Status
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Gene information
General information
TC011262 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 1139395..1163730
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011262
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01835

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    egg chamber not present - Number of animals showing the phenotype: 4
    vitellarium mostly not present - Number of animals showing the phenotype: 4
    tropharium structure irregular - Number of animals showing the phenotype: 4

    Comment: cells of tropharia appear bigger than usual
    tropharium size decreased - Number of animals showing the phenotype: 4
    tropharium shape irregular - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number no/almost no eggs -
    ovariole mostly not present - Number of animals showing the phenotype: 4

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011262 RA
      MSISDLFGL DSPRGLLDF ANSSTNNGL CTPPVNASN 
      LQLNFDETS KFAPISTPS KYAMNLSLD SSSPTSSGY 
      YSGASLAST SPGSDFCGN SSPGVIERS IFNSSNSRS 
      DSPDSDSST VSNVEGKLS DLLNTLTLN DPLAKNLYK 
      NTDVDLQTL QLCSLQALK YLRPSNVLG SLLPPACSH 
      AAPHVLDKW NQTSPLATI PETFQLDRA ARFHRSAAA 
      VCDATCTWS GVLPPRTSK PSGYSSKVF LGGVPWDIS 
      EQSLIQAFK QFGPIRVEW PGKEKQISQ PKGYVYIIF 
      ESEKNVRAL LQACTSDYA NSGNWYYKI SSKRMKAKE 
      VQVIPWILN DSNCTKSTT QKLDPSKTV FVGALHGML 
      NAEGLAKIM NDLFDGVVY TGIDTDKYK YPIGSGRVT 
      FNNPRSYMK AVAAAFIEI KTSKFSKKV QVDPYLEDS 
      LCSVCGVQQ GPYFCRDIL CFRYFCRTC WQWQHNNGL 
      RHHKPLTRN SKNSPGLGM TNPN
    CDS sequence
    • >TC011262 RA
      atgtctatc agcgaccta ttcggctta gattccccg 
      aggggcttg ttggatttt gcaaattca tcgacaaat 
      aatggactc tgcacgcca ccagtcaac gcctcaaat 
      ttgcagttg aattttgac gaaacttca aagttcgca 
      ccgataagt acgcccagc aagtacgcc atgaacctg 
      tcattggat agctcatcg ccgacttcc tccgggtat 
      tattccggg gcttctttg gccagtact tcacctggg 
      agtgatttt tgtggtaat agcagtcct ggggttatc 
      gaacgctcc attttcaat tcgtccaat tcacgatct 
      gattcgccg gattcggat tcgagtact gtttcaaat 
      gttgagggg aagctgtcc gacttattg aacacctta 
      actttgaat gaccctttg gccaaaaat ttgtacaag 
      aataccgat gtggatttg cagaccctt caattgtgc 
      agtcttcaa gctttgaag tacttgcgt ccttctaat 
      gttttgggg tctctttta ccacctgct tgctctcat 
      gctgctccg catgtgttg gataagtgg aaccaaact 
      tcccctttg gccaccata cctgaaaca ttccagctt 
      gacagagcc gctcgtttt catcgttcg gcagctgct 
      gtgtgtgat gccacatgc acttggagc ggtgtctta 
      cctccacgt acttcaaaa ccttctggt tattccagc 
      aaagtattt ttgggtgga gttccatgg gatatttcc 
      gaacagtct ctcatccaa gcattcaaa caatttgga 
      ccgattcgt gtcgaatgg ccaggcaag gagaagcaa 
      atctctcag ccaaaagga tacgtttat attattttc 
      gagtcggag aagaacgtc cgagcttta ctacaagct 
      tgtacaagc gactatgcc aattctggt aattggtat 
      tacaagatt agttcgaag cgtatgaag gctaaagag 
      gtgcaagta attccgtgg atcctcaac gattcaaac 
      tgtaccaaa tcgacaact caaaagttg gacccgagc 
      aagactgtg ttcgttgga gctttacat ggaatgttg 
      aatgctgaa ggtttggcg aaaataatg aatgatttg 
      tttgacggt gttgtttac accggaata gatactgac 
      aaatacaag tatccgatc ggatccgga cgagttact 
      tttaacaat cctcggtcg tatatgaaa gctgtcgct 
      gcggctttt attgagatt aagacatcg aaattcagc 
      aaaaaggtg caagtggat ccgtacttg gaagactcc 
      ctgtgttcg gtgtgtggc gtccagcaa gggccttac 
      ttctgtcgc gacatctta tgcttccgt tacttttgc 
      cgcacctgc tggcagtgg cagcacaat aacggatta 
      cgccaccac aagccgttg acgaggaat tcaaaaaac 
      agtcctgga ttgggaatg accaatcca aattaa
    mRNA sequence
    • >TC011262 RA
      ttaatcgct tcaattcca ggaaactca ctctgctgt 
      tgacttaca tcgctaaat tagcagttg tctcgaagt 
      gatgtgaag tgttaaggg tgctctggt gggtgataa 
      ggtggtcga tctgcggtg tatttgcaa cacgtggtt 
      gtttgctat accgggata atgccggtg ctcgacagg 
      atggaggtg gctttgctt tcagataag tcggttttt 
      aacaagcaa acgatcact tgttttgaa gtttgaaac 
      gcgttaata attgtttta acaagtaat tttgtgatt 
      aaataacaa gatttcgtt tcaattaga tgacacgat 
      gtctatcag cgacctatt cggcttaga ttccccgag 
      gggcttgtt ggattttgc aaattcatc gacaaataa 
      tggactctg cacgccacc agtcaacgc ctcaaattt 
      gcagttgaa ttttgacga aacttcaaa gttcgcacc 
      gataagtac gcccagcaa gtacgccat gaacctgtc 
      attggatag ctcatcgcc gacttcctc cgggtatta 
      ttccggggc ttctttggc cagtacttc acctgggag 
      tgatttttg tggtaatag cagtcctgg ggttatcga 
      acgctccat tttcaattc gtccaattc acgatctga 
      ttcgccgga ttcggattc gagtactgt ttcaaatgt 
      tgaggggaa gctgtccga cttattgaa caccttaac 
      tttgaatga ccctttggc caaaaattt gtacaagaa 
      taccgatgt ggatttgca gacccttca attgtgcag 
      tcttcaagc tttgaagta cttgcgtcc ttctaatgt 
      tttggggtc tcttttacc acctgcttg ctctcatgc 
      tgctccgca tgtgttgga taagtggaa ccaaacttc 
      ccctttggc caccatacc tgaaacatt ccagcttga 
      cagagccgc tcgttttca tcgttcggc agctgctgt 
      gtgtgatgc cacatgcac ttggagcgg tgtcttacc 
      tccacgtac ttcaaaacc ttctggtta ttccagcaa 
      agtattttt gggtggagt tccatggga tatttccga 
      acagtctct catccaagc attcaaaca atttggacc 
      gattcgtgt cgaatggcc aggcaagga gaagcaaat 
      ctctcagcc aaaaggata cgtttatat tattttcga 
      gtcggagaa gaacgtccg agctttact acaagcttg 
      tacaagcga ctatgccaa ttctggtaa ttggtatta 
      caagattag ttcgaagcg tatgaaggc taaagaggt 
      gcaagtaat tccgtggat cctcaacga ttcaaactg 
      taccaaatc gacaactca aaagttgga cccgagcaa 
      gactgtgtt cgttggagc tttacatgg aatgttgaa 
      tgctgaagg tttggcgaa aataatgaa tgatttgtt 
      tgacggtgt tgtttacac cggaataga tactgacaa 
      atacaagta tccgatcgg atccggacg agttacttt 
      taacaatcc tcggtcgta tatgaaagc tgtcgctgc 
      ggcttttat tgagattaa gacatcgaa attcagcaa 
      aaaggtgca agtggatcc gtacttgga agactccct 
      gtgttcggt gtgtggcgt ccagcaagg gccttactt 
      ctgtcgcga catcttatg cttccgtta cttttgccg 
      cacctgctg gcagtggca gcacaataa cggattacg 
      ccaccacaa gccgttgac gaggaattc aaaaaacag 
      tcctggatt gggaatgac caatccaaa ttaaacgac 
      tattcacct tcgatttta ttctgatat agttgtgta 
      aggaaatgc cagtcgtac acggcgtct taaaattgc 
      cttttccat tatataatc ttagatgta ggaaatgtt 
      acattgttg ttgttttta gtgtaggtt ctttaaaga 
      tttggctat ttaaaaaga aaacaaaaa aatgataat 
      attggtagc ccgcgagag ttacgtaac tctgcaaaa 
      ttatctgtt actagtttt atacagctt cttatttct 
      gatttcgtt ttaacgcaa aatgtgaaa cttaacact 
      atgaaagct aactcattt taatgatgg catttggag 
      acagatttt acagtatta tcgaaaaaa aatccaagt 
      agctaacac cagtgtttt ataatagat ttgaattct 
      tgttcagtg tttaaacta atagtttta agcgtttga 
      taaataatt ttaaatcat aattgtgct aatttaact 
      ctaaattta ggtgggtag ttgggtaag attattaca 
      gtgctaaaa ctgtaaatt ggactgatt cggtattag 
      gaaatgcga cgttttttg gatacgatt tttggctac 
      cgatatatt tcagtactt atcagtttt ttaagctat 
      tgtttccat tacagttat taaatatca tgcataact 
      cattcgact ttttttcac gcctcagaa tacaatttt 
      ttgtttttt gttcttgtt accagtgtt tgtttgtga 
      tcgtagcat gtaagactt ttttaattg agttggtaa 
      gattttttt aattgttaa taattacct gataaattt 
      taccaactt gacgttcct ccaagggtg ttactaaca 
      ttactaatc cagagactg aaatcgccc taatttaag 
      ctgacggcg atttcgtct tgtttttac tgccttgcg 
      cattttaat ttttagtaa catttttaa ccaccaatt 
      acagtggcg cacaatgaa atatagatg tagcatttt 
      ttaaatttt aaagtaact cggttacat tttaacgct 
      ccgaataat tcaactgcc atattttaa taccatcta 
      tgttttatg tgtttcaac agttttatg tgaattggt 
      gggtgtttg tcacaactc gttttattt ttcctttgt 
      gttctcgtc tagtcccaa tgtattcca agtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY