| General information | |||
| TC011326 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007425.3: 3472213..3494507 | |
| Sequence information | |||
| Transcripts / Proteins (4) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC011326 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01841 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
- >TC011326 RA
MSDRKAELE RKKARLQAL REEKDRRRR EKELKDVEE AANRVILGT EGDRKELDE MLSSLGVAP VSDILSSIS SANSLTPDH SHNHTPDTS LLPHSSPNN LSHAKKRAP NLSVVQVQS TNIPPKETV LYTKQTQTT ATGHERDAH ATDYYVLTY DDGQGEDEE GSLPHLGGF GSKLPPGIL PHGLPQVKE VQPAVTALE QEQAKQEVK EVRELSEEE KQMIILSED FQHFVDRAG RVMERVLCE NVDYYTDYS GGAGEEGLD EKSHQRISL NRYFFDERW SRNRCVTSM DWSPQFPEL LLASYNNNE DSPNDPDGV VLIWNTKFK KATPEYIFH CQSAVLSAK FARFHPNLI LGGTYSGQI VLWDNRVQK RTPIQRTPL SASAHTHPV YCLSVVGTQ NAHNLISIS TDGRLCSWS LDMLGQPQE TLDLHRMQS KPVAVTCLD FPHSDVNNF VVGSEEGAA YSACRHGAK AGITDTYDG HQGPITGIS VNSVQGGID FSHMFLTSS IDWTIKLWS LKDSKPIYS FEDNGDYVL DVKWSPAHP ALFAAVDGS GRLDLWNLN QDTEVPAAS VTVEGNPAL NRISWTPSG LHVTVGDNQ GKIWVYDVA ENFAHPRHD EWNKFLYTT QELRNNQVD EELEKLNYS VSNTPLSSM GSITTTPLR
- >TC011326 RB
MSDRKAELE RKKARLQAL REEKDRRRR EKELKDVEE AANRVILGT EGDRKELDE MLSSLGVAP VSDILSSIS SANSLTPDH SHNHTPDTS LLPHSSPNN LSHAKKRAP NLSVVQVQS TNIPPKETV LYTKQTQTT ATGHERDAH ATDYYGKFM ILFSIFAYS VSIIYDTRP CPFCSSRKY TEGTFSFIF LSRFFTHVL FKGHMLDFL D
- >TC011326 RC
MIILSEDFQ HFVDRAGRV MERVLCENV DYYTDYSGG AGEEGLDEK SHQRISLNR YFFDERWSR NRCVTSMDW SPQFPELLL ASYNNNEDS PNDPDGVVL IWNTKFKKA TPEYIFHCQ SAVLSAKFA RFHPNLILG GTYSGQIVL WDNRVQKRT PIQRTPLSA SAHTHPVYC LSVVGTQNA HNLISISTD GRLCSWSLD MLGQPQETL DLHRMQSKP VAVTCLDFP HSDVNNFVV GSEEGAAYS ACRHGAKAG ITDTYDGHQ GPITGISVN SVQGGIDFS HMFLTSSID WTIKLWSLK DSKPIYSFE DNGDYVLDV KWSPAHPAL FAAVDGSGR LDLWNLNQD TEVPAASVT VEGNPALNR ISWTPSGLH VTVGDNQGK IWVYDVAEN FAHPRHDEW NKFLYTTQE LRNNQVDEE LEKLNYSVS NTPLSSMGS ITTTPLR
- >TC011326 RD
MSDRKAELE RKKARLQAL REEKDRRRR EKELKDVEE AANRVILGT EGDRKELDE MLSSLGVAP VSDILSSIS SANSLTPDH SHNHTPDTS LLPHSSPNN LSHAKKRAP NLSVVQVQS TNIPPKETV LYTKQTQTT ATGHERDGK LILCLS
- >TC011326 RA
atgtcggac cgcaaagcc gaactggag aggaagaag gcccggctc caggcgctg agggaggag aaggaccgc cggcgccgg gagaaggaa ctcaaggat gtggaggag gcggccaac agggtgatt ttggggacg gaaggagac aggaaggag ttggatgaa atgctcagc tcgttgggg gttgccccc gtttcggat attttgtcg agtatttcg agcgcgaac tcgctcacg cccgaccac agccacaat cacaccccg gacacgtcc ctcctgccc cacagttca cccaacaat cttagtcat gcaaaaaaa cgcgctccg aatttgagc gtagtccag gtacagtcc accaacata ccaccgaaa gaaaccgta ctttacaca aaacaaaca caaacaacg gcaacagga cacgaacgg gacgctcat gcaaccgat tactacgtt ttgacgtat gacgatgga caaggtgaa gacgaagaa ggtagtctt ccacatttg ggcggattc ggcagcaaa ctaccaccg gggattcta ccacatggg ctcccgcag gtcaaggag gtgcagccg gccgttacg gccctggag caggaacag gccaaacag gaggtcaag gaagtccgg gaactgagc gaggaggag aagcagatg atcatcctc tctgaggac ttccagcac tttgtagat agggcggga cgagtgatg gaacgggtc ttgtgtgaa aatgtggat tattacact gattacagc ggaggagcg ggagaagaa ggattagat gagaaatcg catcagcgc atctctctc aatcgctac ttcttcgac gagcgatgg tcgcgtaat cgctgcgtt acgtcgatg gattggtcg ccacagttt cccgaatta ctgttggct tcctacaac aacaacgaa gactcgccg aacgatccc gatggcgtg gttttgatt tggaatacc aaatttaag aaggcaacg cctgaatat atttttcat tgtcaaagt gctgtgttg tcggcgaaa tttgcacga tttcatccg aatctcatc ttgggtggt acttattcg ggacagatc gtcctgtgg gataaccgg gtgcagaag cggacgccc atccagagg acgcccttg tcggcctcg gcacacacg caccctgtc tactgcttg agcgtggtg ggcacccag aacgcacac aacctgata agtatctcg actgacggt cgtctctgc tcctggtcg ttggacatg ttaggccag cctcaagag acccttgac ttgcaccga atgcaatcg aaacccgtg gctgtgaca tgtttagat tttccacat tccgacgtt aataacttt gtggtaggc agcgaagag ggggctgca tattcggcg tgtcgtcac ggcgccaag gccggaatt accgacact tacgacggc caccagggc cccatcact ggcattagc gtgaattcg gtccaaggt gggattgat ttctcacac atgttcttg acgtcgtcc atcgattgg accatcaag ttgtggagt ttgaaggac tcgaaaccg atctactca ttcgaggac aacggcgac tacgttttg gacgtgaag tggtctccg gcccatccg gcccttttc gcggccgtg gacgggagc gggcggcta gatttgtgg aacctgaac caggacacg gaagtccct gctgccagc gtgacggtc gagggaaac ccggccctg aaccggatc tcctggact ccgtcgggc ctccacgtg actgtcggc gacaaccag gggaagatt tgggtgtac gatgtggcg gaaaacttc gcacatccg agacacgac gaatggaac aagttcttg tatacgacg caagagttg cgcaacaat caagtcgat gaagaatta gaaaaatta aattattca gtctccaat actcctttg tctagtatg ggctccatt acaaccact cctctgcga taa
- >TC011326 RB
atgtcggac cgcaaagcc gaactggag aggaagaag gcccggctc caggcgctg agggaggag aaggaccgc cggcgccgg gagaaggaa ctcaaggat gtggaggag gcggccaac agggtgatt ttggggacg gaaggagac aggaaggag ttggatgaa atgctcagc tcgttgggg gttgccccc gtttcggat attttgtcg agtatttcg agcgcgaac tcgctcacg cccgaccac agccacaat cacaccccg gacacgtcc ctcctgccc cacagttca cccaacaat cttagtcat gcaaaaaaa cgcgctccg aatttgagc gtagtccag gtacagtcc accaacata ccaccgaaa gaaaccgta ctttacaca aaacaaaca caaacaacg gcaacagga cacgaacgg gacgctcat gcaaccgat tactacggt aagtttatg attttattt tcaatattt gcttatagc gtttccatt atttacgat acccgtcct tgtcctttt tgttcatct cggaagtac acagaaggc actttttcg ttcattttt ctctcgaga ttttttaca catgttttg ttcaaaggg cacatgttg gattttctg gactaa
- >TC011326 RC
atgatcatc ctctctgag gacttccag cactttgta gatagggcg ggacgagtg atggaacgg gtcttgtgt gaaaatgtg gattattac actgattac agcggagga gcgggagaa gaaggatta gatgagaaa tcgcatcag cgcatctct ctcaatcgc tacttcttc gacgagcga tggtcgcgt aatcgctgc gttacgtcg atggattgg tcgccacag tttcccgaa ttactgttg gcttcctac aacaacaac gaagactcg ccgaacgat cccgatggc gtggttttg atttggaat accaaattt aagaaggca acgcctgaa tatattttt cattgtcaa agtgctgtg ttgtcggcg aaatttgca cgatttcat ccgaatctc atcttgggt ggtacttat tcgggacag atcgtcctg tgggataac cgggtgcag aagcggacg cccatccag aggacgccc ttgtcggcc tcggcacac acgcaccct gtctactgc ttgagcgtg gtgggcacc cagaacgca cacaacctg ataagtatc tcgactgac ggtcgtctc tgctcctgg tcgttggac atgttaggc cagcctcaa gagaccctt gacttgcac cgaatgcaa tcgaaaccc gtggctgtg acatgttta gattttcca cattccgac gttaataac tttgtggta ggcagcgaa gagggggct gcatattcg gcgtgtcgt cacggcgcc aaggccgga attaccgac acttacgac ggccaccag ggccccatc actggcatt agcgtgaat tcggtccaa ggtgggatt gatttctca cacatgttc ttgacgtcg tccatcgat tggaccatc aagttgtgg agtttgaag gactcgaaa ccgatctac tcattcgag gacaacggc gactacgtt ttggacgtg aagtggtct ccggcccat ccggccctt ttcgcggcc gtggacggg agcgggcgg ctagatttg tggaacctg aaccaggac acggaagtc cctgctgcc agcgtgacg gtcgaggga aacccggcc ctgaaccgg atctcctgg actccgtcg ggcctccac gtgactgtc ggcgacaac caggggaag atttgggtg tacgatgtg gcggaaaac ttcgcacat ccgagacac gacgaatgg aacaagttc ttgtatacg acgcaagag ttgcgcaac aatcaagtc gatgaagaa ttagaaaaa ttaaattat tcagtctcc aatactcct ttgtctagt atgggctcc attacaacc actcctctg cgataa
- >TC011326 RD
atgtcggac cgcaaagcc gaactggag aggaagaag gcccggctc caggcgctg agggaggag aaggaccgc cggcgccgg gagaaggaa ctcaaggat gtggaggag gcggccaac agggtgatt ttggggacg gaaggagac aggaaggag ttggatgaa atgctcagc tcgttgggg gttgccccc gtttcggat attttgtcg agtatttcg agcgcgaac tcgctcacg cccgaccac agccacaat cacaccccg gacacgtcc ctcctgccc cacagttca cccaacaat cttagtcat gcaaaaaaa cgcgctccg aatttgagc gtagtccag gtacagtcc accaacata ccaccgaaa gaaaccgta ctttacaca aaacaaaca caaacaacg gcaacagga cacgaacgg gacggtaag ttgattttg tgtttatcg taa
- >TC011326 RA
ttgtcgtac caacctagc gaccaaaaa gtgtgacaa aatgtcatc aaattgagg aaaatcgcg gttttttcg ttaaaaatc cgccgaacc ggtttctgt aatcgatca atcacgtta ttccctccc aggaattgt gattccccc gtaaaatca gcgaactcc agtcaatat atcccgaaa tctcgcgga gaaatccct gatgactcg ccacattac gtcatcaca aaaatgaaa tggtggttg tatcggtcg ggcgttgta aattaaatc caaaggaaa cgtaaaaag tcgttcgaa aaactcatt aattccacg taaattaag tgcaatttg tcgtctcgt gtaatttgt acattcata ggatttaag ccatgtcgg accgcaaag ccgaactgg agaggaaga aggcccggc tccaggcgc tgagggagg agaaggacc gccggcgcc gggagaagg aactcaagg atgtggagg aggcggcca acagggtga ttttgggga cggaaggag acaggaagg agttggatg aaatgctca gctcgttgg gggttgccc ccgtttcgg atattttgt cgagtattt cgagcgcga actcgctca cgcccgacc acagccaca atcacaccc cggacacgt ccctcctgc cccacagtt cacccaaca atcttagtc atgcaaaaa aacgcgctc cgaatttga gcgtagtcc aggtacagt ccaccaaca taccaccga aagaaaccg tactttaca caaaacaaa cacaaacaa cggcaacag gacacgaac gggacgctc atgcaaccg attactacg ttttgacgt atgacgatg gacaaggtg aagacgaag aaggtagtc ttccacatt tgggcggat tcggcagca aactaccac cggggattc taccacatg ggctcccgc aggtcaagg aggtgcagc cggccgtta cggccctgg agcaggaac aggccaaac aggaggtca aggaagtcc gggaactga gcgaggagg agaagcaga tgatcatcc tctctgagg acttccagc actttgtag atagggcgg gacgagtga tggaacggg tcttgtgtg aaaatgtgg attattaca ctgattaca gcggaggag cgggagaag aaggattag atgagaaat cgcatcagc gcatctctc tcaatcgct acttcttcg acgagcgat ggtcgcgta atcgctgcg ttacgtcga tggattggt cgccacagt ttcccgaat tactgttgg cttcctaca acaacaacg aagactcgc cgaacgatc ccgatggcg tggttttga tttggaata ccaaattta agaaggcaa cgcctgaat atatttttc attgtcaaa gtgctgtgt tgtcggcga aatttgcac gatttcatc cgaatctca tcttgggtg gtacttatt cgggacaga tcgtcctgt gggataacc gggtgcaga agcggacgc ccatccaga ggacgccct tgtcggcct cggcacaca cgcaccctg tctactgct tgagcgtgg tgggcaccc agaacgcac acaacctga taagtatct cgactgacg gtcgtctct gctcctggt cgttggaca tgttaggcc agcctcaag agacccttg acttgcacc gaatgcaat cgaaacccg tggctgtga catgtttag attttccac attccgacg ttaataact ttgtggtag gcagcgaag agggggctg catattcgg cgtgtcgtc acggcgcca aggccggaa ttaccgaca cttacgacg gccaccagg gccccatca ctggcatta gcgtgaatt cggtccaag gtgggattg atttctcac acatgttct tgacgtcgt ccatcgatt ggaccatca agttgtgga gtttgaagg actcgaaac cgatctact cattcgagg acaacggcg actacgttt tggacgtga agtggtctc cggcccatc cggcccttt tcgcggccg tggacggga gcgggcggc tagatttgt ggaacctga accaggaca cggaagtcc ctgctgcca gcgtgacgg tcgagggaa acccggccc tgaaccgga tctcctgga ctccgtcgg gcctccacg tgactgtcg gcgacaacc aggggaaga tttgggtgt acgatgtgg cggaaaact tcgcacatc cgagacacg acgaatgga acaagttct tgtatacga cgcaagagt tgcgcaaca atcaagtcg atgaagaat tagaaaaat taaattatt cagtctcca atactcctt tgtctagta tgggctcca ttacaacca ctcctctgc gataaatag ccaatttaa tcgactagc tctaattta ttatcaagt gttataaac ggttttgat ttttgcaga tgcggtctg ggaatcgga agagtattg gtaacgaaa cgtactaaa aaacagaac ggcggattt atttttttg gccattgta tcattgctt ggatcagtg taattcagc tgtagttaa gtaccatta ttctgaata cttacttaa ataatttcg cttgtaaat agctaactt attttgttt tttttttat attaacaag taacttttt tacattgtt attttgtac catacgtgc cgttgtaaa catatttat ttgattaaa ttgcattta tcattggta ttttgaaat tcgtcaaac gagacttta aaccgaggt gtaataa
- >TC011326 RB
ttgtcgtac caacctagc gaccaaaaa gtgtgacaa aatgtcatc aaattgagg aaaatcgcg gttttttcg ttaaaaatc cgccgaacc ggtttctgt aatcgatca atcacgtta ttccctccc aggaattgt gattccccc gtaaaatca gcgaactcc agtcaatat atcccgaaa tctcgcgga gaaatccct gatgactcg ccacattac gtcatcaca aaaatgaaa tggtggttg tatcggtcg ggcgttgta aattaaatc caaaggaaa cgtaaaaag tcgttcgaa aaactcatt aattccacg taaattaag tgcaatttg tcgtctcgt gtaatttgt acattcata ggatttaag ccatgtcgg accgcaaag ccgaactgg agaggaaga aggcccggc tccaggcgc tgagggagg agaaggacc gccggcgcc gggagaagg aactcaagg atgtggagg aggcggcca acagggtga ttttgggga cggaaggag acaggaagg agttggatg aaatgctca gctcgttgg gggttgccc ccgtttcgg atattttgt cgagtattt cgagcgcga actcgctca cgcccgacc acagccaca atcacaccc cggacacgt ccctcctgc cccacagtt cacccaaca atcttagtc atgcaaaaa aacgcgctc cgaatttga gcgtagtcc aggtacagt ccaccaaca taccaccga aagaaaccg tactttaca caaaacaaa cacaaacaa cggcaacag gacacgaac gggacgctc atgcaaccg attactacg gtaagttta tgattttat tttcaatat ttgcttata gcgtttcca ttatttacg atacccgtc cttgtcctt tttgttcat ctcggaagt acacagaag gcacttttt cgttcattt ttctctcga gatttttta cacatgttt tgttcaaag ggcacatgt tggattttc tggactaac accaaagct tgtatattt gcacggtgc gggcgaaac tttgaccat tttttatat caaattaaa aaaaacatt ttgta
- >TC011326 RC
attttttaa ttcaatttt tcgttgagc aaattgtgc aatttggtt ggttttttg cgtgggcgt tgacgacta atcattttt taattattg attaagtaa ctcggacgt aataaagtt tcgcccgtc cgtcgccag cttgattaa actgtaatc tagtaacag tactcacaa ctaactaac cgcatgtta taactgcct atattgagt ttaataatg taacagttg agaggtaac cgaaaattt agttttcgg ttaatgatt gtaaaacgg ttgcagtta aagttttgt tgttagtgc agccaagtc atagacaac cttaatgta attctaata attctattc ctactgata catgtttct ttcctctta agacgaata caatctaaa tccgggttt agaatggga ggacgagtt cacaggtag gtggctttt ccgactccc atcaagatt ttaatcaat ataatagtg cactaacgt tatttttat ccatcatgt tttcatacg attaggatg tagaatgct tggtttatc gagggcgct ttttggttg cttcgttat cgattcgta gtgctagat actcaccca ctaaatgat tgagtgtaa cgtaataat caagcatgt cttttttcg tttatttat ttttctgta ttaaccgga gtggaatga aattcttgg attttttgt tctcgttcc tcctttttt cttcttcct caccgtcct agcattgcg cctcagaaa aaaaatcaa catgtaatc gatttaagt gatcgtgga ttacatatt gattttgga taaaatcgg ccgtttagt tagtttgtc gtgaatgtg ggcttatgc gtgtgggat gaaagggcg atagagcgt ttccgttat tcaccacag ttttgacgt atgacgatg gacaaggtg aagacgaag aaggtagtc ttccacatt tgggcggat tcggcagca aactaccac cggggattc taccacatg ggctcccgc aggtcaagg aggtgcagc cggccgtta cggccctgg agcaggaac aggccaaac aggaggtca aggaagtcc gggaactga gcgaggagg agaagcaga tgatcatcc tctctgagg acttccagc actttgtag atagggcgg gacgagtga tggaacggg tcttgtgtg aaaatgtgg attattaca ctgattaca gcggaggag cgggagaag aaggattag atgagaaat cgcatcagc gcatctctc tcaatcgct acttcttcg acgagcgat ggtcgcgta atcgctgcg ttacgtcga tggattggt cgccacagt ttcccgaat tactgttgg cttcctaca acaacaacg aagactcgc cgaacgatc ccgatggcg tggttttga tttggaata ccaaattta agaaggcaa cgcctgaat atatttttc attgtcaaa gtgctgtgt tgtcggcga aatttgcac gatttcatc cgaatctca tcttgggtg gtacttatt cgggacaga tcgtcctgt gggataacc gggtgcaga agcggacgc ccatccaga ggacgccct tgtcggcct cggcacaca cgcaccctg tctactgct tgagcgtgg tgggcaccc agaacgcac acaacctga taagtatct cgactgacg gtcgtctct gctcctggt cgttggaca tgttaggcc agcctcaag agacccttg acttgcacc gaatgcaat cgaaacccg tggctgtga catgtttag attttccac attccgacg ttaataact ttgtggtag gcagcgaag agggggctg catattcgg cgtgtcgtc acggcgcca aggccggaa ttaccgaca cttacgacg gccaccagg gccccatca ctggcatta gcgtgaatt cggtccaag gtgggattg atttctcac acatgttct tgacgtcgt ccatcgatt ggaccatca agttgtgga gtttgaagg actcgaaac cgatctact cattcgagg acaacggcg actacgttt tggacgtga agtggtctc cggcccatc cggcccttt tcgcggccg tggacggga gcgggcggc tagatttgt ggaacctga accaggaca cggaagtcc ctgctgcca gcgtgacgg tcgagggaa acccggccc tgaaccgga tctcctgga ctccgtcgg gcctccacg tgactgtcg gcgacaacc aggggaaga tttgggtgt acgatgtgg cggaaaact tcgcacatc cgagacacg acgaatgga acaagttct tgtatacga cgcaagagt tgcgcaaca atcaagtcg atgaagaat tagaaaaat taaattatt cagtctcca atactcctt tgtctagta tgggctcca ttacaacca ctcctctgc gataaatag ccaatttaa tcgactagc tctaattta ttatcaagt gttataaac ggttttgat ttttgcaga tgcggtctg ggaatcgga agagtattg gtaacgaaa cgtactaaa aaacagaac ggcggattt atttttttg gccattgta tcattgctt ggatcagtg taattcagc tgtagttaa gtaccatta ttctgaata cttacttaa ataatttcg cttgtaaat agctaactt attttgttt tttttttat attaacaag taacttttt tacattgtt attttgtac catacgtgc cgttgtaaa catatttat ttgattaaa ttgcattta tcattggta ttttgaaat tcgtcaaac gagacttta aaccgaggt gtaataa
- >TC011326 RD
ttgtcgtac caacctagc gaccaaaaa gtgtgacaa aatgtcatc aaattgagg aaaatcgcg gttttttcg ttaaaaatc cgccgaacc ggtttctgt aatcgatca atcacgtta ttccctccc aggaattgt gattccccc gtaaaatca gcgaactcc agtcaatat atcccgaaa tctcgcgga gaaatccct gatgactcg ccacattac gtcatcaca aaaatgaaa tggtggttg tatcggtcg ggcgttgta aattaaatc caaaggaaa cgtaaaaag tcgttcgaa aaactcatt aattccacg taaattaag tgcaatttg tcgtctcgt gtaatttgt acattcata ggatttaag ccatgtcgg accgcaaag ccgaactgg agaggaaga aggcccggc tccaggcgc tgagggagg agaaggacc gccggcgcc gggagaagg aactcaagg atgtggagg aggcggcca acagggtga ttttgggga cggaaggag acaggaagg agttggatg aaatgctca gctcgttgg gggttgccc ccgtttcgg atattttgt cgagtattt cgagcgcga actcgctca cgcccgacc acagccaca atcacaccc cggacacgt ccctcctgc cccacagtt cacccaaca atcttagtc atgcaaaaa aacgcgctc cgaatttga gcgtagtcc aggtacagt ccaccaaca taccaccga aagaaaccg tactttaca caaaacaaa cacaaacaa cggcaacag gacacgaac gggacggta agttgattt tgtgtttat cgtaatcac gagtacgca cgtgccaca ttttatatt tttttcacg gttaagttt atggaaaca gaaaaaaaa agaataaaa taaatggga tcatcattg atttgcatg aatttatct aacagcagc agcctttat taaatgttt tcttccgtt atgggaagg aaactaatt ttttttaat cgagtcagt tgtcttaat ttggctgca cgccttttt agttcgaaa ttagttact aatccggct ttggtgctt tgtatactt gatttttct ctcgctgtc ggcctgaat caaagtgaa cgtaaaaac attgttgct tccagattt ttttggatt ttcttcatg gaagaagtg tgagtaatt agaacgttg ttttgattt tcattttcg ttcaggcgt ctttttgtt tccctcctg atagaattt cattccttt ttattttat tctggtcgg gcaaaattg tgttattta ttttggtta tgtatcttc ttggtacgg aaacccttt aaagttgta tcggtggtt tccatcaac gaagattat ttaatttag acatgtgac atattgtta tatgcgttg ctaacaagg atcttcttc atcttcttc cccacttaa aggctacat ggaggactg gtggaggcc acgaaaagg tatggtgca cgtaaatgt tgacctctt taatgtact cacgaataa cagcactgt acatttatt attggtatg cacaaacta aaaaaaatt gaataaatt gtacaggag aaaattgaa aaacatgtg cacctaccc tgtcttcac gcttctttc tcctaaggt tgtggaaat ttctcatag aatacaatg gactagcgt tttaaatac aaattagtt gtcgagata gtaaatcac atgattgta cgacgtttc cttcggttt ttaatttat tattattat gcgtgtttt ttgtaattt cattgttta gttaaagtc agtttcaat atataataa tttttcttc ctttccttc ccagctcat gcaaccgat tactacggt aagtttatg attttattt tcaatattt gcttatagc gtttccatt atttacgat acccgtcct tgtcctttt tgttcatct cggaagtac acagaaggc actttttcg ttcattttt ctctcgaga ttttttaca catgttttg ttcaaaggg cacatgttg gattttctg gactaacac caaagcttg tatatttgc acggtgcgg gcgaaactt tgaccattt tttatatca aattaaaaa aaacatttt gta
