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Gene information
General information
TC011321 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid74: 958319..965226
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011321
    iBeetle screen iB_01840
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition >5 number 2nd lateral oviduct: number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs random sometimesshape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection


    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 9


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011321 RA
      MANSLLLQA GLLICSICA VRSEVFTAL ADLEDLLET 
      EALLINTLE NYIKAEEEK LGYLKRYAA IYKNQHDKA 
      SENVQVYVA NPINAYTLV KRLTADWRE VENLINSDL 
      SLDCLANIT HIKETMKFP TDEDLNGAA VALTRLQDT 
      YRLDTSSLA RGELNGVKY SSELSAADC FELGRQSYN 
      NEDYYHTQL WMREADARL KREANETVD RSDILEYLA 
      FSTYKQGNI PLALDLTNK LLEILPSHP RALGNKIYY 
      EDELQKSVN TKKKGDDGG EPEGESKPY VDPYGREFY 
      EQLCRGEIS LPVEKASKL KCFYLSRNQ PFLKIAPFK 
      VEEAHHRPD IFIFRDVLA DSEIATIKR MAQPRFKRA 
      TVQNTDTGE LEIAQYRIS KSAWLKEEE HKHIADVSQ 
      RVSDMTGLT MSTAEELQV VNYGIGGHY EPHFDFARR 
      DERNAFKSL GTGNRIATV LFYMSDVEQ GGATVFPSI 
      QVSLWPQKG SAAFWYNLH PSGDGDKMT RHAACPVLT 
      GSKWVSNKW IHERGQEFR RPCTLERPS EDLVA
    CDS sequence
    • >TC011321 RA
      atggcaaat tctttgttg ttgcaagcg ggtttattg 
      atttgctcg atttgtgca gttaggagt gaggttttc 
      actgcactt gctgattta gaggatcta ctagagacc 
      gaagcgcta ctaatcaac actctcgaa aattatatt 
      aaagcggaa gaagaaaaa ttagggtat ttgaagaga 
      tatgcggca atttacaaa aatcagcac gacaaagcg 
      tcagaaaac gtgcaagtg tacgtcgcc aatccgatt 
      aatgcttac actcttgtt aaaagactg acagcggat 
      tggcgagaa gtggaaaat ttgatcaac agtgatctg 
      agtttagat tgtttagcg aatataact catattaag 
      gaaactatg aaatttccg actgacgaa gaccttaac 
      ggggctgcc gtagccttg actcgcctc caggacacc 
      tacagactt gacacctcg tcgttggct cgcggcgaa 
      ctaaacgga gtgaaatac agctccgaa ctgagcgct 
      gctgattgt ttcgaatta ggcagacaa tcctacaac 
      aatgaggat tattatcac acccaattg tggatgcga 
      gaagccgac gctcggctc aagcgagag gctaatgaa 
      acggtagac aggagcgat atattggaa tacttggct 
      ttttcgacg tacaaacaa ggtaacatc cctctagca 
      ctggatctc acaaacaaa ctcctcgaa atactccca 
      agtcatcca cgcgcactt ggaaacaag atctactac 
      gaggatgag ttacagaaa tccgtaaat acgaagaaa 
      aagggagat gatggaggg gagcctgaa ggggaatct 
      aaaccatac gttgatcct tacggacgg gagttttac 
      gaacagttg tgtcggggg gaaatttcc ctcccggtg 
      gagaaagcc tccaaactg aagtgtttt tacctcagt 
      aggaaccag ccgttcctt aaaattgct cccttcaaa 
      gttgaagaa gctcaccac aggcctgat attttcatt 
      tttcgggat gtgttggcc gatagcgag attgcaacg 
      atcaaacga atggcacaa cctaggttt aagagagcc 
      accgttcaa aacactgac actggcgaa ctagagatc 
      gcccaatac agaatcagc aaatcagcc tggctcaag 
      gaggaagaa cacaaacac atcgctgat gtttcccaa 
      agagtcagc gatatgaca gggctgaca atgtcaaca 
      gctgaggaa ctccaagtc gtcaattac ggaattggc 
      ggccattac gaaccccat tttgatttt gctcggcga 
      gacgagaga aacgccttc aaaagcctg ggaactggc 
      aacagaatc gcaaccgtg ctcttctac atgagcgac 
      gtggagcaa ggcggcgcc acggttttc cccagcatc 
      caggtctct ttgtggccc caaaaaggc tcagctgcc 
      ttctggtac aaccttcac cctagtggt gacggcgat 
      aaaatgaca aggcatgcg gcatgtccg gttctaacc 
      ggctccaaa tgggtttcg aataagtgg atccatgag 
      cgagggcaa gaatttcgg aggccttgt actttggaa 
      agacccagt gaggaccta gtggcgtga
    mRNA sequence
    • >TC011321 RA
      gtccaaaac gaattagat gattagttg tatttattt 
      attcactat tattgtcta tattttgta atacaaggc 
      gaacacaaa atggcaaat tctttgttg ttgcaagcg 
      ggtttattg atttgctcg atttgtgca gttaggagt 
      gaggttttc actgcactt gctgattta gaggatcta 
      ctagagacc gaagcgcta ctaatcaac actctcgaa 
      aattatatt aaagcggaa gaagaaaaa ttagggtat 
      ttgaagaga tatgcggca atttacaaa aatcagcac 
      gacaaagcg tcagaaaac gtgcaagtg tacgtcgcc 
      aatccgatt aatgcttac actcttgtt aaaagactg 
      acagcggat tggcgagaa gtggaaaat ttgatcaac 
      agtgatctg agtttagat tgtttagcg aatataact 
      catattaag gaaactatg aaatttccg actgacgaa 
      gaccttaac ggggctgcc gtagccttg actcgcctc 
      caggacacc tacagactt gacacctcg tcgttggct 
      cgcggcgaa ctaaacgga gtgaaatac agctccgaa 
      ctgagcgct gctgattgt ttcgaatta ggcagacaa 
      tcctacaac aatgaggat tattatcac acccaattg 
      tggatgcga gaagccgac gctcggctc aagcgagag 
      gctaatgaa acggtagac aggagcgat atattggaa 
      tacttggct ttttcgacg tacaaacaa ggtaacatc 
      cctctagca ctggatctc acaaacaaa ctcctcgaa 
      atactccca agtcatcca cgcgcactt ggaaacaag 
      atctactac gaggatgag ttacagaaa tccgtaaat 
      acgaagaaa aagggagat gatggaggg gagcctgaa 
      ggggaatct aaaccatac gttgatcct tacggacgg 
      gagttttac gaacagttg tgtcggggg gaaatttcc 
      ctcccggtg gagaaagcc tccaaactg aagtgtttt 
      tacctcagt aggaaccag ccgttcctt aaaattgct 
      cccttcaaa gttgaagaa gctcaccac aggcctgat 
      attttcatt tttcgggat gtgttggcc gatagcgag 
      attgcaacg atcaaacga atggcacaa cctaggttt 
      aagagagcc accgttcaa aacactgac actggcgaa 
      ctagagatc gcccaatac agaatcagc aaatcagcc 
      tggctcaag gaggaagaa cacaaacac atcgctgat 
      gtttcccaa agagtcagc gatatgaca gggctgaca 
      atgtcaaca gctgaggaa ctccaagtc gtcaattac 
      ggaattggc ggccattac gaaccccat tttgatttt 
      gctcggcga gacgagaga aacgccttc aaaagcctg 
      ggaactggc aacagaatc gcaaccgtg ctcttctac 
      atgagcgac gtggagcaa ggcggcgcc acggttttc 
      cccagcatc caggtctct ttgtggccc caaaaaggc 
      tcagctgcc ttctggtac aaccttcac cctagtggt 
      gacggcgat aaaatgaca aggcatgcg gcatgtccg 
      gttctaacc ggctccaaa tgggtttcg aataagtgg 
      atccatgag cgagggcaa gaatttcgg aggccttgt 
      actttggaa agacccagt gaggaccta gtggcgtga 
      ctgaagact gttcctaaa tagttttac tgtatttgc 
      tttaaacaa agattagct ttaatttat tttgacgtg 
      tactcatat tagtgaata aagccgatt tcatact