Status
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Gene information
General information
TC011211 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 2112664..2114511
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011211
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01827

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011211 RA
      MLTKLLLLA ILCQSFAEV YENTFDCED HFSESHENG 
TIEYHNFVT TNVKFYLYT PPPDNDTIE LNSTNLHLV 
NLTQPTKII IHGLQSYEN QVWVDKMVQ NYHTKGDYN 
IIAVDWSVI SSDETFYVV SAVDDLGRY VGDFIMEVT 
QDNYQYLSD VHLIGSSLG AQIAGAAGS AISNKTNAR 
VDRITGLDP TQPENLQLL DENTAEFVD VIHTTLYYG 
QESCGTVDF YVMAEDPQR PICYNDTCV ELLGDDYYA 
VTIIDEDRF EGVTCYNTS LAVQRLCDK AKSAVMGEY 
VPRDVTGSF LVKDSAGVI YSGRRVLFS IISTLLLLR 
LIV
    CDS sequence
    • >TC011211 RA
      atgttgaca aaacttctc ctcctcgcc attctctgc 
caaagcttt gctgaagtc tacgagaac acttttgac 
tgcgaagac catttcagc gaaagccac gaaaatggt 
accatagag taccataac ttcgtcacc acgaacgtg 
aaattctac ctttacact cctccaccc gacaatgac 
accatcgag ctgaattcg accaactta cacttggtc 
aatttgacc caacccacg aaaatcatc atccatggt 
ctccagtcg tacgaaaac caggtatgg gtggacaaa 
atggtacag aactaccat acaaagggc gattacaac 
ataatcgcg gtggattgg tccgttatc tcctcggac 
gaaactttc tacgttgtt tccgccgtg gatgacctc 
gggcggtac gtgggggac ttcatcatg gaggtcacc 
caggacaac taccagtac ctctctgac gtccacttg 
atcggctcc tcactaggt gcgcaaatc gcaggggct 
gctggaagc gcaatttcc aacaaaacc aacgccaga 
gttgaccga ataaccggc ctggaccca actcagccg 
gaaaacttg cagcttttg gacgagaac actgcagag 
tttgtcgat gttatccat acgacgctc tactatggc 
caggagagt tgtgggacg gttgatttt tatgtcatg 
gctgaggat ccacaaaga cccatttgt tataacgat 
acttgtgta gagctttta ggggatgac tattacgcg 
gtcactatc atcgatgag gatagattt gaaggggtt 
acatgctat aatacctct ctagccgtt cagagattg 
tgtgataag gccaaatcg gctgttatg ggagaatat 
gtgccaagg gatgtcacc gggagcttt ttggttaaa 
gattcagcg ggagtgatt tattcagga aggagggtt 
ttgttctcg attatttcg acattactt ctgttgcgt 
ttaatcgtc tag
    mRNA sequence
    • >TC011211 RA
      accatatga ttttacttt gaggactaa attataaaa 
gatgatgtt ttcaacttg agaacagca tttcgtttt 
cagtaacaa gttgagact ggctaagag gataaatga 
aacattcaa aattttcaa tgttttttt acttttttt 
tgtctaaaa ttgggcatt ttgtaagcc aacatcttc 
aaaatcagt taaaaataa ccactttgt agcatattt 
ctgtatttt tagccacct gacacatgg acgtcctat 
tatcagttc tcgaattat taatgactc ctcgcccat 
aataagtct gataactat gagccacat ccattattc 
cctcgttaa aaaagcaac tatctagca aaaataaat 
tttaatttg tacatttta cgccagtta ttgcaaggc 
caaaacaac aaaacgacg caatgttga caaaacttc 
tcctcctcg ccattctct gccaaagct ttgctgaag 
tctacgaga acacttttg actgcgaag accatttca 
gcgaaagcc acgaaaatg gtaccatag agtaccata 
acttcgtca ccacgaacg tgaaattct acctttaca 
ctcctccac ccgacaatg acaccatcg agctgaatt 
cgaccaact tacacttgg tcaatttga cccaaccca 
cgaaaatca tcatccatg gtctccagt cgtacgaaa 
accaggtat gggtggaca aaatggtac agaactacc 
atacaaagg gcgattaca acataatcg cggtggatt 
ggtccgtta tctcctcgg acgaaactt tctacgttg 
tttccgccg tggatgacc tcgggcggt acgtggggg 
acttcatca tggaggtca cccaggaca actaccagt 
acctctctg acgtccact tgatcggct cctcactag 
gtgcgcaaa tcgcagggg ctgctggaa gcgcaattt 
ccaacaaaa ccaacgcca gagttgacc gaataaccg 
gcctggacc caactcagc cggaaaact tgcagcttt 
tggacgaga acactgcag agtttgtcg atgttatcc 
atacgacgc tctactatg gccaggaga gttgtggga 
cggttgatt tttatgtca tggctgagg atccacaaa 
gacccattt gttataacg atacttgtg tagagcttt 
taggggatg actattacg cggtcacta tcatcgatg 
aggatagat ttgaagggg ttacatgct ataatacct 
ctctagccg ttcagagat tgtgtgata aggccaaat 
cggctgtta tgggagaat atgtgccaa gggatgtca 
ccgggagct ttttggtta aagattcag cgggagtga 
tttattcag gaaggaggg ttttgttct cgattattt 
cgacattac ttctgttgc gtttaatcg tctagtttt 
aaaatattt gtacttgtt ttagtttgt ctaggtaag 
taaattcag tttagaatt gtttgactt ttatcgagc 
ttaaaaata atatgaata ttaaatacg ctgtttttg 
ccgagttat caactggaa ttttaagtt gctttaatg 
tttgtatta ttataacaa ggttaaata cgtaagaaa 
taaagcgtt gattaaatg aatggcttt tacattcat 
tcaaaatac tgtaattaa aatcccact agaagttga 
atggtaatg cgtttcagc gatttttag tcatcctta 
tcgcttaat tatttaaaa aaatatggt ttaattaac 
tttgcctaa cgttattaa ttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY