattga ataccgaaa tcgtggccg gttttacaa tgcaacata tttacacaa atacgattg cgacaactg tggagaaca aggaacccg atagacacg accacaatg ttagggttt ttctgagtt tggggaatt tggtcgggt ctgaatgaa tcttgtact ggtcccact attataagt tataaaata atgttttgt attattcac tcaattaat gaattgtat tattgcttc agtaacaaa caataaagt tatgctttt tca
