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Gene information
General information
TC011240 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 89317..93235
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011240
    iBeetle screen iB_01829

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011240 RA
      MGNDHSHLS GVEIEEKSV EVSDFWSHH SATIINSEN 
      TTNLSVLTG ELFVDGPLW KTQTPLEKN SKHLMIYRH 
      PCILRYISS WQKSSKYYL AVEEVVPLS HVLATQNAL 
      QICIGLHSI LKALCFLHE KASISHNNI CTAAIYASK 
      EGSWKLGGM EYVCKFNEL SSDFLKKTR NSRYNKAID 
      PNEDKHIQG PRRDFIDIF AFGVLVCEV LKSKDEEIP 
      GLTSFKELC KNDLQNIDV ALRPKLSSL LDHEFFNHE 
      FIVIHSFLA ELPLKSDEE KMQFFHSLA ERLKAFPEV 
      IVAKQLGNL LLSRLVLLN KTAQEELLP FILRLRVES 
      EDNTGNIFT EDVFRKFLT PKLLEIFGV RDAQIRLLL 
      LNNFKHFVH TFTKEELQS CILPELLVG VKDTNDHLV 
      SVTLRTLAD LVPILGAAT VIGGRRGKL FTDGRPVSH 
      PNRRTSRRN SKKIEPVIP PDTNPIIQT NQVIMDLPE 
      RPRPDGEEG ETSTEEAEQ SVEEDLDNW EDWGESNPQ 
      EIVESVESN SSESVTSVF ERENVAQKK LPDILELDI 
      KNQTSSGGN DDFDFFQDM EPVIETSAK FVIEEESAS 
      KLKLGVKED GNEDGWVDE DW
    CDS sequence
    • >TC011240 RA
      atgggcaat gaccactca catttaagc ggcgttgaa 
      atagaagaa aaatcagtc gaagtttca gacttctgg 
      tcacaccat tccgctact attatcaat tcagaaaac 
      acaacaaac ctctcagta cttacaggc gagttattc 
      gtcgatggc ccattatgg aaaacgcag acaccttta 
      gagaaaaat agcaagcat ttaatgatc tatcgtcac 
      ccctgcatt ttacgatac atatcatcg tggcaaaaa 
      agttccaag tattattta gccgtggag gaggttgtg 
      cccttaagc catgtcctg gcgacgcaa aatgcgctg 
      cagatttgt attgggtta cattccata ctgaaagct 
      ttgtgtttt ttgcatgag aaggcttca atctcgcat 
      aataatatc tgtactgcg gcgatttac gcaagtaag 
      gagggtagc tggaagctg ggaggcatg gaatacgtg 
      tgtaaattt aacgagtta agttccgat tttctcaaa 
      aaaacgaga aacagtcgg tataacaaa gccatcgat 
      cccaatgaa gacaaacac atacagggc cctagacgg 
      gactttatt gatattttt gcattcgga gttttagtt 
      tgtgaagtt ttaaagtca aaagatgag gagattcct 
      ggcctcaca agttttaaa gaattgtgc aagaacgat 
      ttgcaaaat attgacgtg gcgttgagg ccaaagctg 
      agttctctg ctcgaccac gaatttttt aatcatgag 
      tttattgtt atacattcg ttcctcgct gagctaccg 
      ttgaaatca gatgaggaa aaaatgcaa tttttccat 
      agtttagct gagaggctt aaagccttc cctgaagtt 
      atcgtagcc aaacaattg ggtaacttg ttactctct 
      aggttagtt cttttgaat aaaacggca caagaggag 
      ttgttacca tttatttta cgcctcaga gttgaaagt 
      gaagataat accggaaat attttcaca gaagatgta 
      tttaggaaa tttttgacc ccaaaattg ttggaaatt 
      tttggcgtt cgagacgcc caaattcgt ctcttgtta 
      ttaaacaat ttcaagcat tttgttcac acttttaca 
      aaggaagaa ctccaatct tgtatttta ccagagctt 
      ttggtgggc gttaaggac accaacgac cacttggtc 
      tcagtaacc ctgcgcacg ctcgccgac ttggtcccc 
      attttgggg gccgcgacc gtgataggg ggccgcagg 
      ggcaaactt tttaccgat ggccgcccc gtatcgcac 
      ccaaatcgg cgcacctct cgccgcaat tcgaaaaaa 
      atcgaaccg gtgattccc ccagacacc aatccgata 
      attcaaacc aatcaagtg attatggac ctccctgaa 
      cggccaagg cctgacggg gaagaaggg gagacctcc 
      acagaggag gcggaacaa tccgtggag gaggatttg 
      gataactgg gaggactgg ggggagtca aaccctcag 
      gaaattgtc gaatcagtt gaatcgaat tctagtgag 
      tctgtgacg tcggttttt gaacgcgaa aatgtggca 
      cagaaaaaa ttgcccgat attttggaa ctggatatt 
      aagaatcag acgagttcg gggggcaac gacgatttt 
      gatttcttc caagacatg gaacctgtg attgagact 
      tcggcgaag tttgtgatt gaggaggag agtgcgagt 
      aaattaaag ttgggggtg aaggaggat gggaatgag 
      gacggttgg gtcgatgaa gactggtag
    mRNA sequence
    • >TC011240 RA
      cccgccatc acttctgat tttgacagg tgacattca 
      aatgacgtt tcactaacc tctcaaaag tccaatctt 
      atttttttt attttccaa aatatgggc aatgaccac 
      tcacattta agcggcgtt gaaatagaa gaaaaatca 
      gtcgaagtt tcagacttc tggtcacac cattccgct 
      actattatc aattcagaa aacacaaca aacctctca 
      gtacttaca ggcgagtta ttcgtcgat ggcccatta 
      tggaaaacg cagacacct ttagagaaa aatagcaag 
      catttaatg atctatcgt cacccctgc attttacga 
      tacatatca tcgtggcaa aaaagttcc aagtattat 
      ttagccgtg gaggaggtt gtgccctta agccatgtc 
      ctggcgacg caaaatgcg ctgcagatt tgtattggg 
      ttacattcc atactgaaa gctttgtgt tttttgcat 
      gagaaggct tcaatctcg cataataat atctgtact 
      gcggcgatt tacgcaagt aaggagggt agctggaag 
      ctgggaggc atggaatac gtgtgtaaa tttaacgag 
      ttaagttcc gattttctc aaaaaaacg agaaacagt 
      cggtataac aaagccatc gatcccaat gaagacaaa 
      cacatacag ggccctaga cgggacttt attgatatt 
      tttgcattc ggagtttta gtttgtgaa gttttaaag 
      tcaaaagat gaggagatt cctggcctc acaagtttt 
      aaagaattg tgcaagaac gatttgcaa aatattgac 
      gtggcgttg aggccaaag ctgagttct ctgctcgac 
      cacgaattt tttaatcat gagtttatt gttatacat 
      tcgttcctc gctgagcta ccgttgaaa tcagatgag 
      gaaaaaatg caatttttc catagttta gctgagagg 
      cttaaagcc ttccctgaa gttatcgta gccaaacaa 
      ttgggtaac ttgttactc tctaggtta gttcttttg 
      aataaaacg gcacaagag gagttgtta ccatttatt 
      ttacgcctc agagttgaa agtgaagat aataccgga 
      aatattttc acagaagat gtatttagg aaatttttg 
      accccaaaa ttgttggaa atttttggc gttcgagac 
      gcccaaatt cgtctcttg ttattaaac aatttcaag 
      cattttgtt cacactttt acaaaggaa gaactccaa 
      tcttgtatt ttaccagag cttttggtg ggcgttaag 
      gacaccaac gaccacttg gtctcagta accctgcgc 
      acgctcgcc gacttggtc cccattttg ggggccgcg 
      accgtgata gggggccgc aggggcaaa ctttttacc 
      gatggccgc cccgtatcg cacccaaat cggcgcacc 
      tctcgccgc aattcgaaa aaaatcgaa ccggtgatt 
      cccccagac accaatccg ataattcaa accaatcaa 
      gtgattatg gacctccct gaacggcca aggcctgac 
      ggggaagaa ggggagacc tccacagag gaggcggaa 
      caatccgtg gaggaggat ttggataac tgggaggac 
      tggggggag tcaaaccct caggaaatt gtcgaatca 
      gttgaatcg aattctagt gagtctgtg acgtcggtt 
      tttgaacgc gaaaatgtg gcacagaaa aaattgccc 
      gatattttg gaactggat attaagaat cagacgagt 
      tcggggggc aacgacgat tttgatttc ttccaagac 
      atggaacct gtgattgag acttcggcg aagtttgtg 
      attgaggag gagagtgcg agtaaatta aagttgggg 
      gtgaaggag gatgggaat gaggacggt tgggtcgat 
      gaagactgg tagtggggt tgatttatt gcaattata 
      tctatgtta tgtaaatat acgtacttt taattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY