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Gene information
General information
TC011343 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid74: 1543180..1548724
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011343
    iBeetle screen iB_01842
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011343 RA
      MALYKQFQR CILHNKNPL WSPQSPFPK TFANKSGKN 
      VTNLPSMDK PVENLPTPI YASLQKEHQ TTQVTTLSN 
      GLRVASENR FGEFCTVGV VIDSGSRYE VAYPSGISH 
      FLEKLAFNS TLYYPDKDE MFNKLEKHG GICDSQASR 
      DTMIYAASA YTKGLNDVI QLLAEAALR PQITPDEID 
      GARQAISFE LETLNMRPE QETLLMDMI HAAAYRDNT 
      LGLPKLCPK KNVNRIDRE LLFTYLSQH YTPERMVVA 
      GVGVEHSKL CEAVQKHFV DKKPIWESD RTLFTPHKN 
      LGVDDSIAQ YTGGIVQEE CDIPQFASA GLPVLSHVM 
      VGLEGCSHQ DPDFIAICV LNMMLGGGG SFSAGGPGK 
      GMYTRLYTN VLNRYHWMF SATAYNHAY ADSGLLCIH 
      ASAPPNHVK EMVEVVVKE MVNMAGAVN GQELRRAKT 
      QLQSMLLMN LESRPVIFE DIGRQVLAT GHRKRPQHF 
      ITEIEKITR DDIVAVAKR LLSSQPSVA ARGDLRRMP 
      ALEFIQAGL VSDGKIQSG RKLSLFR
    CDS sequence
    • >TC011343 RA
      atggcttta tataaacag tttcagcga tgcatttta 
      cacaacaaa aacccgtta tggagcccc cagagcccc 
      ttcccaaag actttcgcc aacaaatcg ggcaagaac 
      gtcacaaat ttaccctca atggacaag ccagtcgag 
      aacctccca acccccatc tacgcctcc ctgcaaaag 
      gagcaccaa accacgcaa gtgaccacg ctctcgaac 
      gggctcagg gtggcctcc gagaaccgc ttcggcgag 
      ttctgcacc gtgggggtc gtcatagac tcaggttca 
      aggtatgag gtggcgtac ccgagcggc atttcccat 
      ttcctcgaa aaactggcc ttcaattca actttgtat 
      tatcctgac aaggacgag atgttcaac aagctggaa 
      aaacacggg ggcatttgt gacagtcag gcgtcgcgc 
      gacacgatg atttacgcg gcgtccgcg tacacgaag 
      ggcttaaac gacgtgatc cagctcctg gccgaggcg 
      gccttgcgg ccccagatc acccccgac gagatagat 
      ggcgcccga caggcgatc agtttcgag ttagaaacg 
      ctaaatatg cgccccgag caggaaacc ctactcatg 
      gacatgata cacgcggcc gcctacagg gacaacacg 
      ttggggctc ccgaagctg tgccccaag aaaaacgtc 
      aatagaatc gacagggag ctgctgttc acctacttg 
      agccagcac tacaccccc gagcgcatg gtggtggcc 
      ggggtgggg gtggagcac agtaagctg tgcgaagcc 
      gtgcagaag cactttgtc gataaaaaa cccatatgg 
      gagtcggac cgcaccctc ttcacgccg cataaaaat 
      ctaggggtt gatgacagc atagcgcag tacacgggg 
      gggatagtg caggaagag tgtgatata ccccagttc 
      gcctcagcc gggctcccc gtcctctcc cacgtgatg 
      gtgggcctc gagggctgt tcgcaccaa gaccccgac 
      ttcatcgcc atctgcgtc ctcaacatg atgctaggg 
      ggcggtggg tcattcagc gccggaggg cccggcaaa 
      ggcatgtac acgcgcctc tacaccaac gtactgaac 
      agataccac tggatgttc agcgccacc gcctacaac 
      cacgcctat gccgacagc ggccttttg tgcattcac 
      gcaagtgcg cccccaaac cacgtcaaa gaaatggtc 
      gaagtggtc gtcaaagag atggtcaac atggcgggg 
      gcggtcaat ggacaagaa ctaaggaga gcgaaaacg 
      caactccag tccatgctg ctgatgaac ttggagtcg 
      cgaccggtg atttttgag gacattggg cggcaagtg 
      ctggcgacc gggcaccgg aaacggccg caacatttc 
      attaccgaa atcgaaaaa atcactcgg gatgatatt 
      gtggccgtt gcgaaaaga ctgttaagt tcacagccg 
      tccgtggcg gcccgaggg gacctgcgc cgaatgccg 
      gccctggaa ttcatccaa gctgggctc gtcagcgac 
      ggtaaaata caaagtgga cggaagtta tcattattt 
      cgctaa
    mRNA sequence
    • >TC011343 RA
      ccaaaaatt actctgtca actgtcaaa ttgcagtct 
      catgtcaaa aacattgaa aatttaacc atttctact 
      tcatcttca accttcaga gttatggct ttatataaa 
      cagtttcag cgatgcatt ttacacaac aaaaacccg 
      ttatggagc ccccagagc cccttccca aagactttc 
      gccaacaaa tcgggcaag aacgtcaca aatttaccc 
      tcaatggac aagccagtc gagaacctc ccaaccccc 
      atctacgcc tccctgcaa aaggagcac caaaccacg 
      caagtgacc acgctctcg aacgggctc agggtggcc 
      tccgagaac cgcttcggc gagttctgc accgtgggg 
      gtcgtcata gactcaggt tcaaggtat gaggtggcg 
      tacccgagc ggcatttcc catttcctc gaaaaactg 
      gccttcaat tcaactttg tattatcct gacaaggac 
      gagatgttc aacaagctg gaaaaacac gggggcatt 
      tgtgacagt caggcgtcg cgcgacacg atgatttac 
      gcggcgtcc gcgtacacg aagggctta aacgacgtg 
      atccagctc ctggccgag gcggccttg cggccccag 
      atcaccccc gacgagata gatggcgcc cgacaggcg 
      atcagtttc gagttagaa acgctaaat atgcgcccc 
      gagcaggaa accctactc atggacatg atacacgcg 
      gccgcctac agggacaac acgttgggg ctcccgaag 
      ctgtgcccc aagaaaaac gtcaataga atcgacagg 
      gagctgctg ttcacctac ttgagccag cactacacc 
      cccgagcgc atggtggtg gccggggtg ggggtggag 
      cacagtaag ctgtgcgaa gccgtgcag aagcacttt 
      gtcgataaa aaacccata tgggagtcg gaccgcacc 
      ctcttcacg ccgcataaa aatctaggg gttgatgac 
      agcatagcg cagtacacg ggggggata gtgcaggaa 
      gagtgtgat ataccccag ttcgcctca gccgggctc 
      cccgtcctc tcccacgtg atggtgggc ctcgagggc 
      tgttcgcac caagacccc gacttcatc gccatctgc 
      gtcctcaac atgatgcta gggggcggt gggtcattc 
      agcgccgga gggcccggc aaaggcatg tacacgcgc 
      ctctacacc aacgtactg aacagatac cactggatg 
      ttcagcgcc accgcctac aaccacgcc tatgccgac 
      agcggcctt ttgtgcatt cacgcaagt gcgccccca 
      aaccacgtc aaagaaatg gtcgaagtg gtcgtcaaa 
      gagatggtc aacatggcg ggggcggtc aatggacaa 
      gaactaagg agagcgaaa acgcaactc cagtccatg 
      ctgctgatg aacttggag tcgcgaccg gtgattttt 
      gaggacatt gggcggcaa gtgctggcg accgggcac 
      cggaaacgg ccgcaacat ttcattacc gaaatcgaa 
      aaaatcact cgggatgat attgtggcc gttgcgaaa 
      agactgtta agttcacag ccgtccgtg gcggcccga 
      ggggacctg cgccgaatg ccggccctg gaattcatc 
      caagctggg ctcgtcagc gacggtaaa atacaaagt 
      ggacggaag ttatcatta tttcgctaa acattttcg 
      taaaaaatt gtctcgtgt aaatatttt tcgttaatt 
      aaggtggtt aaaacaata ggct