Status
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Gene information
General information
TC031362
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011299,TC011300
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19322166..19459665
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031362
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01838

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031362 RA
      MGAQKCFSV GLVLAFSAF CVHSQSIDE KIKLDTDLS 
      QFYNQLERS KLANVTLQY KQATIFAPI NEAFQRMEA 
      TEANPDDLV LYHMTSIPR KTTQLGTSY TSMNSELEG 
      NPPLWITHT TGNYHNDIY VNNARLLVS HSDIMTTQK 
      NGNIQVVHK IDEVLVPTR SAKNAQHRV YNPTAYEFL 
      EYYESLIFH PHRLRYFRQ KVQQHSKQD IFKTEGGHT 
      FFIPVDEGF KNKRAELID GKIIDGHVI PKHVLFTRP 
      TKKDVPFQT LANGDNNVR VVISFTQET RGPNNQVIN 
      YVKSHTLLS DGKHSQGVV LSEIVKANI PVKNGVVHL 
      IQKPLMVVD NNVRELLEE KDGGILSSF VKAINDADY 
      REFYNTLER SQDITLFAP CNNAWTDDF TLNSIISNK 
      NRFKDILMM HLVPDNRLY VDRILSISH GRDKRVHTR 
      DPRNDLYFN VVTSGTNRT VTVEGGGVN ATVIQPDLA 
      ATNGIIHVI DRVLGVPYT TVYDKLRTD YSLNYTFRL 
      GNQGGFNNQ LSDTTKKFT YFVPRNQAW DEASVDHPS 
      AIKKLFMPE FHYHATNIL ERHLVISDE PYTMERIKQ 
      LTLKSNQNM NNYERRTEI QLPTIRGEL NLYVEELQD 
      HTFIIHWKG ERIPVFKPN VACTNGVIH VIDAPFLQD 
      GDIRVSGAP SLHFAPHLI MILAAKWLL L
    • >TC031362 RB
      MGAQKCFSV GLVLAFSAF CVHSQSIDE KIKLDTDLS 
      QFKYENSFS YNQVRMWYV LGAIMGGQK CFFLGLLIT 
      FSGFGVHLC FSPSLIEKL TLDVEFAQF YNQLERSKL 
      ANVTLQYKQ ATIFAPINE AFQRMEATE ANPDDLVLY 
      HMTSIPRKT TQLGTSYTS MNSELEGNP PLWITHTTG 
      NYHNDIYVN NARLLVSHS DIMTTQKNG NIQVVHKID 
      EVLVPTRSA KNAQHRVYN PTAYEFLEY YESLIFHPH 
      RLRYFRQKV QQHSKQDIF KTEGGHTFF IPVDEGFKN 
      KRAELIDGK IIDGHVIPK HVLFTRPTK KDVPFQTLA 
      NGDNNVRVV ISFTQETRG PNNQVINYV KSHTLLSDG 
      KHSQGVVLS EIVKANIPV KNGVVHLIQ KPLMVVDNN 
      VRELLEEKD GGILSSFVK AINDADYRE FYNTLERSQ 
      DITLFAPCN NAWTDDFTL NSIISNKNR FKDILMMHL 
      VPDNRLYVD RILSISHGR DKRVHTRDP RNDLYFNVV 
      TSGTNRTVT VEGGGVNAT VIQPDLAAT NGIIHVIDR 
      VLGVPYTTV YDKLRTDYS LNYTFRLGN QGGFNNQLS 
      DTTKKFTYF VPRNQAWDE ASVDHPSAI KKLFMPEFH 
      YHATNILER HLVISDEPY TMERIKQLT LKSNQNMNN 
      YERRTEIQL PTIRGELNL YVEELQDHT FIIHWKGER 
      IPVFKPNVA CTNGVIHVI DAPFLQDGD IRVSGAPSL 
      HFAPHLIMI LAAKWLLL
    CDS sequence
    • >TC031362 RA
      atgggggcg caaaagtgc ttttccgtg ggacttgtt 
      ctagctttc tcggctttt tgcgtccac tcgcagtcc 
      atcgacgag aaaatcaag ctagatacg gatttgtct 
      cagttttac aaccaattg gagcgcagt aagctggcc 
      aatgtgacc ctccaatac aagcaagcg acaatcttt 
      gcccccata aacgaggcc ttccaaaga atggaagct 
      acggaggca aacccggac gacctagtt ctctaccac 
      atgacttca atcccgcgt aagaccacc caactgggc 
      acctcctac acgtccatg aactccgag ctggaggga 
      aatccccca ctttggatc acacacaca acggggaat 
      taccacaac gatatctac gtgaacaat gcccgactc 
      cttgtcagc cattcggat atcatgacg acgcagaag 
      aatggcaac attcaggtt gtacataaa attgacgag 
      gtcttggtt cccacgaga tcagccaag aacgcacaa 
      catcgggtt tacaaccct acagcgtac gaatttcta 
      gagtactac gagtccctc atctttcat ccccatcga 
      ttgaggtat ttccggcaa aaagtacaa cagcacagc 
      aaacaggat atttttaaa acggaggga gggcacacg 
      tttttcatc cccgtcgac gagggattt aagaacaag 
      cgcgccgaa ctaatcgac ggcaaaatc atcgacggc 
      cacgtcatc cccaagcac gtcctgttc accaggcct 
      acaaaaaaa gacgttccc ttccaaacc ctcgccaac 
      ggagacaac aacgttcga gttgttatt tcgttcacg 
      caagaaacg cgaggcccc aataaccaa gtcataaat 
      tacgtgaag agtcatact ctgttgagc gacggcaag 
      cactcccag ggggttgtt ttgtctgaa atagtcaaa 
      gccaacatc cccgtcaaa aacggggtg gttcacctc 
      atccagaag cccttgatg gtggtggat aacaacgtg 
      cgggagctt ttggaggag aaagacggg ggcattctt 
      agcagcttc gtgaaagcc atcaacgac gccgattac 
      cgggagttt tacaacacg ctggagcgg tctcaggat 
      atcaccctg ttcgcgcct tgcaacaac gcctggacg 
      gacgacttc accctcaac agcatcatc agcaacaag 
      aaccgattt aaggacatc ctgatgatg cacctggtg 
      cccgataat cggctgtat gtcgacagg atcctcagt 
      attagccac ggcagagac aagagggtg cacactcgc 
      gacccccgc aacgacctc tacttcaat gtagtaaca 
      tccggcacc aaccgcacc gtcaccgtc gaaggaggc 
      ggcgttaac gccactgta attcaacca gacctcgca 
      gccacaaat ggtatcatt cacgtaatc gatagagtg 
      ttgggagtt ccttatacc acagtctac gacaagtta 
      cgaacggat tattcactc aactatacg ttccgtctg 
      ggtaatcaa ggcggcttc aacaaccaa ctcagcgat 
      accaccaaa aagtttact tacttcgtt ccccgaaac 
      caggcctgg gacgaagcc agcgtggac cacccctca 
      gcaatcaaa aaactcttc atgccggaa tttcactac 
      cacgccact aacatcctc gagcgccac ctggtgatc 
      tccgacgag ccctacacc atggaacgc atcaaacaa 
      ctaacactg aaatccaac caaaacatg aacaactac 
      gagcgaaga accgaaatc caactcccg accatacgc 
      ggcgaactt aatctatac gtggaggag ttgcaagat 
      cacactttc ataatccac tggaaaggc gaaagaatc 
      cccgtattc aagccaaat gtagcatgc accaacgga 
      gtcatccac gtcatcgac gcccctttt ctgcaagac 
      ggcgacatc cgagtcagc ggcgcccca tctctgcat 
      tttgcgccc catttaata atgattttg gccgccaaa 
      tggttatta ctttaa
    • >TC031362 RB
      atgggggcg caaaagtgc ttttccgtg ggacttgtt 
      ctagctttc tcggctttt tgcgtccac tcgcagtcc 
      atcgacgag aaaatcaag ctagatacg gatttgtct 
      cagtttaaa tatgagaat tcgtttagt tacaaccaa 
      gtacgaatg tggtacgtt cttggtgca ataatgggt 
      gggcaaaag tgctttttc ctcggactt ttaataact 
      ttttcgggt tttggcgtc catttgtgc ttttcgccg 
      tctctcatc gagaaactt acattggat gttgagttt 
      gctcagttt tacaaccaa ttggagcgc agtaagctg 
      gccaatgtg accctccaa tacaagcaa gcgacaatc 
      tttgccccc ataaacgag gccttccaa agaatggaa 
      gctacggag gcaaacccg gacgaccta gttctctac 
      cacatgact tcaatcccg cgtaagacc acccaactg 
      ggcacctcc tacacgtcc atgaactcc gagctggag 
      ggaaatccc ccactttgg atcacacac acaacgggg 
      aattaccac aacgatatc tacgtgaac aatgcccga 
      ctccttgtc agccattcg gatatcatg acgacgcag 
      aagaatggc aacattcag gttgtacat aaaattgac 
      gaggtcttg gttcccacg agatcagcc aagaacgca 
      caacatcgg gtttacaac cctacagcg tacgaattt 
      ctagagtac tacgagtcc ctcatcttt catccccat 
      cgattgagg tatttccgg caaaaagta caacagcac 
      agcaaacag gatattttt aaaacggag ggagggcac 
      acgtttttc atccccgtc gacgaggga tttaagaac 
      aagcgcgcc gaactaatc gacggcaaa atcatcgac 
      ggccacgtc atccccaag cacgtcctg ttcaccagg 
      cctacaaaa aaagacgtt cccttccaa accctcgcc 
      aacggagac aacaacgtt cgagttgtt atttcgttc 
      acgcaagaa acgcgaggc cccaataac caagtcata 
      aattacgtg aagagtcat actctgttg agcgacggc 
      aagcactcc cagggggtt gttttgtct gaaatagtc 
      aaagccaac atccccgtc aaaaacggg gtggttcac 
      ctcatccag aagcccttg atggtggtg gataacaac 
      gtgcgggag cttttggag gagaaagac gggggcatt 
      cttagcagc ttcgtgaaa gccatcaac gacgccgat 
      taccgggag ttttacaac acgctggag cggtctcag 
      gatatcacc ctgttcgcg ccttgcaac aacgcctgg 
      acggacgac ttcaccctc aacagcatc atcagcaac 
      aagaaccga tttaaggac atcctgatg atgcacctg 
      gtgcccgat aatcggctg tatgtcgac aggatcctc 
      agtattagc cacggcaga gacaagagg gtgcacact 
      cgcgacccc cgcaacgac ctctacttc aatgtagta 
      acatccggc accaaccgc accgtcacc gtcgaagga 
      ggcggcgtt aacgccact gtaattcaa ccagacctc 
      gcagccaca aatggtatc attcacgta atcgataga 
      gtgttggga gttccttat accacagtc tacgacaag 
      ttacgaacg gattattca ctcaactat acgttccgt 
      ctgggtaat caaggcggc ttcaacaac caactcagc 
      gataccacc aaaaagttt acttacttc gttccccga 
      aaccaggcc tgggacgaa gccagcgtg gaccacccc 
      tcagcaatc aaaaaactc ttcatgccg gaatttcac 
      taccacgcc actaacatc ctcgagcgc cacctggtg 
      atctccgac gagccctac accatggaa cgcatcaaa 
      caactaaca ctgaaatcc aaccaaaac atgaacaac 
      tacgagcga agaaccgaa atccaactc ccgaccata 
      cgcggcgaa cttaatcta tacgtggag gagttgcaa 
      gatcacact ttcataatc cactggaaa ggcgaaaga 
      atccccgta ttcaagcca aatgtagca tgcaccaac 
      ggagtcatc cacgtcatc gacgcccct tttctgcaa 
      gacggcgac atccgagtc agcggcgcc ccatctctg 
      cattttgcg ccccattta ataatgatt ttggccgcc 
      aaatggtta ttactttaa
    mRNA sequence
    • >TC031362 RA
      attcagggc agaattgaa tgttttggg ttttttcga 
      aggagagtg ttatttttc aggtcgtaa acgccgcat 
      gggtctgct tccccgcaa ctatatggt gggttatct 
      gacctaatc ttacgtctt tcgcttctg aagccacat 
      gcagtgtca gtccagccg tgcttgagt cagtcttcg 
      ctaggaaaa aattaagct aagtttaat tatgtcaga 
      ctatatatt ttctttatg tgtcgttat tttatagtt 
      ttaacgaga tattttatc gagttctcc tctgtcaag 
      gtttgttgt ttatctaga gatatagca aagtattgg 
      atactaaaa accaaacag attgattat ttagtttat 
      cactgccaa aagttcgac aattgcttt tagtaatgt 
      aaaaaccct acttgtgat aaacttatt ggaggcgaa 
      ctaaatgta ttttaagac agttgtaac ctattttaa 
      tataatttt tagtattta cgatttttc tgtaaatat 
      tgtttgcat tgtggtagc ttaatgcat catctaaga 
      ctaaaaatc atcacatgg agcaaggtt gtgacttac 
      ggaccaaac gaatcaaaa ttgtgctcc cgctttcct 
      caactcatt cacattttc aggacttcg cacttgcat 
      taaatgtag ccgtagtat tgtaattta cataaaata 
      aaatgttga acaactgga atcgaccac ttttttcat 
      cctcctcac gacactttg acccgccca cgtcgctgt 
      cggtttctt gtagggtgt gtgtgattc cgtcttctc 
      ttgagtgtc aacgaacta cgagtgtgg tgcgttttt 
      ggtgcaata atgggggcg caaaagtgc ttttccgtg 
      ggacttgtt ctagctttc tcggctttt tgcgtccac 
      tcgcagtcc atcgacgag aaaatcaag ctagatacg 
      gatttgtct cagttttac aaccaattg gagcgcagt 
      aagctggcc aatgtgacc ctccaatac aagcaagcg 
      acaatcttt gcccccata aacgaggcc ttccaaaga 
      atggaagct acggaggca aacccggac gacctagtt 
      ctctaccac atgacttca atcccgcgt aagaccacc 
      caactgggc acctcctac acgtccatg aactccgag 
      ctggaggga aatccccca ctttggatc acacacaca 
      acggggaat taccacaac gatatctac gtgaacaat 
      gcccgactc cttgtcagc cattcggat atcatgacg 
      acgcagaag aatggcaac attcaggtt gtacataaa 
      attgacgag gtcttggtt cccacgaga tcagccaag 
      aacgcacaa catcgggtt tacaaccct acagcgtac 
      gaatttcta gagtactac gagtccctc atctttcat 
      ccccatcga ttgaggtat ttccggcaa aaagtacaa 
      cagcacagc aaacaggat atttttaaa acggaggga 
      gggcacacg tttttcatc cccgtcgac gagggattt 
      aagaacaag cgcgccgaa ctaatcgac ggcaaaatc 
      atcgacggc cacgtcatc cccaagcac gtcctgttc 
      accaggcct acaaaaaaa gacgttccc ttccaaacc 
      ctcgccaac ggagacaac aacgttcga gttgttatt 
      tcgttcacg caagaaacg cgaggcccc aataaccaa 
      gtcataaat tacgtgaag agtcatact ctgttgagc 
      gacggcaag cactcccag ggggttgtt ttgtctgaa 
      atagtcaaa gccaacatc cccgtcaaa aacggggtg 
      gttcacctc atccagaag cccttgatg gtggtggat 
      aacaacgtg cgggagctt ttggaggag aaagacggg 
      ggcattctt agcagcttc gtgaaagcc atcaacgac 
      gccgattac cgggagttt tacaacacg ctggagcgg 
      tctcaggat atcaccctg ttcgcgcct tgcaacaac 
      gcctggacg gacgacttc accctcaac agcatcatc 
      agcaacaag aaccgattt aaggacatc ctgatgatg 
      cacctggtg cccgataat cggctgtat gtcgacagg 
      atcctcagt attagccac ggcagagac aagagggtg 
      cacactcgc gacccccgc aacgacctc tacttcaat 
      gtagtaaca tccggcacc aaccgcacc gtcaccgtc 
      gaaggaggc ggcgttaac gccactgta attcaacca 
      gacctcgca gccacaaat ggtatcatt cacgtaatc 
      gatagagtg ttgggagtt ccttatacc acagtctac 
      gacaagtta cgaacggat tattcactc aactatacg 
      ttccgtctg ggtaatcaa ggcggcttc aacaaccaa 
      ctcagcgat accaccaaa aagtttact tacttcgtt 
      ccccgaaac caggcctgg gacgaagcc agcgtggac 
      cacccctca gcaatcaaa aaactcttc atgccggaa 
      tttcactac cacgccact aacatcctc gagcgccac 
      ctggtgatc tccgacgag ccctacacc atggaacgc 
      atcaaacaa ctaacactg aaatccaac caaaacatg 
      aacaactac gagcgaaga accgaaatc caactcccg 
      accatacgc ggcgaactt aatctatac gtggaggag 
      ttgcaagat cacactttc ataatccac tggaaaggc 
      gaaagaatc cccgtattc aagccaaat gtagcatgc 
      accaacgga gtcatccac gtcatcgac gcccctttt 
      ctgcaagac ggcgacatc cgagtcagc ggcgcccca 
      tctctgcat tttgcgccc catttaata atgattttg 
      gccgccaaa tggttatta ctttaatgg cgtccgctt 
      ttttagtca tagactcgt gagaactta atacaatgt 
      aacggtcgg tctgtgaag attttccaa aaagtttgt 
      ttgattatt tggacccag tttgcgttc tttccattc 
      tttcaggcg ggattcttc acagacgcc ctgatatat 
      caataaacc acgtttttt gaataatcc ataatcaat 
      tacttgcac caatgcgac ataatttct atcatacct 
      tttagattg tattattta cgatttgta aataagtca 
      attttttaa ttttgcatt tgtatatag gtaggaccc 
      aaagttaga ctactttaa ctcggaaat tctaaacca 
      attggccgc gatcccgat gaagcattt cagattatt 
      tcccccaaa aaactctaa tcaatgttg acgaaatca 
      aaatattta ttaaacaaa ctatgcctt ctaatctgt 
      tgaataatt ttctaaaaa ttgttgact gaattgaat 
      tttctctat atcacgttt ctaaatgta aaaatgaaa 
      tcatttcgt agtgccctt aaacttata cattccact 
      ccaacaaac tgttgcttc actttggtt gaacccagc 
      tgtaggggg cgctcaagt cggcagtcg gtacctgta 
      atccagcag cgcatatcc tgtgtttgc gcccccttc 
      cgggcgtat gattggacg aattgctta tttaaaatg 
      ttgtaaata cgtgtggat gtgtataaa tgttcatat 
      ttttgacag taaatgttt ataaatgtt agataagga 
      aaagttata ggtaggttt tgctttccg tagtctatt 
      ataggtctt tgggcactg aaatgcttc acatttaat 
      gaaaatctg acctcgagg gtcgaagca caagttttt 
      aaaaccctc ggtgtgaga ttttgttag cgaacgaaa 
      ccaaagaaa tagcccatt ctgactgaa atgttttta 
      aaaactaat taatcaatg tcaaatata agtgtataa 
      gaactgacc gtaccatat aatagatgt ttaaagctt 
      aactatgtt agggaactt tcgaacgtt tagactata 
      aatgtgtgt gtaagaaaa taattgcac tgccaatgt 
      gaacctaaa ttttacgta gtcgagctg ttttctttc 
      acttgaccc tcttcgttt caacttgtt ttagctagg 
      attgtatat aatattttc tttccaata aagcgttca 
      gtgctct
    • >TC031362 RB
      attcagggc agaattgaa tgttttggg ttttttcga 
      aggagagtg ttatttttc aggtcgtaa acgccgcat 
      gggtctgct tccccgcaa ctatatggt gggttatct 
      gacctaatc ttacgtctt tcgcttctg aagccacat 
      gcagtgtca gtccagccg tgcttgagt cagtcttcg 
      ctaggaaaa aattaagct aagtttaat tatgtcaga 
      ctatatatt ttctttatg tgtcgttat tttatagtt 
      ttaacgaga tattttatc gagttctcc tctgtcaag 
      gtttgttgt ttatctaga gatatagca aagtattgg 
      atactaaaa accaaacag attgattat ttagtttat 
      cactgccaa aagttcgac aattgcttt tagtaatgt 
      aaaaaccct acttgtgat aaacttatt ggaggcgaa 
      ctaaatgta ttttaagac agttgtaac ctattttaa 
      tataatttt tagtattta cgatttttc tgtaaatat 
      tgtttgcat tgtggtagc ttaatgcat catctaaga 
      ctaaaaatc atcacatgg agcaaggtt gtgacttac 
      ggaccaaac gaatcaaaa ttgtgctcc cgctttcct 
      caactcatt cacattttc aggacttcg cacttgcat 
      taaatgtag ccgtagtat tgtaattta cataaaata 
      aaatgttga acaactgga atcgaccac ttttttcat 
      cctcctcac gacactttg acccgccca cgtcgctgt 
      cggtttctt gtagggtgt gtgtgattc cgtcttctc 
      ttgagtgtc aacgaacta cgagtgtgg tgcgttttt 
      ggtgcaata atgggggcg caaaagtgc ttttccgtg 
      ggacttgtt ctagctttc tcggctttt tgcgtccac 
      tcgcagtcc atcgacgag aaaatcaag ctagatacg 
      gatttgtct cagtttaaa tatgagaat tcgtttagt 
      tacaaccaa gtacgaatg tggtacgtt cttggtgca 
      ataatgggt gggcaaaag tgctttttc ctcggactt 
      ttaataact ttttcgggt tttggcgtc catttgtgc 
      ttttcgccg tctctcatc gagaaactt acattggat 
      gttgagttt gctcagttt tacaaccaa ttggagcgc 
      agtaagctg gccaatgtg accctccaa tacaagcaa 
      gcgacaatc tttgccccc ataaacgag gccttccaa 
      agaatggaa gctacggag gcaaacccg gacgaccta 
      gttctctac cacatgact tcaatcccg cgtaagacc 
      acccaactg ggcacctcc tacacgtcc atgaactcc 
      gagctggag ggaaatccc ccactttgg atcacacac 
      acaacgggg aattaccac aacgatatc tacgtgaac 
      aatgcccga ctccttgtc agccattcg gatatcatg 
      acgacgcag aagaatggc aacattcag gttgtacat 
      aaaattgac gaggtcttg gttcccacg agatcagcc 
      aagaacgca caacatcgg gtttacaac cctacagcg 
      tacgaattt ctagagtac tacgagtcc ctcatcttt 
      catccccat cgattgagg tatttccgg caaaaagta 
      caacagcac agcaaacag gatattttt aaaacggag 
      ggagggcac acgtttttc atccccgtc gacgaggga 
      tttaagaac aagcgcgcc gaactaatc gacggcaaa 
      atcatcgac ggccacgtc atccccaag cacgtcctg 
      ttcaccagg cctacaaaa aaagacgtt cccttccaa 
      accctcgcc aacggagac aacaacgtt cgagttgtt 
      atttcgttc acgcaagaa acgcgaggc cccaataac 
      caagtcata aattacgtg aagagtcat actctgttg 
      agcgacggc aagcactcc cagggggtt gttttgtct 
      gaaatagtc aaagccaac atccccgtc aaaaacggg 
      gtggttcac ctcatccag aagcccttg atggtggtg 
      gataacaac gtgcgggag cttttggag gagaaagac 
      gggggcatt cttagcagc ttcgtgaaa gccatcaac 
      gacgccgat taccgggag ttttacaac acgctggag 
      cggtctcag gatatcacc ctgttcgcg ccttgcaac 
      aacgcctgg acggacgac ttcaccctc aacagcatc 
      atcagcaac aagaaccga tttaaggac atcctgatg 
      atgcacctg gtgcccgat aatcggctg tatgtcgac 
      aggatcctc agtattagc cacggcaga gacaagagg 
      gtgcacact cgcgacccc cgcaacgac ctctacttc 
      aatgtagta acatccggc accaaccgc accgtcacc 
      gtcgaagga ggcggcgtt aacgccact gtaattcaa 
      ccagacctc gcagccaca aatggtatc attcacgta 
      atcgataga gtgttggga gttccttat accacagtc 
      tacgacaag ttacgaacg gattattca ctcaactat 
      acgttccgt ctgggtaat caaggcggc ttcaacaac 
      caactcagc gataccacc aaaaagttt acttacttc 
      gttccccga aaccaggcc tgggacgaa gccagcgtg 
      gaccacccc tcagcaatc aaaaaactc ttcatgccg 
      gaatttcac taccacgcc actaacatc ctcgagcgc 
      cacctggtg atctccgac gagccctac accatggaa 
      cgcatcaaa caactaaca ctgaaatcc aaccaaaac 
      atgaacaac tacgagcga agaaccgaa atccaactc 
      ccgaccata cgcggcgaa cttaatcta tacgtggag 
      gagttgcaa gatcacact ttcataatc cactggaaa 
      ggcgaaaga atccccgta ttcaagcca aatgtagca 
      tgcaccaac ggagtcatc cacgtcatc gacgcccct 
      tttctgcaa gacggcgac atccgagtc agcggcgcc 
      ccatctctg cattttgcg ccccattta ataatgatt 
      ttggccgcc aaatggtta ttactttaa tggcgtccg 
      cttttttag tcatagact cgtgagaac ttaatacaa 
      tgtaacggt cggtctgtg aagattttc caaaaagtt 
      tgtttgatt atttggacc cagtttgcg ttctttcca 
      ttctttcag gcgggattc ttcacagac gccctgata 
      tatcaataa accacgttt tttgaataa tccataatc 
      aattacttg caccaatgc gacataatt tctatcata 
      ccttttaga ttgtattat ttacgattt gtaaataag 
      tcaattttt taattttgc atttgtata taggtagga 
      cccaaagtt agactactt taactcgga aattctaaa 
      ccaattggc cgcgatccc gatgaagca tttcagatt 
      atttccccc aaaaaactc taatcaatg ttgacgaaa 
      tcaaaatat ttattaaac aaactatgc cttctaatc 
      tgttgaata attttctaa aaattgttg actgaattg 
      aattttctc tatatcacg tttctaaat gtaaaaatg 
      aaatcattt cgtagtgcc cttaaactt atacattcc 
      actccaaca aactgttgc ttcactttg gttgaaccc 
      agctgtagg gggcgctca agtcggcag tcggtacct 
      gtaatccag cagcgcata tcctgtgtt tgcgccccc 
      ttccgggcg tatgattgg acgaattgc ttatttaaa 
      atgttgtaa atacgtgtg gatgtgtat aaatgttca 
      tatttttga cagtaaatg tttataaat gttagataa 
      ggaaaagtt ataggtagg ttttgcttt ccgtagtct 
      attataggt ctttgggca ctgaaatgc ttcacattt 
      aatgaaaat ctgacctcg agggtcgaa gcacaagtt 
      tttaaaacc ctcggtgtg agattttgt tagcgaacg 
      aaaccaaag aaatagccc attctgact gaaatgttt 
      ttaaaaact aattaatca atgtcaaat ataagtgta 
      taagaactg accgtacca tataataga tgtttaaag 
      cttaactat gttagggaa ctttcgaac gtttagact 
      ataaatgtg tgtgtaaga aaataattg cactgccaa 
      tgtgaacct aaattttac gtagtcgag ctgttttct 
      ttcacttga ccctcttcg tttcaactt gttttagct 
      aggattgta tataatatt ttctttcca ataaagcgt 
      tcagtgctc t
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