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Gene information
General information
TC011205 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 2319167..2332060
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011205
    iBeetle screen iB_01825

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized -
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011205 RA
      MDEYDEDEP NFEEQEFHN NVREQIIFL LLFLLLYLL 
      SFALLGRFK RKGREDYYS TDEDEVTVY RISTWLCTF 
      SLAVSIGAV LLLPISIIS NEVLIIYPN SYYIKWLNS 
      SLIQGLWNY IFLFSNLSL FVLLPFAYL FTESEGFFG 
      HRKGLMARV YETFTVLGL LGLVVLGMT YVISALIDK 
      DKSSFQTLL NLWSYYLPF LYSCISFLG VLMLLVCTP 
      LGFVRLFDV VSQFLIKPQ FLRDINEEY YACALEEEC 
      LRRRLKHVQ TTGKYYVSP APMSIGLNG PFHDDDYKL 
      PDNLLRLRN GELQSGLGS RLVEVETRR KMLDKQRHT 
      SFLRRNVLH PIAMILLIG LTVIAVLLV VQNTLSLLI 
      GIKALPLSS KQFTLGVSS LSKLGPFGA ALEIVLILY 
      LIATSSIGL YTAPVMSRI RPRPRSTPF CLIIGNCAV 
      VLILSSALP LLSKILGIT NFDLLGDFG SIEWLGNFQ 
      IVLLYNLSF AAASTLCVV NKFTAKVRR ELYARISLF 
      LGVCEPPVS VPNTPRPSV VKFA
    • >TC011205 RB
      MDEYDEDEP NFEEQEFHN NVREQIIFL LLFLLLYLL 
      SFALLGRFK RKGREDYYS TDEDEVTVY RISTWLCTF 
      SLAVSIGAV LLLPISIIS NEVLIIYPN SYYIKWLNS 
      SLIQGLWNY IFLFSNLSL FVLLPFAYL FTESEGFFG 
      HRKGLMARV YETFTVLGL LGLVVLGMT YVISALIDK 
      DKSSFQTLL NLWSYYLPF LYSCISFLG VLMLLVCTP 
      LGFVRLFDV VSQFLIKPQ FLRDINEEY YACALEEEC 
      LRRRLKHVQ TTGKYYVSP APMSIGLNG PFHDDDYKL 
      PDNLLRLRN GELQSGLGS RLVEVETRR KMLDKQRHT 
      SFLRRNVLH PIAMILLIG LTVIAVLLV VQNTLSLLI 
      GIKALPLSS KQFTLGVSS LSKLGPFGA ALEIVLILY 
      LIATSSIGL YTAPVMSRI RPRPRSTPF CLIIGNCAV 
      VLILSSALP LLSKILGIT NFDLLGDFG SIEWLGNFQ 
      IVLLYNLSF AAASTLCVV NKFTAKVRR ELYARLVEN 
      YMYLINCVT FLN
    CDS sequence
    • >TC011205 RA
      atggatgaa tatgacgaa gatgagccg aattttgaa 
      gaacaagaa ttccacaac aacgttcgg gaacaaatc 
      atctttttg ttgctgttc cttctcctc tacctctta 
      agtttcgcc cttttggga cgtttcaaa cgaaaaggc 
      cgtgaggat tactacagt accgacgag gacgaggtc 
      acagtctat cgtataagc acctggttg tgcacattc 
      tcattggct gtgtcaatc ggtgccgtt cttctttta 
      ccaatttcg ataatttcg aacgaagtg ctgattatc 
      taccctaat agctattac atcaaatgg ttgaacagt 
      tctcttatt cagggatta tggaattat attttttta 
      ttttccaat ttatcactt ttcgttctt ttgccattt 
      gcttattta ttcactgaa tcggaagga tttttcgga 
      caccgcaaa ggattgatg gccagagtc tacgaaact 
      tttaccgtt ttaggcctt ttaggactg gtggtgttg 
      ggtatgacg tatgtcata tcggcgctt atcgataaa 
      gataagtcc agttttcag actttactc aatttatgg 
      agctactac ttacctttt ttatactcg tgcatatca 
      ttcctgggc gttttgatg cttttggta tgtacccct 
      ttgggtttt gtgcgacta ttcgacgtc gtcagtcag 
      ttcctaata aaacctcag tttttacgc gacataaat 
      gaggaatac tacgcttgt gctttagaa gaagagtgc 
      ttgaggcga agactgaaa catgtacaa acaacgggc 
      aaatattac gtcagtccg gcccctatg tcgataggt 
      ctaaacggc ccttttcac gacgatgat tacaaacta 
      cctgataat ttattacgt ttacgaaac ggggaatta 
      cagtcgggg cttggctcg cgcctcgtt gaagtggaa 
      acacggaga aaaatgcta gataaacaa agacataca 
      tcgtttctg cgccgaaac gtcttgcat ccgattgcg 
      atgatactt ttgattggt ttgacagta attgcagta 
      ctgttggtt gtacaaaat acgctttct ttgcttatc 
      ggtattaaa gcattgccg cttagttca aagcaattt 
      acactagga gttagctcc ttgtccaaa ctaggtccc 
      tttggggct gctttggag attgtatta attttatac 
      ttaatcgca acttcctca ataggtctt tatacagct 
      cctgtgatg tctcggata cgtccgaga ccaagaagt 
      acaccgttt tgcttgatt ataggcaat tgtgccgtt 
      gtattgatt ctcagctcg gctttgcct ctgctgtcg 
      aagattttg ggtatcaca aattttgat ttattgggc 
      gatttcggc agcatcgag tggcttgga aacttccaa 
      atcgtgcta ctctacaac ttgtctttt gcagcagcg 
      tccactcta tgcgtagtc aataaattc acagcgaaa 
      gtgcgaaga gaactgtac gcccgaata tcgcttttt 
      ctgggagtt tgtgaacca cctgtatcc gtcccaaat 
      acaccaagg ccttcagtg gtcaagttt gcatag
    • >TC011205 RB
      atggatgaa tatgacgaa gatgagccg aattttgaa 
      gaacaagaa ttccacaac aacgttcgg gaacaaatc 
      atctttttg ttgctgttc cttctcctc tacctctta 
      agtttcgcc cttttggga cgtttcaaa cgaaaaggc 
      cgtgaggat tactacagt accgacgag gacgaggtc 
      acagtctat cgtataagc acctggttg tgcacattc 
      tcattggct gtgtcaatc ggtgccgtt cttctttta 
      ccaatttcg ataatttcg aacgaagtg ctgattatc 
      taccctaat agctattac atcaaatgg ttgaacagt 
      tctcttatt cagggatta tggaattat attttttta 
      ttttccaat ttatcactt ttcgttctt ttgccattt 
      gcttattta ttcactgaa tcggaagga tttttcgga 
      caccgcaaa ggattgatg gccagagtc tacgaaact 
      tttaccgtt ttaggcctt ttaggactg gtggtgttg 
      ggtatgacg tatgtcata tcggcgctt atcgataaa 
      gataagtcc agttttcag actttactc aatttatgg 
      agctactac ttacctttt ttatactcg tgcatatca 
      ttcctgggc gttttgatg cttttggta tgtacccct 
      ttgggtttt gtgcgacta ttcgacgtc gtcagtcag 
      ttcctaata aaacctcag tttttacgc gacataaat 
      gaggaatac tacgcttgt gctttagaa gaagagtgc 
      ttgaggcga agactgaaa catgtacaa acaacgggc 
      aaatattac gtcagtccg gcccctatg tcgataggt 
      ctaaacggc ccttttcac gacgatgat tacaaacta 
      cctgataat ttattacgt ttacgaaac ggggaatta 
      cagtcgggg cttggctcg cgcctcgtt gaagtggaa 
      acacggaga aaaatgcta gataaacaa agacataca 
      tcgtttctg cgccgaaac gtcttgcat ccgattgcg 
      atgatactt ttgattggt ttgacagta attgcagta 
      ctgttggtt gtacaaaat acgctttct ttgcttatc 
      ggtattaaa gcattgccg cttagttca aagcaattt 
      acactagga gttagctcc ttgtccaaa ctaggtccc 
      tttggggct gctttggag attgtatta attttatac 
      ttaatcgca acttcctca ataggtctt tatacagct 
      cctgtgatg tctcggata cgtccgaga ccaagaagt 
      acaccgttt tgcttgatt ataggcaat tgtgccgtt 
      gtattgatt ctcagctcg gctttgcct ctgctgtcg 
      aagattttg ggtatcaca aattttgat ttattgggc 
      gatttcggc agcatcgag tggcttgga aacttccaa 
      atcgtgcta ctctacaac ttgtctttt gcagcagcg 
      tccactcta tgcgtagtc aataaattc acagcgaaa 
      gtgcgaaga gaactgtac gcccggtta gttgaaaac 
      tatatgtat ttgattaac tgtgttaca tttctcaac 
      taa
    mRNA sequence
    • >TC011205 RA
      tatcaaaga tcggcgatt tcgagctta gggcttcct 
      cttgtgaat ttgatttag attaggttt ttccctggt 
      gtcgccagc gtcgccaca acacaattt gatttatgt 
      tgtcgtcct gcattccag ctggacatt gttctagta 
      aacgtcaag cgtcaaggt taataattt ttcgagttt 
      taaacgcaa aattaatcg tttgtataa aatccagtg 
      acagaagtt gtgaccagt ttttcgaac acttttacc 
      ctacgtttt attgcaaaa aagtcgaat aatggaccc 
      tcaaagtgt ttaaataat tttccacca tgaatagtt 
      taatagtgc tagaaggac cagtgttaa caaataggt 
      gaaagaaat ggatgaata tgacgaaga tgagccgaa 
      ttttgaaga acaagaatt ccacaacaa cgttcggga 
      acaaatcat ctttttgtt gctgttcct tctcctcta 
      cctcttaag tttcgccct tttgggacg tttcaaacg 
      aaaaggccg tgaggatta ctacagtac cgacgagga 
      cgaggtcac agtctatcg tataagcac ctggttgtg 
      cacattctc attggctgt gtcaatcgg tgccgttct 
      tcttttacc aatttcgat aatttcgaa cgaagtgct 
      gattatcta ccctaatag ctattacat caaatggtt 
      gaacagttc tcttattca gggattatg gaattatat 
      ttttttatt ttccaattt atcactttt cgttctttt 
      gccatttgc ttatttatt cactgaatc ggaaggatt 
      tttcggaca ccgcaaagg attgatggc cagagtcta 
      cgaaacttt taccgtttt aggcctttt aggactggt 
      ggtgttggg tatgacgta tgtcatatc ggcgcttat 
      cgataaaga taagtccag ttttcagac tttactcaa 
      tttatggag ctactactt acctttttt atactcgtg 
      catatcatt cctgggcgt tttgatgct tttggtatg 
      taccccttt gggttttgt gcgactatt cgacgtcgt 
      cagtcagtt cctaataaa acctcagtt tttacgcga 
      cataaatga ggaatacta cgcttgtgc tttagaaga 
      agagtgctt gaggcgaag actgaaaca tgtacaaac 
      aacgggcaa atattacgt cagtccggc ccctatgtc 
      gataggtct aaacggccc ttttcacga cgatgatta 
      caaactacc tgataattt attacgttt acgaaacgg 
      ggaattaca gtcggggct tggctcgcg cctcgttga 
      agtggaaac acggagaaa aatgctaga taaacaaag 
      acatacatc gtttctgcg ccgaaacgt cttgcatcc 
      gattgcgat gatactttt gattggttt gacagtaat 
      tgcagtact gttggttgt acaaaatac gctttcttt 
      gcttatcgg tattaaagc attgccgct tagttcaaa 
      gcaatttac actaggagt tagctcctt gtccaaact 
      aggtccctt tggggctgc tttggagat tgtattaat 
      tttatactt aatcgcaac ttcctcaat aggtcttta 
      tacagctcc tgtgatgtc tcggatacg tccgagacc 
      aagaagtac accgttttg cttgattat aggcaattg 
      tgccgttgt attgattct cagctcggc tttgcctct 
      gctgtcgaa gattttggg tatcacaaa ttttgattt 
      attgggcga tttcggcag catcgagtg gcttggaaa 
      cttccaaat cgtgctact ctacaactt gtcttttgc 
      agcagcgtc cactctatg cgtagtcaa taaattcac 
      agcgaaagt gcgaagaga actgtacgc ccgaatatc 
      gctttttct gggagtttg tgaaccacc tgtatccgt 
      cccaaatac accaaggcc ttcagtggt caagtttgc 
      atagagaat tatcaactt gttctgtga ttagtagaa 
      tagaatttc tcgaactga aaggctgag ttgagtaca 
      gtgcgtgac ttgaggcgg gggtttact gcacaaagt 
      cagatgctt ttctagtcg taagtttat tagttgttt 
      cacttgtta tgtattcat tgccttatt tatgtacta 
      cttctaggg attttatca aatatatta atttatatt 
      ta
    • >TC011205 RB
      tatcaaaga tcggcgatt tcgagctta gggcttcct 
      cttgtgaat ttgatttag attaggttt ttccctggt 
      gtcgccagc gtcgccaca acacaattt gatttatgt 
      tgtcgtcct gcattccag ctggacatt gttctagta 
      aacgtcaag cgtcaaggt taataattt ttcgagttt 
      taaacgcaa aattaatcg tttgtataa aatccagtg 
      acagaagtt gtgaccagt ttttcgaac acttttacc 
      ctacgtttt attgcaaaa aagtcgaat aatggaccc 
      tcaaagtgt ttaaataat tttccacca tgaatagtt 
      taatagtgc tagaaggac cagtgttaa caaataggt 
      gaaagaaat ggatgaata tgacgaaga tgagccgaa 
      ttttgaaga acaagaatt ccacaacaa cgttcggga 
      acaaatcat ctttttgtt gctgttcct tctcctcta 
      cctcttaag tttcgccct tttgggacg tttcaaacg 
      aaaaggccg tgaggatta ctacagtac cgacgagga 
      cgaggtcac agtctatcg tataagcac ctggttgtg 
      cacattctc attggctgt gtcaatcgg tgccgttct 
      tcttttacc aatttcgat aatttcgaa cgaagtgct 
      gattatcta ccctaatag ctattacat caaatggtt 
      gaacagttc tcttattca gggattatg gaattatat 
      ttttttatt ttccaattt atcactttt cgttctttt 
      gccatttgc ttatttatt cactgaatc ggaaggatt 
      tttcggaca ccgcaaagg attgatggc cagagtcta 
      cgaaacttt taccgtttt aggcctttt aggactggt 
      ggtgttggg tatgacgta tgtcatatc ggcgcttat 
      cgataaaga taagtccag ttttcagac tttactcaa 
      tttatggag ctactactt acctttttt atactcgtg 
      catatcatt cctgggcgt tttgatgct tttggtatg 
      taccccttt gggttttgt gcgactatt cgacgtcgt 
      cagtcagtt cctaataaa acctcagtt tttacgcga 
      cataaatga ggaatacta cgcttgtgc tttagaaga 
      agagtgctt gaggcgaag actgaaaca tgtacaaac 
      aacgggcaa atattacgt cagtccggc ccctatgtc 
      gataggtct aaacggccc ttttcacga cgatgatta 
      caaactacc tgataattt attacgttt acgaaacgg 
      ggaattaca gtcggggct tggctcgcg cctcgttga 
      agtggaaac acggagaaa aatgctaga taaacaaag 
      acatacatc gtttctgcg ccgaaacgt cttgcatcc 
      gattgcgat gatactttt gattggttt gacagtaat 
      tgcagtact gttggttgt acaaaatac gctttcttt 
      gcttatcgg tattaaagc attgccgct tagttcaaa 
      gcaatttac actaggagt tagctcctt gtccaaact 
      aggtccctt tggggctgc tttggagat tgtattaat 
      tttatactt aatcgcaac ttcctcaat aggtcttta 
      tacagctcc tgtgatgtc tcggatacg tccgagacc 
      aagaagtac accgttttg cttgattat aggcaattg 
      tgccgttgt attgattct cagctcggc tttgcctct 
      gctgtcgaa gattttggg tatcacaaa ttttgattt 
      attgggcga tttcggcag catcgagtg gcttggaaa 
      cttccaaat cgtgctact ctacaactt gtcttttgc 
      agcagcgtc cactctatg cgtagtcaa taaattcac 
      agcgaaagt gcgaagaga actgtacgc ccggttagt 
      tgaaaacta tatgtattt gattaactg tgttacatt 
      tctcaacta acccgagtt tagtataag actgatttt 
      atatatttg ttttttttt aattccaaa ctaaaacgc 
      ttatcactc aaaccattc attatcatc ttattaatc 
      atcctggtg tgaggtggc gatcgatga cttaccggc 
      attgtttat aaacagaat atcgctttt tctgggagt 
      ttgtgaacc acctgtatc cgtcccaaa tacaccaag 
      gccttcagt ggtcaagtt tgcatagag aattatcaa 
      cttgttctg tgattagta gaatagaat ttctcgaac 
      tgaaaggct gagttgagt acagtgcgt gacttgagg 
      cgggggttt actgcacaa agtcagatg cttttctag 
      tcgtaagtt tattagttg tttcacttg ttatgtatt 
      cattgcctt atttatgta ctacttcta gggatttta 
      tcaaatata ttaatttat attta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY