Status
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Gene information
General information
TC034615 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19517246..19541658
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034615
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01822

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection

    Pupal morphology
    wings shortened - Number of animals showing the phenotype: 7

    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 9


    elytra shortened - Number of animals showing the phenotype: 7


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034615 RA
      MQCVAAFIS LLCVLRAAN CDNRTKRFT VEDLLSTTF 
PKKVSDDQD LDPCKAGGF MGDIALPIK PKPKDSLLQ 
EEVEKYKQE VLEGLQIEE EGLTDFIRK KTKQPAPIP 
TPRSYNDPF DDISGVANP THKNGQILK PAAGFPNPK 
NDTKIRHNK RHAEVRRRR KRNRKGDSD ETPVHDFEP 
NFDMKRKRP LLNENSHHR RVTRAATAR KERVWDYGV 
IPYEIDGNF SGGHKALFK QAMRHWENF TCVKFVERN 
RDEHPNYIV FTERPCGCC SFVGKRGNG GQAISIGKN 
CDKFGIVVH ELGHVVGFW HEHTRPDRD KHVNIIREN 
IMSGQEYNF NKLNDEEVN SLGLIYDYD SIMHYARNT 
FSKGTYLDT IQPIDVPNR KRPEIGQRV RLSEGDIAQ 
TNLLYKCIK CGRTYQANS ATFSSPSYL NHNAPEDGE 
KCEWRITAT HGEKIVLNI TDLDIEKSP DCGTDYIEI 
RDGYWHKSP LLGKFCGTG KLDSITSTG SRMLINYVA 
KNPNGHRGF VANYEAICG GELYIDSEG HLESPNYPE 
EYQPNKECL WKITVPENY QVALRFQSF EVENHDGCV 
YDYVEIRDG FAPDSTILG VHCGYKVPS DVISSSNHL 
LVKFVSDGS VQKGGFSAT IMKEYDECA MIDHGCAQE 
CVNTLGSYE CTCKIGFEL HSDGKNCED ACGGLLDAP 
NGTITSPSF PDLYPLNKN CVWEIVSLP QYRITLNFT 
HFDLEGNNM HHAQQQQCD YDRLEIYSK LGDEKMKKH 
GVFCGSRAP LPIISEGNV MRIVFSSDV SVQKTGFAA 
VFFTDMDEC AINNGDCQH ECVNTVGSY KCTCHNGFT 
LHENGRDCK EGDCKYEIS LPFGSIGSP NYPDYYPSR 
KDCVWQFTT TPGHRIRIS FLFFEVEPH QECYYDHVD 
FYDGPSPES STLGKFCGS KLPHPIVSS GNELYMTFK 
SDASVQRKG FWATYAAVC GGILQATPK KKHIYSHAK 
FGTANYDNK ADCDWTIKA ISGYNVRLS FLTFDLEDE 
KDCGYDYVE VFSGMDSSG PSYGRFCGS TKPFDIFSP 
YDALLVRFR SDDTFVSKG FSIVYEAVE EAFSGEDEV
    CDS sequence
    • >TC034615 RA
      atgcagtgt gtggcggcc tttatcagt ttgttgtgt 
gtcttgcgt gccgcgaac tgtgataac aggactaag 
cggttcacc gtcgaggac ctcctctcc accaccttc 
ccgaagaag gtctccgac gaccaggac ctggacccc 
tgcaaggcc ggtgggttc atgggcgac atcgccttg 
cccatcaag cccaagccc aaggactcg ctgctccag 
gaggaagtg gagaagtac aagcaggag gtgctcgaa 
gggctccag attgaggaa gaagggctc acggacttc 
atccggaag aagaccaag cagccggcg cccatccca 
acgccccgg agttacaac gacccgttt gatgatatt 
tccggagtg gcgaatccg acgcacaaa aacggccaa 
attctcaaa cccgccgcc ggatttccg aatccgaaa 
aacgatacg aagattcgg cataataag agacatgcg 
gaggtcaga cggagacgg aaacggaat cggaaaggg 
gattcagac gagacgccg gttcatgat tttgagccg 
aattttgat atgaagagg aaacggcca cttttgaac 
gaaaattcc caccatagg agagtgaca agagcggca 
acggcacgg aaagaaaga gtctgggat tatggggtc 
attccctat gaaattgac gggaatttt agtggagga 
cataaggca ttgttcaag caggcgatg aggcattgg 
gagaatttc acatgtgtc aagtttgtg gaaaggaat 
agggatgag caccccaat tatattgtc ttcacggaa 
aggccttgt ggatgttgt tctttcgtc ggaaaaagg 
ggcaacgga ggccaagca atcagcatt ggaaaaaac 
tgcgacaaa ttcggaatt gtcgtacat gaactgggt 
cacgtggtt ggattttgg catgagcac actagacct 
gaccgggat aaacatgtt aatataatt cgggagaat 
attatgagt ggtcaagaa tacaatttt aataaatta 
aacgatgaa gaagtgaat tctctgggc ctaatttat 
gactacgat tcaatcatg cattatgca cgaaacaca 
ttctcaaag gggacttat ttagataca atccagccc 
atagatgtc cccaatagg aagcgtcct gaaatcgga 
caaagagtc cgtttgagc gaaggcgat atagctcaa 
acgaatctt ctttataag tgtattaaa tgtgggagg 
acctatcag gccaactcg gccaccttc tcctccccc 
tcctacctc aaccacaac gcccccgaa gacggcgaa 
aagtgcgaa tggcggatc acagcaacc catggcgag 
aaaatcgtc ttgaacatc accgacctg gacatcgaa 
aaatccccc gactgtgga accgactac atcgaaatc 
cgcgacggc tattggcac aaatctccc cttctggga 
aaattctgc ggcaccgga aaactcgac tccatcacg 
tcgaccgga agccgaatg ctgatcaac tacgtggcg 
aaaaacccg aacggccat cgcggtttc gtggccaac 
tacgaggcc atctgtggg ggcgagctg tacatcgac 
agtgagggc cacttggag tccccaaac taccccgag 
gagtaccaa cccaacaaa gagtgcctc tggaagatc 
acagttccg gagaactac caagtggct ctcagattt 
cagtcgttc gaagtcgag aaccacgat gggtgcgtt 
tacgactac gtggaaatc cgggacggg tttgcgccg 
gatagtacc attctgggc gtgcactgc gggtacaag 
gtcccatcg gatgtcatc tcgagctcc aaccatctc 
ctggtcaag ttcgtctcg gatgggtcg gtgcagaaa 
ggcggcttt tcggccacc atcatgaag gagtacgac 
gagtgtgcc atgatcgac catgggtgc gcccaggag 
tgcgtcaac acgctgggc agctacgag tgcacctgc 
aagatcggc ttcgagctg cactccgat gggaaaaac 
tgcgaggat gcttgtggc ggactcctg gacgcgccc 
aatgggact atcacgtct ccgtccttc ccagatttg 
tacccattg aacaagaac tgcgtttgg gagattgtg 
tccttgccg cagtaccgg attactcta aatttcacg 
cattttgac ctggagggg aataatatg caccacgcg 
cagcaacaa cagtgcgac tacgaccgg ctggaaatc 
tacagtaaa ttaggggat gaaaagatg aaaaaacac 
ggagttttt tgcgggtcg agggcccct cttcccatc 
atttcagag ggaaatgtg atgagaatt gtcttcagt 
tcggacgtt agtgtccaa aagactggc tttgcggct 
gttttcttc acagatatg gacgaatgt gccataaac 
aacggcgac tgccagcac gaatgcgtc aacactgtc 
ggttcctac aaatgcacc tgccataac ggcttcacc 
ctccacgaa aacgggcgc gattgcaaa gagggcgac 
tgcaagtac gaaatctcc ctccctttc ggaagcatc 
ggaagtcca aactacccc gactactac cccagtcgc 
aaagactgc gtctggcaa ttcaccaca acccccggc 
caccgaatc cgaatttcc ttcctattt ttcgaagtc 
gagccccat caagagtgc tactacgac cacgtggac 
ttctacgac ggcccttcg cccgagtcc agcactttg 
gggaagttc tgcggctcg aagttaccc catcctata 
gtttcctcc gggaatgaa ctctatatg acgttcaaa 
tctgacgcc tcagtccag aggaaaggg ttctgggcc 
acttatgcc gcagtctgt ggagggatt ctccaagcg 
acgcccaag aaaaagcac atttattcc catgcgaag 
tttgggacg gctaattat gacaataag gccgattgt 
gattggacg attaaggcc atttcgggg tataatgtc 
agactgtcg tttctgacg tttgatttg gaggatgag 
aaggactgt gggtatgat tatgtggag gtgttcagc 
gggatggac tcgagtggg ccctcctat ggccgattt 
tgcggctca acgaaacca ttcgatatt ttctcgccg 
tacgacgcc ctcttggtc cgttttcgc tccgacgac 
acttttgtg agcaaaggt tttagcata gtttacgaa 
gccgtcgaa gaggcgttc agtggcgaa gacgaagtg 
tag
    mRNA sequence
    • >TC034615 RA
      tcgcgttct agttgcaag gcctcgagt aaacacgcg 
cttctcgat tgtcgtcgt cttcaggag ggaaatgca 
ggcacgagt cgcgcaagt gatctttgt ccgcttggc 
caaacatct ctgataatg ccataaacc cagcgcttc 
cattcgaaa aacgatgtg atttcaccc tagtccagt 
gttcgcgtc ggaagcatg cagtgtgtg gcggccttt 
atcagtttg ttgtgtgtc ttgcgtgcc gcgaactgt 
gataacagg actaagcgg ttcaccgtc gaggacctc 
ctctccacc accttcccg aagaaggtc tccgacgac 
caggacctg gacccctgc aaggccggt gggttcatg 
ggcgacatc gccttgccc atcaagccc aagcccaag 
gactcgctg ctccaggag gaagtggag aagtacaag 
caggaggtg ctcgaaggg ctccagatt gaggaagaa 
gggctcacg gacttcatc cggaagaag accaagcag 
ccggcgccc atcccaacg ccccggagt tacaacgac 
ccgtttgat gatatttcc ggagtggcg aatccgacg 
cacaaaaac ggccaaatt ctcaaaccc gccgccgga 
tttccgaat ccgaaaaac gatacgaag attcggcat 
aataagaga catgcggag gtcagacgg agacggaaa 
cggaatcgg aaaggggat tcagacgag acgccggtt 
catgatttt gagccgaat tttgatatg aagaggaaa 
cggccactt ttgaacgaa aattcccac cataggaga 
gtgacaaga gcggcaacg gcacggaaa gaaagagtc 
tgggattat ggggtcatt ccctatgaa attgacggg 
aattttagt ggaggacat aaggcattg ttcaagcag 
gcgatgagg cattgggag aatttcaca tgtgtcaag 
tttgtggaa aggaatagg gatgagcac cccaattat 
attgtcttc acggaaagg ccttgtgga tgttgttct 
ttcgtcgga aaaaggggc aacggaggc caagcaatc 
agcattgga aaaaactgc gacaaattc ggaattgtc 
gtacatgaa ctgggtcac gtggttgga ttttggcat 
gagcacact agacctgac cgggataaa catgttaat 
ataattcgg gagaatatt atgagtggt caagaatac 
aattttaat aaattaaac gatgaagaa gtgaattct 
ctgggccta atttatgac tacgattca atcatgcat 
tatgcacga aacacattc tcaaagggg acttattta 
gatacaatc cagcccata gatgtcccc aataggaag 
cgtcctgaa atcggacaa agagtccgt ttgagcgaa 
ggcgatata gctcaaacg aatcttctt tataagtgt 
attaaatgt gggaggacc tatcaggcc aactcggcc 
accttctcc tccccctcc tacctcaac cacaacgcc 
cccgaagac ggcgaaaag tgcgaatgg cggatcaca 
gcaacccat ggcgagaaa atcgtcttg aacatcacc 
gacctggac atcgaaaaa tcccccgac tgtggaacc 
gactacatc gaaatccgc gacggctat tggcacaaa 
tctcccctt ctgggaaaa ttctgcggc accggaaaa 
ctcgactcc atcacgtcg accggaagc cgaatgctg 
atcaactac gtggcgaaa aacccgaac ggccatcgc 
ggtttcgtg gccaactac gaggccatc tgtgggggc 
gagctgtac atcgacagt gagggccac ttggagtcc 
ccaaactac cccgaggag taccaaccc aacaaagag 
tgcctctgg aagatcaca gttccggag aactaccaa 
gtggctctc agatttcag tcgttcgaa gtcgagaac 
cacgatggg tgcgtttac gactacgtg gaaatccgg 
gacgggttt gcgccggat agtaccatt ctgggcgtg 
cactgcggg tacaaggtc ccatcggat gtcatctcg 
agctccaac catctcctg gtcaagttc gtctcggat 
gggtcggtg cagaaaggc ggcttttcg gccaccatc 
atgaaggag tacgacgag tgtgccatg atcgaccat 
gggtgcgcc caggagtgc gtcaacacg ctgggcagc 
tacgagtgc acctgcaag atcggcttc gagctgcac 
tccgatggg aaaaactgc gaggatgct tgtggcgga 
ctcctggac gcgcccaat gggactatc acgtctccg 
tccttccca gatttgtac ccattgaac aagaactgc 
gtttgggag attgtgtcc ttgccgcag taccggatt 
actctaaat ttcacgcat tttgacctg gaggggaat 
aatatgcac cacgcgcag caacaacag tgcgactac 
gaccggctg gaaatctac agtaaatta ggggatgaa 
aagatgaaa aaacacgga gttttttgc gggtcgagg 
gcccctctt cccatcatt tcagaggga aatgtgatg 
agaattgtc ttcagttcg gacgttagt gtccaaaag 
actggcttt gcggctgtt ttcttcaca gatatggac 
gaatgtgcc ataaacaac ggcgactgc cagcacgaa 
tgcgtcaac actgtcggt tcctacaaa tgcacctgc 
cataacggc ttcaccctc cacgaaaac gggcgcgat 
tgcaaagag ggcgactgc aagtacgaa atctccctc 
cctttcgga agcatcgga agtccaaac taccccgac 
tactacccc agtcgcaaa gactgcgtc tggcaattc 
accacaacc cccggccac cgaatccga atttccttc 
ctatttttc gaagtcgag ccccatcaa gagtgctac 
tacgaccac gtggacttc tacgacggc ccttcgccc 
gagtccagc actttgggg aagttctgc ggctcgaag 
ttaccccat cctatagtt tcctccggg aatgaactc 
tatatgacg ttcaaatct gacgcctca gtccagagg 
aaagggttc tgggccact tatgccgca gtctgtgga 
gggattctc caagcgacg cccaagaaa aagcacatt 
tattcccat gcgaagttt gggacggct aattatgac 
aataaggcc gattgtgat tggacgatt aaggccatt 
tcggggtat aatgtcaga ctgtcgttt ctgacgttt 
gatttggag gatgagaag gactgtggg tatgattat 
gtggaggtg ttcagcggg atggactcg agtgggccc 
tcctatggc cgattttgc ggctcaacg aaaccattc 
gatattttc tcgccgtac gacgccctc ttggtccgt 
tttcgctcc gacgacact tttgtgagc aaaggtttt 
agcatagtt tacgaagcc gtcgaagag gcgttcagt 
ggcgaagac gaagtgtag agtccagca gcaaactct 
tcgagttga ttgtcgttt ggtccagct tcgaataga 
gggcgcggt gttccaacg aagtgcgaa actcaataa 
actcagatt tgctcaata tatgaatta ctaggccgt 
ttctagtat ttatttcaa atattcgta cttatacta 
gtgaaatgt cttcgtaaa tacctgata ggaggataa 
tttgtcatt gttcgattt attattaat ggataaaca 
cttataata aacagactg ccgacttta gattttatg 
tcacgactc tatggatat tcatatacc gctctatag 
ctaggttaa cgataggat ttacaagtt gttatcaaa 
ttcgacgtg ccattttta agttaagga cgctggaat 
tgtacagat tttcgtgag ctcatgtat acatctctt 
gtgtaagtt atgaataaa tattatgtc gagta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY
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