Status
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Gene information
General information
TC034615 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19517246..19541658
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034615
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01822

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Pupal morphology
    wings shortened - Number of animals showing the phenotype: 7

    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 9


    elytra shortened - Number of animals showing the phenotype: 7


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034615 RA
      MQCVAAFIS LLCVLRAAN CDNRTKRFT VEDLLSTTF 
      PKKVSDDQD LDPCKAGGF MGDIALPIK PKPKDSLLQ 
      EEVEKYKQE VLEGLQIEE EGLTDFIRK KTKQPAPIP 
      TPRSYNDPF DDISGVANP THKNGQILK PAAGFPNPK 
      NDTKIRHNK RHAEVRRRR KRNRKGDSD ETPVHDFEP 
      NFDMKRKRP LLNENSHHR RVTRAATAR KERVWDYGV 
      IPYEIDGNF SGGHKALFK QAMRHWENF TCVKFVERN 
      RDEHPNYIV FTERPCGCC SFVGKRGNG GQAISIGKN 
      CDKFGIVVH ELGHVVGFW HEHTRPDRD KHVNIIREN 
      IMSGQEYNF NKLNDEEVN SLGLIYDYD SIMHYARNT 
      FSKGTYLDT IQPIDVPNR KRPEIGQRV RLSEGDIAQ 
      TNLLYKCIK CGRTYQANS ATFSSPSYL NHNAPEDGE 
      KCEWRITAT HGEKIVLNI TDLDIEKSP DCGTDYIEI 
      RDGYWHKSP LLGKFCGTG KLDSITSTG SRMLINYVA 
      KNPNGHRGF VANYEAICG GELYIDSEG HLESPNYPE 
      EYQPNKECL WKITVPENY QVALRFQSF EVENHDGCV 
      YDYVEIRDG FAPDSTILG VHCGYKVPS DVISSSNHL 
      LVKFVSDGS VQKGGFSAT IMKEYDECA MIDHGCAQE 
      CVNTLGSYE CTCKIGFEL HSDGKNCED ACGGLLDAP 
      NGTITSPSF PDLYPLNKN CVWEIVSLP QYRITLNFT 
      HFDLEGNNM HHAQQQQCD YDRLEIYSK LGDEKMKKH 
      GVFCGSRAP LPIISEGNV MRIVFSSDV SVQKTGFAA 
      VFFTDMDEC AINNGDCQH ECVNTVGSY KCTCHNGFT 
      LHENGRDCK EGDCKYEIS LPFGSIGSP NYPDYYPSR 
      KDCVWQFTT TPGHRIRIS FLFFEVEPH QECYYDHVD 
      FYDGPSPES STLGKFCGS KLPHPIVSS GNELYMTFK 
      SDASVQRKG FWATYAAVC GGILQATPK KKHIYSHAK 
      FGTANYDNK ADCDWTIKA ISGYNVRLS FLTFDLEDE 
      KDCGYDYVE VFSGMDSSG PSYGRFCGS TKPFDIFSP 
      YDALLVRFR SDDTFVSKG FSIVYEAVE EAFSGEDEV
    CDS sequence
    • >TC034615 RA
      atgcagtgt gtggcggcc tttatcagt ttgttgtgt 
      gtcttgcgt gccgcgaac tgtgataac aggactaag 
      cggttcacc gtcgaggac ctcctctcc accaccttc 
      ccgaagaag gtctccgac gaccaggac ctggacccc 
      tgcaaggcc ggtgggttc atgggcgac atcgccttg 
      cccatcaag cccaagccc aaggactcg ctgctccag 
      gaggaagtg gagaagtac aagcaggag gtgctcgaa 
      gggctccag attgaggaa gaagggctc acggacttc 
      atccggaag aagaccaag cagccggcg cccatccca 
      acgccccgg agttacaac gacccgttt gatgatatt 
      tccggagtg gcgaatccg acgcacaaa aacggccaa 
      attctcaaa cccgccgcc ggatttccg aatccgaaa 
      aacgatacg aagattcgg cataataag agacatgcg 
      gaggtcaga cggagacgg aaacggaat cggaaaggg 
      gattcagac gagacgccg gttcatgat tttgagccg 
      aattttgat atgaagagg aaacggcca cttttgaac 
      gaaaattcc caccatagg agagtgaca agagcggca 
      acggcacgg aaagaaaga gtctgggat tatggggtc 
      attccctat gaaattgac gggaatttt agtggagga 
      cataaggca ttgttcaag caggcgatg aggcattgg 
      gagaatttc acatgtgtc aagtttgtg gaaaggaat 
      agggatgag caccccaat tatattgtc ttcacggaa 
      aggccttgt ggatgttgt tctttcgtc ggaaaaagg 
      ggcaacgga ggccaagca atcagcatt ggaaaaaac 
      tgcgacaaa ttcggaatt gtcgtacat gaactgggt 
      cacgtggtt ggattttgg catgagcac actagacct 
      gaccgggat aaacatgtt aatataatt cgggagaat 
      attatgagt ggtcaagaa tacaatttt aataaatta 
      aacgatgaa gaagtgaat tctctgggc ctaatttat 
      gactacgat tcaatcatg cattatgca cgaaacaca 
      ttctcaaag gggacttat ttagataca atccagccc 
      atagatgtc cccaatagg aagcgtcct gaaatcgga 
      caaagagtc cgtttgagc gaaggcgat atagctcaa 
      acgaatctt ctttataag tgtattaaa tgtgggagg 
      acctatcag gccaactcg gccaccttc tcctccccc 
      tcctacctc aaccacaac gcccccgaa gacggcgaa 
      aagtgcgaa tggcggatc acagcaacc catggcgag 
      aaaatcgtc ttgaacatc accgacctg gacatcgaa 
      aaatccccc gactgtgga accgactac atcgaaatc 
      cgcgacggc tattggcac aaatctccc cttctggga 
      aaattctgc ggcaccgga aaactcgac tccatcacg 
      tcgaccgga agccgaatg ctgatcaac tacgtggcg 
      aaaaacccg aacggccat cgcggtttc gtggccaac 
      tacgaggcc atctgtggg ggcgagctg tacatcgac 
      agtgagggc cacttggag tccccaaac taccccgag 
      gagtaccaa cccaacaaa gagtgcctc tggaagatc 
      acagttccg gagaactac caagtggct ctcagattt 
      cagtcgttc gaagtcgag aaccacgat gggtgcgtt 
      tacgactac gtggaaatc cgggacggg tttgcgccg 
      gatagtacc attctgggc gtgcactgc gggtacaag 
      gtcccatcg gatgtcatc tcgagctcc aaccatctc 
      ctggtcaag ttcgtctcg gatgggtcg gtgcagaaa 
      ggcggcttt tcggccacc atcatgaag gagtacgac 
      gagtgtgcc atgatcgac catgggtgc gcccaggag 
      tgcgtcaac acgctgggc agctacgag tgcacctgc 
      aagatcggc ttcgagctg cactccgat gggaaaaac 
      tgcgaggat gcttgtggc ggactcctg gacgcgccc 
      aatgggact atcacgtct ccgtccttc ccagatttg 
      tacccattg aacaagaac tgcgtttgg gagattgtg 
      tccttgccg cagtaccgg attactcta aatttcacg 
      cattttgac ctggagggg aataatatg caccacgcg 
      cagcaacaa cagtgcgac tacgaccgg ctggaaatc 
      tacagtaaa ttaggggat gaaaagatg aaaaaacac 
      ggagttttt tgcgggtcg agggcccct cttcccatc 
      atttcagag ggaaatgtg atgagaatt gtcttcagt 
      tcggacgtt agtgtccaa aagactggc tttgcggct 
      gttttcttc acagatatg gacgaatgt gccataaac 
      aacggcgac tgccagcac gaatgcgtc aacactgtc 
      ggttcctac aaatgcacc tgccataac ggcttcacc 
      ctccacgaa aacgggcgc gattgcaaa gagggcgac 
      tgcaagtac gaaatctcc ctccctttc ggaagcatc 
      ggaagtcca aactacccc gactactac cccagtcgc 
      aaagactgc gtctggcaa ttcaccaca acccccggc 
      caccgaatc cgaatttcc ttcctattt ttcgaagtc 
      gagccccat caagagtgc tactacgac cacgtggac 
      ttctacgac ggcccttcg cccgagtcc agcactttg 
      gggaagttc tgcggctcg aagttaccc catcctata 
      gtttcctcc gggaatgaa ctctatatg acgttcaaa 
      tctgacgcc tcagtccag aggaaaggg ttctgggcc 
      acttatgcc gcagtctgt ggagggatt ctccaagcg 
      acgcccaag aaaaagcac atttattcc catgcgaag 
      tttgggacg gctaattat gacaataag gccgattgt 
      gattggacg attaaggcc atttcgggg tataatgtc 
      agactgtcg tttctgacg tttgatttg gaggatgag 
      aaggactgt gggtatgat tatgtggag gtgttcagc 
      gggatggac tcgagtggg ccctcctat ggccgattt 
      tgcggctca acgaaacca ttcgatatt ttctcgccg 
      tacgacgcc ctcttggtc cgttttcgc tccgacgac 
      acttttgtg agcaaaggt tttagcata gtttacgaa 
      gccgtcgaa gaggcgttc agtggcgaa gacgaagtg 
      tag
    mRNA sequence
    • >TC034615 RA
      tcgcgttct agttgcaag gcctcgagt aaacacgcg 
      cttctcgat tgtcgtcgt cttcaggag ggaaatgca 
      ggcacgagt cgcgcaagt gatctttgt ccgcttggc 
      caaacatct ctgataatg ccataaacc cagcgcttc 
      cattcgaaa aacgatgtg atttcaccc tagtccagt 
      gttcgcgtc ggaagcatg cagtgtgtg gcggccttt 
      atcagtttg ttgtgtgtc ttgcgtgcc gcgaactgt 
      gataacagg actaagcgg ttcaccgtc gaggacctc 
      ctctccacc accttcccg aagaaggtc tccgacgac 
      caggacctg gacccctgc aaggccggt gggttcatg 
      ggcgacatc gccttgccc atcaagccc aagcccaag 
      gactcgctg ctccaggag gaagtggag aagtacaag 
      caggaggtg ctcgaaggg ctccagatt gaggaagaa 
      gggctcacg gacttcatc cggaagaag accaagcag 
      ccggcgccc atcccaacg ccccggagt tacaacgac 
      ccgtttgat gatatttcc ggagtggcg aatccgacg 
      cacaaaaac ggccaaatt ctcaaaccc gccgccgga 
      tttccgaat ccgaaaaac gatacgaag attcggcat 
      aataagaga catgcggag gtcagacgg agacggaaa 
      cggaatcgg aaaggggat tcagacgag acgccggtt 
      catgatttt gagccgaat tttgatatg aagaggaaa 
      cggccactt ttgaacgaa aattcccac cataggaga 
      gtgacaaga gcggcaacg gcacggaaa gaaagagtc 
      tgggattat ggggtcatt ccctatgaa attgacggg 
      aattttagt ggaggacat aaggcattg ttcaagcag 
      gcgatgagg cattgggag aatttcaca tgtgtcaag 
      tttgtggaa aggaatagg gatgagcac cccaattat 
      attgtcttc acggaaagg ccttgtgga tgttgttct 
      ttcgtcgga aaaaggggc aacggaggc caagcaatc 
      agcattgga aaaaactgc gacaaattc ggaattgtc 
      gtacatgaa ctgggtcac gtggttgga ttttggcat 
      gagcacact agacctgac cgggataaa catgttaat 
      ataattcgg gagaatatt atgagtggt caagaatac 
      aattttaat aaattaaac gatgaagaa gtgaattct 
      ctgggccta atttatgac tacgattca atcatgcat 
      tatgcacga aacacattc tcaaagggg acttattta 
      gatacaatc cagcccata gatgtcccc aataggaag 
      cgtcctgaa atcggacaa agagtccgt ttgagcgaa 
      ggcgatata gctcaaacg aatcttctt tataagtgt 
      attaaatgt gggaggacc tatcaggcc aactcggcc 
      accttctcc tccccctcc tacctcaac cacaacgcc 
      cccgaagac ggcgaaaag tgcgaatgg cggatcaca 
      gcaacccat ggcgagaaa atcgtcttg aacatcacc 
      gacctggac atcgaaaaa tcccccgac tgtggaacc 
      gactacatc gaaatccgc gacggctat tggcacaaa 
      tctcccctt ctgggaaaa ttctgcggc accggaaaa 
      ctcgactcc atcacgtcg accggaagc cgaatgctg 
      atcaactac gtggcgaaa aacccgaac ggccatcgc 
      ggtttcgtg gccaactac gaggccatc tgtgggggc 
      gagctgtac atcgacagt gagggccac ttggagtcc 
      ccaaactac cccgaggag taccaaccc aacaaagag 
      tgcctctgg aagatcaca gttccggag aactaccaa 
      gtggctctc agatttcag tcgttcgaa gtcgagaac 
      cacgatggg tgcgtttac gactacgtg gaaatccgg 
      gacgggttt gcgccggat agtaccatt ctgggcgtg 
      cactgcggg tacaaggtc ccatcggat gtcatctcg 
      agctccaac catctcctg gtcaagttc gtctcggat 
      gggtcggtg cagaaaggc ggcttttcg gccaccatc 
      atgaaggag tacgacgag tgtgccatg atcgaccat 
      gggtgcgcc caggagtgc gtcaacacg ctgggcagc 
      tacgagtgc acctgcaag atcggcttc gagctgcac 
      tccgatggg aaaaactgc gaggatgct tgtggcgga 
      ctcctggac gcgcccaat gggactatc acgtctccg 
      tccttccca gatttgtac ccattgaac aagaactgc 
      gtttgggag attgtgtcc ttgccgcag taccggatt 
      actctaaat ttcacgcat tttgacctg gaggggaat 
      aatatgcac cacgcgcag caacaacag tgcgactac 
      gaccggctg gaaatctac agtaaatta ggggatgaa 
      aagatgaaa aaacacgga gttttttgc gggtcgagg 
      gcccctctt cccatcatt tcagaggga aatgtgatg 
      agaattgtc ttcagttcg gacgttagt gtccaaaag 
      actggcttt gcggctgtt ttcttcaca gatatggac 
      gaatgtgcc ataaacaac ggcgactgc cagcacgaa 
      tgcgtcaac actgtcggt tcctacaaa tgcacctgc 
      cataacggc ttcaccctc cacgaaaac gggcgcgat 
      tgcaaagag ggcgactgc aagtacgaa atctccctc 
      cctttcgga agcatcgga agtccaaac taccccgac 
      tactacccc agtcgcaaa gactgcgtc tggcaattc 
      accacaacc cccggccac cgaatccga atttccttc 
      ctatttttc gaagtcgag ccccatcaa gagtgctac 
      tacgaccac gtggacttc tacgacggc ccttcgccc 
      gagtccagc actttgggg aagttctgc ggctcgaag 
      ttaccccat cctatagtt tcctccggg aatgaactc 
      tatatgacg ttcaaatct gacgcctca gtccagagg 
      aaagggttc tgggccact tatgccgca gtctgtgga 
      gggattctc caagcgacg cccaagaaa aagcacatt 
      tattcccat gcgaagttt gggacggct aattatgac 
      aataaggcc gattgtgat tggacgatt aaggccatt 
      tcggggtat aatgtcaga ctgtcgttt ctgacgttt 
      gatttggag gatgagaag gactgtggg tatgattat 
      gtggaggtg ttcagcggg atggactcg agtgggccc 
      tcctatggc cgattttgc ggctcaacg aaaccattc 
      gatattttc tcgccgtac gacgccctc ttggtccgt 
      tttcgctcc gacgacact tttgtgagc aaaggtttt 
      agcatagtt tacgaagcc gtcgaagag gcgttcagt 
      ggcgaagac gaagtgtag agtccagca gcaaactct 
      tcgagttga ttgtcgttt ggtccagct tcgaataga 
      gggcgcggt gttccaacg aagtgcgaa actcaataa 
      actcagatt tgctcaata tatgaatta ctaggccgt 
      ttctagtat ttatttcaa atattcgta cttatacta 
      gtgaaatgt cttcgtaaa tacctgata ggaggataa 
      tttgtcatt gttcgattt attattaat ggataaaca 
      cttataata aacagactg ccgacttta gattttatg 
      tcacgactc tatggatat tcatatacc gctctatag 
      ctaggttaa cgataggat ttacaagtt gttatcaaa 
      ttcgacgtg ccattttta agttaagga cgctggaat 
      tgtacagat tttcgtgag ctcatgtat acatctctt 
      gtgtaagtt atgaataaa tattatgtc gagta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY