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Gene information
General information
TC011254 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 712617..714718
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011254
    iBeetle screen iB_01832

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011254 RA
      MFKIPWIRT LQTRSMHLL KDKAYINGQ WLGAQSGKV 
      FQVTNPATG EVIGTAPDM DTSDTQRAI EGAHAAFQT 
      WQDTTAKDR SHYLRKWYD LMAQNHEDL AKIITLEAG 
      KPLVEARGE VAYGNSFIE FFSEEARRI HGEIIPSPI 
      PTKKILVER QPIGVAGLI APWNFPHAM ITRKAGAAL 
      ASGCTCVIK PAEDTPFTA LALMELAHQ AGIPSGVVN 
      VVTSSRENA PAIGNLLCE SPLVAGISF TGSTQVGKL 
      LYRQCSSGV KRLGLELGG NAPFIVFNS ANVGHAVKG 
      AMASKFRNC GQTCVSSNR FLVQGKVYD SFVGSLVKE 
      IKSLKIGNG QECGVNVGP LINQAQFGK VSDLVEDAV 
      SKGAKVLTG GKAARQFGE LFYEPTVLT DIKENMRVY 
      TEEVFGPIV TIFRFETEE EGLQIANST ERGLAGYFY 
      SEDVSQIFR VARKLEVGM CGVNEGIIS TAEAPFGGI 
      KESGLGREG SHHGIDDFT YIKYICVGN L
    CDS sequence
    • >TC011254 RA
      atgttcaaa attccctgg attcgaact ttgcaaacg 
      cgatcgatg cacctcctc aaagacaag gcttatatc 
      aacggccag tggctgggg gcccagtcg ggcaaagtc 
      ttccaagta accaacccc gcaacgggc gaagtcatc 
      ggcacggcc cccgacatg gacacctcc gacacgcag 
      agagccatc gagggggcc catgccgcc ttccaaacc 
      tggcaagac accaccgcc aaagaccgc tcccactac 
      ctccgtaaa tggtacgac ctaatggcc caaaaccac 
      gaagacctc gccaaaatc ataaccctg gaagcgggg 
      aagcccctg gtggaggcc cgcggcgaa gtcgcttac 
      ggcaactcc ttcatagag tttttctcc gaagaggcg 
      cgccgtatc cacggcgaa attattcca agccccatc 
      ccaaccaaa aaaatccta gtcgagaga caaccaatc 
      ggagtcgcc gggctcata gccccctgg aacttcccc 
      catgcgatg atcacacgc aaagcaggc gctgcgtta 
      gcctccggc tgcacttgc gtgatcaag ccggccgag 
      gacacaccc ttcacagcg cttgctttg atggaactg 
      gcccatcaa gcggggatt cccagtggg gttgttaac 
      gtggtgacc agcagtcgc gaaaatgcg ccagcaatc 
      gggaatttg ctatgcgag agccctcta gtggcgggg 
      atttcgttc acgggctcg acgcaagtc gggaaacta 
      ctttaccga cagtgttcc tctggtgtt aagagactg 
      gggcttgag ttaggtggc aatgccccg ttcattgtt 
      tttaattct gccaatgtt ggtcatgct gttaagggg 
      gccatggcc tccaagttt cggaattgt ggccaaacg 
      tgcgtctcc tctaaccgc tttctcgtc caaggcaaa 
      gtttacgat tcgttcgtt ggtagttta gttaaggaa 
      atcaaaagt ttgaagatt gggaatggg caagaatgt 
      ggggtgaat gtgggaccg ttgataaac caggcccag 
      tttggcaaa gtctcagat ttggtcgag gatgcggtt 
      agtaagggg gcgaaggtt ttgacaggg ggcaaagcc 
      gcaagacag tttggcgag ttgttttac gaaccaacc 
      gttttaacc gatattaag gaaaatatg agggtttac 
      acagaggag gtgtttggg cccattgtt accatattt 
      aggttcgag acggaagaa gagggcctc cagatcgcc 
      aatagtacc gagagaggt ctcgcgggt tatttctat 
      tccgaggat gtgtcccaa atttttagg gtggctagg 
      aaactcgaa gtggggatg tgtggggtt aatgaaggg 
      attatctca acggcggag gctcctttc ggagggatt 
      aaggagtcg gggctgggg agggaaggg tcccaccat 
      gggatcgac gattttacg tatattaag tacatttgc 
      gttggtaat ttatga
    mRNA sequence
    • >TC011254 RA
      ctgttctct aaaaataac tcaccattc attttagtg 
      gttatgtga ttatgttca tattaaaat gacattgtg 
      tgggtcatt cgtgttccc agtgtgtta aaccaataa 
      ttttcgacc cgaaacatc ccaaataac cgtaaaaaa 
      tgttcaaaa ttccctgga ttcgaactt tgcaaacgc 
      gatcgatgc acctcctca aagacaagg cttatatca 
      acggccagt ggctggggg cccagtcgg gcaaagtct 
      tccaagtaa ccaaccccg caacgggcg aagtcatcg 
      gcacggccc ccgacatgg acacctccg acacgcaga 
      gagccatcg agggggccc atgccgcct tccaaacct 
      ggcaagaca ccaccgcca aagaccgct cccactacc 
      tccgtaaat ggtacgacc taatggccc aaaaccacg 
      aagacctcg ccaaaatca taaccctgg aagcgggga 
      agcccctgg tggaggccc gcggcgaag tcgcttacg 
      gcaactcct tcatagagt ttttctccg aagaggcgc 
      gccgtatcc acggcgaaa ttattccaa gccccatcc 
      caaccaaaa aaatcctag tcgagagac aaccaatcg 
      gagtcgccg ggctcatag ccccctgga acttccccc 
      atgcgatga tcacacgca aagcaggcg ctgcgttag 
      cctccggct gcacttgcg tgatcaagc cggccgagg 
      acacaccct tcacagcgc ttgctttga tggaactgg 
      cccatcaag cggggattc ccagtgggg ttgttaacg 
      tggtgacca gcagtcgcg aaaatgcgc cagcaatcg 
      ggaatttgc tatgcgaga gccctctag tggcgggga 
      tttcgttca cgggctcga cgcaagtcg ggaaactac 
      tttaccgac agtgttcct ctggtgtta agagactgg 
      ggcttgagt taggtggca atgccccgt tcattgttt 
      ttaattctg ccaatgttg gtcatgctg ttaaggggg 
      ccatggcct ccaagtttc ggaattgtg gccaaacgt 
      gcgtctcct ctaaccgct ttctcgtcc aaggcaaag 
      tttacgatt cgttcgttg gtagtttag ttaaggaaa 
      tcaaaagtt tgaagattg ggaatgggc aagaatgtg 
      gggtgaatg tgggaccgt tgataaacc aggcccagt 
      ttggcaaag tctcagatt tggtcgagg atgcggtta 
      gtaaggggg cgaaggttt tgacagggg gcaaagccg 
      caagacagt ttggcgagt tgttttacg aaccaaccg 
      ttttaaccg atattaagg aaaatatga gggtttaca 
      cagaggagg tgtttgggc ccattgtta ccatattta 
      ggttcgaga cggaagaag agggcctcc agatcgcca 
      atagtaccg agagaggtc tcgcgggtt atttctatt 
      ccgaggatg tgtcccaaa tttttaggg tggctagga 
      aactcgaag tggggatgt gtggggtta atgaaggga 
      ttatctcaa cggcggagg ctcctttcg gagggatta 
      aggagtcgg ggctgggga gggaagggt cccaccatg 
      ggatcgacg attttacgt atattaagt acatttgcg 
      ttggtaatt tatgaaggg attaatccc ggtttaggt 
      tcgttttaa gaagcattc cataagtga aggaaagtg 
      ttaaagttt atttttcac tttttgtga atattattg 
      cagtgttaa tgaacttag ctgggctat ttccgcatt 
      gttaggtgt gcttctgcc aagttgtta aatttagtt 
      gtccaagtg tcaagagct aggtaccaa tatttttct 
      gtttagttt taggaaaaa aaagtcaag tagtgtgta 
      agtacaaca cgtataaag tcttccaga acaagtctt 
      ccgtcataa agtacatat attttagac ctccgataa 
      gtcaatatt aggtgctct tataattga ttttgtttg 
      tgatagtgt taggtggtt aataacaca tttttatct 
      ccttatagg tataccaaa ctgatttat tcttatctc 
      aacacaatg ttcctgttt gttttttat aataataaa 
      tgtgatata aataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY