| General information | |||
| TC031364
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 19310273..19318234 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC031364 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01839 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 4 (11 dpi)
pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 4 (11 dpi)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
- >TC031364 RA
MPNLLERAE DIDFVTKTA IERDPNKRR VKPVETQAS ILDFDLEEE SSDDSDFRI EDHPEVSDD DDSGNSASG GNDSSGEEE SEGSDLDEL KNLNLPNGD GLSIADVIQ QANLQLSSF NEKDAQILI CCGCLGDQS DGVNEIVEC DSCGVTVHE ACYGVADSA SLSSTDSLS PTAPWFCEA CKAGVMNPV CELCPNSGG IFKETDVGK WIHLVCALY VPGVAFGEV DKLTSVTLF EMPYSKWGA KSCSLCQDE RFARTGVCI GCDAGMCKT YFHVTCAQR EGFLSEAHS EEVDQADPF YAHCKLHSD KTLVKRRRK NYLALQLRT HYRKLQFEK EGHSETPEQ VRIQRKLEK QKLHFLNHK ALKPPPWVP TQKMPRLLT TSASACRKL LRKAELMGI NTMVLEMQE AQAAALVDV RKKWHIPPA FTVEFIAYY LDRNDRLKG MKQTLEKLL EDNRDLLEE QQLLRTKYD EILKKSADE TKKNIDLKQ TIHKYHSAI LSACPTKNL PNIEDLGKP QVSRTNQPM MVPTAAALK MGVGFPLNN IIPAGRTDR VLSSHAKQD SLMLNECGI CRQKRDQHL LAKCDTCHL YYHLNCLNP PLTRLPKKS KLYGWQCSE CDKTSDSEV EEVKTPRRS KSRYSREAR SDPEVSTPK LKIKPVEPV SEPTPTPTP TPEIVPIQT SHSEVVSEM NGFQSLDGS SKIHVSKSG KKRRREKHK NRYSPDLGA PSKEHKRKR KKKSLDFDD HMPHPRITI KIKPIPLPA GEVASESNP QCFYVPNEN NDNAPPPIS LKVISLPEE MSPKDIEKN PVSDICDVC HTATVASTS VRCDDCQKT YHFTCLDPP LKKSPKKRG YSWHCADCD PTGSE
- >TC031364 RB
MPNLLERAE DIDFVTKTA SKSPSTPSF SKIHYKLSR DKKRLNLST IGDLGPEMS SLERDPNKR RVKPVETQA SILDFDLEE ESSDDSDFR IEDHPEVSD DDDSGNSAS GGNDSSGEE ESEGSDLDE LKNLNLPNG DGLSIADVI QQANLQLSS FNEKDAQIL ICCGCLGDQ SDGVNEIVE CDSCGVTVH EACYGVADS ASLSSTDSL SPTAPWFCE ACKAGVMNP VCELCPNSG GIFKETDVG KWIHLVCAL YVPGVAFGE VDKLTSVTL FEMPYSKWG AKSCSLCQD ERFARTGVC IGCDAGMCK TYFHVTCAQ REGFLSEAH SEEVDQADP FYAHCKLHS DKTLVKRRR KNYLALQLR THYRKLQFE KEGHSETPE QVRIQRKLE KQKLHFLNH KALKPPPWV PTQKMPRLL TTSASACRK LLRKAELMG INTMVLEMQ EAQAAALVD VRKKWHIPP AFTVEFIAY YLDRNDRLK GMKQTLEKL LEDNRDLLE EQQLLRTKY DEILKKSAD ETKKNIDLK QTIHKYHSA ILSACPTKN LPNIEDLGK PQVSRTNQP MMVPTAAAL KMGVGFPLN NIIPAGRTD RVLSSHAKQ DSLMLNECG ICRQKRDQH LLAKCDTCH LYYHLNCLN PPLTRLPKK SKLYGWQCS ECDKTSDSE VEEVKTPRR SKSRYSREA RSDPEVSTP KLKIKPVEP VSEPTPTPT PTPEIVPIQ TSHSEVVSE MNGFQSLDG SSKIHVSKS GKKRRREKH KNRYSPDLG APSKEHKRK RKKKSLDFD DHMPHPRIT IKIKPIPLP AGEVASESN PQCFYVPNE NNDNAPPPI SLKVISLPE EMSPKDIEK NPVSDICDV CHTATVAST SVRCDDCQK TYHFTCLDP PLKKSPKKR GYSWHCADC DPTGSE
- >TC031364 RA
atgcccaac ctcctggag cgtgccgaa gacatcgat ttcgtcacg aaaacggcc attgagaga gacccgaat aagaggagg gttaagccg gttgagacc caagcctcg attctggac tttgatctc gaagaggag agctcggat gattcggat ttccggatt gaagaccac ccggaagtg agcgatgat gatgactcc gggaacagt gcttctgga ggtaatgac agcagtgga gaggaggaa tcagaagga tcagactta gacgaattg aaaaatttg aatttgccc aatggggac gggctatcc attgcggat gtcatccaa caagccaat ttgcagcta agttcgttc aatgaaaaa gacgcccaa attttgatc tgctgcggc tgtctcggg gaccaaagc gacggcgtc aacgaaatc gtcgaatgc gactcttgt ggggtcact gtccacgag gcctgttac ggcgtcgcg gactccgcc agtttgagc agcaccgac tctctgtcc ccgacagcc ccctggttt tgcgaggcg tgcaaagcc ggcgtgatg aaccctgtt tgcgagctg tgccccaat tcggggggc attttcaag gagacggac gtcgggaag tggattcat ctcgtttgc gctctctat gttcctgga gtggcattc ggcgaagtt gataaattg acaagtgtg actcttttc gaaatgcct tacagcaaa tggggggcg aaaagttgc agcttgtgt caggatgag cgctttgcc aggacgggg gtgtgcatc gggtgcgat gccggcatg tgcaagacc tacttccat gtcacatgt gcgcaacgc gagggtttt ttatcggaa gcgcactcc gaagaggtg gaccaagcg gaccccttt tacgcacat tgtaaatta cattcggac aagactttg gtgaaacgc agacgtaag aattatctg gcgttgcag ttgcgtacc cattacagg aaactgcaa tttgagaaa gaaggtcac agcgaaacc cctgaacaa gtgaggatt cagagaaaa cttgagaag cagaagttg cattttttg aaccacaaa gcgttaaaa ccgcctcca tgggtcccg acgcagaaa atgccaaga ttgctcacc accagcgcg tccgcttgt cgcaagtta ttacgcaag gccgagctg atggggatt aataccatg gtgttggag atgcaagaa gcccaagct gcggccctg gtggacgtc cgaaaaaag tggcacatt cctcccgcc tttaccgtc gaatttatc gcttattat ctcgatcgt aacgatcgg cttaaaggt atgaaacaa acccttgag aaattgctc gaggacaac cgcgatttg ctcgaagag cagcaactt ctgaggacg aagtacgat gagattttg aaaaaaagc gctgatgaa actaagaaa aacattgac ttgaaacaa acgatacat aagtatcat tcggcgatt ttgagcgct tgtcctacc aaaaatctg ccgaatatc gaggattta ggtaagcca caagtttcg agaactaat caacctatg atggtccca acagcagcc gccctgaaa atgggagtt ggttttccc ttgaataat attattcca gctgggaga accgatcgc gtgctcagc agtcacgcc aaacaagac tctctaatg ctcaacgaa tgcggcatc tgccgccaa aaacgtgac caacacttg ctcgcaaaa tgcgacaca tgccacctc tactaccac ttgaattgc ctaaatccg cccctgacc cgtcttccg aaaaaatcg aaactttac ggttggcaa tgttccgaa tgcgataaa acgtccgat tcggaagtc gaagaagtg aaaactcca cgacggagt aaaagtcgc tacagtcgt gaagcacgt tccgatccg gaagtttca actcctaaa ctcaaaatc aagccggta gaaccggtt tcggaacct acaccaaca cccacaccc acaccggaa atcgtccca attcagaca agtcatagt gaagtcgtg agcgaaatg aacgggttc caaagtttg gacggtagt agcaaaatt catgtatcc aagtcgggg aaaaagcga cggagggag aagcacaag aatcggtac tcccccgat ttgggggcc ccgagtaag gagcataag aggaagcgg aagaagaag agtctcgat tttgacgat catatgcca catccgagg attacgatc aaaatcaaa cctattcct ttgccggcc ggagaggtt gcgtctgag tcaaacccg cagtgtttt tacgtcccg aatgaaaac aacgataat gcgccgcct ccgatctcc ctcaaggtc atatcactg ccagaagaa atgtcacca aaagatata gagaaaaac ccggttagt gacatttgt gatgtttgt cacactgca actgtggct agtacttca gtcaggtgt gacgactgt caaaaaact taccatttt acgtgtctt gacccaccg ttgaagaaa agcccgaaa aaaagagga tattcgtgg cattgcgcc gattgtgac cctacgggg tccgagtaa
- >TC031364 RB
atgcccaac ctcctggag cgtgccgaa gacatcgat ttcgtcacg aaaacggcc agtaagtcg ccctccacc ccctcattt agtaaaatt cactataaa ctttcccgt gataaaaaa cgcttaaat ttatcaaca atcggggat ttgggcccc gagatgagt tcccttgag agagacccg aataagagg agggttaag ccggttgag acccaagcc tcgattctg gactttgat ctcgaagag gagagctcg gatgattcg gatttccgg attgaagac cacccggaa gtgagcgat gatgatgac tccgggaac agtgcttct ggaggtaat gacagcagt ggagaggag gaatcagaa ggatcagac ttagacgaa ttgaaaaat ttgaatttg cccaatggg gacgggcta tccattgcg gatgtcatc caacaagcc aatttgcag ctaagttcg ttcaatgaa aaagacgcc caaattttg atctgctgc ggctgtctc ggggaccaa agcgacggc gtcaacgaa atcgtcgaa tgcgactct tgtggggtc actgtccac gaggcctgt tacggcgtc gcggactcc gccagtttg agcagcacc gactctctg tccccgaca gccccctgg ttttgcgag gcgtgcaaa gccggcgtg atgaaccct gtttgcgag ctgtgcccc aattcgggg ggcattttc aaggagacg gacgtcggg aagtggatt catctcgtt tgcgctctc tatgttcct ggagtggca ttcggcgaa gttgataaa ttgacaagt gtgactctt ttcgaaatg ccttacagc aaatggggg gcgaaaagt tgcagcttg tgtcaggat gagcgcttt gccaggacg ggggtgtgc atcgggtgc gatgccggc atgtgcaag acctacttc catgtcaca tgtgcgcaa cgcgagggt tttttatcg gaagcgcac tccgaagag gtggaccaa gcggacccc ttttacgca cattgtaaa ttacattcg gacaagact ttggtgaaa cgcagacgt aagaattat ctggcgttg cagttgcgt acccattac aggaaactg caatttgag aaagaaggt cacagcgaa acccctgaa caagtgagg attcagaga aaacttgag aagcagaag ttgcatttt ttgaaccac aaagcgtta aaaccgcct ccatgggtc ccgacgcag aaaatgcca agattgctc accaccagc gcgtccgct tgtcgcaag ttattacgc aaggccgag ctgatgggg attaatacc atggtgttg gagatgcaa gaagcccaa gctgcggcc ctggtggac gtccgaaaa aagtggcac attcctccc gcctttacc gtcgaattt atcgcttat tatctcgat cgtaacgat cggcttaaa ggtatgaaa caaaccctt gagaaattg ctcgaggac aaccgcgat ttgctcgaa gagcagcaa cttctgagg acgaagtac gatgagatt ttgaaaaaa agcgctgat gaaactaag aaaaacatt gacttgaaa caaacgata cataagtat cattcggcg attttgagc gcttgtcct accaaaaat ctgccgaat atcgaggat ttaggtaag ccacaagtt tcgagaact aatcaacct atgatggtc ccaacagca gccgccctg aaaatggga gttggtttt cccttgaat aatattatt ccagctggg agaaccgat cgcgtgctc agcagtcac gccaaacaa gactctcta atgctcaac gaatgcggc atctgccgc caaaaacgt gaccaacac ttgctcgca aaatgcgac acatgccac ctctactac cacttgaat tgcctaaat ccgcccctg acccgtctt ccgaaaaaa tcgaaactt tacggttgg caatgttcc gaatgcgat aaaacgtcc gattcggaa gtcgaagaa gtgaaaact ccacgacgg agtaaaagt cgctacagt cgtgaagca cgttccgat ccggaagtt tcaactcct aaactcaaa atcaagccg gtagaaccg gtttcggaa cctacacca acacccaca cccacaccg gaaatcgtc ccaattcag acaagtcat agtgaagtc gtgagcgaa atgaacggg ttccaaagt ttggacggt agtagcaaa attcatgta tccaagtcg gggaaaaag cgacggagg gagaagcac aagaatcgg tactccccc gatttgggg gccccgagt aaggagcat aagaggaag cggaagaag aagagtctc gattttgac gatcatatg ccacatccg aggattacg atcaaaatc aaacctatt cctttgccg gccggagag gttgcgtct gagtcaaac ccgcagtgt ttttacgtc ccgaatgaa aacaacgat aatgcgccg cctccgatc tccctcaag gtcatatca ctgccagaa gaaatgtca ccaaaagat atagagaaa aacccggtt agtgacatt tgtgatgtt tgtcacact gcaactgtg gctagtact tcagtcagg tgtgacgac tgtcaaaaa acttaccat tttacgtgt cttgaccca ccgttgaag aaaagcccg aaaaaaaga ggatattcg tggcattgc gccgattgt gaccctacg gggtccgag taa
- >TC031364 RA
tctctttga caaaaacaa ttatcattt ttaatgaac ctaaaaaaa ttctttggg caaaaattc cccgaaatt ttcctttca agttcgaag gccgccctg gcagaaccc cattatcct tccatttcc ccccaaaat tcccccacc ggagtcgaa atcgccacc cgaatttaa gaagtacag cgcctcagc tcccacgag ggacctctt caccatcat gcccaacct cctggagcg tgccgaaga catcgattt cgtcacgaa aacggccat tgagagaga cccgaataa gaggagggt taagccggt tgagaccca agcctcgat tctggactt tgatctcga agaggagag ctcggatga ttcggattt ccggattga agaccaccc ggaagtgag cgatgatga tgactccgg gaacagtgc ttctggagg taatgacag cagtggaga ggaggaatc agaaggatc agacttaga cgaattgaa aaatttgaa tttgcccaa tggggacgg gctatccat tgcggatgt catccaaca agccaattt gcagctaag ttcgttcaa tgaaaaaga cgcccaaat tttgatctg ctgcggctg tctcgggga ccaaagcga cggcgtcaa cgaaatcgt cgaatgcga ctcttgtgg ggtcactgt ccacgaggc ctgttacgg cgtcgcgga ctccgccag tttgagcag caccgactc tctgtcccc gacagcccc ctggttttg cgaggcgtg caaagccgg cgtgatgaa ccctgtttg cgagctgtg ccccaattc ggggggcat tttcaagga gacggacgt cgggaagtg gattcatct cgtttgcgc tctctatgt tcctggagt ggcattcgg cgaagttga taaattgac aagtgtgac tcttttcga aatgcctta cagcaaatg gggggcgaa aagttgcag cttgtgtca ggatgagcg ctttgccag gacgggggt gtgcatcgg gtgcgatgc cggcatgtg caagaccta cttccatgt cacatgtgc gcaacgcga gggtttttt atcggaagc gcactccga agaggtgga ccaagcgga cccctttta cgcacattg taaattaca ttcggacaa gactttggt gaaacgcag acgtaagaa ttatctggc gttgcagtt gcgtaccca ttacaggaa actgcaatt tgagaaaga aggtcacag cgaaacccc tgaacaagt gaggattca gagaaaact tgagaagca gaagttgca ttttttgaa ccacaaagc gttaaaacc gcctccatg ggtcccgac gcagaaaat gccaagatt gctcaccac cagcgcgtc cgcttgtcg caagttatt acgcaaggc cgagctgat ggggattaa taccatggt gttggagat gcaagaagc ccaagctgc ggccctggt ggacgtccg aaaaaagtg gcacattcc tcccgcctt taccgtcga atttatcgc ttattatct cgatcgtaa cgatcggct taaaggtat gaaacaaac ccttgagaa attgctcga ggacaaccg cgatttgct cgaagagca gcaacttct gaggacgaa gtacgatga gattttgaa aaaaagcgc tgatgaaac taagaaaaa cattgactt gaaacaaac gatacataa gtatcattc ggcgatttt gagcgcttg tcctaccaa aaatctgcc gaatatcga ggatttagg taagccaca agtttcgag aactaatca acctatgat ggtcccaac agcagccgc cctgaaaat gggagttgg ttttccctt gaataatat tattccagc tgggagaac cgatcgcgt gctcagcag tcacgccaa acaagactc tctaatgct caacgaatg cggcatctg ccgccaaaa acgtgacca acacttgct cgcaaaatg cgacacatg ccacctcta ctaccactt gaattgcct aaatccgcc cctgacccg tcttccgaa aaaatcgaa actttacgg ttggcaatg ttccgaatg cgataaaac gtccgattc ggaagtcga agaagtgaa aactccacg acggagtaa aagtcgcta cagtcgtga agcacgttc cgatccgga agtttcaac tcctaaact caaaatcaa gccggtaga accggtttc ggaacctac accaacacc cacacccac accggaaat cgtcccaat tcagacaag tcatagtga agtcgtgag cgaaatgaa cgggttcca aagtttgga cggtagtag caaaattca tgtatccaa gtcggggaa aaagcgacg gagggagaa gcacaagaa tcggtactc ccccgattt gggggcccc gagtaagga gcataagag gaagcggaa gaagaagag tctcgattt tgacgatca tatgccaca tccgaggat tacgatcaa aatcaaacc tattccttt gccggccgg agaggttgc gtctgagtc aaacccgca gtgttttta cgtcccgaa tgaaaacaa cgataatgc gccgcctcc gatctccct caaggtcat atcactgcc agaagaaat gtcaccaaa agatataga gaaaaaccc ggttagtga catttgtga tgtttgtca cactgcaac tgtggctag tacttcagt caggtgtga cgactgtca aaaaactta ccattttac gtgtcttga cccaccgtt gaagaaaag cccgaaaaa aagaggata ttcgtggca ttgcgccga ttgtgaccc tacggggtc cgagtaagt gtttttcag ttattgcga attaattta gttatattt aattgcgtt tgtgaaata aaaactatt tttggcg
- >TC031364 RB
tctctttga caaaaacaa ttatcattt ttaatgaac ctaaaaaaa ttctttggg caaaaattc cccgaaatt ttcctttca agttcgaag gccgccctg gcagaaccc cattatcct tccatttcc ccccaaaat tcccccacc ggagtcgaa atcgccacc cgaatttaa gaagtacag cgcctcagc tcccacgag ggacctctt caccatcat gcccaacct cctggagcg tgccgaaga catcgattt cgtcacgaa aacggccag taagtcgcc ctccacccc ctcatttag taaaattca ctataaact ttcccgtga taaaaaacg cttaaattt atcaacaat cggggattt gggccccga gatgagttc ccttgagag agacccgaa taagaggag ggttaagcc ggttgagac ccaagcctc gattctgga ctttgatct cgaagagga gagctcgga tgattcgga tttccggat tgaagacca cccggaagt gagcgatga tgatgactc cgggaacag tgcttctgg aggtaatga cagcagtgg agaggagga atcagaagg atcagactt agacgaatt gaaaaattt gaatttgcc caatgggga cgggctatc cattgcgga tgtcatcca acaagccaa tttgcagct aagttcgtt caatgaaaa agacgccca aattttgat ctgctgcgg ctgtctcgg ggaccaaag cgacggcgt caacgaaat cgtcgaatg cgactcttg tggggtcac tgtccacga ggcctgtta cggcgtcgc ggactccgc cagtttgag cagcaccga ctctctgtc cccgacagc cccctggtt ttgcgaggc gtgcaaagc cggcgtgat gaaccctgt ttgcgagct gtgccccaa ttcgggggg cattttcaa ggagacgga cgtcgggaa gtggattca tctcgtttg cgctctcta tgttcctgg agtggcatt cggcgaagt tgataaatt gacaagtgt gactctttt cgaaatgcc ttacagcaa atggggggc gaaaagttg cagcttgtg tcaggatga gcgctttgc caggacggg ggtgtgcat cgggtgcga tgccggcat gtgcaagac ctacttcca tgtcacatg tgcgcaacg cgagggttt tttatcgga agcgcactc cgaagaggt ggaccaagc ggacccctt ttacgcaca ttgtaaatt acattcgga caagacttt ggtgaaacg cagacgtaa gaattatct ggcgttgca gttgcgtac ccattacag gaaactgca atttgagaa agaaggtca cagcgaaac ccctgaaca agtgaggat tcagagaaa acttgagaa gcagaagtt gcatttttt gaaccacaa agcgttaaa accgcctcc atgggtccc gacgcagaa aatgccaag attgctcac caccagcgc gtccgcttg tcgcaagtt attacgcaa ggccgagct gatggggat taataccat ggtgttgga gatgcaaga agcccaagc tgcggccct ggtggacgt ccgaaaaaa gtggcacat tcctcccgc ctttaccgt cgaatttat cgcttatta tctcgatcg taacgatcg gcttaaagg tatgaaaca aacccttga gaaattgct cgaggacaa ccgcgattt gctcgaaga gcagcaact tctgaggac gaagtacga tgagatttt gaaaaaaag cgctgatga aactaagaa aaacattga cttgaaaca aacgataca taagtatca ttcggcgat tttgagcgc ttgtcctac caaaaatct gccgaatat cgaggattt aggtaagcc acaagtttc gagaactaa tcaacctat gatggtccc aacagcagc cgccctgaa aatgggagt tggttttcc cttgaataa tattattcc agctgggag aaccgatcg cgtgctcag cagtcacgc caaacaaga ctctctaat gctcaacga atgcggcat ctgccgcca aaaacgtga ccaacactt gctcgcaaa atgcgacac atgccacct ctactacca cttgaattg cctaaatcc gcccctgac ccgtcttcc gaaaaaatc gaaacttta cggttggca atgttccga atgcgataa aacgtccga ttcggaagt cgaagaagt gaaaactcc acgacggag taaaagtcg ctacagtcg tgaagcacg ttccgatcc ggaagtttc aactcctaa actcaaaat caagccggt agaaccggt ttcggaacc tacaccaac acccacacc cacaccgga aatcgtccc aattcagac aagtcatag tgaagtcgt gagcgaaat gaacgggtt ccaaagttt ggacggtag tagcaaaat tcatgtatc caagtcggg gaaaaagcg acggaggga gaagcacaa gaatcggta ctcccccga tttgggggc cccgagtaa ggagcataa gaggaagcg gaagaagaa gagtctcga ttttgacga tcatatgcc acatccgag gattacgat caaaatcaa acctattcc tttgccggc cggagaggt tgcgtctga gtcaaaccc gcagtgttt ttacgtccc gaatgaaaa caacgataa tgcgccgcc tccgatctc cctcaaggt catatcact gccagaaga aatgtcacc aaaagatat agagaaaaa cccggttag tgacatttg tgatgtttg tcacactgc aactgtggc tagtacttc agtcaggtg tgacgactg tcaaaaaac ttaccattt tacgtgtct tgacccacc gttgaagaa aagcccgaa aaaaagagg atattcgtg gcattgcgc cgattgtga ccctacggg gtccgagta agtgttttt cagttattg cgaattaat ttagttata tttaattgc gtttgtgaa ataaaaact atttttggc g
