Status
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Gene information
General information
TC031326
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011242,TC011243
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid69: 8954..26364
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031326
    iBeetle screen iB_01830
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031326 RA
      MATVPGFAN PDQTVEIND STEEKVKKK GSKKGGGFQ 
      CMGLSYNVL KGITKRGYQ QPTPIQRKT IPLVLEGRD 
      VVAMARTGS GKTACFLIP MFEKLKIRS AKAGARALI 
      LSPTRELAL QTLKFIKEL GRFTGLKSA VILGGDSMD 
      DQFSAIHGN PDIIVATPG RFLHVCIEM ELKLNSVEY 
      VVFDEADRL FEMGLGEQL TEIVNRLPD ARQTVLFSA 
      TLPKVLVEF AKAGLNDPV LLRLDVESK IPEELQLWY 
      IIVRPEERL AALLVLLKT HIDNKQQTV VFAATRHHV 
      EYIHMILDR VGVSNTYIY SNLDPSARK INAAKFSTG 
      KVKVLVVTD VAARGIDIP QLDNVINYN FPAKSKLFV 
      HRVGRCARA GRSGTAYSL VTPDEYPYL LDLHLFLGR 
      PLSIKTRTE NEGSVGKIP QALLEEQHS ALLTLHENN 
      IDLVSMKKV TENAYLQYV RSRPAASSD SNRRVKELA 
      FASCGIHPI FGDYKEYVE EERENLLEK MKSYRPQGT 
      IFEICGKNK SQEYQIMKQ KRSFHKEKI TSFHQNQEQ 
      KENEKQLLN HKDETTLAK SSSEDIDDA FDRVITPKK 
      RKLENLYKR NKKQKTKDE NFIPYLPAD RHTEEGLAI 
      NNFHNEASK VALDLTGDS AETMRKSVQ AKKWDRKKK 
      KMVAVPDKR SGKIKTESG VWIPATYKS NRYAQWKEK 
      SKIGQSNSD DENDETGPS GPKLHTEAK THWARHNKK 
      LQQKQRKSE LKTTDQILK ARKIAEKKK NRQKKKGKK 
      FDKANKMVV LNIPEVPKS EHTENYFSE FSNNTTIHG 
      FKYFVDRGI SVFEKIWWV LAFCLCAYV CTSMVMSNW 
      VKWDENPVF LSVSTKPMK HWEVPFPAI TICPENKIW 
      KTKYNYTYY KEKFKNRTL TLADYTFNG HFSHKNVLN 
      WDYDNNYAK VNKIGYPFR TTKITDSLT VQLNLDLNN 
      IDKNCDSSQ GYKVYAHHP AEMPSKYVK INNEKKYRF 
      EIKPEAIIS DSSLIDYNP QRRKCFYSY ERPLKFYKI 
      YTRRNCETE CLANFTLKS CGCIPYYMP FKEKSDLSE 
      CNCLPSCTS IKYDMDVST EESGTEIRQ MSNVMTDLT 
      FVFNDVEFI PMTRNELFG NADFLANLG GLLGLFCGF 
      SALSFFEIV YYLTLRWIC RICGLWTRI LASLGNISY
    CDS sequence
    • >TC031326 RA
      atggccacg gtccccggg ttcgcaaac ccggaccaa 
      accgtcgaa atcaatgac tcgactgaa gaaaaagta 
      aaaaagaag ggctcgaaa aaagggggt ggtttccaa 
      tgcatgggg ctcagttac aacgtcctg aagggcata 
      acgaagcga ggctaccaa cagccgacc ccaatccaa 
      cgcaaaaca attcctctc gtgctagaa gggcgagac 
      gtggtggca atggcacgc accggcagt ggcaaaact 
      gcctgtttc ctgatccca atgtttgag aaactgaaa 
      atacggagc gctaaggcg ggggccaga gctttgatt 
      ttgtcccca acgcgcgag ttggcctta caaacttta 
      aagtttatt aaagagctg gggcgcttt actggcttg 
      aaatcggcg gtgattttg ggtggggac tctatggat 
      gaccaattt tcggctatt cacggtaat cctgatata 
      attgtggcg acaccaggc aggtttttg cacgtctgt 
      attgagatg gagctcaag ttgaacagt gttgaatac 
      gttgttttt gacgaagct gatcggttg tttgagatg 
      gggcttggg gaacagttg actgagatt gtaaaccgg 
      ctgccggac gcaagacaa accgttttg ttttcggca 
      actttgccc aaagttttg gtcgagttt gccaaagct 
      ggattgaat gatcccgtt ttgttgcgt ttggatgtt 
      gagtcgaaa attccggaa gagttgcaa ttgtggtac 
      attattgtg agacctgag gaacgtctc gcagctttg 
      ctcgtgctt ttaaagaca catattgat aataaacaa 
      caaactgtg gtttttgcg gccacgaga caccacgtc 
      gagtacatt catatgatt ttggatcgt gtcggtgtt 
      agtaacact tatatttac tcaaatttg gatccttca 
      gctcgtaaa ataaatgcg gcgaaattt tccactggg 
      aaagttaag gttttagtg gtaactgat gtggcagct 
      cgtggtatt gatattccg cagttggat aatgtgata 
      aactacaat tttccggcc aagtcaaaa ttgtttgtc 
      catagggtt ggtcgatgt gcacgtgcc ggtcgctca 
      ggcacagcc tactccctg gtcacccct gacgaatac 
      ccctaccta ctcgatttg catttattt ttggggcgc 
      cctctatcc atcaaaact cgaaccgaa aacgagggt 
      tcggttgga aaaatccca caagcgtta cttgaagaa 
      caacatagt gccctttta acactacac gaaaacaac 
      atagatttg gtaagcatg aaaaaagtg accgaaaat 
      gcgtatttg caatacgtg cggtcacgg ccagctgcg 
      tcctcagac agtaaccga agagtcaaa gaattggcg 
      tttgcgagt tgtggaatt catccgatt tttggcgat 
      tataaggaa tatgtggag gaagaacgg gagaattta 
      ctggaaaaa atgaaaagt tataggcca caagggaca 
      atattcgag atctgtggg aaaaacaag tcgcaggaa 
      tatcaaatt atgaagcag aaaaggtcg tttcataaa 
      gaaaaaatc acaagtttt caccaaaat caggaacag 
      aaggaaaat gaaaaacaa ttattgaac cacaaggac 
      gaaacaact ttggctaaa agtagttca gaagatatt 
      gacgatgca tttgaccga gtcataaca cctaagaag 
      cgcaaattg gagaatttg tacaaaagg aataaaaaa 
      cagaaaacg aaagatgaa aattttatt ccctattta 
      cctgccgat cgacacact gaggaaggc ttggctatt 
      aataatttc cacaatgag gcaagtaaa gtcgcgtta 
      gatttaacc ggagattcg gcagaaacg atgcggaaa 
      agtgtacaa gcgaagaag tgggaccgg aaaaagaag 
      aaaatggtg gcagttccg gacaaacgg agtggtaaa 
      attaagaca gagtcaggt gtttggata ccagcaact 
      tacaaaagc aatcgctac gcgcaatgg aaggaaaaa 
      tcaaagatt ggccaaagc aacagtgat gatgaaaat 
      gacgaaaca ggtccttca ggtcccaaa ttgcacacc 
      gaagccaag acgcattgg gcccgccac aacaaaaaa 
      ctacaacag aagcaaaga aaatccgaa ttgaaaaca 
      accgatcaa atcctgaag gcgcgaaaa atcgccgaa 
      aagaaaaag aaccgacag aagaaaaaa ggcaagaaa 
      tttgataaa gccaacaag atggttgtt ctaaatata 
      ccggaagtc ccaaaaagt gagcatacc gaaaactac 
      ttttcggaa ttttccaac aatacgaca attcacggt 
      ttcaaatat ttcgtcgac cgtggcatt tccgtgttt 
      gaaaagatt tggtgggtt ttggccttt tgtttgtgt 
      gcttatgtt tgcacttct atggtcatg tcgaattgg 
      gtgaaatgg gacgaaaat ccagttttt ttgagcgtg 
      agtaccaaa ccgatgaag cattgggaa gttccattt 
      cctgccatt accatttgt cccgaaaat aaaatctgg 
      aaaacgaaa tataattac acatactat aaagaaaaa 
      ttcaaaaac agaactttg acacttgcc gattacacg 
      ttcaatggt catttcagc cataaaaac gtcctgaac 
      tgggattat gacaataat tatgccaaa gttaacaaa 
      atcgggtac ccatttcga accacaaaa atcaccgac 
      tctctcaca gttcaactg aacttggac ttaaacaat 
      attgataaa aattgcgac tcttctcag ggttacaaa 
      gtttatgcc caccatccg gcggaaatg ccctcaaaa 
      tacgtcaaa atcaacaac gagaaaaaa tacagattt 
      gagatcaaa cctgaggcc ataataagc gattccagt 
      ttaattgat tacaatcca cagagacgc aagtgtttc 
      tattcctac gaacggcct ttgaaattt tacaaaatt 
      tacacgcgt cggaattgc gagaccgaa tgtcttgcc 
      aatttcacg ctgaagtct tgcgggtgt attccttat 
      tacatgcca tttaaagaa aagtcggac ttgtctgaa 
      tgtaactgc ctgccgtct tgcacctcg attaagtac 
      gatatggac gtaagtact gaagagtct gggactgaa 
      attcggcaa atgagtaat gttatgacc gacttaacg 
      ttcgtattt aacgatgta gagtttatt ccaatgact 
      aggaatgaa ttatttgga aatgcggac tttttggcc 
      aatttgggc ggactttta gggcttttt tgtggattt 
      tctgctctg tcgtttttt gaaattgtt tattacttg 
      acgctgcgt tggatatgt cgcatttgc ggtctctgg 
      acgcgaata cttgcaagt ttgggaaat atttcttat 
      taa
    mRNA sequence
    • >TC031326 RA
      attttttaa aatatttag ccatggcca cggtccccg 
      ggttcgcaa acccggacc aaaccgtcg aaatcaatg 
      actcgactg aagaaaaag taaaaaaga agggctcga 
      aaaaagggg gtggtttcc aatgcatgg ggctcagtt 
      acaacgtcc tgaagggca taacgaagc gaggctacc 
      aacagccga ccccaatcc aacgcaaaa caattcctc 
      tcgtgctag aagggcgag acgtggtgg caatggcac 
      gcaccggca gtggcaaaa ctgcctgtt tcctgatcc 
      caatgtttg agaaactga aaatacgga gcgctaagg 
      cgggggcca gagctttga ttttgtccc caacgcgcg 
      agttggcct tacaaactt taaagttta ttaaagagc 
      tggggcgct ttactggct tgaaatcgg cggtgattt 
      tgggtgggg actctatgg atgaccaat tttcggcta 
      ttcacggta atcctgata taattgtgg cgacaccag 
      gcaggtttt tgcacgtct gtattgaga tggagctca 
      agttgaaca gtgttgaat acgttgttt ttgacgaag 
      ctgatcggt tgtttgaga tggggcttg gggaacagt 
      tgactgaga ttgtaaacc ggctgccgg acgcaagac 
      aaaccgttt tgttttcgg caactttgc ccaaagttt 
      tggtcgagt ttgccaaag ctggattga atgatcccg 
      ttttgttgc gtttggatg ttgagtcga aaattccgg 
      aagagttgc aattgtggt acattattg tgagacctg 
      aggaacgtc tcgcagctt tgctcgtgc ttttaaaga 
      cacatattg ataataaac aacaaactg tggtttttg 
      cggccacga gacaccacg tcgagtaca ttcatatga 
      ttttggatc gtgtcggtg ttagtaaca cttatattt 
      actcaaatt tggatcctt cagctcgta aaataaatg 
      cggcgaaat tttccactg ggaaagtta aggttttag 
      tggtaactg atgtggcag ctcgtggta ttgatattc 
      cgcagttgg ataatgtga taaactaca attttccgg 
      ccaagtcaa aattgtttg tccataggg ttggtcgat 
      gtgcacgtg ccggtcgct caggcacag cctactccc 
      tggtcaccc ctgacgaat acccctacc tactcgatt 
      tgcatttat ttttggggc gccctctat ccatcaaaa 
      ctcgaaccg aaaacgagg gttcggttg gaaaaatcc 
      cacaagcgt tacttgaag aacaacata gtgcccttt 
      taacactac acgaaaaca acatagatt tggtaagca 
      tgaaaaaag tgaccgaaa atgcgtatt tgcaatacg 
      tgcggtcac ggccagctg cgtcctcag acagtaacc 
      gaagagtca aagaattgg cgtttgcga gttgtggaa 
      ttcatccga tttttggcg attataagg aatatgtgg 
      aggaagaac gggagaatt tactggaaa aaatgaaaa 
      gttataggc cacaaggga caatattcg agatctgtg 
      ggaaaaaca agtcgcagg aatatcaaa ttatgaagc 
      agaaaaggt cgtttcata aagaaaaaa tcacaagtt 
      ttcaccaaa atcaggaac agaaggaaa atgaaaaac 
      aattattga accacaagg acgaaacaa ctttggcta 
      aaagtagtt cagaagata ttgacgatg catttgacc 
      gagtcataa cacctaaga agcgcaaat tggagaatt 
      tgtacaaaa ggaataaaa aacagaaaa cgaaagatg 
      aaaatttta ttccctatt tacctgccg atcgacaca 
      ctgaggaag gcttggcta ttaataatt tccacaatg 
      aggcaagta aagtcgcgt tagatttaa ccggagatt 
      cggcagaaa cgatgcgga aaagtgtac aagcgaaga 
      agtgggacc ggaaaaaga agaaaatgg tggcagttc 
      cggacaaac ggagtggta aaattaaga cagagtcag 
      gtgtttgga taccagcaa cttacaaaa gcaatcgct 
      acgcgcaat ggaaggaaa aatcaaaga ttggccaaa 
      gcaacagtg atgatgaaa atgacgaaa caggtcctt 
      caggtccca aattgcaca ccgaagcca agacgcatt 
      gggcccgcc acaacaaaa aactacaac agaagcaaa 
      gaaaatccg aattgaaaa caaccgatc aaatcctga 
      aggcgcgaa aaatcgccg aaaagaaaa agaaccgac 
      agaagaaaa aaggcaaga aatttgata aagccaaca 
      agatggttg ttctaaata taccggaag tcccaaaaa 
      gtgagcata ccgaaaact acttttcgg aattttcca 
      acaatacga caattcacg gtttcaaat atttcgtcg 
      accgtggca tttccgtgt ttgaaaaga tttggtggg 
      ttttggcct tttgtttgt gtgcttatg tttgcactt 
      ctatggtca tgtcgaatt gggtgaaat gggacgaaa 
      atccagttt ttttgagcg tgagtacca aaccgatga 
      agcattggg aagttccat ttcctgcca ttaccattt 
      gtcccgaaa ataaaatct ggaaaacga aatataatt 
      acacatact ataaagaaa aattcaaaa acagaactt 
      tgacacttg ccgattaca cgttcaatg gtcatttca 
      gccataaaa acgtcctga actgggatt atgacaata 
      attatgcca aagttaaca aaatcgggt acccatttc 
      gaaccacaa aaatcaccg actctctca cagttcaac 
      tgaacttgg acttaaaca atattgata aaaattgcg 
      actcttctc agggttaca aagtttatg cccaccatc 
      cggcggaaa tgccctcaa aatacgtca aaatcaaca 
      acgagaaaa aatacagat ttgagatca aacctgagg 
      ccataataa gcgattcca gtttaattg attacaatc 
      cacagagac gcaagtgtt tctattcct acgaacggc 
      ctttgaaat tttacaaaa tttacacgc gtcggaatt 
      gcgagaccg aatgtcttg ccaatttca cgctgaagt 
      cttgcgggt gtattcctt attacatgc catttaaag 
      aaaagtcgg acttgtctg aatgtaact gcctgccgt 
      cttgcacct cgattaagt acgatatgg acgtaagta 
      ctgaagagt ctgggactg aaattcggc aaatgagta 
      atgttatga ccgacttaa cgttcgtat ttaacgatg 
      tagagttta ttccaatga ctaggaatg aattatttg 
      gaaatgcgg actttttgg ccaatttgg gcggacttt 
      tagggcttt tttgtggat tttctgctc tgtcgtttt 
      ttgaaattg tttattact tgacgctgc gttggatat 
      gtcgcattt gcggtctct ggacgcgaa tacttgcaa 
      gtttgggaa atatttctt attaaataa taccgtgtt 
      tggagtggt tttggaatt cagttggga acacttaat 
      aatcaaatt aagaagcc