| General information | |||
| TC031362
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 19322166..19459665 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC031362 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01838 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC031362 RA
MGAQKCFSV GLVLAFSAF CVHSQSIDE KIKLDTDLS QFYNQLERS KLANVTLQY KQATIFAPI NEAFQRMEA TEANPDDLV LYHMTSIPR KTTQLGTSY TSMNSELEG NPPLWITHT TGNYHNDIY VNNARLLVS HSDIMTTQK NGNIQVVHK IDEVLVPTR SAKNAQHRV YNPTAYEFL EYYESLIFH PHRLRYFRQ KVQQHSKQD IFKTEGGHT FFIPVDEGF KNKRAELID GKIIDGHVI PKHVLFTRP TKKDVPFQT LANGDNNVR VVISFTQET RGPNNQVIN YVKSHTLLS DGKHSQGVV LSEIVKANI PVKNGVVHL IQKPLMVVD NNVRELLEE KDGGILSSF VKAINDADY REFYNTLER SQDITLFAP CNNAWTDDF TLNSIISNK NRFKDILMM HLVPDNRLY VDRILSISH GRDKRVHTR DPRNDLYFN VVTSGTNRT VTVEGGGVN ATVIQPDLA ATNGIIHVI DRVLGVPYT TVYDKLRTD YSLNYTFRL GNQGGFNNQ LSDTTKKFT YFVPRNQAW DEASVDHPS AIKKLFMPE FHYHATNIL ERHLVISDE PYTMERIKQ LTLKSNQNM NNYERRTEI QLPTIRGEL NLYVEELQD HTFIIHWKG ERIPVFKPN VACTNGVIH VIDAPFLQD GDIRVSGAP SLHFAPHLI MILAAKWLL L
- >TC031362 RB
MGAQKCFSV GLVLAFSAF CVHSQSIDE KIKLDTDLS QFKYENSFS YNQVRMWYV LGAIMGGQK CFFLGLLIT FSGFGVHLC FSPSLIEKL TLDVEFAQF YNQLERSKL ANVTLQYKQ ATIFAPINE AFQRMEATE ANPDDLVLY HMTSIPRKT TQLGTSYTS MNSELEGNP PLWITHTTG NYHNDIYVN NARLLVSHS DIMTTQKNG NIQVVHKID EVLVPTRSA KNAQHRVYN PTAYEFLEY YESLIFHPH RLRYFRQKV QQHSKQDIF KTEGGHTFF IPVDEGFKN KRAELIDGK IIDGHVIPK HVLFTRPTK KDVPFQTLA NGDNNVRVV ISFTQETRG PNNQVINYV KSHTLLSDG KHSQGVVLS EIVKANIPV KNGVVHLIQ KPLMVVDNN VRELLEEKD GGILSSFVK AINDADYRE FYNTLERSQ DITLFAPCN NAWTDDFTL NSIISNKNR FKDILMMHL VPDNRLYVD RILSISHGR DKRVHTRDP RNDLYFNVV TSGTNRTVT VEGGGVNAT VIQPDLAAT NGIIHVIDR VLGVPYTTV YDKLRTDYS LNYTFRLGN QGGFNNQLS DTTKKFTYF VPRNQAWDE ASVDHPSAI KKLFMPEFH YHATNILER HLVISDEPY TMERIKQLT LKSNQNMNN YERRTEIQL PTIRGELNL YVEELQDHT FIIHWKGER IPVFKPNVA CTNGVIHVI DAPFLQDGD IRVSGAPSL HFAPHLIMI LAAKWLLL
- >TC031362 RA
atgggggcg caaaagtgc ttttccgtg ggacttgtt ctagctttc tcggctttt tgcgtccac tcgcagtcc atcgacgag aaaatcaag ctagatacg gatttgtct cagttttac aaccaattg gagcgcagt aagctggcc aatgtgacc ctccaatac aagcaagcg acaatcttt gcccccata aacgaggcc ttccaaaga atggaagct acggaggca aacccggac gacctagtt ctctaccac atgacttca atcccgcgt aagaccacc caactgggc acctcctac acgtccatg aactccgag ctggaggga aatccccca ctttggatc acacacaca acggggaat taccacaac gatatctac gtgaacaat gcccgactc cttgtcagc cattcggat atcatgacg acgcagaag aatggcaac attcaggtt gtacataaa attgacgag gtcttggtt cccacgaga tcagccaag aacgcacaa catcgggtt tacaaccct acagcgtac gaatttcta gagtactac gagtccctc atctttcat ccccatcga ttgaggtat ttccggcaa aaagtacaa cagcacagc aaacaggat atttttaaa acggaggga gggcacacg tttttcatc cccgtcgac gagggattt aagaacaag cgcgccgaa ctaatcgac ggcaaaatc atcgacggc cacgtcatc cccaagcac gtcctgttc accaggcct acaaaaaaa gacgttccc ttccaaacc ctcgccaac ggagacaac aacgttcga gttgttatt tcgttcacg caagaaacg cgaggcccc aataaccaa gtcataaat tacgtgaag agtcatact ctgttgagc gacggcaag cactcccag ggggttgtt ttgtctgaa atagtcaaa gccaacatc cccgtcaaa aacggggtg gttcacctc atccagaag cccttgatg gtggtggat aacaacgtg cgggagctt ttggaggag aaagacggg ggcattctt agcagcttc gtgaaagcc atcaacgac gccgattac cgggagttt tacaacacg ctggagcgg tctcaggat atcaccctg ttcgcgcct tgcaacaac gcctggacg gacgacttc accctcaac agcatcatc agcaacaag aaccgattt aaggacatc ctgatgatg cacctggtg cccgataat cggctgtat gtcgacagg atcctcagt attagccac ggcagagac aagagggtg cacactcgc gacccccgc aacgacctc tacttcaat gtagtaaca tccggcacc aaccgcacc gtcaccgtc gaaggaggc ggcgttaac gccactgta attcaacca gacctcgca gccacaaat ggtatcatt cacgtaatc gatagagtg ttgggagtt ccttatacc acagtctac gacaagtta cgaacggat tattcactc aactatacg ttccgtctg ggtaatcaa ggcggcttc aacaaccaa ctcagcgat accaccaaa aagtttact tacttcgtt ccccgaaac caggcctgg gacgaagcc agcgtggac cacccctca gcaatcaaa aaactcttc atgccggaa tttcactac cacgccact aacatcctc gagcgccac ctggtgatc tccgacgag ccctacacc atggaacgc atcaaacaa ctaacactg aaatccaac caaaacatg aacaactac gagcgaaga accgaaatc caactcccg accatacgc ggcgaactt aatctatac gtggaggag ttgcaagat cacactttc ataatccac tggaaaggc gaaagaatc cccgtattc aagccaaat gtagcatgc accaacgga gtcatccac gtcatcgac gcccctttt ctgcaagac ggcgacatc cgagtcagc ggcgcccca tctctgcat tttgcgccc catttaata atgattttg gccgccaaa tggttatta ctttaa
- >TC031362 RB
atgggggcg caaaagtgc ttttccgtg ggacttgtt ctagctttc tcggctttt tgcgtccac tcgcagtcc atcgacgag aaaatcaag ctagatacg gatttgtct cagtttaaa tatgagaat tcgtttagt tacaaccaa gtacgaatg tggtacgtt cttggtgca ataatgggt gggcaaaag tgctttttc ctcggactt ttaataact ttttcgggt tttggcgtc catttgtgc ttttcgccg tctctcatc gagaaactt acattggat gttgagttt gctcagttt tacaaccaa ttggagcgc agtaagctg gccaatgtg accctccaa tacaagcaa gcgacaatc tttgccccc ataaacgag gccttccaa agaatggaa gctacggag gcaaacccg gacgaccta gttctctac cacatgact tcaatcccg cgtaagacc acccaactg ggcacctcc tacacgtcc atgaactcc gagctggag ggaaatccc ccactttgg atcacacac acaacgggg aattaccac aacgatatc tacgtgaac aatgcccga ctccttgtc agccattcg gatatcatg acgacgcag aagaatggc aacattcag gttgtacat aaaattgac gaggtcttg gttcccacg agatcagcc aagaacgca caacatcgg gtttacaac cctacagcg tacgaattt ctagagtac tacgagtcc ctcatcttt catccccat cgattgagg tatttccgg caaaaagta caacagcac agcaaacag gatattttt aaaacggag ggagggcac acgtttttc atccccgtc gacgaggga tttaagaac aagcgcgcc gaactaatc gacggcaaa atcatcgac ggccacgtc atccccaag cacgtcctg ttcaccagg cctacaaaa aaagacgtt cccttccaa accctcgcc aacggagac aacaacgtt cgagttgtt atttcgttc acgcaagaa acgcgaggc cccaataac caagtcata aattacgtg aagagtcat actctgttg agcgacggc aagcactcc cagggggtt gttttgtct gaaatagtc aaagccaac atccccgtc aaaaacggg gtggttcac ctcatccag aagcccttg atggtggtg gataacaac gtgcgggag cttttggag gagaaagac gggggcatt cttagcagc ttcgtgaaa gccatcaac gacgccgat taccgggag ttttacaac acgctggag cggtctcag gatatcacc ctgttcgcg ccttgcaac aacgcctgg acggacgac ttcaccctc aacagcatc atcagcaac aagaaccga tttaaggac atcctgatg atgcacctg gtgcccgat aatcggctg tatgtcgac aggatcctc agtattagc cacggcaga gacaagagg gtgcacact cgcgacccc cgcaacgac ctctacttc aatgtagta acatccggc accaaccgc accgtcacc gtcgaagga ggcggcgtt aacgccact gtaattcaa ccagacctc gcagccaca aatggtatc attcacgta atcgataga gtgttggga gttccttat accacagtc tacgacaag ttacgaacg gattattca ctcaactat acgttccgt ctgggtaat caaggcggc ttcaacaac caactcagc gataccacc aaaaagttt acttacttc gttccccga aaccaggcc tgggacgaa gccagcgtg gaccacccc tcagcaatc aaaaaactc ttcatgccg gaatttcac taccacgcc actaacatc ctcgagcgc cacctggtg atctccgac gagccctac accatggaa cgcatcaaa caactaaca ctgaaatcc aaccaaaac atgaacaac tacgagcga agaaccgaa atccaactc ccgaccata cgcggcgaa cttaatcta tacgtggag gagttgcaa gatcacact ttcataatc cactggaaa ggcgaaaga atccccgta ttcaagcca aatgtagca tgcaccaac ggagtcatc cacgtcatc gacgcccct tttctgcaa gacggcgac atccgagtc agcggcgcc ccatctctg cattttgcg ccccattta ataatgatt ttggccgcc aaatggtta ttactttaa
- >TC031362 RA
attcagggc agaattgaa tgttttggg ttttttcga aggagagtg ttatttttc aggtcgtaa acgccgcat gggtctgct tccccgcaa ctatatggt gggttatct gacctaatc ttacgtctt tcgcttctg aagccacat gcagtgtca gtccagccg tgcttgagt cagtcttcg ctaggaaaa aattaagct aagtttaat tatgtcaga ctatatatt ttctttatg tgtcgttat tttatagtt ttaacgaga tattttatc gagttctcc tctgtcaag gtttgttgt ttatctaga gatatagca aagtattgg atactaaaa accaaacag attgattat ttagtttat cactgccaa aagttcgac aattgcttt tagtaatgt aaaaaccct acttgtgat aaacttatt ggaggcgaa ctaaatgta ttttaagac agttgtaac ctattttaa tataatttt tagtattta cgatttttc tgtaaatat tgtttgcat tgtggtagc ttaatgcat catctaaga ctaaaaatc atcacatgg agcaaggtt gtgacttac ggaccaaac gaatcaaaa ttgtgctcc cgctttcct caactcatt cacattttc aggacttcg cacttgcat taaatgtag ccgtagtat tgtaattta cataaaata aaatgttga acaactgga atcgaccac ttttttcat cctcctcac gacactttg acccgccca cgtcgctgt cggtttctt gtagggtgt gtgtgattc cgtcttctc ttgagtgtc aacgaacta cgagtgtgg tgcgttttt ggtgcaata atgggggcg caaaagtgc ttttccgtg ggacttgtt ctagctttc tcggctttt tgcgtccac tcgcagtcc atcgacgag aaaatcaag ctagatacg gatttgtct cagttttac aaccaattg gagcgcagt aagctggcc aatgtgacc ctccaatac aagcaagcg acaatcttt gcccccata aacgaggcc ttccaaaga atggaagct acggaggca aacccggac gacctagtt ctctaccac atgacttca atcccgcgt aagaccacc caactgggc acctcctac acgtccatg aactccgag ctggaggga aatccccca ctttggatc acacacaca acggggaat taccacaac gatatctac gtgaacaat gcccgactc cttgtcagc cattcggat atcatgacg acgcagaag aatggcaac attcaggtt gtacataaa attgacgag gtcttggtt cccacgaga tcagccaag aacgcacaa catcgggtt tacaaccct acagcgtac gaatttcta gagtactac gagtccctc atctttcat ccccatcga ttgaggtat ttccggcaa aaagtacaa cagcacagc aaacaggat atttttaaa acggaggga gggcacacg tttttcatc cccgtcgac gagggattt aagaacaag cgcgccgaa ctaatcgac ggcaaaatc atcgacggc cacgtcatc cccaagcac gtcctgttc accaggcct acaaaaaaa gacgttccc ttccaaacc ctcgccaac ggagacaac aacgttcga gttgttatt tcgttcacg caagaaacg cgaggcccc aataaccaa gtcataaat tacgtgaag agtcatact ctgttgagc gacggcaag cactcccag ggggttgtt ttgtctgaa atagtcaaa gccaacatc cccgtcaaa aacggggtg gttcacctc atccagaag cccttgatg gtggtggat aacaacgtg cgggagctt ttggaggag aaagacggg ggcattctt agcagcttc gtgaaagcc atcaacgac gccgattac cgggagttt tacaacacg ctggagcgg tctcaggat atcaccctg ttcgcgcct tgcaacaac gcctggacg gacgacttc accctcaac agcatcatc agcaacaag aaccgattt aaggacatc ctgatgatg cacctggtg cccgataat cggctgtat gtcgacagg atcctcagt attagccac ggcagagac aagagggtg cacactcgc gacccccgc aacgacctc tacttcaat gtagtaaca tccggcacc aaccgcacc gtcaccgtc gaaggaggc ggcgttaac gccactgta attcaacca gacctcgca gccacaaat ggtatcatt cacgtaatc gatagagtg ttgggagtt ccttatacc acagtctac gacaagtta cgaacggat tattcactc aactatacg ttccgtctg ggtaatcaa ggcggcttc aacaaccaa ctcagcgat accaccaaa aagtttact tacttcgtt ccccgaaac caggcctgg gacgaagcc agcgtggac cacccctca gcaatcaaa aaactcttc atgccggaa tttcactac cacgccact aacatcctc gagcgccac ctggtgatc tccgacgag ccctacacc atggaacgc atcaaacaa ctaacactg aaatccaac caaaacatg aacaactac gagcgaaga accgaaatc caactcccg accatacgc ggcgaactt aatctatac gtggaggag ttgcaagat cacactttc ataatccac tggaaaggc gaaagaatc cccgtattc aagccaaat gtagcatgc accaacgga gtcatccac gtcatcgac gcccctttt ctgcaagac ggcgacatc cgagtcagc ggcgcccca tctctgcat tttgcgccc catttaata atgattttg gccgccaaa tggttatta ctttaatgg cgtccgctt ttttagtca tagactcgt gagaactta atacaatgt aacggtcgg tctgtgaag attttccaa aaagtttgt ttgattatt tggacccag tttgcgttc tttccattc tttcaggcg ggattcttc acagacgcc ctgatatat caataaacc acgtttttt gaataatcc ataatcaat tacttgcac caatgcgac ataatttct atcatacct tttagattg tattattta cgatttgta aataagtca attttttaa ttttgcatt tgtatatag gtaggaccc aaagttaga ctactttaa ctcggaaat tctaaacca attggccgc gatcccgat gaagcattt cagattatt tcccccaaa aaactctaa tcaatgttg acgaaatca aaatattta ttaaacaaa ctatgcctt ctaatctgt tgaataatt ttctaaaaa ttgttgact gaattgaat tttctctat atcacgttt ctaaatgta aaaatgaaa tcatttcgt agtgccctt aaacttata cattccact ccaacaaac tgttgcttc actttggtt gaacccagc tgtaggggg cgctcaagt cggcagtcg gtacctgta atccagcag cgcatatcc tgtgtttgc gcccccttc cgggcgtat gattggacg aattgctta tttaaaatg ttgtaaata cgtgtggat gtgtataaa tgttcatat ttttgacag taaatgttt ataaatgtt agataagga aaagttata ggtaggttt tgctttccg tagtctatt ataggtctt tgggcactg aaatgcttc acatttaat gaaaatctg acctcgagg gtcgaagca caagttttt aaaaccctc ggtgtgaga ttttgttag cgaacgaaa ccaaagaaa tagcccatt ctgactgaa atgttttta aaaactaat taatcaatg tcaaatata agtgtataa gaactgacc gtaccatat aatagatgt ttaaagctt aactatgtt agggaactt tcgaacgtt tagactata aatgtgtgt gtaagaaaa taattgcac tgccaatgt gaacctaaa ttttacgta gtcgagctg ttttctttc acttgaccc tcttcgttt caacttgtt ttagctagg attgtatat aatattttc tttccaata aagcgttca gtgctct
- >TC031362 RB
attcagggc agaattgaa tgttttggg ttttttcga aggagagtg ttatttttc aggtcgtaa acgccgcat gggtctgct tccccgcaa ctatatggt gggttatct gacctaatc ttacgtctt tcgcttctg aagccacat gcagtgtca gtccagccg tgcttgagt cagtcttcg ctaggaaaa aattaagct aagtttaat tatgtcaga ctatatatt ttctttatg tgtcgttat tttatagtt ttaacgaga tattttatc gagttctcc tctgtcaag gtttgttgt ttatctaga gatatagca aagtattgg atactaaaa accaaacag attgattat ttagtttat cactgccaa aagttcgac aattgcttt tagtaatgt aaaaaccct acttgtgat aaacttatt ggaggcgaa ctaaatgta ttttaagac agttgtaac ctattttaa tataatttt tagtattta cgatttttc tgtaaatat tgtttgcat tgtggtagc ttaatgcat catctaaga ctaaaaatc atcacatgg agcaaggtt gtgacttac ggaccaaac gaatcaaaa ttgtgctcc cgctttcct caactcatt cacattttc aggacttcg cacttgcat taaatgtag ccgtagtat tgtaattta cataaaata aaatgttga acaactgga atcgaccac ttttttcat cctcctcac gacactttg acccgccca cgtcgctgt cggtttctt gtagggtgt gtgtgattc cgtcttctc ttgagtgtc aacgaacta cgagtgtgg tgcgttttt ggtgcaata atgggggcg caaaagtgc ttttccgtg ggacttgtt ctagctttc tcggctttt tgcgtccac tcgcagtcc atcgacgag aaaatcaag ctagatacg gatttgtct cagtttaaa tatgagaat tcgtttagt tacaaccaa gtacgaatg tggtacgtt cttggtgca ataatgggt gggcaaaag tgctttttc ctcggactt ttaataact ttttcgggt tttggcgtc catttgtgc ttttcgccg tctctcatc gagaaactt acattggat gttgagttt gctcagttt tacaaccaa ttggagcgc agtaagctg gccaatgtg accctccaa tacaagcaa gcgacaatc tttgccccc ataaacgag gccttccaa agaatggaa gctacggag gcaaacccg gacgaccta gttctctac cacatgact tcaatcccg cgtaagacc acccaactg ggcacctcc tacacgtcc atgaactcc gagctggag ggaaatccc ccactttgg atcacacac acaacgggg aattaccac aacgatatc tacgtgaac aatgcccga ctccttgtc agccattcg gatatcatg acgacgcag aagaatggc aacattcag gttgtacat aaaattgac gaggtcttg gttcccacg agatcagcc aagaacgca caacatcgg gtttacaac cctacagcg tacgaattt ctagagtac tacgagtcc ctcatcttt catccccat cgattgagg tatttccgg caaaaagta caacagcac agcaaacag gatattttt aaaacggag ggagggcac acgtttttc atccccgtc gacgaggga tttaagaac aagcgcgcc gaactaatc gacggcaaa atcatcgac ggccacgtc atccccaag cacgtcctg ttcaccagg cctacaaaa aaagacgtt cccttccaa accctcgcc aacggagac aacaacgtt cgagttgtt atttcgttc acgcaagaa acgcgaggc cccaataac caagtcata aattacgtg aagagtcat actctgttg agcgacggc aagcactcc cagggggtt gttttgtct gaaatagtc aaagccaac atccccgtc aaaaacggg gtggttcac ctcatccag aagcccttg atggtggtg gataacaac gtgcgggag cttttggag gagaaagac gggggcatt cttagcagc ttcgtgaaa gccatcaac gacgccgat taccgggag ttttacaac acgctggag cggtctcag gatatcacc ctgttcgcg ccttgcaac aacgcctgg acggacgac ttcaccctc aacagcatc atcagcaac aagaaccga tttaaggac atcctgatg atgcacctg gtgcccgat aatcggctg tatgtcgac aggatcctc agtattagc cacggcaga gacaagagg gtgcacact cgcgacccc cgcaacgac ctctacttc aatgtagta acatccggc accaaccgc accgtcacc gtcgaagga ggcggcgtt aacgccact gtaattcaa ccagacctc gcagccaca aatggtatc attcacgta atcgataga gtgttggga gttccttat accacagtc tacgacaag ttacgaacg gattattca ctcaactat acgttccgt ctgggtaat caaggcggc ttcaacaac caactcagc gataccacc aaaaagttt acttacttc gttccccga aaccaggcc tgggacgaa gccagcgtg gaccacccc tcagcaatc aaaaaactc ttcatgccg gaatttcac taccacgcc actaacatc ctcgagcgc cacctggtg atctccgac gagccctac accatggaa cgcatcaaa caactaaca ctgaaatcc aaccaaaac atgaacaac tacgagcga agaaccgaa atccaactc ccgaccata cgcggcgaa cttaatcta tacgtggag gagttgcaa gatcacact ttcataatc cactggaaa ggcgaaaga atccccgta ttcaagcca aatgtagca tgcaccaac ggagtcatc cacgtcatc gacgcccct tttctgcaa gacggcgac atccgagtc agcggcgcc ccatctctg cattttgcg ccccattta ataatgatt ttggccgcc aaatggtta ttactttaa tggcgtccg cttttttag tcatagact cgtgagaac ttaatacaa tgtaacggt cggtctgtg aagattttc caaaaagtt tgtttgatt atttggacc cagtttgcg ttctttcca ttctttcag gcgggattc ttcacagac gccctgata tatcaataa accacgttt tttgaataa tccataatc aattacttg caccaatgc gacataatt tctatcata ccttttaga ttgtattat ttacgattt gtaaataag tcaattttt taattttgc atttgtata taggtagga cccaaagtt agactactt taactcgga aattctaaa ccaattggc cgcgatccc gatgaagca tttcagatt atttccccc aaaaaactc taatcaatg ttgacgaaa tcaaaatat ttattaaac aaactatgc cttctaatc tgttgaata attttctaa aaattgttg actgaattg aattttctc tatatcacg tttctaaat gtaaaaatg aaatcattt cgtagtgcc cttaaactt atacattcc actccaaca aactgttgc ttcactttg gttgaaccc agctgtagg gggcgctca agtcggcag tcggtacct gtaatccag cagcgcata tcctgtgtt tgcgccccc ttccgggcg tatgattgg acgaattgc ttatttaaa atgttgtaa atacgtgtg gatgtgtat aaatgttca tatttttga cagtaaatg tttataaat gttagataa ggaaaagtt ataggtagg ttttgcttt ccgtagtct attataggt ctttgggca ctgaaatgc ttcacattt aatgaaaat ctgacctcg agggtcgaa gcacaagtt tttaaaacc ctcggtgtg agattttgt tagcgaacg aaaccaaag aaatagccc attctgact gaaatgttt ttaaaaact aattaatca atgtcaaat ataagtgta taagaactg accgtacca tataataga tgtttaaag cttaactat gttagggaa ctttcgaac gtttagact ataaatgtg tgtgtaaga aaataattg cactgccaa tgtgaacct aaattttac gtagtcgag ctgttttct ttcacttga ccctcttcg tttcaactt gttttagct aggattgta tataatatt ttctttcca ataaagcgt tcagtgctc t
