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Gene information
General information
TC011262 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid69: 1050202..1074537
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011262
    iBeetle screen iB_01835
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Fertility
    egg chamber not present - Number of animals showing the phenotype: 4
    vitellarium mostly not present - Number of animals showing the phenotype: 4
    tropharium structure irregular - Number of animals showing the phenotype: 4

    Comment: cells of tropharia appear bigger than usual
    tropharium size decreased - Number of animals showing the phenotype: 4
    tropharium shape irregular - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number no/almost no eggs -
    ovariole mostly not present - Number of animals showing the phenotype: 4

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011262 RA
      MSISDLFGL DSPRGLLDF ANSSTNNGL CTPPVNASN 
LQLNFDETS KFAPISTPS KYAMNLSLD SSSPTSSGY 
YSGASLAST SPGSDFCGN SSPGVIERS IFNSSNSRS 
DSPDSDSST VSNVEGKLS DLLNTLTLN DPLAKNLYK 
NTDVDLQTL QLCSLQALK YLRPSNVLG SLLPPACSH 
AAPHVLDKW NQTSPLATI PETFQLDRA ARFHRSAAA 
VCDATCTWS GVLPPRTSK PSGYSSKVF LGGVPWDIS 
EQSLIQAFK QFGPIRVEW PGKEKQISQ PKGYVYIIF 
ESEKNVRAL LQACTSDYA NSGNWYYKI SSKRMKAKE 
VQVIPWILN DSNCTKSTT QKLDPSKTV FVGALHGML 
NAEGLAKIM NDLFDGVVY TGIDTDKYK YPIGSGRVT 
FNNPRSYMK AVAAAFIEI KTSKFSKKV QVDPYLEDS 
LCSVCGVQQ GPYFCRDIL CFRYFCRTC WQWQHNNGL 
RHHKPLTRN SKNSPGLGM TNPN
    CDS sequence
    • >TC011262 RA
      atgtctatc agcgaccta ttcggctta gattccccg 
aggggcttg ttggatttt gcaaattca tcgacaaat 
aatggactc tgcacgcca ccagtcaac gcctcaaat 
ttgcagttg aattttgac gaaacttca aagttcgca 
ccgataagt acgcccagc aagtacgcc atgaacctg 
tcattggat agctcatcg ccgacttcc tccgggtat 
tattccggg gcttctttg gccagtact tcacctggg 
agtgatttt tgtggtaat agcagtcct ggggttatc 
gaacgctcc attttcaat tcgtccaat tcacgatct 
gattcgccg gattcggat tcgagtact gtttcaaat 
gttgagggg aagctgtcc gacttattg aacacctta 
actttgaat gaccctttg gccaaaaat ttgtacaag 
aataccgat gtggatttg cagaccctt caattgtgc 
agtcttcaa gctttgaag tacttgcgt ccttctaat 
gttttgggg tctctttta ccacctgct tgctctcat 
gctgctccg catgtgttg gataagtgg aaccaaact 
tcccctttg gccaccata cctgaaaca ttccagctt 
gacagagcc gctcgtttt catcgttcg gcagctgct 
gtgtgtgat gccacatgc acttggagc ggtgtctta 
cctccacgt acttcaaaa ccttctggt tattccagc 
aaagtattt ttgggtgga gttccatgg gatatttcc 
gaacagtct ctcatccaa gcattcaaa caatttgga 
ccgattcgt gtcgaatgg ccaggcaag gagaagcaa 
atctctcag ccaaaagga tacgtttat attattttc 
gagtcggag aagaacgtc cgagcttta ctacaagct 
tgtacaagc gactatgcc aattctggt aattggtat 
tacaagatt agttcgaag cgtatgaag gctaaagag 
gtgcaagta attccgtgg atcctcaac gattcaaac 
tgtaccaaa tcgacaact caaaagttg gacccgagc 
aagactgtg ttcgttgga gctttacat ggaatgttg 
aatgctgaa ggtttggcg aaaataatg aatgatttg 
tttgacggt gttgtttac accggaata gatactgac 
aaatacaag tatccgatc ggatccgga cgagttact 
tttaacaat cctcggtcg tatatgaaa gctgtcgct 
gcggctttt attgagatt aagacatcg aaattcagc 
aaaaaggtg caagtggat ccgtacttg gaagactcc 
ctgtgttcg gtgtgtggc gtccagcaa gggccttac 
ttctgtcgc gacatctta tgcttccgt tacttttgc 
cgcacctgc tggcagtgg cagcacaat aacggatta 
cgccaccac aagccgttg acgaggaat tcaaaaaac 
agtcctgga ttgggaatg accaatcca aattaa
    mRNA sequence
    • >TC011262 RA
      ttaatcgct tcaattcca ggaaactca ctctgctgt 
tgacttaca tcgctaaat tagcagttg tctcgaagt 
gatgtgaag tgttaaggg tgctctggt gggtgataa 
ggtggtcga tctgcggtg tatttgcaa cacgtggtt 
gtttgctat accgggata atgccggtg ctcgacagg 
atggaggtg gctttgctt tcagataag tcggttttt 
aacaagcaa acgatcact tgttttgaa gtttgaaac 
gcgttaata attgtttta acaagtaat tttgtgatt 
aaataacaa gatttcgtt tcaattaga tgacacgat 
gtctatcag cgacctatt cggcttaga ttccccgag 
gggcttgtt ggattttgc aaattcatc gacaaataa 
tggactctg cacgccacc agtcaacgc ctcaaattt 
gcagttgaa ttttgacga aacttcaaa gttcgcacc 
gataagtac gcccagcaa gtacgccat gaacctgtc 
attggatag ctcatcgcc gacttcctc cgggtatta 
ttccggggc ttctttggc cagtacttc acctgggag 
tgatttttg tggtaatag cagtcctgg ggttatcga 
acgctccat tttcaattc gtccaattc acgatctga 
ttcgccgga ttcggattc gagtactgt ttcaaatgt 
tgaggggaa gctgtccga cttattgaa caccttaac 
tttgaatga ccctttggc caaaaattt gtacaagaa 
taccgatgt ggatttgca gacccttca attgtgcag 
tcttcaagc tttgaagta cttgcgtcc ttctaatgt 
tttggggtc tcttttacc acctgcttg ctctcatgc 
tgctccgca tgtgttgga taagtggaa ccaaacttc 
ccctttggc caccatacc tgaaacatt ccagcttga 
cagagccgc tcgttttca tcgttcggc agctgctgt 
gtgtgatgc cacatgcac ttggagcgg tgtcttacc 
tccacgtac ttcaaaacc ttctggtta ttccagcaa 
agtattttt gggtggagt tccatggga tatttccga 
acagtctct catccaagc attcaaaca atttggacc 
gattcgtgt cgaatggcc aggcaagga gaagcaaat 
ctctcagcc aaaaggata cgtttatat tattttcga 
gtcggagaa gaacgtccg agctttact acaagcttg 
tacaagcga ctatgccaa ttctggtaa ttggtatta 
caagattag ttcgaagcg tatgaaggc taaagaggt 
gcaagtaat tccgtggat cctcaacga ttcaaactg 
taccaaatc gacaactca aaagttgga cccgagcaa 
gactgtgtt cgttggagc tttacatgg aatgttgaa 
tgctgaagg tttggcgaa aataatgaa tgatttgtt 
tgacggtgt tgtttacac cggaataga tactgacaa 
atacaagta tccgatcgg atccggacg agttacttt 
taacaatcc tcggtcgta tatgaaagc tgtcgctgc 
ggcttttat tgagattaa gacatcgaa attcagcaa 
aaaggtgca agtggatcc gtacttgga agactccct 
gtgttcggt gtgtggcgt ccagcaagg gccttactt 
ctgtcgcga catcttatg cttccgtta cttttgccg 
cacctgctg gcagtggca gcacaataa cggattacg 
ccaccacaa gccgttgac gaggaattc aaaaaacag 
tcctggatt gggaatgac caatccaaa ttaaacgac 
tattcacct tcgatttta ttctgatat agttgtgta 
aggaaatgc cagtcgtac acggcgtct taaaattgc 
cttttccat tatataatc ttagatgta ggaaatgtt 
acattgttg ttgttttta gtgtaggtt ctttaaaga 
tttggctat ttaaaaaga aaacaaaaa aatgataat 
attggtagc ccgcgagag ttacgtaac tctgcaaaa 
ttatctgtt actagtttt atacagctt cttatttct 
gatttcgtt ttaacgcaa aatgtgaaa cttaacact 
atgaaagct aactcattt taatgatgg catttggag 
acagatttt acagtatta tcgaaaaaa aatccaagt 
agctaacac cagtgtttt ataatagat ttgaattct 
tgttcagtg tttaaacta atagtttta agcgtttga 
taaataatt ttaaatcat aattgtgct aatttaact 
ctaaattta ggtgggtag ttgggtaag attattaca 
gtgctaaaa ctgtaaatt ggactgatt cggtattag 
gaaatgcga cgttttttg gatacgatt tttggctac 
cgatatatt tcagtactt atcagtttt ttaagctat 
tgtttccat tacagttat taaatatca tgcataact 
cattcgact ttttttcac gcctcagaa tacaatttt 
ttgtttttt gttcttgtt accagtgtt tgtttgtga 
tcgtagcat gtaagactt ttttaattg agttggtaa 
gattttttt aattgttaa taattacct gataaattt 
taccaactt gacgttcct ccaagggtg ttactaaca 
ttactaatc cagagactg aaatcgccc taatttaag 
ctgacggcg atttcgtct tgtttttac tgccttgcg 
cattttaat ttttagtaa catttttaa ccaccaatt 
acagtggcg cacaatgaa atatagatg tagcatttt 
ttaaatttt aaagtaact cggttacat tttaacgct 
ccgaataat tcaactgcc atattttaa taccatcta 
tgttttatg tgtttcaac agttttatg tgaattggt 
gggtgtttg tcacaactc gttttattt ttcctttgt 
gttctcgtc tagtcccaa tgtattcca agtt