Status
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Gene information
General information
TC011384 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 540342..541662
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011384
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01845

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011384 RA
      MLISAGLAL KIHPFGPQG VDATARPQL SAASKIIQV 
      STCNDNTYL LILRENEKP AIFSAKDKS NIRLIHTIN 
      VSNVTTLTF KHTTKKAIA LGTKTGDVL IYDTKNRTV 
      ANTYPKISD EIKFLDFTA NDKHLCGLD ASTLLVITD 
      LGANPSVKK YKQAVECVL MRCHPFVAN RVAVGLSTH 
      TVVLWDVQT GSELVSLGL GESPVSGIA MSSCGNYLV 
      MCGDCIKIY GIDFVSGNH TFQFHVDMD SRVTCMDLS 
      PDDRYVAIG LSNGSVRLY DVKQNMLMV SEARVHNEA 
      ISTLVFEHE TFESNSHFS SMVKLSELD VKNRSGEEK 
      SVKVAEGDD LEKFKNRVL KLVKCEMDR LESQLDEHC 
      DKFQKFLDN EFEAIDSII SKKWNIFTD GDMNQIMKA 
      ICPEEARSS VD
    CDS sequence
    • >TC011384 RA
      atgttaatc tccgcgggc ctcgccctc aaaatccac 
      cccttcggc ccccagggg gtggacgcc accgcccgc 
      ccccagctc tccgccgcc tccaaaatc atccaagtg 
      tccacgtgt aacgacaac acctatcta ctcattttg 
      cgagaaaac gaaaaaccg gcgattttc tccgccaaa 
      gacaagtcc aacatccgt ctgattcac accatcaac 
      gtttcgaac gtcacgacg ctcactttc aagcacacg 
      accaagaaa gccatagct ttgggcaca aaaaccggc 
      gatgtgttg atttatgac acgaaaaat cgcacggtt 
      gcaaacaca tacccgaaa atcagtgac gaaatcaag 
      tttttggac tttacggcc aatgacaag catttgtgc 
      ggtttggac gcgagtact ttactcgta attacagat 
      ttgggggcg aatccatcg gttaaaaag tacaaacaa 
      gcagtggag tgtgtttta atgcgttgc catcctttt 
      gtggcgaat cgagtggcc gtgggttta agtactcac 
      acggtggtg ctgtgggac gtccaaacc gggagtgaa 
      ctcgtgagt ttaggactt ggggagtcc ccagtgagt 
      gggattgca atgtcgtca tgtggcaac tacttggtc 
      atgtgtggc gattgtata aaaatctac gggattgat 
      tttgtttcg gggaatcac acgttccag ttccacgtg 
      gatatggac tcgcgggtc acgtgtatg gacttaagt 
      ccggatgat cggtacgtt gccattgga ctgagtaat 
      ggcagtgtg cggttgtat gacgtgaaa cagaacatg 
      ttgatggtt tccgaggcc agagtacat aacgaggcc 
      ataagtact ctagtgttt gaacacgaa acgttcgag 
      tccaattcg catttctcc tcgatggtg aagttgtcc 
      gaactggat gtgaagaat aggagtggg gaggagaaa 
      agtgtgaag gtggcagaa ggagatgat ttggagaag 
      ttcaaaaat cgagtcctt aaacttgtc aagtgtgag 
      atggatcgc ttggagagc caacttgat gagcattgt 
      gacaagttt cagaagttt ctggataat gagtttgaa 
      gccattgat agcattatt agcaagaag tggaatatt 
      tttacggat ggggatatg aaccagatc atgaaggcc 
      atttgtcca gaggaggcg aggagttcg gtcgactag
    mRNA sequence
    • >TC011384 RA
      aaattcccc caaaattga aatgttaat ctccgcggg 
      cctcgccct caaaatcca ccccttcgg cccccaggg 
      ggtggacgc caccgcccg cccccagct ctccgccgc 
      ctccaaaat catccaagt gtccacgtg taacgacaa 
      cacctatct actcatttt gcgagaaaa cgaaaaacc 
      ggcgatttt ctccgccaa agacaagtc caacatccg 
      tctgattca caccatcaa cgtttcgaa cgtcacgac 
      gctcacttt caagcacac gaccaagaa agccatagc 
      tttgggcac aaaaaccgg cgatgtgtt gatttatga 
      cacgaaaaa tcgcacggt tgcaaacac atacccgaa 
      aatcagtga cgaaatcaa gtttttgga ctttacggc 
      caatgacaa gcatttgtg cggtttgga cgcgagtac 
      tttactcgt aattacaga tttgggggc gaatccatc 
      ggttaaaaa gtacaaaca agcagtgga gtgtgtttt 
      aatgcgttg ccatccttt tgtggcgaa tcgagtggc 
      cgtgggttt aagtactca cacggtggt gctgtggga 
      cgtccaaac cgggagtga actcgtgag tttaggact 
      tggggagtc cccagtgag tgggattgc aatgtcgtc 
      atgtggcaa ctacttggt catgtgtgg cgattgtat 
      aaaaatcta cgggattga ttttgtttc ggggaatca 
      cacgttcca gttccacgt ggatatgga ctcgcgggt 
      cacgtgtat ggacttaag tccggatga tcggtacgt 
      tgccattgg actgagtaa tggcagtgt gcggttgta 
      tgacgtgaa acagaacat gttgatggt ttccgaggc 
      cagagtaca taacgaggc cataagtac tctagtgtt 
      tgaacacga aacgttcga gtccaattc gcatttctc 
      ctcgatggt gaagttgtc cgaactgga tgtgaagaa 
      taggagtgg ggaggagaa aagtgtgaa ggtggcaga 
      aggagatga tttggagaa gttcaaaaa tcgagtcct 
      taaacttgt caagtgtga gatggatcg cttggagag 
      ccaacttga tgagcattg tgacaagtt tcagaagtt 
      tctggataa tgagtttga agccattga tagcattat 
      tagcaagaa gtggaatat ttttacgga tggggatat 
      gaaccagat catgaaggc catttgtcc agaggaggc 
      gaggagttc ggtcgacta gtgattggg ataaaaaaa 
      aaatttgtt ttttttgtc aaaatattt ttttgaaaa 
      ttgttaata aagaaatac ttatatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY