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Gene information
General information
TC011200 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid21: 3433992..3439676
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011200
    iBeetle screen iB_01823
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011200 RA
      MDVANVQVG QRQHQDEIG VRCQKLFQD FLEEFKEDG 
      EIKYLEPAK ELLNQERST IEVSFEDLE KYNQNLATT 
      VIEEYYRIY PYLCQAVSN FVKDRSDLK KDKECYISF 
      VDVPTRHKV RELKTEKVG TLIRISGQV IRTHPVHPE 
      LILGTFVCL DCQTVIKDV EQQFKFTNP TICRNPVCN 
      NRRRFMLDV DKSLFVDFQ KVRIQETQA ELPRGCIPR 
      CVEVILRAE NVETVQAGD RYDFTGTLI VVPDVGALA 
      LPGAKAQIN SRHKGGDDT EGVRGLKAL GVRDLHYRM 
      AFLACSVQP TNPKFGGIE MPLNEITPE IMRQQMTEG 
      EWNHMYEMS HDRNLYQNL INSLFPSIH GNEEVKRGI 
      LLMLFGGVS KTTVEGTTL RGDINCCIV GDPSTAKSQ 
      FLKQVSEFS PRSVYTSGK ASSAAGLTA AVVRDEESS 
      DFVIEAGAL MLADNGVCC IDEFDKMDP RDQIAIHEA 
      MEQQTISLA KAGVRATLN ARTSILAAA NPIGGRYDR 
      AKSLQQNIA LSAPIMSRF DLFFILVDE CNEVIDYAI 
      ARKIVDLHS NVEEALDRI YSKEEVLQY ISFVRRFKP 
      IISPEAAEL LVRHYNHLR LRDTTTSGK STWRITVRQ 
      LESMIRLAE AMAKMEISD EVQPKHVKE AYRLLNKSI 
      IRVEQPDIH LDEDANAPM VEDDSETMT QETEPQPIR 
      KKLQLSFED YKVLSNKIV VFMRREEAR RDEENEEGI 
      RRSDLVEWY LNLIAEDIE SEEELLEKK ELIEKVIDR 
      LIYHDQVII PLTRTGLGR DQQEEEDAM LVVHPNYII 
      D
    CDS sequence
    • >TC011200 RA
      atggatgta gccaacgtc caggtcggc cagaggcaa 
      caccaggat gaaatcggt gtcaggtgc cagaaatta 
      ttccaggat ttcttagaa gaatttaaa gaggacggc 
      gaaatcaag tatttggag ccggctaag gagctcctc 
      aaccaagag cgcagcact attgaagtc agttttgaa 
      gatttagag aaatataac caaaatctt gcaacgacc 
      gttatcgag gagtattac cggatttac ccttatctt 
      tgccaagcc gtctctaat ttcgttaaa gaccgcagc 
      gatttgaaa aaagataaa gagtgttat atcagcttt 
      gttgatgtt ccaacaaga cacaaagta cgagaattg 
      aaaactgag aaagttggg actttgatt cggatctca 
      ggacaggtt atcaggact caccctgta caccctgaa 
      ctgattttg gggactttc gtctgtttg gattgccaa 
      actgtcatt aaagatgtt gagcaacaa tttaagttt 
      actaatccg acgatttgt cgcaaccct gtttgcaat 
      aacaggaga cgctttatg ctagatgtg gacaagtcc 
      ctatttgtg gacttccag aaagtccgg atacaggaa 
      actcaagcc gagttgcca agggggtgc attccgagg 
      tgtgttgag gtgatttta agagccgaa aatgtggaa 
      acagtccag gccggcgat cgttatgac ttcacgggg 
      actctcatc gtggtcccg gacgtgggg gctttggct 
      cttcctggg gcgaaggct cagatcaac tcgcgtcac 
      aagggtggt gacgatact gaaggggtt aggggtctc 
      aaagcccta ggagtgcgt gatcttcat tacagaatg 
      gcgtttctc gcgtgtagt gttcagccc actaacccc 
      aaatttggg ggtattgag atgcctctt aatgagatt 
      actcccgaa attatgcga cagcagatg acagagggc 
      gagtggaat cacatgtat gagatgagc cacgacagg 
      aatttgtat cagaatctc atcaatagt ttatttccg 
      agtattcac gggaatgag gaggtcaaa agggggata 
      cttttgatg ctgtttggg ggagtttcc aagacgaca 
      gttgaaggg acaaccctt cggggcgac atcaactgc 
      tgcatagtg ggcgacccc agcactgcc aaatcgcaa 
      tttctgaaa caagtttcg gaattttcc cctcgttct 
      gtctacact tcgggcaaa gcctcctcg gctgcaggt 
      ctcacagcc gctgtggtg cgcgatgaa gaatcctcc 
      gattttgtg atcgaagct ggcgctcta atgcttgcc 
      gataatggc gtttgctgt attgacgaa ttcgacaaa 
      atggaccca cgtgaccag atcgcgatc cacgaagcc 
      atggaacag caaacgatc tcattggcc aaagccgga 
      gtgcgggcc acgcttaac gcccgcacc tccatccta 
      gctgcagcc aatcccatt ggagggcgc tacgatcgc 
      gccaagtcg ctccaacag aacatcgcg ctaagtgcg 
      ccaatcatg tcccgattt gatttgttt tttatccta 
      gttgatgag tgtaatgag gtgattgat tatgctatt 
      gcgcggaag attgtggat ttgcacagt aatgtggag 
      gaggccctc gaccggatt tactcaaaa gaggaggtt 
      ttgcagtat atttcgttc gtgcggagg tttaagcca 
      atcataagt cccgaggct gcggagttg cttgtgagg 
      cactacaac catttgagg ttgagggac accacaact 
      agtggtaaa tcaacttgg cggattaca gtacgccaa 
      cttgaaagt atgatccga ttggcggaa gccatggcc 
      aagatggag atttccgac gaagtccaa ccaaaacac 
      gtcaaggag gcgtaccgc ctacttaat aaatccatc 
      atcagggtg gaacaaccc gatatacac ctcgacgaa 
      gacgcaaat gcgccaatg gtagaggac gactcggaa 
      accatgact caggaaact gagccgcaa ccaatcagg 
      aagaagttg cagctgagt ttcgaggat tacaaagtt 
      ttgagtaac aagatcgtg gtgtttatg cgacgggag 
      gaggcgagg agggatgag gagaacgag gaggggatc 
      aggaggagt gatttggtg gagtggtac ttgaatctg 
      attgcggag gatattgag tccgaggag gagttgttg 
      gagaagaag gagttgatt gagaaagtt atagaccgg 
      ttgatttat cacgatcaa gttattatt cccttgacg 
      aggacgggg cttgggagg gatcagcag gaggaggag 
      gatgccatg ttggtggtt catcccaat tatattatc 
      gattga
    mRNA sequence
    • >TC011200 RA
      aaaatgtat ggttatgtt cgtagtcac tttcccgcc 
      ttttttgcg ttattgttt actctgttt ttcccgaat 
      ttttcgttc taaatcgct aaaacatgg atgtagcca 
      acgtccagg tcggccaga ggcaacacc aggatgaaa 
      tcggtgtca ggtgccaga aattattcc aggatttct 
      tagaagaat ttaaagagg acggcgaaa tcaagtatt 
      tggagccgg ctaaggagc tcctcaacc aagagcgca 
      gcactattg aagtcagtt ttgaagatt tagagaaat 
      ataaccaaa atcttgcaa cgaccgtta tcgaggagt 
      attaccgga tttaccctt atctttgcc aagccgtct 
      ctaatttcg ttaaagacc gcagcgatt tgaaaaaag 
      ataaagagt gttatatca gctttgttg atgttccaa 
      caagacaca aagtacgag aattgaaaa ctgagaaag 
      ttgggactt tgattcgga tctcaggac aggttatca 
      ggactcacc ctgtacacc ctgaactga ttttgggga 
      ctttcgtct gtttggatt gccaaactg tcattaaag 
      atgttgagc aacaattta agtttacta atccgacga 
      tttgtcgca accctgttt gcaataaca ggagacgct 
      ttatgctag atgtggaca agtccctat ttgtggact 
      tccagaaag tccggatac aggaaactc aagccgagt 
      tgccaaggg ggtgcattc cgaggtgtg ttgaggtga 
      ttttaagag ccgaaaatg tggaaacag tccaggccg 
      gcgatcgtt atgacttca cggggactc tcatcgtgg 
      tcccggacg tgggggctt tggctcttc ctggggcga 
      aggctcaga tcaactcgc gtcacaagg gtggtgacg 
      atactgaag gggttaggg gtctcaaag ccctaggag 
      tgcgtgatc ttcattaca gaatggcgt ttctcgcgt 
      gtagtgttc agcccacta accccaaat ttgggggta 
      ttgagatgc ctcttaatg agattactc ccgaaatta 
      tgcgacagc agatgacag agggcgagt ggaatcaca 
      tgtatgaga tgagccacg acaggaatt tgtatcaga 
      atctcatca atagtttat ttccgagta ttcacggga 
      atgaggagg tcaaaaggg ggatacttt tgatgctgt 
      ttgggggag tttccaaga cgacagttg aagggacaa 
      cccttcggg gcgacatca actgctgca tagtgggcg 
      accccagca ctgccaaat cgcaatttc tgaaacaag 
      tttcggaat tttcccctc gttctgtct acacttcgg 
      gcaaagcct cctcggctg caggtctca cagccgctg 
      tggtgcgcg atgaagaat cctccgatt ttgtgatcg 
      aagctggcg ctctaatgc ttgccgata atggcgttt 
      gctgtattg acgaattcg acaaaatgg acccacgtg 
      accagatcg cgatccacg aagccatgg aacagcaaa 
      cgatctcat tggccaaag ccggagtgc gggccacgc 
      ttaacgccc gcacctcca tcctagctg cagccaatc 
      ccattggag ggcgctacg atcgcgcca agtcgctcc 
      aacagaaca tcgcgctaa gtgcgccaa tcatgtccc 
      gatttgatt tgtttttta tcctagttg atgagtgta 
      atgaggtga ttgattatg ctattgcgc ggaagattg 
      tggatttgc acagtaatg tggaggagg ccctcgacc 
      ggatttact caaaagagg aggttttgc agtatattt 
      cgttcgtgc ggaggttta agccaatca taagtcccg 
      aggctgcgg agttgcttg tgaggcact acaaccatt 
      tgaggttga gggacacca caactagtg gtaaatcaa 
      cttggcgga ttacagtac gccaacttg aaagtatga 
      tccgattgg cggaagcca tggccaaga tggagattt 
      ccgacgaag tccaaccaa aacacgtca aggaggcgt 
      accgcctac ttaataaat ccatcatca gggtggaac 
      aacccgata tacacctcg acgaagacg caaatgcgc 
      caatggtag aggacgact cggaaacca tgactcagg 
      aaactgagc cgcaaccaa tcaggaaga agttgcagc 
      tgagtttcg aggattaca aagttttga gtaacaaga 
      tcgtggtgt ttatgcgac gggaggagg cgaggaggg 
      atgaggaga acgaggagg ggatcagga ggagtgatt 
      tggtggagt ggtacttga atctgattg cggaggata 
      ttgagtccg aggaggagt tgttggaga agaaggagt 
      tgattgaga aagttatag accggttga tttatcacg 
      atcaagtta ttattccct tgacgagga cggggcttg 
      ggagggatc agcaggagg aggaggatg ccatgttgg 
      tggttcatc ccaattata ttatcgatt gagtgtacc 
      tattttttt taataaaag ttaagtact aat