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Gene information
General information
TC011326 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3472213..3494507
Sequence information
Transcripts / Proteins (4) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011326
    iBeetle screen iB_01841

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011326 RA
      MSDRKAELE RKKARLQAL REEKDRRRR EKELKDVEE 
      AANRVILGT EGDRKELDE MLSSLGVAP VSDILSSIS 
      SANSLTPDH SHNHTPDTS LLPHSSPNN LSHAKKRAP 
      NLSVVQVQS TNIPPKETV LYTKQTQTT ATGHERDAH 
      ATDYYVLTY DDGQGEDEE GSLPHLGGF GSKLPPGIL 
      PHGLPQVKE VQPAVTALE QEQAKQEVK EVRELSEEE 
      KQMIILSED FQHFVDRAG RVMERVLCE NVDYYTDYS 
      GGAGEEGLD EKSHQRISL NRYFFDERW SRNRCVTSM 
      DWSPQFPEL LLASYNNNE DSPNDPDGV VLIWNTKFK 
      KATPEYIFH CQSAVLSAK FARFHPNLI LGGTYSGQI 
      VLWDNRVQK RTPIQRTPL SASAHTHPV YCLSVVGTQ 
      NAHNLISIS TDGRLCSWS LDMLGQPQE TLDLHRMQS 
      KPVAVTCLD FPHSDVNNF VVGSEEGAA YSACRHGAK 
      AGITDTYDG HQGPITGIS VNSVQGGID FSHMFLTSS 
      IDWTIKLWS LKDSKPIYS FEDNGDYVL DVKWSPAHP 
      ALFAAVDGS GRLDLWNLN QDTEVPAAS VTVEGNPAL 
      NRISWTPSG LHVTVGDNQ GKIWVYDVA ENFAHPRHD 
      EWNKFLYTT QELRNNQVD EELEKLNYS VSNTPLSSM 
      GSITTTPLR
    • >TC011326 RB
      MSDRKAELE RKKARLQAL REEKDRRRR EKELKDVEE 
      AANRVILGT EGDRKELDE MLSSLGVAP VSDILSSIS 
      SANSLTPDH SHNHTPDTS LLPHSSPNN LSHAKKRAP 
      NLSVVQVQS TNIPPKETV LYTKQTQTT ATGHERDAH 
      ATDYYGKFM ILFSIFAYS VSIIYDTRP CPFCSSRKY 
      TEGTFSFIF LSRFFTHVL FKGHMLDFL D
    • >TC011326 RC
      MIILSEDFQ HFVDRAGRV MERVLCENV DYYTDYSGG 
      AGEEGLDEK SHQRISLNR YFFDERWSR NRCVTSMDW 
      SPQFPELLL ASYNNNEDS PNDPDGVVL IWNTKFKKA 
      TPEYIFHCQ SAVLSAKFA RFHPNLILG GTYSGQIVL 
      WDNRVQKRT PIQRTPLSA SAHTHPVYC LSVVGTQNA 
      HNLISISTD GRLCSWSLD MLGQPQETL DLHRMQSKP 
      VAVTCLDFP HSDVNNFVV GSEEGAAYS ACRHGAKAG 
      ITDTYDGHQ GPITGISVN SVQGGIDFS HMFLTSSID 
      WTIKLWSLK DSKPIYSFE DNGDYVLDV KWSPAHPAL 
      FAAVDGSGR LDLWNLNQD TEVPAASVT VEGNPALNR 
      ISWTPSGLH VTVGDNQGK IWVYDVAEN FAHPRHDEW 
      NKFLYTTQE LRNNQVDEE LEKLNYSVS NTPLSSMGS 
      ITTTPLR
    • >TC011326 RD
      MSDRKAELE RKKARLQAL REEKDRRRR EKELKDVEE 
      AANRVILGT EGDRKELDE MLSSLGVAP VSDILSSIS 
      SANSLTPDH SHNHTPDTS LLPHSSPNN LSHAKKRAP 
      NLSVVQVQS TNIPPKETV LYTKQTQTT ATGHERDGK 
      LILCLS
    CDS sequence
    • >TC011326 RA
      atgtcggac cgcaaagcc gaactggag aggaagaag 
      gcccggctc caggcgctg agggaggag aaggaccgc 
      cggcgccgg gagaaggaa ctcaaggat gtggaggag 
      gcggccaac agggtgatt ttggggacg gaaggagac 
      aggaaggag ttggatgaa atgctcagc tcgttgggg 
      gttgccccc gtttcggat attttgtcg agtatttcg 
      agcgcgaac tcgctcacg cccgaccac agccacaat 
      cacaccccg gacacgtcc ctcctgccc cacagttca 
      cccaacaat cttagtcat gcaaaaaaa cgcgctccg 
      aatttgagc gtagtccag gtacagtcc accaacata 
      ccaccgaaa gaaaccgta ctttacaca aaacaaaca 
      caaacaacg gcaacagga cacgaacgg gacgctcat 
      gcaaccgat tactacgtt ttgacgtat gacgatgga 
      caaggtgaa gacgaagaa ggtagtctt ccacatttg 
      ggcggattc ggcagcaaa ctaccaccg gggattcta 
      ccacatggg ctcccgcag gtcaaggag gtgcagccg 
      gccgttacg gccctggag caggaacag gccaaacag 
      gaggtcaag gaagtccgg gaactgagc gaggaggag 
      aagcagatg atcatcctc tctgaggac ttccagcac 
      tttgtagat agggcggga cgagtgatg gaacgggtc 
      ttgtgtgaa aatgtggat tattacact gattacagc 
      ggaggagcg ggagaagaa ggattagat gagaaatcg 
      catcagcgc atctctctc aatcgctac ttcttcgac 
      gagcgatgg tcgcgtaat cgctgcgtt acgtcgatg 
      gattggtcg ccacagttt cccgaatta ctgttggct 
      tcctacaac aacaacgaa gactcgccg aacgatccc 
      gatggcgtg gttttgatt tggaatacc aaatttaag 
      aaggcaacg cctgaatat atttttcat tgtcaaagt 
      gctgtgttg tcggcgaaa tttgcacga tttcatccg 
      aatctcatc ttgggtggt acttattcg ggacagatc 
      gtcctgtgg gataaccgg gtgcagaag cggacgccc 
      atccagagg acgcccttg tcggcctcg gcacacacg 
      caccctgtc tactgcttg agcgtggtg ggcacccag 
      aacgcacac aacctgata agtatctcg actgacggt 
      cgtctctgc tcctggtcg ttggacatg ttaggccag 
      cctcaagag acccttgac ttgcaccga atgcaatcg 
      aaacccgtg gctgtgaca tgtttagat tttccacat 
      tccgacgtt aataacttt gtggtaggc agcgaagag 
      ggggctgca tattcggcg tgtcgtcac ggcgccaag 
      gccggaatt accgacact tacgacggc caccagggc 
      cccatcact ggcattagc gtgaattcg gtccaaggt 
      gggattgat ttctcacac atgttcttg acgtcgtcc 
      atcgattgg accatcaag ttgtggagt ttgaaggac 
      tcgaaaccg atctactca ttcgaggac aacggcgac 
      tacgttttg gacgtgaag tggtctccg gcccatccg 
      gcccttttc gcggccgtg gacgggagc gggcggcta 
      gatttgtgg aacctgaac caggacacg gaagtccct 
      gctgccagc gtgacggtc gagggaaac ccggccctg 
      aaccggatc tcctggact ccgtcgggc ctccacgtg 
      actgtcggc gacaaccag gggaagatt tgggtgtac 
      gatgtggcg gaaaacttc gcacatccg agacacgac 
      gaatggaac aagttcttg tatacgacg caagagttg 
      cgcaacaat caagtcgat gaagaatta gaaaaatta 
      aattattca gtctccaat actcctttg tctagtatg 
      ggctccatt acaaccact cctctgcga taa
    • >TC011326 RB
      atgtcggac cgcaaagcc gaactggag aggaagaag 
      gcccggctc caggcgctg agggaggag aaggaccgc 
      cggcgccgg gagaaggaa ctcaaggat gtggaggag 
      gcggccaac agggtgatt ttggggacg gaaggagac 
      aggaaggag ttggatgaa atgctcagc tcgttgggg 
      gttgccccc gtttcggat attttgtcg agtatttcg 
      agcgcgaac tcgctcacg cccgaccac agccacaat 
      cacaccccg gacacgtcc ctcctgccc cacagttca 
      cccaacaat cttagtcat gcaaaaaaa cgcgctccg 
      aatttgagc gtagtccag gtacagtcc accaacata 
      ccaccgaaa gaaaccgta ctttacaca aaacaaaca 
      caaacaacg gcaacagga cacgaacgg gacgctcat 
      gcaaccgat tactacggt aagtttatg attttattt 
      tcaatattt gcttatagc gtttccatt atttacgat 
      acccgtcct tgtcctttt tgttcatct cggaagtac 
      acagaaggc actttttcg ttcattttt ctctcgaga 
      ttttttaca catgttttg ttcaaaggg cacatgttg 
      gattttctg gactaa
    • >TC011326 RC
      atgatcatc ctctctgag gacttccag cactttgta 
      gatagggcg ggacgagtg atggaacgg gtcttgtgt 
      gaaaatgtg gattattac actgattac agcggagga 
      gcgggagaa gaaggatta gatgagaaa tcgcatcag 
      cgcatctct ctcaatcgc tacttcttc gacgagcga 
      tggtcgcgt aatcgctgc gttacgtcg atggattgg 
      tcgccacag tttcccgaa ttactgttg gcttcctac 
      aacaacaac gaagactcg ccgaacgat cccgatggc 
      gtggttttg atttggaat accaaattt aagaaggca 
      acgcctgaa tatattttt cattgtcaa agtgctgtg 
      ttgtcggcg aaatttgca cgatttcat ccgaatctc 
      atcttgggt ggtacttat tcgggacag atcgtcctg 
      tgggataac cgggtgcag aagcggacg cccatccag 
      aggacgccc ttgtcggcc tcggcacac acgcaccct 
      gtctactgc ttgagcgtg gtgggcacc cagaacgca 
      cacaacctg ataagtatc tcgactgac ggtcgtctc 
      tgctcctgg tcgttggac atgttaggc cagcctcaa 
      gagaccctt gacttgcac cgaatgcaa tcgaaaccc 
      gtggctgtg acatgttta gattttcca cattccgac 
      gttaataac tttgtggta ggcagcgaa gagggggct 
      gcatattcg gcgtgtcgt cacggcgcc aaggccgga 
      attaccgac acttacgac ggccaccag ggccccatc 
      actggcatt agcgtgaat tcggtccaa ggtgggatt 
      gatttctca cacatgttc ttgacgtcg tccatcgat 
      tggaccatc aagttgtgg agtttgaag gactcgaaa 
      ccgatctac tcattcgag gacaacggc gactacgtt 
      ttggacgtg aagtggtct ccggcccat ccggccctt 
      ttcgcggcc gtggacggg agcgggcgg ctagatttg 
      tggaacctg aaccaggac acggaagtc cctgctgcc 
      agcgtgacg gtcgaggga aacccggcc ctgaaccgg 
      atctcctgg actccgtcg ggcctccac gtgactgtc 
      ggcgacaac caggggaag atttgggtg tacgatgtg 
      gcggaaaac ttcgcacat ccgagacac gacgaatgg 
      aacaagttc ttgtatacg acgcaagag ttgcgcaac 
      aatcaagtc gatgaagaa ttagaaaaa ttaaattat 
      tcagtctcc aatactcct ttgtctagt atgggctcc 
      attacaacc actcctctg cgataa
    • >TC011326 RD
      atgtcggac cgcaaagcc gaactggag aggaagaag 
      gcccggctc caggcgctg agggaggag aaggaccgc 
      cggcgccgg gagaaggaa ctcaaggat gtggaggag 
      gcggccaac agggtgatt ttggggacg gaaggagac 
      aggaaggag ttggatgaa atgctcagc tcgttgggg 
      gttgccccc gtttcggat attttgtcg agtatttcg 
      agcgcgaac tcgctcacg cccgaccac agccacaat 
      cacaccccg gacacgtcc ctcctgccc cacagttca 
      cccaacaat cttagtcat gcaaaaaaa cgcgctccg 
      aatttgagc gtagtccag gtacagtcc accaacata 
      ccaccgaaa gaaaccgta ctttacaca aaacaaaca 
      caaacaacg gcaacagga cacgaacgg gacggtaag 
      ttgattttg tgtttatcg taa
    mRNA sequence
    • >TC011326 RA
      ttgtcgtac caacctagc gaccaaaaa gtgtgacaa 
      aatgtcatc aaattgagg aaaatcgcg gttttttcg 
      ttaaaaatc cgccgaacc ggtttctgt aatcgatca 
      atcacgtta ttccctccc aggaattgt gattccccc 
      gtaaaatca gcgaactcc agtcaatat atcccgaaa 
      tctcgcgga gaaatccct gatgactcg ccacattac 
      gtcatcaca aaaatgaaa tggtggttg tatcggtcg 
      ggcgttgta aattaaatc caaaggaaa cgtaaaaag 
      tcgttcgaa aaactcatt aattccacg taaattaag 
      tgcaatttg tcgtctcgt gtaatttgt acattcata 
      ggatttaag ccatgtcgg accgcaaag ccgaactgg 
      agaggaaga aggcccggc tccaggcgc tgagggagg 
      agaaggacc gccggcgcc gggagaagg aactcaagg 
      atgtggagg aggcggcca acagggtga ttttgggga 
      cggaaggag acaggaagg agttggatg aaatgctca 
      gctcgttgg gggttgccc ccgtttcgg atattttgt 
      cgagtattt cgagcgcga actcgctca cgcccgacc 
      acagccaca atcacaccc cggacacgt ccctcctgc 
      cccacagtt cacccaaca atcttagtc atgcaaaaa 
      aacgcgctc cgaatttga gcgtagtcc aggtacagt 
      ccaccaaca taccaccga aagaaaccg tactttaca 
      caaaacaaa cacaaacaa cggcaacag gacacgaac 
      gggacgctc atgcaaccg attactacg ttttgacgt 
      atgacgatg gacaaggtg aagacgaag aaggtagtc 
      ttccacatt tgggcggat tcggcagca aactaccac 
      cggggattc taccacatg ggctcccgc aggtcaagg 
      aggtgcagc cggccgtta cggccctgg agcaggaac 
      aggccaaac aggaggtca aggaagtcc gggaactga 
      gcgaggagg agaagcaga tgatcatcc tctctgagg 
      acttccagc actttgtag atagggcgg gacgagtga 
      tggaacggg tcttgtgtg aaaatgtgg attattaca 
      ctgattaca gcggaggag cgggagaag aaggattag 
      atgagaaat cgcatcagc gcatctctc tcaatcgct 
      acttcttcg acgagcgat ggtcgcgta atcgctgcg 
      ttacgtcga tggattggt cgccacagt ttcccgaat 
      tactgttgg cttcctaca acaacaacg aagactcgc 
      cgaacgatc ccgatggcg tggttttga tttggaata 
      ccaaattta agaaggcaa cgcctgaat atatttttc 
      attgtcaaa gtgctgtgt tgtcggcga aatttgcac 
      gatttcatc cgaatctca tcttgggtg gtacttatt 
      cgggacaga tcgtcctgt gggataacc gggtgcaga 
      agcggacgc ccatccaga ggacgccct tgtcggcct 
      cggcacaca cgcaccctg tctactgct tgagcgtgg 
      tgggcaccc agaacgcac acaacctga taagtatct 
      cgactgacg gtcgtctct gctcctggt cgttggaca 
      tgttaggcc agcctcaag agacccttg acttgcacc 
      gaatgcaat cgaaacccg tggctgtga catgtttag 
      attttccac attccgacg ttaataact ttgtggtag 
      gcagcgaag agggggctg catattcgg cgtgtcgtc 
      acggcgcca aggccggaa ttaccgaca cttacgacg 
      gccaccagg gccccatca ctggcatta gcgtgaatt 
      cggtccaag gtgggattg atttctcac acatgttct 
      tgacgtcgt ccatcgatt ggaccatca agttgtgga 
      gtttgaagg actcgaaac cgatctact cattcgagg 
      acaacggcg actacgttt tggacgtga agtggtctc 
      cggcccatc cggcccttt tcgcggccg tggacggga 
      gcgggcggc tagatttgt ggaacctga accaggaca 
      cggaagtcc ctgctgcca gcgtgacgg tcgagggaa 
      acccggccc tgaaccgga tctcctgga ctccgtcgg 
      gcctccacg tgactgtcg gcgacaacc aggggaaga 
      tttgggtgt acgatgtgg cggaaaact tcgcacatc 
      cgagacacg acgaatgga acaagttct tgtatacga 
      cgcaagagt tgcgcaaca atcaagtcg atgaagaat 
      tagaaaaat taaattatt cagtctcca atactcctt 
      tgtctagta tgggctcca ttacaacca ctcctctgc 
      gataaatag ccaatttaa tcgactagc tctaattta 
      ttatcaagt gttataaac ggttttgat ttttgcaga 
      tgcggtctg ggaatcgga agagtattg gtaacgaaa 
      cgtactaaa aaacagaac ggcggattt atttttttg 
      gccattgta tcattgctt ggatcagtg taattcagc 
      tgtagttaa gtaccatta ttctgaata cttacttaa 
      ataatttcg cttgtaaat agctaactt attttgttt 
      tttttttat attaacaag taacttttt tacattgtt 
      attttgtac catacgtgc cgttgtaaa catatttat 
      ttgattaaa ttgcattta tcattggta ttttgaaat 
      tcgtcaaac gagacttta aaccgaggt gtaataa
    • >TC011326 RB
      ttgtcgtac caacctagc gaccaaaaa gtgtgacaa 
      aatgtcatc aaattgagg aaaatcgcg gttttttcg 
      ttaaaaatc cgccgaacc ggtttctgt aatcgatca 
      atcacgtta ttccctccc aggaattgt gattccccc 
      gtaaaatca gcgaactcc agtcaatat atcccgaaa 
      tctcgcgga gaaatccct gatgactcg ccacattac 
      gtcatcaca aaaatgaaa tggtggttg tatcggtcg 
      ggcgttgta aattaaatc caaaggaaa cgtaaaaag 
      tcgttcgaa aaactcatt aattccacg taaattaag 
      tgcaatttg tcgtctcgt gtaatttgt acattcata 
      ggatttaag ccatgtcgg accgcaaag ccgaactgg 
      agaggaaga aggcccggc tccaggcgc tgagggagg 
      agaaggacc gccggcgcc gggagaagg aactcaagg 
      atgtggagg aggcggcca acagggtga ttttgggga 
      cggaaggag acaggaagg agttggatg aaatgctca 
      gctcgttgg gggttgccc ccgtttcgg atattttgt 
      cgagtattt cgagcgcga actcgctca cgcccgacc 
      acagccaca atcacaccc cggacacgt ccctcctgc 
      cccacagtt cacccaaca atcttagtc atgcaaaaa 
      aacgcgctc cgaatttga gcgtagtcc aggtacagt 
      ccaccaaca taccaccga aagaaaccg tactttaca 
      caaaacaaa cacaaacaa cggcaacag gacacgaac 
      gggacgctc atgcaaccg attactacg gtaagttta 
      tgattttat tttcaatat ttgcttata gcgtttcca 
      ttatttacg atacccgtc cttgtcctt tttgttcat 
      ctcggaagt acacagaag gcacttttt cgttcattt 
      ttctctcga gatttttta cacatgttt tgttcaaag 
      ggcacatgt tggattttc tggactaac accaaagct 
      tgtatattt gcacggtgc gggcgaaac tttgaccat 
      tttttatat caaattaaa aaaaacatt ttgta
    • >TC011326 RC
      attttttaa ttcaatttt tcgttgagc aaattgtgc 
      aatttggtt ggttttttg cgtgggcgt tgacgacta 
      atcattttt taattattg attaagtaa ctcggacgt 
      aataaagtt tcgcccgtc cgtcgccag cttgattaa 
      actgtaatc tagtaacag tactcacaa ctaactaac 
      cgcatgtta taactgcct atattgagt ttaataatg 
      taacagttg agaggtaac cgaaaattt agttttcgg 
      ttaatgatt gtaaaacgg ttgcagtta aagttttgt 
      tgttagtgc agccaagtc atagacaac cttaatgta 
      attctaata attctattc ctactgata catgtttct 
      ttcctctta agacgaata caatctaaa tccgggttt 
      agaatggga ggacgagtt cacaggtag gtggctttt 
      ccgactccc atcaagatt ttaatcaat ataatagtg 
      cactaacgt tatttttat ccatcatgt tttcatacg 
      attaggatg tagaatgct tggtttatc gagggcgct 
      ttttggttg cttcgttat cgattcgta gtgctagat 
      actcaccca ctaaatgat tgagtgtaa cgtaataat 
      caagcatgt cttttttcg tttatttat ttttctgta 
      ttaaccgga gtggaatga aattcttgg attttttgt 
      tctcgttcc tcctttttt cttcttcct caccgtcct 
      agcattgcg cctcagaaa aaaaatcaa catgtaatc 
      gatttaagt gatcgtgga ttacatatt gattttgga 
      taaaatcgg ccgtttagt tagtttgtc gtgaatgtg 
      ggcttatgc gtgtgggat gaaagggcg atagagcgt 
      ttccgttat tcaccacag ttttgacgt atgacgatg 
      gacaaggtg aagacgaag aaggtagtc ttccacatt 
      tgggcggat tcggcagca aactaccac cggggattc 
      taccacatg ggctcccgc aggtcaagg aggtgcagc 
      cggccgtta cggccctgg agcaggaac aggccaaac 
      aggaggtca aggaagtcc gggaactga gcgaggagg 
      agaagcaga tgatcatcc tctctgagg acttccagc 
      actttgtag atagggcgg gacgagtga tggaacggg 
      tcttgtgtg aaaatgtgg attattaca ctgattaca 
      gcggaggag cgggagaag aaggattag atgagaaat 
      cgcatcagc gcatctctc tcaatcgct acttcttcg 
      acgagcgat ggtcgcgta atcgctgcg ttacgtcga 
      tggattggt cgccacagt ttcccgaat tactgttgg 
      cttcctaca acaacaacg aagactcgc cgaacgatc 
      ccgatggcg tggttttga tttggaata ccaaattta 
      agaaggcaa cgcctgaat atatttttc attgtcaaa 
      gtgctgtgt tgtcggcga aatttgcac gatttcatc 
      cgaatctca tcttgggtg gtacttatt cgggacaga 
      tcgtcctgt gggataacc gggtgcaga agcggacgc 
      ccatccaga ggacgccct tgtcggcct cggcacaca 
      cgcaccctg tctactgct tgagcgtgg tgggcaccc 
      agaacgcac acaacctga taagtatct cgactgacg 
      gtcgtctct gctcctggt cgttggaca tgttaggcc 
      agcctcaag agacccttg acttgcacc gaatgcaat 
      cgaaacccg tggctgtga catgtttag attttccac 
      attccgacg ttaataact ttgtggtag gcagcgaag 
      agggggctg catattcgg cgtgtcgtc acggcgcca 
      aggccggaa ttaccgaca cttacgacg gccaccagg 
      gccccatca ctggcatta gcgtgaatt cggtccaag 
      gtgggattg atttctcac acatgttct tgacgtcgt 
      ccatcgatt ggaccatca agttgtgga gtttgaagg 
      actcgaaac cgatctact cattcgagg acaacggcg 
      actacgttt tggacgtga agtggtctc cggcccatc 
      cggcccttt tcgcggccg tggacggga gcgggcggc 
      tagatttgt ggaacctga accaggaca cggaagtcc 
      ctgctgcca gcgtgacgg tcgagggaa acccggccc 
      tgaaccgga tctcctgga ctccgtcgg gcctccacg 
      tgactgtcg gcgacaacc aggggaaga tttgggtgt 
      acgatgtgg cggaaaact tcgcacatc cgagacacg 
      acgaatgga acaagttct tgtatacga cgcaagagt 
      tgcgcaaca atcaagtcg atgaagaat tagaaaaat 
      taaattatt cagtctcca atactcctt tgtctagta 
      tgggctcca ttacaacca ctcctctgc gataaatag 
      ccaatttaa tcgactagc tctaattta ttatcaagt 
      gttataaac ggttttgat ttttgcaga tgcggtctg 
      ggaatcgga agagtattg gtaacgaaa cgtactaaa 
      aaacagaac ggcggattt atttttttg gccattgta 
      tcattgctt ggatcagtg taattcagc tgtagttaa 
      gtaccatta ttctgaata cttacttaa ataatttcg 
      cttgtaaat agctaactt attttgttt tttttttat 
      attaacaag taacttttt tacattgtt attttgtac 
      catacgtgc cgttgtaaa catatttat ttgattaaa 
      ttgcattta tcattggta ttttgaaat tcgtcaaac 
      gagacttta aaccgaggt gtaataa
    • >TC011326 RD
      ttgtcgtac caacctagc gaccaaaaa gtgtgacaa 
      aatgtcatc aaattgagg aaaatcgcg gttttttcg 
      ttaaaaatc cgccgaacc ggtttctgt aatcgatca 
      atcacgtta ttccctccc aggaattgt gattccccc 
      gtaaaatca gcgaactcc agtcaatat atcccgaaa 
      tctcgcgga gaaatccct gatgactcg ccacattac 
      gtcatcaca aaaatgaaa tggtggttg tatcggtcg 
      ggcgttgta aattaaatc caaaggaaa cgtaaaaag 
      tcgttcgaa aaactcatt aattccacg taaattaag 
      tgcaatttg tcgtctcgt gtaatttgt acattcata 
      ggatttaag ccatgtcgg accgcaaag ccgaactgg 
      agaggaaga aggcccggc tccaggcgc tgagggagg 
      agaaggacc gccggcgcc gggagaagg aactcaagg 
      atgtggagg aggcggcca acagggtga ttttgggga 
      cggaaggag acaggaagg agttggatg aaatgctca 
      gctcgttgg gggttgccc ccgtttcgg atattttgt 
      cgagtattt cgagcgcga actcgctca cgcccgacc 
      acagccaca atcacaccc cggacacgt ccctcctgc 
      cccacagtt cacccaaca atcttagtc atgcaaaaa 
      aacgcgctc cgaatttga gcgtagtcc aggtacagt 
      ccaccaaca taccaccga aagaaaccg tactttaca 
      caaaacaaa cacaaacaa cggcaacag gacacgaac 
      gggacggta agttgattt tgtgtttat cgtaatcac 
      gagtacgca cgtgccaca ttttatatt tttttcacg 
      gttaagttt atggaaaca gaaaaaaaa agaataaaa 
      taaatggga tcatcattg atttgcatg aatttatct 
      aacagcagc agcctttat taaatgttt tcttccgtt 
      atgggaagg aaactaatt ttttttaat cgagtcagt 
      tgtcttaat ttggctgca cgccttttt agttcgaaa 
      ttagttact aatccggct ttggtgctt tgtatactt 
      gatttttct ctcgctgtc ggcctgaat caaagtgaa 
      cgtaaaaac attgttgct tccagattt ttttggatt 
      ttcttcatg gaagaagtg tgagtaatt agaacgttg 
      ttttgattt tcattttcg ttcaggcgt ctttttgtt 
      tccctcctg atagaattt cattccttt ttattttat 
      tctggtcgg gcaaaattg tgttattta ttttggtta 
      tgtatcttc ttggtacgg aaacccttt aaagttgta 
      tcggtggtt tccatcaac gaagattat ttaatttag 
      acatgtgac atattgtta tatgcgttg ctaacaagg 
      atcttcttc atcttcttc cccacttaa aggctacat 
      ggaggactg gtggaggcc acgaaaagg tatggtgca 
      cgtaaatgt tgacctctt taatgtact cacgaataa 
      cagcactgt acatttatt attggtatg cacaaacta 
      aaaaaaatt gaataaatt gtacaggag aaaattgaa 
      aaacatgtg cacctaccc tgtcttcac gcttctttc 
      tcctaaggt tgtggaaat ttctcatag aatacaatg 
      gactagcgt tttaaatac aaattagtt gtcgagata 
      gtaaatcac atgattgta cgacgtttc cttcggttt 
      ttaatttat tattattat gcgtgtttt ttgtaattt 
      cattgttta gttaaagtc agtttcaat atataataa 
      tttttcttc ctttccttc ccagctcat gcaaccgat 
      tactacggt aagtttatg attttattt tcaatattt 
      gcttatagc gtttccatt atttacgat acccgtcct 
      tgtcctttt tgttcatct cggaagtac acagaaggc 
      actttttcg ttcattttt ctctcgaga ttttttaca 
      catgttttg ttcaaaggg cacatgttg gattttctg 
      gactaacac caaagcttg tatatttgc acggtgcgg 
      gcgaaactt tgaccattt tttatatca aattaaaaa 
      aaacatttt gta
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