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Gene information
General information
TC011211 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 2112664..2114511
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011211
    iBeetle screen iB_01827

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011211 RA
      MLTKLLLLA ILCQSFAEV YENTFDCED HFSESHENG 
      TIEYHNFVT TNVKFYLYT PPPDNDTIE LNSTNLHLV 
      NLTQPTKII IHGLQSYEN QVWVDKMVQ NYHTKGDYN 
      IIAVDWSVI SSDETFYVV SAVDDLGRY VGDFIMEVT 
      QDNYQYLSD VHLIGSSLG AQIAGAAGS AISNKTNAR 
      VDRITGLDP TQPENLQLL DENTAEFVD VIHTTLYYG 
      QESCGTVDF YVMAEDPQR PICYNDTCV ELLGDDYYA 
      VTIIDEDRF EGVTCYNTS LAVQRLCDK AKSAVMGEY 
      VPRDVTGSF LVKDSAGVI YSGRRVLFS IISTLLLLR 
      LIV
    CDS sequence
    • >TC011211 RA
      atgttgaca aaacttctc ctcctcgcc attctctgc 
      caaagcttt gctgaagtc tacgagaac acttttgac 
      tgcgaagac catttcagc gaaagccac gaaaatggt 
      accatagag taccataac ttcgtcacc acgaacgtg 
      aaattctac ctttacact cctccaccc gacaatgac 
      accatcgag ctgaattcg accaactta cacttggtc 
      aatttgacc caacccacg aaaatcatc atccatggt 
      ctccagtcg tacgaaaac caggtatgg gtggacaaa 
      atggtacag aactaccat acaaagggc gattacaac 
      ataatcgcg gtggattgg tccgttatc tcctcggac 
      gaaactttc tacgttgtt tccgccgtg gatgacctc 
      gggcggtac gtgggggac ttcatcatg gaggtcacc 
      caggacaac taccagtac ctctctgac gtccacttg 
      atcggctcc tcactaggt gcgcaaatc gcaggggct 
      gctggaagc gcaatttcc aacaaaacc aacgccaga 
      gttgaccga ataaccggc ctggaccca actcagccg 
      gaaaacttg cagcttttg gacgagaac actgcagag 
      tttgtcgat gttatccat acgacgctc tactatggc 
      caggagagt tgtgggacg gttgatttt tatgtcatg 
      gctgaggat ccacaaaga cccatttgt tataacgat 
      acttgtgta gagctttta ggggatgac tattacgcg 
      gtcactatc atcgatgag gatagattt gaaggggtt 
      acatgctat aatacctct ctagccgtt cagagattg 
      tgtgataag gccaaatcg gctgttatg ggagaatat 
      gtgccaagg gatgtcacc gggagcttt ttggttaaa 
      gattcagcg ggagtgatt tattcagga aggagggtt 
      ttgttctcg attatttcg acattactt ctgttgcgt 
      ttaatcgtc tag
    mRNA sequence
    • >TC011211 RA
      accatatga ttttacttt gaggactaa attataaaa 
      gatgatgtt ttcaacttg agaacagca tttcgtttt 
      cagtaacaa gttgagact ggctaagag gataaatga 
      aacattcaa aattttcaa tgttttttt acttttttt 
      tgtctaaaa ttgggcatt ttgtaagcc aacatcttc 
      aaaatcagt taaaaataa ccactttgt agcatattt 
      ctgtatttt tagccacct gacacatgg acgtcctat 
      tatcagttc tcgaattat taatgactc ctcgcccat 
      aataagtct gataactat gagccacat ccattattc 
      cctcgttaa aaaagcaac tatctagca aaaataaat 
      tttaatttg tacatttta cgccagtta ttgcaaggc 
      caaaacaac aaaacgacg caatgttga caaaacttc 
      tcctcctcg ccattctct gccaaagct ttgctgaag 
      tctacgaga acacttttg actgcgaag accatttca 
      gcgaaagcc acgaaaatg gtaccatag agtaccata 
      acttcgtca ccacgaacg tgaaattct acctttaca 
      ctcctccac ccgacaatg acaccatcg agctgaatt 
      cgaccaact tacacttgg tcaatttga cccaaccca 
      cgaaaatca tcatccatg gtctccagt cgtacgaaa 
      accaggtat gggtggaca aaatggtac agaactacc 
      atacaaagg gcgattaca acataatcg cggtggatt 
      ggtccgtta tctcctcgg acgaaactt tctacgttg 
      tttccgccg tggatgacc tcgggcggt acgtggggg 
      acttcatca tggaggtca cccaggaca actaccagt 
      acctctctg acgtccact tgatcggct cctcactag 
      gtgcgcaaa tcgcagggg ctgctggaa gcgcaattt 
      ccaacaaaa ccaacgcca gagttgacc gaataaccg 
      gcctggacc caactcagc cggaaaact tgcagcttt 
      tggacgaga acactgcag agtttgtcg atgttatcc 
      atacgacgc tctactatg gccaggaga gttgtggga 
      cggttgatt tttatgtca tggctgagg atccacaaa 
      gacccattt gttataacg atacttgtg tagagcttt 
      taggggatg actattacg cggtcacta tcatcgatg 
      aggatagat ttgaagggg ttacatgct ataatacct 
      ctctagccg ttcagagat tgtgtgata aggccaaat 
      cggctgtta tgggagaat atgtgccaa gggatgtca 
      ccgggagct ttttggtta aagattcag cgggagtga 
      tttattcag gaaggaggg ttttgttct cgattattt 
      cgacattac ttctgttgc gtttaatcg tctagtttt 
      aaaatattt gtacttgtt ttagtttgt ctaggtaag 
      taaattcag tttagaatt gtttgactt ttatcgagc 
      ttaaaaata atatgaata ttaaatacg ctgtttttg 
      ccgagttat caactggaa ttttaagtt gctttaatg 
      tttgtatta ttataacaa ggttaaata cgtaagaaa 
      taaagcgtt gattaaatg aatggcttt tacattcat 
      tcaaaatac tgtaattaa aatcccact agaagttga 
      atggtaatg cgtttcagc gatttttag tcatcctta 
      tcgcttaat tatttaaaa aaatatggt ttaattaac 
      tttgcctaa cgttattaa ttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY