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Gene information
General information
TC011185 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3132321..3135372
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011185
    iBeetle screen iB_01821

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011185 RA
      MAVRICIEI GHEASLRTK KTQEGFTHD WEVFVRGCD 
      GAEIHYYIE KVVFYLHET FPKPKRVVK EPPYSVKES 
      GYAGFNFPI DIYLRNNNE PKKIRFTYD LTLQQSGPP 
      IVKVQKEKY VFTSVSDEF KMKLLKGGA TIGSSSSMQ 
      DVSDNRSSI DDKNQMTNK PKLSGSDMK KQKIKIGEP 
      CRDFQELFG SPINMSKSS EPKLTIPKP DSKGVEKSR 
      TKHSPHKES HKDKEKSKD KTDSEKKNR DEKKKERSK 
      DHERSKDRS TKKEKSPRP KSPSPKRSP KRSSSPRFS 
      SPPVLKKES KHNDKEKRD KNEERKSKK EKRSKEHKE 
      NKHGEGEQK TDEHKDNKI KSEAKKESG KMKSENSAS 
      PTHRFPETE KKKEKPKEK SSKESSKED KYSEKYKHK 
      KKDKEKREK KEEKSYKSE KKSEKIPSE TKPNLFKSE 
      PQVKKEKVY VSPVRPAVE DESSNSSTT SSNSSLVEK 
      KAKTEVVKV KEEKNQKLK KEKGYQENT IKCKRKSDS 
      VEVEEPPEK IHKDHGINN NSDLEQESK AVLPTATEE 
      EEDYFGLLR DLQHKIMGL KDNSDLQKV VKLIAETGR 
      YEVSAHTFD FDLCLLDRS TVQQLQDFF ANET
    CDS sequence
    • >TC011185 RA
      atggcagtg cgaatttgc atcgaaata ggtcacgag 
      gcatctctc cgtaccaaa aagacccaa gaaggcttc 
      acccatgac tgggaggtg ttcgtgcga gggtgcgac 
      ggtgcggag attcactat tacattgaa aaagttgtc 
      ttctatttg cacgagacg tttccgaag ccaaaaagg 
      gtggtcaag gagcccccg tattccgtg aaggagtca 
      gggtacgct gggttcaac ttccccatc gatatttat 
      ttaaggaac aataatgag ccgaagaaa atcaggttt 
      acgtacgat ttgaccttg caacagtca gggccccct 
      attgttaaa gtgcagaag gaaaagtat gtcttcact 
      tcggtcagt gatgaattc aagatgaaa ttgttaaaa 
      gggggtgca acgatcggg agctcctct agcatgcaa 
      gatgtgagc gataacaga tcgtcaatt gatgataag 
      aatcaaatg actaataaa ccgaaattg agcggttcc 
      gacatgaag aagcaaaaa atcaaaatt ggggaacct 
      tgtagggac ttccaggaa ttgtttggc tcccctatc 
      aatatgtcc aagagttca gaacctaaa ttaacaatt 
      cctaaacct gacagtaaa ggtgtggag aagtcccgg 
      acgaaacac agcccccat aaggagagt cacaaggac 
      aaggaaaaa tcgaaggat aagaccgat tcggagaaa 
      aaaaatcgc gatgaaaag aagaaagaa cgatcaaag 
      gatcacgaa cgaagtaaa gacagaagt accaaaaag 
      gaaaaatcc ccgcgcccc aaaagtcct agtccgaaa 
      agatcacca aaacggtcg tccagtcct cgattttct 
      agtccaccg gttttaaaa aaggaatcg aaacacaac 
      gataaagaa aaaagggac aaaaacgaa gaacgaaag 
      tcgaagaaa gagaaacgg agtaaagaa cacaaagag 
      aacaaacac ggagaaggc gaacaaaaa acggacgag 
      cacaaggat aataaaatc aaaagtgag gcgaagaaa 
      gaaagtgga aaaatgaaa agcgagaat tcggcgtcg 
      ccaacacat cggtttcct gaaactgaa aaaaagaaa 
      gagaagccg aaggagaag tcgtcaaag gagtcgtcg 
      aaagaggac aaatactcg gagaagtat aagcacaag 
      aagaaagac aaagaaaag cgcgagaag aaggaggaa 
      aagtcgtac aagtctgag aaaaagtct gagaaaatt 
      ccaagcgaa accaaaccg aatttattc aaaagtgag 
      cctcaagtg aagaaggag aaagtttat gttagtcca 
      gttcggcct gcggtcgaa gatgagtcc tcgaatagt 
      tcaacaact agttcgaat tcgtcacta gttgagaaa 
      aaggccaaa actgaagtg gtgaaagtc aaggaagag 
      aaaaatcaa aaattgaag aaggaaaaa gggtatcaa 
      gagaacaca attaagtgt aagcgaaaa tcggattcg 
      gtggaagtg gaggaacca ccggaaaag attcataaa 
      gatcatggg attaataat aactcggat ttggagcag 
      gagagtaag gcagtgctg ccaacagcg accgaggag 
      gaggaggac tacttcgga ttattgcgt gacctccaa 
      cacaaaata atgggactc aaggacaat tctgatctc 
      caaaaagtc gtgaaactg atcgccgag actggccgt 
      tacgaagta tcagctcac acttttgat tttgatttg 
      tgtttgttg gacagatca acagtccaa caactacaa 
      gactttttt gcgaatgaa acttaa
    mRNA sequence
    • >TC011185 RA
      actgtgtat tgttttctg agccccgtg tcatcagca 
      ccccgaaac ggcgtcaaa atgggttaa aaccagcac 
      cccagtacc atggcagtg cgaatttgc atcgaaata 
      ggtcacgag gcatctctc cgtaccaaa aagacccaa 
      gaaggcttc acccatgac tgggaggtg ttcgtgcga 
      gggtgcgac ggtgcggag attcactat tacattgaa 
      aaagttgtc ttctatttg cacgagacg tttccgaag 
      ccaaaaagg gtggtcaag gagcccccg tattccgtg 
      aaggagtca gggtacgct gggttcaac ttccccatc 
      gatatttat ttaaggaac aataatgag ccgaagaaa 
      atcaggttt acgtacgat ttgaccttg caacagtca 
      gggccccct attgttaaa gtgcagaag gaaaagtat 
      gtcttcact tcggtcagt gatgaattc aagatgaaa 
      ttgttaaaa gggggtgca acgatcggg agctcctct 
      agcatgcaa gatgtgagc gataacaga tcgtcaatt 
      gatgataag aatcaaatg actaataaa ccgaaattg 
      agcggttcc gacatgaag aagcaaaaa atcaaaatt 
      ggggaacct tgtagggac ttccaggaa ttgtttggc 
      tcccctatc aatatgtcc aagagttca gaacctaaa 
      ttaacaatt cctaaacct gacagtaaa ggtgtggag 
      aagtcccgg acgaaacac agcccccat aaggagagt 
      cacaaggac aaggaaaaa tcgaaggat aagaccgat 
      tcggagaaa aaaaatcgc gatgaaaag aagaaagaa 
      cgatcaaag gatcacgaa cgaagtaaa gacagaagt 
      accaaaaag gaaaaatcc ccgcgcccc aaaagtcct 
      agtccgaaa agatcacca aaacggtcg tccagtcct 
      cgattttct agtccaccg gttttaaaa aaggaatcg 
      aaacacaac gataaagaa aaaagggac aaaaacgaa 
      gaacgaaag tcgaagaaa gagaaacgg agtaaagaa 
      cacaaagag aacaaacac ggagaaggc gaacaaaaa 
      acggacgag cacaaggat aataaaatc aaaagtgag 
      gcgaagaaa gaaagtgga aaaatgaaa agcgagaat 
      tcggcgtcg ccaacacat cggtttcct gaaactgaa 
      aaaaagaaa gagaagccg aaggagaag tcgtcaaag 
      gagtcgtcg aaagaggac aaatactcg gagaagtat 
      aagcacaag aagaaagac aaagaaaag cgcgagaag 
      aaggaggaa aagtcgtac aagtctgag aaaaagtct 
      gagaaaatt ccaagcgaa accaaaccg aatttattc 
      aaaagtgag cctcaagtg aagaaggag aaagtttat 
      gttagtcca gttcggcct gcggtcgaa gatgagtcc 
      tcgaatagt tcaacaact agttcgaat tcgtcacta 
      gttgagaaa aaggccaaa actgaagtg gtgaaagtc 
      aaggaagag aaaaatcaa aaattgaag aaggaaaaa 
      gggtatcaa gagaacaca attaagtgt aagcgaaaa 
      tcggattcg gtggaagtg gaggaacca ccggaaaag 
      attcataaa gatcatggg attaataat aactcggat 
      ttggagcag gagagtaag gcagtgctg ccaacagcg 
      accgaggag gaggaggac tacttcgga ttattgcgt 
      gacctccaa cacaaaata atgggactc aaggacaat 
      tctgatctc caaaaagtc gtgaaactg atcgccgag 
      actggccgt tacgaagta tcagctcac acttttgat 
      tttgatttg tgtttgttg gacagatca acagtccaa 
      caactacaa gactttttt gcgaatgaa acttaatta 
      actttaggt tgcaatatt ttagtttta ggacaattt 
      ccccgatgt ttgcggaaa agtgtcgtc ttttgttat 
      gtacctgtt ttaaggagt ttttggatg tttatttct 
      cctggtagg agagtgcat ttcgttgtg gttttctgt 
      tgtttttac acattttat tgtacttgt acagtgata 
      aatgctatt atttttgta attttcaaa tcgtagcga 
      actaatgta accaccgtt tcgtttttg aacgtaggt 
      atattgtta tctagcaga ttagtgtaa caaggatca 
      gtgttgaat aagcattac taggtttaa gtttttaat 
      gaacctgtt attatacat ttatttaat tgttttcgt 
      aatactaat gccggacct gtaatttat ttgaccgtc 
      ttagcaata aataaatgt aaaattaaa aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY