Status
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Gene information
General information
TC031364
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011301
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid21: 3079084..3087045
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031364
    iBeetle screen iB_01839
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031364 RA
      MPNLLERAE DIDFVTKTA IERDPNKRR VKPVETQAS 
      ILDFDLEEE SSDDSDFRI EDHPEVSDD DDSGNSASG 
      GNDSSGEEE SEGSDLDEL KNLNLPNGD GLSIADVIQ 
      QANLQLSSF NEKDAQILI CCGCLGDQS DGVNEIVEC 
      DSCGVTVHE ACYGVADSA SLSSTDSLS PTAPWFCEA 
      CKAGVMNPV CELCPNSGG IFKETDVGK WIHLVCALY 
      VPGVAFGEV DKLTSVTLF EMPYSKWGA KSCSLCQDE 
      RFARTGVCI GCDAGMCKT YFHVTCAQR EGFLSEAHS 
      EEVDQADPF YAHCKLHSD KTLVKRRRK NYLALQLRT 
      HYRKLQFEK EGHSETPEQ VRIQRKLEK QKLHFLNHK 
      ALKPPPWVP TQKMPRLLT TSASACRKL LRKAELMGI 
      NTMVLEMQE AQAAALVDV RKKWHIPPA FTVEFIAYY 
      LDRNDRLKG MKQTLEKLL EDNRDLLEE QQLLRTKYD 
      EILKKSADE TKKNIDLKQ TIHKYHSAI LSACPTKNL 
      PNIEDLGKP QVSRTNQPM MVPTAAALK MGVGFPLNN 
      IIPAGRTDR VLSSHAKQD SLMLNECGI CRQKRDQHL 
      LAKCDTCHL YYHLNCLNP PLTRLPKKS KLYGWQCSE 
      CDKTSDSEV EEVKTPRRS KSRYSREAR SDPEVSTPK 
      LKIKPVEPV SEPTPTPTP TPEIVPIQT SHSEVVSEM 
      NGFQSLDGS SKIHVSKSG KKRRREKHK NRYSPDLGA 
      PSKEHKRKR KKKSLDFDD HMPHPRITI KIKPIPLPA 
      GEVASESNP QCFYVPNEN NDNAPPPIS LKVISLPEE 
      MSPKDIEKN PVSDICDVC HTATVASTS VRCDDCQKT 
      YHFTCLDPP LKKSPKKRG YSWHCADCD PTGSE
    • >TC031364 RB
      MPNLLERAE DIDFVTKTA SKSPSTPSF SKIHYKLSR 
      DKKRLNLST IGDLGPEMS SLERDPNKR RVKPVETQA 
      SILDFDLEE ESSDDSDFR IEDHPEVSD DDDSGNSAS 
      GGNDSSGEE ESEGSDLDE LKNLNLPNG DGLSIADVI 
      QQANLQLSS FNEKDAQIL ICCGCLGDQ SDGVNEIVE 
      CDSCGVTVH EACYGVADS ASLSSTDSL SPTAPWFCE 
      ACKAGVMNP VCELCPNSG GIFKETDVG KWIHLVCAL 
      YVPGVAFGE VDKLTSVTL FEMPYSKWG AKSCSLCQD 
      ERFARTGVC IGCDAGMCK TYFHVTCAQ REGFLSEAH 
      SEEVDQADP FYAHCKLHS DKTLVKRRR KNYLALQLR 
      THYRKLQFE KEGHSETPE QVRIQRKLE KQKLHFLNH 
      KALKPPPWV PTQKMPRLL TTSASACRK LLRKAELMG 
      INTMVLEMQ EAQAAALVD VRKKWHIPP AFTVEFIAY 
      YLDRNDRLK GMKQTLEKL LEDNRDLLE EQQLLRTKY 
      DEILKKSAD ETKKNIDLK QTIHKYHSA ILSACPTKN 
      LPNIEDLGK PQVSRTNQP MMVPTAAAL KMGVGFPLN 
      NIIPAGRTD RVLSSHAKQ DSLMLNECG ICRQKRDQH 
      LLAKCDTCH LYYHLNCLN PPLTRLPKK SKLYGWQCS 
      ECDKTSDSE VEEVKTPRR SKSRYSREA RSDPEVSTP 
      KLKIKPVEP VSEPTPTPT PTPEIVPIQ TSHSEVVSE 
      MNGFQSLDG SSKIHVSKS GKKRRREKH KNRYSPDLG 
      APSKEHKRK RKKKSLDFD DHMPHPRIT IKIKPIPLP 
      AGEVASESN PQCFYVPNE NNDNAPPPI SLKVISLPE 
      EMSPKDIEK NPVSDICDV CHTATVAST SVRCDDCQK 
      TYHFTCLDP PLKKSPKKR GYSWHCADC DPTGSE
    CDS sequence
    • >TC031364 RA
      atgcccaac ctcctggag cgtgccgaa gacatcgat 
      ttcgtcacg aaaacggcc attgagaga gacccgaat 
      aagaggagg gttaagccg gttgagacc caagcctcg 
      attctggac tttgatctc gaagaggag agctcggat 
      gattcggat ttccggatt gaagaccac ccggaagtg 
      agcgatgat gatgactcc gggaacagt gcttctgga 
      ggtaatgac agcagtgga gaggaggaa tcagaagga 
      tcagactta gacgaattg aaaaatttg aatttgccc 
      aatggggac gggctatcc attgcggat gtcatccaa 
      caagccaat ttgcagcta agttcgttc aatgaaaaa 
      gacgcccaa attttgatc tgctgcggc tgtctcggg 
      gaccaaagc gacggcgtc aacgaaatc gtcgaatgc 
      gactcttgt ggggtcact gtccacgag gcctgttac 
      ggcgtcgcg gactccgcc agtttgagc agcaccgac 
      tctctgtcc ccgacagcc ccctggttt tgcgaggcg 
      tgcaaagcc ggcgtgatg aaccctgtt tgcgagctg 
      tgccccaat tcggggggc attttcaag gagacggac 
      gtcgggaag tggattcat ctcgtttgc gctctctat 
      gttcctgga gtggcattc ggcgaagtt gataaattg 
      acaagtgtg actcttttc gaaatgcct tacagcaaa 
      tggggggcg aaaagttgc agcttgtgt caggatgag 
      cgctttgcc aggacgggg gtgtgcatc gggtgcgat 
      gccggcatg tgcaagacc tacttccat gtcacatgt 
      gcgcaacgc gagggtttt ttatcggaa gcgcactcc 
      gaagaggtg gaccaagcg gaccccttt tacgcacat 
      tgtaaatta cattcggac aagactttg gtgaaacgc 
      agacgtaag aattatctg gcgttgcag ttgcgtacc 
      cattacagg aaactgcaa tttgagaaa gaaggtcac 
      agcgaaacc cctgaacaa gtgaggatt cagagaaaa 
      cttgagaag cagaagttg cattttttg aaccacaaa 
      gcgttaaaa ccgcctcca tgggtcccg acgcagaaa 
      atgccaaga ttgctcacc accagcgcg tccgcttgt 
      cgcaagtta ttacgcaag gccgagctg atggggatt 
      aataccatg gtgttggag atgcaagaa gcccaagct 
      gcggccctg gtggacgtc cgaaaaaag tggcacatt 
      cctcccgcc tttaccgtc gaatttatc gcttattat 
      ctcgatcgt aacgatcgg cttaaaggt atgaaacaa 
      acccttgag aaattgctc gaggacaac cgcgatttg 
      ctcgaagag cagcaactt ctgaggacg aagtacgat 
      gagattttg aaaaaaagc gctgatgaa actaagaaa 
      aacattgac ttgaaacaa acgatacat aagtatcat 
      tcggcgatt ttgagcgct tgtcctacc aaaaatctg 
      ccgaatatc gaggattta ggtaagcca caagtttcg 
      agaactaat caacctatg atggtccca acagcagcc 
      gccctgaaa atgggagtt ggttttccc ttgaataat 
      attattcca gctgggaga accgatcgc gtgctcagc 
      agtcacgcc aaacaagac tctctaatg ctcaacgaa 
      tgcggcatc tgccgccaa aaacgtgac caacacttg 
      ctcgcaaaa tgcgacaca tgccacctc tactaccac 
      ttgaattgc ctaaatccg cccctgacc cgtcttccg 
      aaaaaatcg aaactttac ggttggcaa tgttccgaa 
      tgcgataaa acgtccgat tcggaagtc gaagaagtg 
      aaaactcca cgacggagt aaaagtcgc tacagtcgt 
      gaagcacgt tccgatccg gaagtttca actcctaaa 
      ctcaaaatc aagccggta gaaccggtt tcggaacct 
      acaccaaca cccacaccc acaccggaa atcgtccca 
      attcagaca agtcatagt gaagtcgtg agcgaaatg 
      aacgggttc caaagtttg gacggtagt agcaaaatt 
      catgtatcc aagtcgggg aaaaagcga cggagggag 
      aagcacaag aatcggtac tcccccgat ttgggggcc 
      ccgagtaag gagcataag aggaagcgg aagaagaag 
      agtctcgat tttgacgat catatgcca catccgagg 
      attacgatc aaaatcaaa cctattcct ttgccggcc 
      ggagaggtt gcgtctgag tcaaacccg cagtgtttt 
      tacgtcccg aatgaaaac aacgataat gcgccgcct 
      ccgatctcc ctcaaggtc atatcactg ccagaagaa 
      atgtcacca aaagatata gagaaaaac ccggttagt 
      gacatttgt gatgtttgt cacactgca actgtggct 
      agtacttca gtcaggtgt gacgactgt caaaaaact 
      taccatttt acgtgtctt gacccaccg ttgaagaaa 
      agcccgaaa aaaagagga tattcgtgg cattgcgcc 
      gattgtgac cctacgggg tccgagtaa
    • >TC031364 RB
      atgcccaac ctcctggag cgtgccgaa gacatcgat 
      ttcgtcacg aaaacggcc agtaagtcg ccctccacc 
      ccctcattt agtaaaatt cactataaa ctttcccgt 
      gataaaaaa cgcttaaat ttatcaaca atcggggat 
      ttgggcccc gagatgagt tcccttgag agagacccg 
      aataagagg agggttaag ccggttgag acccaagcc 
      tcgattctg gactttgat ctcgaagag gagagctcg 
      gatgattcg gatttccgg attgaagac cacccggaa 
      gtgagcgat gatgatgac tccgggaac agtgcttct 
      ggaggtaat gacagcagt ggagaggag gaatcagaa 
      ggatcagac ttagacgaa ttgaaaaat ttgaatttg 
      cccaatggg gacgggcta tccattgcg gatgtcatc 
      caacaagcc aatttgcag ctaagttcg ttcaatgaa 
      aaagacgcc caaattttg atctgctgc ggctgtctc 
      ggggaccaa agcgacggc gtcaacgaa atcgtcgaa 
      tgcgactct tgtggggtc actgtccac gaggcctgt 
      tacggcgtc gcggactcc gccagtttg agcagcacc 
      gactctctg tccccgaca gccccctgg ttttgcgag 
      gcgtgcaaa gccggcgtg atgaaccct gtttgcgag 
      ctgtgcccc aattcgggg ggcattttc aaggagacg 
      gacgtcggg aagtggatt catctcgtt tgcgctctc 
      tatgttcct ggagtggca ttcggcgaa gttgataaa 
      ttgacaagt gtgactctt ttcgaaatg ccttacagc 
      aaatggggg gcgaaaagt tgcagcttg tgtcaggat 
      gagcgcttt gccaggacg ggggtgtgc atcgggtgc 
      gatgccggc atgtgcaag acctacttc catgtcaca 
      tgtgcgcaa cgcgagggt tttttatcg gaagcgcac 
      tccgaagag gtggaccaa gcggacccc ttttacgca 
      cattgtaaa ttacattcg gacaagact ttggtgaaa 
      cgcagacgt aagaattat ctggcgttg cagttgcgt 
      acccattac aggaaactg caatttgag aaagaaggt 
      cacagcgaa acccctgaa caagtgagg attcagaga 
      aaacttgag aagcagaag ttgcatttt ttgaaccac 
      aaagcgtta aaaccgcct ccatgggtc ccgacgcag 
      aaaatgcca agattgctc accaccagc gcgtccgct 
      tgtcgcaag ttattacgc aaggccgag ctgatgggg 
      attaatacc atggtgttg gagatgcaa gaagcccaa 
      gctgcggcc ctggtggac gtccgaaaa aagtggcac 
      attcctccc gcctttacc gtcgaattt atcgcttat 
      tatctcgat cgtaacgat cggcttaaa ggtatgaaa 
      caaaccctt gagaaattg ctcgaggac aaccgcgat 
      ttgctcgaa gagcagcaa cttctgagg acgaagtac 
      gatgagatt ttgaaaaaa agcgctgat gaaactaag 
      aaaaacatt gacttgaaa caaacgata cataagtat 
      cattcggcg attttgagc gcttgtcct accaaaaat 
      ctgccgaat atcgaggat ttaggtaag ccacaagtt 
      tcgagaact aatcaacct atgatggtc ccaacagca 
      gccgccctg aaaatggga gttggtttt cccttgaat 
      aatattatt ccagctggg agaaccgat cgcgtgctc 
      agcagtcac gccaaacaa gactctcta atgctcaac 
      gaatgcggc atctgccgc caaaaacgt gaccaacac 
      ttgctcgca aaatgcgac acatgccac ctctactac 
      cacttgaat tgcctaaat ccgcccctg acccgtctt 
      ccgaaaaaa tcgaaactt tacggttgg caatgttcc 
      gaatgcgat aaaacgtcc gattcggaa gtcgaagaa 
      gtgaaaact ccacgacgg agtaaaagt cgctacagt 
      cgtgaagca cgttccgat ccggaagtt tcaactcct 
      aaactcaaa atcaagccg gtagaaccg gtttcggaa 
      cctacacca acacccaca cccacaccg gaaatcgtc 
      ccaattcag acaagtcat agtgaagtc gtgagcgaa 
      atgaacggg ttccaaagt ttggacggt agtagcaaa 
      attcatgta tccaagtcg gggaaaaag cgacggagg 
      gagaagcac aagaatcgg tactccccc gatttgggg 
      gccccgagt aaggagcat aagaggaag cggaagaag 
      aagagtctc gattttgac gatcatatg ccacatccg 
      aggattacg atcaaaatc aaacctatt cctttgccg 
      gccggagag gttgcgtct gagtcaaac ccgcagtgt 
      ttttacgtc ccgaatgaa aacaacgat aatgcgccg 
      cctccgatc tccctcaag gtcatatca ctgccagaa 
      gaaatgtca ccaaaagat atagagaaa aacccggtt 
      agtgacatt tgtgatgtt tgtcacact gcaactgtg 
      gctagtact tcagtcagg tgtgacgac tgtcaaaaa 
      acttaccat tttacgtgt cttgaccca ccgttgaag 
      aaaagcccg aaaaaaaga ggatattcg tggcattgc 
      gccgattgt gaccctacg gggtccgag taa
    mRNA sequence
    • >TC031364 RA
      tctctttga caaaaacaa ttatcattt ttaatgaac 
      ctaaaaaaa ttctttggg caaaaattc cccgaaatt 
      ttcctttca agttcgaag gccgccctg gcagaaccc 
      cattatcct tccatttcc ccccaaaat tcccccacc 
      ggagtcgaa atcgccacc cgaatttaa gaagtacag 
      cgcctcagc tcccacgag ggacctctt caccatcat 
      gcccaacct cctggagcg tgccgaaga catcgattt 
      cgtcacgaa aacggccat tgagagaga cccgaataa 
      gaggagggt taagccggt tgagaccca agcctcgat 
      tctggactt tgatctcga agaggagag ctcggatga 
      ttcggattt ccggattga agaccaccc ggaagtgag 
      cgatgatga tgactccgg gaacagtgc ttctggagg 
      taatgacag cagtggaga ggaggaatc agaaggatc 
      agacttaga cgaattgaa aaatttgaa tttgcccaa 
      tggggacgg gctatccat tgcggatgt catccaaca 
      agccaattt gcagctaag ttcgttcaa tgaaaaaga 
      cgcccaaat tttgatctg ctgcggctg tctcgggga 
      ccaaagcga cggcgtcaa cgaaatcgt cgaatgcga 
      ctcttgtgg ggtcactgt ccacgaggc ctgttacgg 
      cgtcgcgga ctccgccag tttgagcag caccgactc 
      tctgtcccc gacagcccc ctggttttg cgaggcgtg 
      caaagccgg cgtgatgaa ccctgtttg cgagctgtg 
      ccccaattc ggggggcat tttcaagga gacggacgt 
      cgggaagtg gattcatct cgtttgcgc tctctatgt 
      tcctggagt ggcattcgg cgaagttga taaattgac 
      aagtgtgac tcttttcga aatgcctta cagcaaatg 
      gggggcgaa aagttgcag cttgtgtca ggatgagcg 
      ctttgccag gacgggggt gtgcatcgg gtgcgatgc 
      cggcatgtg caagaccta cttccatgt cacatgtgc 
      gcaacgcga gggtttttt atcggaagc gcactccga 
      agaggtgga ccaagcgga cccctttta cgcacattg 
      taaattaca ttcggacaa gactttggt gaaacgcag 
      acgtaagaa ttatctggc gttgcagtt gcgtaccca 
      ttacaggaa actgcaatt tgagaaaga aggtcacag 
      cgaaacccc tgaacaagt gaggattca gagaaaact 
      tgagaagca gaagttgca ttttttgaa ccacaaagc 
      gttaaaacc gcctccatg ggtcccgac gcagaaaat 
      gccaagatt gctcaccac cagcgcgtc cgcttgtcg 
      caagttatt acgcaaggc cgagctgat ggggattaa 
      taccatggt gttggagat gcaagaagc ccaagctgc 
      ggccctggt ggacgtccg aaaaaagtg gcacattcc 
      tcccgcctt taccgtcga atttatcgc ttattatct 
      cgatcgtaa cgatcggct taaaggtat gaaacaaac 
      ccttgagaa attgctcga ggacaaccg cgatttgct 
      cgaagagca gcaacttct gaggacgaa gtacgatga 
      gattttgaa aaaaagcgc tgatgaaac taagaaaaa 
      cattgactt gaaacaaac gatacataa gtatcattc 
      ggcgatttt gagcgcttg tcctaccaa aaatctgcc 
      gaatatcga ggatttagg taagccaca agtttcgag 
      aactaatca acctatgat ggtcccaac agcagccgc 
      cctgaaaat gggagttgg ttttccctt gaataatat 
      tattccagc tgggagaac cgatcgcgt gctcagcag 
      tcacgccaa acaagactc tctaatgct caacgaatg 
      cggcatctg ccgccaaaa acgtgacca acacttgct 
      cgcaaaatg cgacacatg ccacctcta ctaccactt 
      gaattgcct aaatccgcc cctgacccg tcttccgaa 
      aaaatcgaa actttacgg ttggcaatg ttccgaatg 
      cgataaaac gtccgattc ggaagtcga agaagtgaa 
      aactccacg acggagtaa aagtcgcta cagtcgtga 
      agcacgttc cgatccgga agtttcaac tcctaaact 
      caaaatcaa gccggtaga accggtttc ggaacctac 
      accaacacc cacacccac accggaaat cgtcccaat 
      tcagacaag tcatagtga agtcgtgag cgaaatgaa 
      cgggttcca aagtttgga cggtagtag caaaattca 
      tgtatccaa gtcggggaa aaagcgacg gagggagaa 
      gcacaagaa tcggtactc ccccgattt gggggcccc 
      gagtaagga gcataagag gaagcggaa gaagaagag 
      tctcgattt tgacgatca tatgccaca tccgaggat 
      tacgatcaa aatcaaacc tattccttt gccggccgg 
      agaggttgc gtctgagtc aaacccgca gtgttttta 
      cgtcccgaa tgaaaacaa cgataatgc gccgcctcc 
      gatctccct caaggtcat atcactgcc agaagaaat 
      gtcaccaaa agatataga gaaaaaccc ggttagtga 
      catttgtga tgtttgtca cactgcaac tgtggctag 
      tacttcagt caggtgtga cgactgtca aaaaactta 
      ccattttac gtgtcttga cccaccgtt gaagaaaag 
      cccgaaaaa aagaggata ttcgtggca ttgcgccga 
      ttgtgaccc tacggggtc cgagtaagt gtttttcag 
      ttattgcga attaattta gttatattt aattgcgtt 
      tgtgaaata aaaactatt tttggcg
    • >TC031364 RB
      tctctttga caaaaacaa ttatcattt ttaatgaac 
      ctaaaaaaa ttctttggg caaaaattc cccgaaatt 
      ttcctttca agttcgaag gccgccctg gcagaaccc 
      cattatcct tccatttcc ccccaaaat tcccccacc 
      ggagtcgaa atcgccacc cgaatttaa gaagtacag 
      cgcctcagc tcccacgag ggacctctt caccatcat 
      gcccaacct cctggagcg tgccgaaga catcgattt 
      cgtcacgaa aacggccag taagtcgcc ctccacccc 
      ctcatttag taaaattca ctataaact ttcccgtga 
      taaaaaacg cttaaattt atcaacaat cggggattt 
      gggccccga gatgagttc ccttgagag agacccgaa 
      taagaggag ggttaagcc ggttgagac ccaagcctc 
      gattctgga ctttgatct cgaagagga gagctcgga 
      tgattcgga tttccggat tgaagacca cccggaagt 
      gagcgatga tgatgactc cgggaacag tgcttctgg 
      aggtaatga cagcagtgg agaggagga atcagaagg 
      atcagactt agacgaatt gaaaaattt gaatttgcc 
      caatgggga cgggctatc cattgcgga tgtcatcca 
      acaagccaa tttgcagct aagttcgtt caatgaaaa 
      agacgccca aattttgat ctgctgcgg ctgtctcgg 
      ggaccaaag cgacggcgt caacgaaat cgtcgaatg 
      cgactcttg tggggtcac tgtccacga ggcctgtta 
      cggcgtcgc ggactccgc cagtttgag cagcaccga 
      ctctctgtc cccgacagc cccctggtt ttgcgaggc 
      gtgcaaagc cggcgtgat gaaccctgt ttgcgagct 
      gtgccccaa ttcgggggg cattttcaa ggagacgga 
      cgtcgggaa gtggattca tctcgtttg cgctctcta 
      tgttcctgg agtggcatt cggcgaagt tgataaatt 
      gacaagtgt gactctttt cgaaatgcc ttacagcaa 
      atggggggc gaaaagttg cagcttgtg tcaggatga 
      gcgctttgc caggacggg ggtgtgcat cgggtgcga 
      tgccggcat gtgcaagac ctacttcca tgtcacatg 
      tgcgcaacg cgagggttt tttatcgga agcgcactc 
      cgaagaggt ggaccaagc ggacccctt ttacgcaca 
      ttgtaaatt acattcgga caagacttt ggtgaaacg 
      cagacgtaa gaattatct ggcgttgca gttgcgtac 
      ccattacag gaaactgca atttgagaa agaaggtca 
      cagcgaaac ccctgaaca agtgaggat tcagagaaa 
      acttgagaa gcagaagtt gcatttttt gaaccacaa 
      agcgttaaa accgcctcc atgggtccc gacgcagaa 
      aatgccaag attgctcac caccagcgc gtccgcttg 
      tcgcaagtt attacgcaa ggccgagct gatggggat 
      taataccat ggtgttgga gatgcaaga agcccaagc 
      tgcggccct ggtggacgt ccgaaaaaa gtggcacat 
      tcctcccgc ctttaccgt cgaatttat cgcttatta 
      tctcgatcg taacgatcg gcttaaagg tatgaaaca 
      aacccttga gaaattgct cgaggacaa ccgcgattt 
      gctcgaaga gcagcaact tctgaggac gaagtacga 
      tgagatttt gaaaaaaag cgctgatga aactaagaa 
      aaacattga cttgaaaca aacgataca taagtatca 
      ttcggcgat tttgagcgc ttgtcctac caaaaatct 
      gccgaatat cgaggattt aggtaagcc acaagtttc 
      gagaactaa tcaacctat gatggtccc aacagcagc 
      cgccctgaa aatgggagt tggttttcc cttgaataa 
      tattattcc agctgggag aaccgatcg cgtgctcag 
      cagtcacgc caaacaaga ctctctaat gctcaacga 
      atgcggcat ctgccgcca aaaacgtga ccaacactt 
      gctcgcaaa atgcgacac atgccacct ctactacca 
      cttgaattg cctaaatcc gcccctgac ccgtcttcc 
      gaaaaaatc gaaacttta cggttggca atgttccga 
      atgcgataa aacgtccga ttcggaagt cgaagaagt 
      gaaaactcc acgacggag taaaagtcg ctacagtcg 
      tgaagcacg ttccgatcc ggaagtttc aactcctaa 
      actcaaaat caagccggt agaaccggt ttcggaacc 
      tacaccaac acccacacc cacaccgga aatcgtccc 
      aattcagac aagtcatag tgaagtcgt gagcgaaat 
      gaacgggtt ccaaagttt ggacggtag tagcaaaat 
      tcatgtatc caagtcggg gaaaaagcg acggaggga 
      gaagcacaa gaatcggta ctcccccga tttgggggc 
      cccgagtaa ggagcataa gaggaagcg gaagaagaa 
      gagtctcga ttttgacga tcatatgcc acatccgag 
      gattacgat caaaatcaa acctattcc tttgccggc 
      cggagaggt tgcgtctga gtcaaaccc gcagtgttt 
      ttacgtccc gaatgaaaa caacgataa tgcgccgcc 
      tccgatctc cctcaaggt catatcact gccagaaga 
      aatgtcacc aaaagatat agagaaaaa cccggttag 
      tgacatttg tgatgtttg tcacactgc aactgtggc 
      tagtacttc agtcaggtg tgacgactg tcaaaaaac 
      ttaccattt tacgtgtct tgacccacc gttgaagaa 
      aagcccgaa aaaaagagg atattcgtg gcattgcgc 
      cgattgtga ccctacggg gtccgagta agtgttttt 
      cagttattg cgaattaat ttagttata tttaattgc 
      gtttgtgaa ataaaaact atttttggc g