Status
Please wait ...
Gene information
General information
TC011281 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 1904502..1905969
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011281
    iBeetle screen iB_01836

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    posterior terminus number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011281 RA
      MFALSSKNV LIVFLWIDY LWVQYLRAR QHKKTKVTT 
      RVPDELALS QQSFDKSRK QTLQRNRLA FVKDLVSII 
      TTTAIIQYK ILPTIWEET DPLGELDEI TRSCMWYFF 
      YTTFLAFIN LPFTIYDSI ILETSKSPE FVIWNQLKN 
      FVVGQIFAV MLCSLLITL IRNGDQVFI TFWLLFCLV 
      VFVVGISYP QMAPSKYRQ LSPLKPGNL RNEITNLAL 
      TLSFPLKEI YIEERFSKK SCSNIYFYG PSDQKSIVI 
      LNTLILKEH GIGCTNNQI LALISFEFS RWHFNETFK 
      YVIVLETNL LLSFAAFLF LFKHPQVYE IFGFEDFHP 
      VLVGVYVVL KYVMVPYAS LLSFGFMWV SRGFVMQND 
      EFVAQLGKG KALIEALVR LEENNVKFP VCDRLYSMW 
      HYDKPCLME RVEAIKKVE EEKFE
    CDS sequence
    • >TC011281 RA
      atgtttgca ctcagctcg aaaaatgtc ttgattgtg 
      tttttgtgg atcgactac ttgtgggtg cagtatttg 
      cgagcgaga caacacaag aaaactaaa gtcacaacg 
      agagttcca gacgagtta gcactctcg caacaatca 
      tttgataaa tccaggaaa caaaccctt caacgcaat 
      cgactcgct ttcgtcaaa gacttggtc tcaattatc 
      acaaccacc gcaatcatc cagtataaa attttacca 
      acaatatgg gaggaaacc gacccattg ggcgaacta 
      gacgaaatc actaggagt tgcatgtgg tattttttc 
      tacaccact ttcctggca ttcatcaat ttgccgttc 
      acaatctac gacagcata attctcgaa acgagcaaa 
      agcccggaa tttgtgatt tggaaccag ctcaagaat 
      tttgtagtt ggacagatt tttgcggtc atgctttgc 
      tcacttctt atcacactg attaggaac ggcgaccag 
      gtttttatc actttttgg ctactcttc tgcctagtt 
      gtgtttgta gtggggata agttacccc caaatggcc 
      ccctcaaag taccgccag ttgtccccc cttaaacca 
      ggcaacttg cggaacgaa atcacaaac ctagccttg 
      accttgagc tttccgctg aaagaaatc tacatcgag 
      gagagattc agcaaaaaa tcgtgctca aacatctat 
      ttttacggc ccatctgac caaaaatcc atcgttatt 
      ttgaacact ctcatcctg aaggaacac ggaattggg 
      tgcacgaat aaccaaatc ctggcttta atttcgttc 
      gaattttcc cgttggcac ttcaacgaa acgttcaaa 
      tatgtcata gtcctcgaa actaacctt ttgttgtcc 
      tttgctgct ttcctcttc ttgttcaag caccctcaa 
      gtttatgaa attttcggt tttgaggat tttcaccca 
      gttcttgtg ggtgtttac gtggttttg aagtacgtg 
      atggtgcct tacgcttcg ttgctcagt tttgggttt 
      atgtgggtt tcgagaggg tttgtgatg caaaatgat 
      gaatttgtg gcacagttg gggaaagga aaggctcta 
      attgaagct ttggttaga ttggaggaa aataacgtc 
      aagtttccg gtctgtgac cggttgtat tccatgtgg 
      cattacgac aagccatgt ctgatggag agggttgaa 
      gcgattaag aaggtggag gaggagaaa tttgagtag
    mRNA sequence
    • >TC011281 RA
      aaatggaag agacacccg tgtaaatgc atcataaaa 
      actaagaca tggtaatcg ttgagaagg taaacaagg 
      gatgattga gcaaaatgt caaacaaat ttccgtgtc 
      atttgtgtc aaaatgttt gcactcagc tcgaaaaat 
      gtcttgatt gtgtttttg tggatcgac tacttgtgg 
      gtgcagtat ttgcgagcg agacaacac aagaaaact 
      aaagtcaca acgagagtt ccagacgag ttagcactc 
      tcgcaacaa tcatttgat aaatccagg aaacaaacc 
      cttcaacgc aatcgactc gctttcgtc aaagacttg 
      gtctcaatt atcacaacc accgcaatc atccagtat 
      aaaatttta ccaacaata tgggaggaa accgaccca 
      ttgggcgaa ctagacgaa atcactagg agttgcatg 
      tggtatttt ttctacacc actttcctg gcattcatc 
      aatttgccg ttcacaatc tacgacagc ataattctc 
      gaaacgagc aaaagcccg gaatttgtg atttggaac 
      cagctcaag aattttgta gttggacag atttttgcg 
      gtcatgctt tgctcactt cttatcaca ctgattagg 
      aacggcgac caggttttt atcactttt tggctactc 
      ttctgccta gttgtgttt gtagtgggg ataagttac 
      ccccaaatg gccccctca aagtaccgc cagttgtcc 
      ccccttaaa ccaggcaac ttgcggaac gaaatcaca 
      aacctagcc ttgaccttg agctttccg ctgaaagaa 
      atctacatc gaggagaga ttcagcaaa aaatcgtgc 
      tcaaacatc tatttttac ggcccatct gaccaaaaa 
      tccatcgtt attttgaac actctcatc ctgaaggaa 
      cacggaatt gggtgcacg aataaccaa atcctggct 
      ttaatttcg ttcgaattt tcccgttgg cacttcaac 
      gaaacgttc aaatatgtc atagtcctc gaaactaac 
      cttttgttg tcctttgct gctttcctc ttcttgttc 
      aagcaccct caagtttat gaaattttc ggttttgag 
      gattttcac ccagttctt gtgggtgtt tacgtggtt 
      ttgaagtac gtgatggtg ccttacgct tcgttgctc 
      agttttggg tttatgtgg gtttcgaga gggtttgtg 
      atgcaaaat gatgaattt gtggcacag ttggggaaa 
      ggaaaggct ctaattgaa gctttggtt agattggag 
      gaaaataac gtcaagttt ccggtctgt gaccggttg 
      tattccatg tggcattac gacaagcca tgtctgatg 
      gagagggtt gaagcgatt aagaaggtg gaggaggag 
      aaatttgag tagcgttga gaattttga agtaaataa 
      aaaaattgt gaaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY