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Gene information
General information
TC011256 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 742745..754873
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011256
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01833

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011256 RA
      MENDPSKQE KAPEASFKK PLLVGKIGK FPKRLQNTE 
      STPNKVIEN KDSKEETEP STVAVPASD DVPPVHKPV 
      ESKKTEIKS LPYEEPNWS SLPETTSQD YVLEVLKNG 
      SIIETVNVM KKPFWTFGR LATCDICMQ HPTISRYHA 
      ILQYRKDDK NSGFYIYDL ESTHGTFLN KNRIKPRNY 
      VRMRVGHML KLGCSTRSY ILTGPEQDA EEESALSVT 
      ELKQKREEQ IRQRELEKL EEEERLRKQ EERGIDWGL 
      GEDADEETD LSENPFAQT NNEDLYLDN PKKALRGFF 
      EREGLELEY DCTEQGMGQ FLCKVELPV DDEMGRPIF 
      AEIFHKGKK KEAVVQCAL EACRILDRH GLLRQATHE 
      SRKRKPKNW EENDYYDSD DDTFLDRTG AIEKKREMR 
      IKAKVPQKA ETYESLMEK EKEINDSIK KLESELEEC 
      QKSLASVGT SSSGEADSL DSFMMELKQ SKPSKQSLN 
      KLKSELIKL KQEHGNVIK LVNIARPAS LPPLVPEYA 
      PNPNSGKVE KKSLPIFGK RKKIKVQVV SKSEPMHID 
      DGEEDEEEE SEKEQTAEQ EQVKEKEER HEEEQVEKL 
      QKTDLKKDV NVDTNERKK RRNQRRMQQ KQERAEIEK 
      KKGYDEDAY KEDYNMWVP PSGQSGDGR TSLNDKLGY
    CDS sequence
    • >TC011256 RA
      atggaaaac gacccttca aagcaagaa aaagcgccc 
      gaagcctcg ttcaagaag cccctgttg gtgggaaaa 
      attggcaaa tttccaaaa agattgcaa aatacggaa 
      tcaacacca aataaagtc attgaaaac aaagacagt 
      aaggaagaa acagagcca agtactgtt gcagttcca 
      gcctcagac gacgttccc cctgtacat aaaccagtc 
      gaaagtaag aaaacagaa attaagtca ttgccgtac 
      gaagagccc aactggtca tcgttacca gaaacgact 
      tcacaagac tatgttttg gaagtattg aaaaacggc 
      tcaattatt gagactgtg aatgtgatg aagaagccg 
      ttttggacg tttggacgc ctggccacg tgtgatatt 
      tgcatgcaa catccgacc atttccaga tatcatgcc 
      attttacaa tacagaaaa gatgacaaa aactcaggc 
      ttctacatt tacgatttg gaaagcaca catggcacg 
      tttttgaac aaaaatcga atcaaaccg aggaattac 
      gtacggatg cgggttggc cacatgtta aaacttgga 
      tgcagcacc agatcgtac attttaacc ggccctgaa 
      caagacgct gaggaagaa tcagcttta tctgtcacc 
      gaactgaag caaaaacgg gaggaacaa atccgtcag 
      cgtgagcta gaaaagtta gaagaagaa gaaaggtta 
      agaaaacaa gaagaacga ggcattgac tggggattg 
      ggtgaagac gcggacgaa gaaacggat ttatccgaa 
      aatcccttc gcccaaacg aataacgaa gacctatat 
      cttgacaat ccgaagaaa gcattgcgc ggtttcttc 
      gaaagagaa ggcttagag ctggaatat gattgcacg 
      gaacaagga atgggacaa tttctgtgc aaggttgag 
      cttccagtt gatgacgag atgggacgt ccaattttt 
      gccgaaatc ttccacaag ggtaagaag aaggaagct 
      gtggtccag tgtgctttg gaagcgtgt aggatacta 
      gatagacat ggtttgtta cgacaagcg acacatgag 
      tcacgcaaa aggaaacca aaaaattgg gaagaaaat 
      gattattac gatagtgac gatgacacg tttttggat 
      cgaactggt gccattgaa aagaagagg gaaatgagg 
      attaaggcg aaagtgcca caaaaagca gaaacttac 
      gaaagtttg atggagaag gagaaagaa atcaatgat 
      tctattaaa aaactagaa tcggaacta gaagaatgt 
      caaaaatct ctagcgtca gttggtact tcatctagt 
      ggtgaagcc gattcattg gactctttc atgatggaa 
      ttaaaacag tcgaaaccg agtaaacaa agtttgaat 
      aaacttaaa tctgagctt attaaactg aagcaagaa 
      catggaaat gttatcaaa ttggtaaat atagcacgg 
      cctgctagt ttaccacct ttggtacct gaatacgca 
      cctaatccc aacagtggt aaagtggag aagaagagt 
      ttaccgatt tttgggaag cgtaaaaaa attaaagtc 
      caggtggtt agtaagagt gaaccgatg catattgat 
      gatggtgaa gaagatgaa gaagaagaa agtgaaaaa 
      gaacaaacg gctgaacaa gaacaagtc aaagaaaaa 
      gaagagcgg catgaggag gaacaggtt gaaaaatta 
      caaaagact gacctgaag aaagacgta aatgtggac 
      acgaacgaa agaaagaaa agaagaaat caaagacgg 
      atgcaacag aaacaagaa cgtgccgaa attgagaag 
      aagaagggt tatgacgaa gatgcgtac aaggaggat 
      tataacatg tgggtacct cctagtggt cagtcgggg 
      gatggacgt acctctttg aatgataaa ttgggttat 
      taa
    mRNA sequence
    • >TC011256 RA
      tcggtggca caaaatcga tcgatactt gaataagcg 
      ttgttatat cttaagcac cttttgtat taattgtgt 
      cacgtgact tgagcattt gacagagat ttgacacat 
      tctaaccta acttttcac ccaaataaa caccatgga 
      aaacgaccc ttcaaagca agaaaaagc gcccgaagc 
      ctcgttcaa gaagcccct gttggtggg aaaaattgg 
      caaatttcc aaaaagatt gcaaaatac ggaatcaac 
      accaaataa agtcattga aaacaaaga cagtaagga 
      agaaacaga gccaagtac tgttgcagt tccagcctc 
      agacgacgt tccccctgt acataaacc agtcgaaag 
      taagaaaac agaaattaa gtcattgcc gtacgaaga 
      gcccaactg gtcatcgtt accagaaac gacttcaca 
      agactatgt tttggaagt attgaaaaa cggctcaat 
      tattgagac tgtgaatgt gatgaagaa gccgttttg 
      gacgtttgg acgcctggc cacgtgtga tatttgcat 
      gcaacatcc gaccatttc cagatatca tgccatttt 
      acaatacag aaaagatga caaaaactc aggcttcta 
      catttacga tttggaaag cacacatgg cacgttttt 
      gaacaaaaa tcgaatcaa accgaggaa ttacgtacg 
      gatgcgggt tggccacat gttaaaact tggatgcag 
      caccagatc gtacatttt aaccggccc tgaacaaga 
      cgctgagga agaatcagc tttatctgt caccgaact 
      gaagcaaaa acgggagga acaaatccg tcagcgtga 
      gctagaaaa gttagaaga agaagaaag gttaagaaa 
      acaagaaga acgaggcat tgactgggg attgggtga 
      agacgcgga cgaagaaac ggatttatc cgaaaatcc 
      cttcgccca aacgaataa cgaagacct atatcttga 
      caatccgaa gaaagcatt gcgcggttt cttcgaaag 
      agaaggctt agagctgga atatgattg cacggaaca 
      aggaatggg acaatttct gtgcaaggt tgagcttcc 
      agttgatga cgagatggg acgtccaat ttttgccga 
      aatcttcca caagggtaa gaagaagga agctgtggt 
      ccagtgtgc tttggaagc gtgtaggat actagatag 
      acatggttt gttacgaca agcgacaca tgagtcacg 
      caaaaggaa accaaaaaa ttgggaaga aaatgatta 
      ttacgatag tgacgatga cacgttttt ggatcgaac 
      tggtgccat tgaaaagaa gagggaaat gaggattaa 
      ggcgaaagt gccacaaaa agcagaaac ttacgaaag 
      tttgatgga gaaggagaa agaaatcaa tgattctat 
      taaaaaact agaatcgga actagaaga atgtcaaaa 
      atctctagc gtcagttgg tacttcatc tagtggtga 
      agccgattc attggactc tttcatgat ggaattaaa 
      acagtcgaa accgagtaa acaaagttt gaataaact 
      taaatctga gcttattaa actgaagca agaacatgg 
      aaatgttat caaattggt aaatatagc acggcctgc 
      tagtttacc acctttggt acctgaata cgcacctaa 
      tcccaacag tggtaaagt ggagaagaa gagtttacc 
      gatttttgg gaagcgtaa aaaaattaa agtccaggt 
      ggttagtaa gagtgaacc gatgcatat tgatgatgg 
      tgaagaaga tgaagaaga agaaagtga aaaagaaca 
      aacggctga acaagaaca agtcaaaga aaaagaaga 
      gcggcatga ggaggaaca ggttgaaaa attacaaaa 
      gactgacct gaagaaaga cgtaaatgt ggacacgaa 
      cgaaagaaa gaaaagaag aaatcaaag acggatgca 
      acagaaaca agaacgtgc cgaaattga gaagaagaa 
      gggttatga cgaagatgc gtacaagga ggattataa 
      catgtgggt acctcctag tggtcagtc gggggatgg 
      acgtacctc tttgaatga taaattggg ttattaata 
      aatttttat attttatct tgtgtttta ttcaatcac 
      ctacacaca caagtaaca gacaatgat ccaataatt 
      gtttttatt tttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY