General information | |||
TC011262 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007425.3: 1139395..1163730 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC011262 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01835 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)vitellarium mostly not present - Number of animals showing the phenotype: 4
tropharium structure irregular - Number of animals showing the phenotype: 4
Comment: cells of tropharia appear bigger than usual |
tropharium shape irregular - Number of animals showing the phenotype: 4
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
ovariole mostly not present - Number of animals showing the phenotype: 4
- >TC011262 RA
MSISDLFGL DSPRGLLDF ANSSTNNGL CTPPVNASN LQLNFDETS KFAPISTPS KYAMNLSLD SSSPTSSGY YSGASLAST SPGSDFCGN SSPGVIERS IFNSSNSRS DSPDSDSST VSNVEGKLS DLLNTLTLN DPLAKNLYK NTDVDLQTL QLCSLQALK YLRPSNVLG SLLPPACSH AAPHVLDKW NQTSPLATI PETFQLDRA ARFHRSAAA VCDATCTWS GVLPPRTSK PSGYSSKVF LGGVPWDIS EQSLIQAFK QFGPIRVEW PGKEKQISQ PKGYVYIIF ESEKNVRAL LQACTSDYA NSGNWYYKI SSKRMKAKE VQVIPWILN DSNCTKSTT QKLDPSKTV FVGALHGML NAEGLAKIM NDLFDGVVY TGIDTDKYK YPIGSGRVT FNNPRSYMK AVAAAFIEI KTSKFSKKV QVDPYLEDS LCSVCGVQQ GPYFCRDIL CFRYFCRTC WQWQHNNGL RHHKPLTRN SKNSPGLGM TNPN
- >TC011262 RA
atgtctatc agcgaccta ttcggctta gattccccg aggggcttg ttggatttt gcaaattca tcgacaaat aatggactc tgcacgcca ccagtcaac gcctcaaat ttgcagttg aattttgac gaaacttca aagttcgca ccgataagt acgcccagc aagtacgcc atgaacctg tcattggat agctcatcg ccgacttcc tccgggtat tattccggg gcttctttg gccagtact tcacctggg agtgatttt tgtggtaat agcagtcct ggggttatc gaacgctcc attttcaat tcgtccaat tcacgatct gattcgccg gattcggat tcgagtact gtttcaaat gttgagggg aagctgtcc gacttattg aacacctta actttgaat gaccctttg gccaaaaat ttgtacaag aataccgat gtggatttg cagaccctt caattgtgc agtcttcaa gctttgaag tacttgcgt ccttctaat gttttgggg tctctttta ccacctgct tgctctcat gctgctccg catgtgttg gataagtgg aaccaaact tcccctttg gccaccata cctgaaaca ttccagctt gacagagcc gctcgtttt catcgttcg gcagctgct gtgtgtgat gccacatgc acttggagc ggtgtctta cctccacgt acttcaaaa ccttctggt tattccagc aaagtattt ttgggtgga gttccatgg gatatttcc gaacagtct ctcatccaa gcattcaaa caatttgga ccgattcgt gtcgaatgg ccaggcaag gagaagcaa atctctcag ccaaaagga tacgtttat attattttc gagtcggag aagaacgtc cgagcttta ctacaagct tgtacaagc gactatgcc aattctggt aattggtat tacaagatt agttcgaag cgtatgaag gctaaagag gtgcaagta attccgtgg atcctcaac gattcaaac tgtaccaaa tcgacaact caaaagttg gacccgagc aagactgtg ttcgttgga gctttacat ggaatgttg aatgctgaa ggtttggcg aaaataatg aatgatttg tttgacggt gttgtttac accggaata gatactgac aaatacaag tatccgatc ggatccgga cgagttact tttaacaat cctcggtcg tatatgaaa gctgtcgct gcggctttt attgagatt aagacatcg aaattcagc aaaaaggtg caagtggat ccgtacttg gaagactcc ctgtgttcg gtgtgtggc gtccagcaa gggccttac ttctgtcgc gacatctta tgcttccgt tacttttgc cgcacctgc tggcagtgg cagcacaat aacggatta cgccaccac aagccgttg acgaggaat tcaaaaaac agtcctgga ttgggaatg accaatcca aattaa
- >TC011262 RA
ttaatcgct tcaattcca ggaaactca ctctgctgt tgacttaca tcgctaaat tagcagttg tctcgaagt gatgtgaag tgttaaggg tgctctggt gggtgataa ggtggtcga tctgcggtg tatttgcaa cacgtggtt gtttgctat accgggata atgccggtg ctcgacagg atggaggtg gctttgctt tcagataag tcggttttt aacaagcaa acgatcact tgttttgaa gtttgaaac gcgttaata attgtttta acaagtaat tttgtgatt aaataacaa gatttcgtt tcaattaga tgacacgat gtctatcag cgacctatt cggcttaga ttccccgag gggcttgtt ggattttgc aaattcatc gacaaataa tggactctg cacgccacc agtcaacgc ctcaaattt gcagttgaa ttttgacga aacttcaaa gttcgcacc gataagtac gcccagcaa gtacgccat gaacctgtc attggatag ctcatcgcc gacttcctc cgggtatta ttccggggc ttctttggc cagtacttc acctgggag tgatttttg tggtaatag cagtcctgg ggttatcga acgctccat tttcaattc gtccaattc acgatctga ttcgccgga ttcggattc gagtactgt ttcaaatgt tgaggggaa gctgtccga cttattgaa caccttaac tttgaatga ccctttggc caaaaattt gtacaagaa taccgatgt ggatttgca gacccttca attgtgcag tcttcaagc tttgaagta cttgcgtcc ttctaatgt tttggggtc tcttttacc acctgcttg ctctcatgc tgctccgca tgtgttgga taagtggaa ccaaacttc ccctttggc caccatacc tgaaacatt ccagcttga cagagccgc tcgttttca tcgttcggc agctgctgt gtgtgatgc cacatgcac ttggagcgg tgtcttacc tccacgtac ttcaaaacc ttctggtta ttccagcaa agtattttt gggtggagt tccatggga tatttccga acagtctct catccaagc attcaaaca atttggacc gattcgtgt cgaatggcc aggcaagga gaagcaaat ctctcagcc aaaaggata cgtttatat tattttcga gtcggagaa gaacgtccg agctttact acaagcttg tacaagcga ctatgccaa ttctggtaa ttggtatta caagattag ttcgaagcg tatgaaggc taaagaggt gcaagtaat tccgtggat cctcaacga ttcaaactg taccaaatc gacaactca aaagttgga cccgagcaa gactgtgtt cgttggagc tttacatgg aatgttgaa tgctgaagg tttggcgaa aataatgaa tgatttgtt tgacggtgt tgtttacac cggaataga tactgacaa atacaagta tccgatcgg atccggacg agttacttt taacaatcc tcggtcgta tatgaaagc tgtcgctgc ggcttttat tgagattaa gacatcgaa attcagcaa aaaggtgca agtggatcc gtacttgga agactccct gtgttcggt gtgtggcgt ccagcaagg gccttactt ctgtcgcga catcttatg cttccgtta cttttgccg cacctgctg gcagtggca gcacaataa cggattacg ccaccacaa gccgttgac gaggaattc aaaaaacag tcctggatt gggaatgac caatccaaa ttaaacgac tattcacct tcgatttta ttctgatat agttgtgta aggaaatgc cagtcgtac acggcgtct taaaattgc cttttccat tatataatc ttagatgta ggaaatgtt acattgttg ttgttttta gtgtaggtt ctttaaaga tttggctat ttaaaaaga aaacaaaaa aatgataat attggtagc ccgcgagag ttacgtaac tctgcaaaa ttatctgtt actagtttt atacagctt cttatttct gatttcgtt ttaacgcaa aatgtgaaa cttaacact atgaaagct aactcattt taatgatgg catttggag acagatttt acagtatta tcgaaaaaa aatccaagt agctaacac cagtgtttt ataatagat ttgaattct tgttcagtg tttaaacta atagtttta agcgtttga taaataatt ttaaatcat aattgtgct aatttaact ctaaattta ggtgggtag ttgggtaag attattaca gtgctaaaa ctgtaaatt ggactgatt cggtattag gaaatgcga cgttttttg gatacgatt tttggctac cgatatatt tcagtactt atcagtttt ttaagctat tgtttccat tacagttat taaatatca tgcataact cattcgact ttttttcac gcctcagaa tacaatttt ttgtttttt gttcttgtt accagtgtt tgtttgtga tcgtagcat gtaagactt ttttaattg agttggtaa gattttttt aattgttaa taattacct gataaattt taccaactt gacgttcct ccaagggtg ttactaaca ttactaatc cagagactg aaatcgccc taatttaag ctgacggcg atttcgtct tgtttttac tgccttgcg cattttaat ttttagtaa catttttaa ccaccaatt acagtggcg cacaatgaa atatagatg tagcatttt ttaaatttt aaagtaact cggttacat tttaacgct ccgaataat tcaactgcc atattttaa taccatcta tgttttatg tgtttcaac agttttatg tgaattggt gggtgtttg tcacaactc gttttattt ttcctttgt gttctcgtc tagtcccaa tgtattcca agtt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
