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Gene information
General information
TC011344 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3994275..3998674
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011344
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01843

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    remarks: later than schedule,
    Pupal morphology
    remarks: later than schedule,
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larva appendages shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larva dorsal bent - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva bristle mostlynumber decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head capsule shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs segment mostly fused - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: seems that trochanter & coxa are missing
    abdomen ectopically segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: whole abdomen appears crumpled-up -> no segments identifyable
    abdomen dorsalnot fused - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011344 RA
      MEGNQMCIL AAQLRGSAN NKSNILTLS TRTLDTILS 
      SFELLEEQS TSFHVKQPH NFKAELLRD LQYLSNYIQ 
      ETKTLKLYI DSYDDTPEI DITKFRKLI SLELSKVDV 
      NTVKGLQLL RSQLQKLSC HKTQNNLSD ILINCGGDK 
      SRALCWNEL KEAACTGNN IVELDLSLE CAPWLHTLD 
      LSHNEITNF EALSCLVNL KYLNLSYNR LEGVSVLRG 
      QVCNRLQNL ILKNNFIED IAGLRALTN LWVLDLSNN 
      CLVDHKSLI ALSHLAALQ WLNLQSNPL SFHPNHRNR 
      TASYLHVNT ATTHFVLNS AVLSKNEKK LVGSYYPLQ 
      MRTNSFSTD SLSLTTEKA RRVRHVVIE EENTVRESS 
      IPSLPPTPK QYLETKKQI EDLHEKFGE SWLNSQSGL 
      LDVLGLESF SLPLSTSPY EADFTIYSG DFTQEQEIA 
      KGTVVEPPD QINEEYEKK IEAIPDEPD IEGEESIYL 
      ATVRTETEP IFIVIGQTH LSERECSTG NEREKWHLD 
      SITNCEILD EGLTTVQID FETMRKSRK QRIYVFEDE 
      IEAFVACIK NKINTRQPK KEDDVKYQC MKCSTVFGR 
      VKNESILGE AAVKCPSCD STLVVEEV
    CDS sequence
    • >TC011344 RA
      atggagggc aaccagatg tgtattctt gcagcacaa 
      cttcgtggc tcagccaat aacaagtcc aacatccta 
      acattgtcc acacgaaca ctagatacg attttgtcg 
      tcatttgag ctccttgaa gaacagtca acttcgttc 
      catgtcaag cagccccac aacttcaag gctgaactc 
      ctccgagac ttgcaatac ctctccaac tacatacaa 
      gaaaccaaa accttgaaa ctatacatc gacagctat 
      gatgacacc cccgaaatc gacatcaca aaattccgg 
      aaactcatc tctttagaa ttgtccaaa gtggacgtt 
      aacactgtt aaaggcctc cagttgtta cgctcgcaa 
      ttacaaaaa ttaagttgc cacaagact caaaataat 
      ttaagcgat attttgata aattgtggg ggcgataag 
      agccgggct ttgtgctgg aacgaactg aaggaagcc 
      gcctgcact ggtaataac atagtggag ctagactta 
      agtcttgag tgtgccccc tggctccac acgctcgat 
      ttgagccac aacgagatc acgaatttt gaagctttg 
      agctgtctt gtcaattta aagtacctg aatttgtct 
      tacaacagg ctggagggc gtctcggtg ctcagagga 
      caggtttgc aaccgctta cagaatttg atcctaaaa 
      aacaacttt atcgaagac atcgccggt ttgcgcgcc 
      ctaacaaac ctctgggtg ctcgacttg agcaacaat 
      tgcctagtg gaccacaaa tcgctcatc gccctatct 
      cacttggcc gccctccag tggctcaac ttgcaaagc 
      aaccccttg agcttccac cccaatcac cgcaaccgc 
      actgccagc tacctccac gtcaacact gcaaccacc 
      cactttgtg ctaaacagc gccgttttg agcaaaaac 
      gagaagaaa ttggtcggc tcctactac cctctccag 
      atgagaact aacagtttt tccaccgat tctctgagt 
      cttacgacg gagaaagcc aggagggtg cggcacgtg 
      gtcattgag gaggagaac acagtgagg gagagcagc 
      atcccctca ctgccgccc accccaaag caatatttg 
      gaaacaaag aaacaaatc gaagatttg cacgaaaaa 
      ttcggcgag tcgtggctg aactcacag tcggggctg 
      ttggacgtg ctggggctg gagagtttc tcgctcccg 
      ttgagcaca agtccgtac gaggccgac tttacgatc 
      tattccggc gattttacc caagaacaa gaaatagca 
      aaaggcact gttgtggag ccccctgac cagattaac 
      gaagaatac gagaagaaa atcgaagca atcccggac 
      gagcccgac attgaaggg gaagaaagc atttacttg 
      gccactgtt cgaacagaa actgagccc attttcata 
      gtaatcggg caaacgcac ctaagtgag agggagtgc 
      tccactggg aacgaacgc gaaaagtgg cacctggac 
      agcatcacc aactgcgag atcctggac gaggggctc 
      acaacagtt caaatcgac ttcgaaacg atgaggaaa 
      agccgcaag caacgaatc tacgtcttc gaggacgaa 
      atcgaagcc tttgtcgcg tgcatcaaa aacaaaatc 
      aacacgcga cagccgaag aaagaggac gatgtcaag 
      tatcagtgc atgaagtgt tcgactgtg ttcgggaga 
      gtgaaaaac gagtcgatt ttgggggaa gccgcggtc 
      aagtgccca agctgtgat agtactttg gtggtggag 
      gaggtctaa
    mRNA sequence
    • >TC011344 RA
      agaaaacga aaaattata gtatagtgc acccattaa 
      ttaatttat ggccaattt aaataatta acaattagg 
      caattggcg cataaatta aaaaaatgt ttatgataa 
      ggcattttg acaggtgac aggtgccaa gtgtcacaa 
      caaacaaaa aggttataa tttgataag aaatgcccc 
      cgaggttgg gagatcccc acatttcgg tgataaaaa 
      gcgcacttg caaattttc gtgtcgttt gggattcgt 
      ttctcatct ggtcataaa aatgcgata tggagggca 
      accagatgt gtattcttg cagcacaac ttcgtggct 
      cagccaata acaagtcca acatcctaa cattgtcca 
      cacgaacac tagatacga ttttgtcgt catttgagc 
      tccttgaag aacagtcaa cttcgttcc atgtcaagc 
      agccccaca acttcaagg ctgaactcc tccgagact 
      tgcaatacc tctccaact acatacaag aaaccaaaa 
      ccttgaaac tatacatcg acagctatg atgacaccc 
      ccgaaatcg acatcacaa aattccgga aactcatct 
      ctttagaat tgtccaaag tggacgtta acactgtta 
      aaggcctcc agttgttac gctcgcaat tacaaaaat 
      taagttgcc acaagactc aaaataatt taagcgata 
      ttttgataa attgtgggg gcgataaga gccgggctt 
      tgtgctgga acgaactga aggaagccg cctgcactg 
      gtaataaca tagtggagc tagacttaa gtcttgagt 
      gtgccccct ggctccaca cgctcgatt tgagccaca 
      acgagatca cgaattttg aagctttga gctgtcttg 
      tcaatttaa agtacctga atttgtctt acaacaggc 
      tggagggcg tctcggtgc tcagaggac aggtttgca 
      accgcttac agaatttga tcctaaaaa acaacttta 
      tcgaagaca tcgccggtt tgcgcgccc taacaaacc 
      tctgggtgc tcgacttga gcaacaatt gcctagtgg 
      accacaaat cgctcatcg ccctatctc acttggccg 
      ccctccagt ggctcaact tgcaaagca accccttga 
      gcttccacc ccaatcacc gcaaccgca ctgccagct 
      acctccacg tcaacactg caaccaccc actttgtgc 
      taaacagcg ccgttttga gcaaaaacg agaagaaat 
      tggtcggct cctactacc ctctccaga tgagaacta 
      acagttttt ccaccgatt ctctgagtc ttacgacgg 
      agaaagcca ggagggtgc ggcacgtgg tcattgagg 
      aggagaaca cagtgaggg agagcagca tcccctcac 
      tgccgccca ccccaaagc aatatttgg aaacaaaga 
      aacaaatcg aagatttgc acgaaaaat tcggcgagt 
      cgtggctga actcacagt cggggctgt tggacgtgc 
      tggggctgg agagtttct cgctcccgt tgagcacaa 
      gtccgtacg aggccgact ttacgatct attccggcg 
      attttaccc aagaacaag aaatagcaa aaggcactg 
      ttgtggagc cccctgacc agattaacg aagaatacg 
      agaagaaaa tcgaagcaa tcccggacg agcccgaca 
      ttgaagggg aagaaagca tttacttgg ccactgttc 
      gaacagaaa ctgagccca ttttcatag taatcgggc 
      aaacgcacc taagtgaga gggagtgct ccactggga 
      acgaacgcg aaaagtggc acctggaca gcatcacca 
      actgcgaga tcctggacg aggggctca caacagttc 
      aaatcgact tcgaaacga tgaggaaaa gccgcaagc 
      aacgaatct acgtcttcg aggacgaaa tcgaagcct 
      ttgtcgcgt gcatcaaaa acaaaatca acacgcgac 
      agccgaaga aagaggacg atgtcaagt atcagtgca 
      tgaagtgtt cgactgtgt tcgggagag tgaaaaacg 
      agtcgattt tgggggaag ccgcggtca agtgcccaa 
      gctgtgata gtactttgg tggtggagg aggtctaat 
      tgactgatt caacaattt atccttcag tttattgtt 
      ttatacaat attaaataa ataaacagg atcagc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY