General information | |||
TC034615 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 19517246..19541658 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC034615 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Quotation | ||
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Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01822 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
adult lethality - Number of animals showing the phenotype: 6 (19 dpi)
adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
- >TC034615 RA
MQCVAAFIS LLCVLRAAN CDNRTKRFT VEDLLSTTF PKKVSDDQD LDPCKAGGF MGDIALPIK PKPKDSLLQ EEVEKYKQE VLEGLQIEE EGLTDFIRK KTKQPAPIP TPRSYNDPF DDISGVANP THKNGQILK PAAGFPNPK NDTKIRHNK RHAEVRRRR KRNRKGDSD ETPVHDFEP NFDMKRKRP LLNENSHHR RVTRAATAR KERVWDYGV IPYEIDGNF SGGHKALFK QAMRHWENF TCVKFVERN RDEHPNYIV FTERPCGCC SFVGKRGNG GQAISIGKN CDKFGIVVH ELGHVVGFW HEHTRPDRD KHVNIIREN IMSGQEYNF NKLNDEEVN SLGLIYDYD SIMHYARNT FSKGTYLDT IQPIDVPNR KRPEIGQRV RLSEGDIAQ TNLLYKCIK CGRTYQANS ATFSSPSYL NHNAPEDGE KCEWRITAT HGEKIVLNI TDLDIEKSP DCGTDYIEI RDGYWHKSP LLGKFCGTG KLDSITSTG SRMLINYVA KNPNGHRGF VANYEAICG GELYIDSEG HLESPNYPE EYQPNKECL WKITVPENY QVALRFQSF EVENHDGCV YDYVEIRDG FAPDSTILG VHCGYKVPS DVISSSNHL LVKFVSDGS VQKGGFSAT IMKEYDECA MIDHGCAQE CVNTLGSYE CTCKIGFEL HSDGKNCED ACGGLLDAP NGTITSPSF PDLYPLNKN CVWEIVSLP QYRITLNFT HFDLEGNNM HHAQQQQCD YDRLEIYSK LGDEKMKKH GVFCGSRAP LPIISEGNV MRIVFSSDV SVQKTGFAA VFFTDMDEC AINNGDCQH ECVNTVGSY KCTCHNGFT LHENGRDCK EGDCKYEIS LPFGSIGSP NYPDYYPSR KDCVWQFTT TPGHRIRIS FLFFEVEPH QECYYDHVD FYDGPSPES STLGKFCGS KLPHPIVSS GNELYMTFK SDASVQRKG FWATYAAVC GGILQATPK KKHIYSHAK FGTANYDNK ADCDWTIKA ISGYNVRLS FLTFDLEDE KDCGYDYVE VFSGMDSSG PSYGRFCGS TKPFDIFSP YDALLVRFR SDDTFVSKG FSIVYEAVE EAFSGEDEV
- >TC034615 RA
atgcagtgt gtggcggcc tttatcagt ttgttgtgt gtcttgcgt gccgcgaac tgtgataac aggactaag cggttcacc gtcgaggac ctcctctcc accaccttc ccgaagaag gtctccgac gaccaggac ctggacccc tgcaaggcc ggtgggttc atgggcgac atcgccttg cccatcaag cccaagccc aaggactcg ctgctccag gaggaagtg gagaagtac aagcaggag gtgctcgaa gggctccag attgaggaa gaagggctc acggacttc atccggaag aagaccaag cagccggcg cccatccca acgccccgg agttacaac gacccgttt gatgatatt tccggagtg gcgaatccg acgcacaaa aacggccaa attctcaaa cccgccgcc ggatttccg aatccgaaa aacgatacg aagattcgg cataataag agacatgcg gaggtcaga cggagacgg aaacggaat cggaaaggg gattcagac gagacgccg gttcatgat tttgagccg aattttgat atgaagagg aaacggcca cttttgaac gaaaattcc caccatagg agagtgaca agagcggca acggcacgg aaagaaaga gtctgggat tatggggtc attccctat gaaattgac gggaatttt agtggagga cataaggca ttgttcaag caggcgatg aggcattgg gagaatttc acatgtgtc aagtttgtg gaaaggaat agggatgag caccccaat tatattgtc ttcacggaa aggccttgt ggatgttgt tctttcgtc ggaaaaagg ggcaacgga ggccaagca atcagcatt ggaaaaaac tgcgacaaa ttcggaatt gtcgtacat gaactgggt cacgtggtt ggattttgg catgagcac actagacct gaccgggat aaacatgtt aatataatt cgggagaat attatgagt ggtcaagaa tacaatttt aataaatta aacgatgaa gaagtgaat tctctgggc ctaatttat gactacgat tcaatcatg cattatgca cgaaacaca ttctcaaag gggacttat ttagataca atccagccc atagatgtc cccaatagg aagcgtcct gaaatcgga caaagagtc cgtttgagc gaaggcgat atagctcaa acgaatctt ctttataag tgtattaaa tgtgggagg acctatcag gccaactcg gccaccttc tcctccccc tcctacctc aaccacaac gcccccgaa gacggcgaa aagtgcgaa tggcggatc acagcaacc catggcgag aaaatcgtc ttgaacatc accgacctg gacatcgaa aaatccccc gactgtgga accgactac atcgaaatc cgcgacggc tattggcac aaatctccc cttctggga aaattctgc ggcaccgga aaactcgac tccatcacg tcgaccgga agccgaatg ctgatcaac tacgtggcg aaaaacccg aacggccat cgcggtttc gtggccaac tacgaggcc atctgtggg ggcgagctg tacatcgac agtgagggc cacttggag tccccaaac taccccgag gagtaccaa cccaacaaa gagtgcctc tggaagatc acagttccg gagaactac caagtggct ctcagattt cagtcgttc gaagtcgag aaccacgat gggtgcgtt tacgactac gtggaaatc cgggacggg tttgcgccg gatagtacc attctgggc gtgcactgc gggtacaag gtcccatcg gatgtcatc tcgagctcc aaccatctc ctggtcaag ttcgtctcg gatgggtcg gtgcagaaa ggcggcttt tcggccacc atcatgaag gagtacgac gagtgtgcc atgatcgac catgggtgc gcccaggag tgcgtcaac acgctgggc agctacgag tgcacctgc aagatcggc ttcgagctg cactccgat gggaaaaac tgcgaggat gcttgtggc ggactcctg gacgcgccc aatgggact atcacgtct ccgtccttc ccagatttg tacccattg aacaagaac tgcgtttgg gagattgtg tccttgccg cagtaccgg attactcta aatttcacg cattttgac ctggagggg aataatatg caccacgcg cagcaacaa cagtgcgac tacgaccgg ctggaaatc tacagtaaa ttaggggat gaaaagatg aaaaaacac ggagttttt tgcgggtcg agggcccct cttcccatc atttcagag ggaaatgtg atgagaatt gtcttcagt tcggacgtt agtgtccaa aagactggc tttgcggct gttttcttc acagatatg gacgaatgt gccataaac aacggcgac tgccagcac gaatgcgtc aacactgtc ggttcctac aaatgcacc tgccataac ggcttcacc ctccacgaa aacgggcgc gattgcaaa gagggcgac tgcaagtac gaaatctcc ctccctttc ggaagcatc ggaagtcca aactacccc gactactac cccagtcgc aaagactgc gtctggcaa ttcaccaca acccccggc caccgaatc cgaatttcc ttcctattt ttcgaagtc gagccccat caagagtgc tactacgac cacgtggac ttctacgac ggcccttcg cccgagtcc agcactttg gggaagttc tgcggctcg aagttaccc catcctata gtttcctcc gggaatgaa ctctatatg acgttcaaa tctgacgcc tcagtccag aggaaaggg ttctgggcc acttatgcc gcagtctgt ggagggatt ctccaagcg acgcccaag aaaaagcac atttattcc catgcgaag tttgggacg gctaattat gacaataag gccgattgt gattggacg attaaggcc atttcgggg tataatgtc agactgtcg tttctgacg tttgatttg gaggatgag aaggactgt gggtatgat tatgtggag gtgttcagc gggatggac tcgagtggg ccctcctat ggccgattt tgcggctca acgaaacca ttcgatatt ttctcgccg tacgacgcc ctcttggtc cgttttcgc tccgacgac acttttgtg agcaaaggt tttagcata gtttacgaa gccgtcgaa gaggcgttc agtggcgaa gacgaagtg tag
- >TC034615 RA
tcgcgttct agttgcaag gcctcgagt aaacacgcg cttctcgat tgtcgtcgt cttcaggag ggaaatgca ggcacgagt cgcgcaagt gatctttgt ccgcttggc caaacatct ctgataatg ccataaacc cagcgcttc cattcgaaa aacgatgtg atttcaccc tagtccagt gttcgcgtc ggaagcatg cagtgtgtg gcggccttt atcagtttg ttgtgtgtc ttgcgtgcc gcgaactgt gataacagg actaagcgg ttcaccgtc gaggacctc ctctccacc accttcccg aagaaggtc tccgacgac caggacctg gacccctgc aaggccggt gggttcatg ggcgacatc gccttgccc atcaagccc aagcccaag gactcgctg ctccaggag gaagtggag aagtacaag caggaggtg ctcgaaggg ctccagatt gaggaagaa gggctcacg gacttcatc cggaagaag accaagcag ccggcgccc atcccaacg ccccggagt tacaacgac ccgtttgat gatatttcc ggagtggcg aatccgacg cacaaaaac ggccaaatt ctcaaaccc gccgccgga tttccgaat ccgaaaaac gatacgaag attcggcat aataagaga catgcggag gtcagacgg agacggaaa cggaatcgg aaaggggat tcagacgag acgccggtt catgatttt gagccgaat tttgatatg aagaggaaa cggccactt ttgaacgaa aattcccac cataggaga gtgacaaga gcggcaacg gcacggaaa gaaagagtc tgggattat ggggtcatt ccctatgaa attgacggg aattttagt ggaggacat aaggcattg ttcaagcag gcgatgagg cattgggag aatttcaca tgtgtcaag tttgtggaa aggaatagg gatgagcac cccaattat attgtcttc acggaaagg ccttgtgga tgttgttct ttcgtcgga aaaaggggc aacggaggc caagcaatc agcattgga aaaaactgc gacaaattc ggaattgtc gtacatgaa ctgggtcac gtggttgga ttttggcat gagcacact agacctgac cgggataaa catgttaat ataattcgg gagaatatt atgagtggt caagaatac aattttaat aaattaaac gatgaagaa gtgaattct ctgggccta atttatgac tacgattca atcatgcat tatgcacga aacacattc tcaaagggg acttattta gatacaatc cagcccata gatgtcccc aataggaag cgtcctgaa atcggacaa agagtccgt ttgagcgaa ggcgatata gctcaaacg aatcttctt tataagtgt attaaatgt gggaggacc tatcaggcc aactcggcc accttctcc tccccctcc tacctcaac cacaacgcc cccgaagac ggcgaaaag tgcgaatgg cggatcaca gcaacccat ggcgagaaa atcgtcttg aacatcacc gacctggac atcgaaaaa tcccccgac tgtggaacc gactacatc gaaatccgc gacggctat tggcacaaa tctcccctt ctgggaaaa ttctgcggc accggaaaa ctcgactcc atcacgtcg accggaagc cgaatgctg atcaactac gtggcgaaa aacccgaac ggccatcgc ggtttcgtg gccaactac gaggccatc tgtgggggc gagctgtac atcgacagt gagggccac ttggagtcc ccaaactac cccgaggag taccaaccc aacaaagag tgcctctgg aagatcaca gttccggag aactaccaa gtggctctc agatttcag tcgttcgaa gtcgagaac cacgatggg tgcgtttac gactacgtg gaaatccgg gacgggttt gcgccggat agtaccatt ctgggcgtg cactgcggg tacaaggtc ccatcggat gtcatctcg agctccaac catctcctg gtcaagttc gtctcggat gggtcggtg cagaaaggc ggcttttcg gccaccatc atgaaggag tacgacgag tgtgccatg atcgaccat gggtgcgcc caggagtgc gtcaacacg ctgggcagc tacgagtgc acctgcaag atcggcttc gagctgcac tccgatggg aaaaactgc gaggatgct tgtggcgga ctcctggac gcgcccaat gggactatc acgtctccg tccttccca gatttgtac ccattgaac aagaactgc gtttgggag attgtgtcc ttgccgcag taccggatt actctaaat ttcacgcat tttgacctg gaggggaat aatatgcac cacgcgcag caacaacag tgcgactac gaccggctg gaaatctac agtaaatta ggggatgaa aagatgaaa aaacacgga gttttttgc gggtcgagg gcccctctt cccatcatt tcagaggga aatgtgatg agaattgtc ttcagttcg gacgttagt gtccaaaag actggcttt gcggctgtt ttcttcaca gatatggac gaatgtgcc ataaacaac ggcgactgc cagcacgaa tgcgtcaac actgtcggt tcctacaaa tgcacctgc cataacggc ttcaccctc cacgaaaac gggcgcgat tgcaaagag ggcgactgc aagtacgaa atctccctc cctttcgga agcatcgga agtccaaac taccccgac tactacccc agtcgcaaa gactgcgtc tggcaattc accacaacc cccggccac cgaatccga atttccttc ctatttttc gaagtcgag ccccatcaa gagtgctac tacgaccac gtggacttc tacgacggc ccttcgccc gagtccagc actttgggg aagttctgc ggctcgaag ttaccccat cctatagtt tcctccggg aatgaactc tatatgacg ttcaaatct gacgcctca gtccagagg aaagggttc tgggccact tatgccgca gtctgtgga gggattctc caagcgacg cccaagaaa aagcacatt tattcccat gcgaagttt gggacggct aattatgac aataaggcc gattgtgat tggacgatt aaggccatt tcggggtat aatgtcaga ctgtcgttt ctgacgttt gatttggag gatgagaag gactgtggg tatgattat gtggaggtg ttcagcggg atggactcg agtgggccc tcctatggc cgattttgc ggctcaacg aaaccattc gatattttc tcgccgtac gacgccctc ttggtccgt tttcgctcc gacgacact tttgtgagc aaaggtttt agcatagtt tacgaagcc gtcgaagag gcgttcagt ggcgaagac gaagtgtag agtccagca gcaaactct tcgagttga ttgtcgttt ggtccagct tcgaataga gggcgcggt gttccaacg aagtgcgaa actcaataa actcagatt tgctcaata tatgaatta ctaggccgt ttctagtat ttatttcaa atattcgta cttatacta gtgaaatgt cttcgtaaa tacctgata ggaggataa tttgtcatt gttcgattt attattaat ggataaaca cttataata aacagactg ccgacttta gattttatg tcacgactc tatggatat tcatatacc gctctatag ctaggttaa cgataggat ttacaagtt gttatcaaa ttcgacgtg ccattttta agttaagga cgctggaat tgtacagat tttcgtgag ctcatgtat acatctctt gtgtaagtt atgaataaa tattatgtc gagta
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
