Status
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Gene information
General information
TC011185 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3132321..3135372
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011185
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01821

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011185 RA
      MAVRICIEI GHEASLRTK KTQEGFTHD WEVFVRGCD 
GAEIHYYIE KVVFYLHET FPKPKRVVK EPPYSVKES 
GYAGFNFPI DIYLRNNNE PKKIRFTYD LTLQQSGPP 
IVKVQKEKY VFTSVSDEF KMKLLKGGA TIGSSSSMQ 
DVSDNRSSI DDKNQMTNK PKLSGSDMK KQKIKIGEP 
CRDFQELFG SPINMSKSS EPKLTIPKP DSKGVEKSR 
TKHSPHKES HKDKEKSKD KTDSEKKNR DEKKKERSK 
DHERSKDRS TKKEKSPRP KSPSPKRSP KRSSSPRFS 
SPPVLKKES KHNDKEKRD KNEERKSKK EKRSKEHKE 
NKHGEGEQK TDEHKDNKI KSEAKKESG KMKSENSAS 
PTHRFPETE KKKEKPKEK SSKESSKED KYSEKYKHK 
KKDKEKREK KEEKSYKSE KKSEKIPSE TKPNLFKSE 
PQVKKEKVY VSPVRPAVE DESSNSSTT SSNSSLVEK 
KAKTEVVKV KEEKNQKLK KEKGYQENT IKCKRKSDS 
VEVEEPPEK IHKDHGINN NSDLEQESK AVLPTATEE 
EEDYFGLLR DLQHKIMGL KDNSDLQKV VKLIAETGR 
YEVSAHTFD FDLCLLDRS TVQQLQDFF ANET
    CDS sequence
    • >TC011185 RA
      atggcagtg cgaatttgc atcgaaata ggtcacgag 
gcatctctc cgtaccaaa aagacccaa gaaggcttc 
acccatgac tgggaggtg ttcgtgcga gggtgcgac 
ggtgcggag attcactat tacattgaa aaagttgtc 
ttctatttg cacgagacg tttccgaag ccaaaaagg 
gtggtcaag gagcccccg tattccgtg aaggagtca 
gggtacgct gggttcaac ttccccatc gatatttat 
ttaaggaac aataatgag ccgaagaaa atcaggttt 
acgtacgat ttgaccttg caacagtca gggccccct 
attgttaaa gtgcagaag gaaaagtat gtcttcact 
tcggtcagt gatgaattc aagatgaaa ttgttaaaa 
gggggtgca acgatcggg agctcctct agcatgcaa 
gatgtgagc gataacaga tcgtcaatt gatgataag 
aatcaaatg actaataaa ccgaaattg agcggttcc 
gacatgaag aagcaaaaa atcaaaatt ggggaacct 
tgtagggac ttccaggaa ttgtttggc tcccctatc 
aatatgtcc aagagttca gaacctaaa ttaacaatt 
cctaaacct gacagtaaa ggtgtggag aagtcccgg 
acgaaacac agcccccat aaggagagt cacaaggac 
aaggaaaaa tcgaaggat aagaccgat tcggagaaa 
aaaaatcgc gatgaaaag aagaaagaa cgatcaaag 
gatcacgaa cgaagtaaa gacagaagt accaaaaag 
gaaaaatcc ccgcgcccc aaaagtcct agtccgaaa 
agatcacca aaacggtcg tccagtcct cgattttct 
agtccaccg gttttaaaa aaggaatcg aaacacaac 
gataaagaa aaaagggac aaaaacgaa gaacgaaag 
tcgaagaaa gagaaacgg agtaaagaa cacaaagag 
aacaaacac ggagaaggc gaacaaaaa acggacgag 
cacaaggat aataaaatc aaaagtgag gcgaagaaa 
gaaagtgga aaaatgaaa agcgagaat tcggcgtcg 
ccaacacat cggtttcct gaaactgaa aaaaagaaa 
gagaagccg aaggagaag tcgtcaaag gagtcgtcg 
aaagaggac aaatactcg gagaagtat aagcacaag 
aagaaagac aaagaaaag cgcgagaag aaggaggaa 
aagtcgtac aagtctgag aaaaagtct gagaaaatt 
ccaagcgaa accaaaccg aatttattc aaaagtgag 
cctcaagtg aagaaggag aaagtttat gttagtcca 
gttcggcct gcggtcgaa gatgagtcc tcgaatagt 
tcaacaact agttcgaat tcgtcacta gttgagaaa 
aaggccaaa actgaagtg gtgaaagtc aaggaagag 
aaaaatcaa aaattgaag aaggaaaaa gggtatcaa 
gagaacaca attaagtgt aagcgaaaa tcggattcg 
gtggaagtg gaggaacca ccggaaaag attcataaa 
gatcatggg attaataat aactcggat ttggagcag 
gagagtaag gcagtgctg ccaacagcg accgaggag 
gaggaggac tacttcgga ttattgcgt gacctccaa 
cacaaaata atgggactc aaggacaat tctgatctc 
caaaaagtc gtgaaactg atcgccgag actggccgt 
tacgaagta tcagctcac acttttgat tttgatttg 
tgtttgttg gacagatca acagtccaa caactacaa 
gactttttt gcgaatgaa acttaa
    mRNA sequence
    • >TC011185 RA
      actgtgtat tgttttctg agccccgtg tcatcagca 
ccccgaaac ggcgtcaaa atgggttaa aaccagcac 
cccagtacc atggcagtg cgaatttgc atcgaaata 
ggtcacgag gcatctctc cgtaccaaa aagacccaa 
gaaggcttc acccatgac tgggaggtg ttcgtgcga 
gggtgcgac ggtgcggag attcactat tacattgaa 
aaagttgtc ttctatttg cacgagacg tttccgaag 
ccaaaaagg gtggtcaag gagcccccg tattccgtg 
aaggagtca gggtacgct gggttcaac ttccccatc 
gatatttat ttaaggaac aataatgag ccgaagaaa 
atcaggttt acgtacgat ttgaccttg caacagtca 
gggccccct attgttaaa gtgcagaag gaaaagtat 
gtcttcact tcggtcagt gatgaattc aagatgaaa 
ttgttaaaa gggggtgca acgatcggg agctcctct 
agcatgcaa gatgtgagc gataacaga tcgtcaatt 
gatgataag aatcaaatg actaataaa ccgaaattg 
agcggttcc gacatgaag aagcaaaaa atcaaaatt 
ggggaacct tgtagggac ttccaggaa ttgtttggc 
tcccctatc aatatgtcc aagagttca gaacctaaa 
ttaacaatt cctaaacct gacagtaaa ggtgtggag 
aagtcccgg acgaaacac agcccccat aaggagagt 
cacaaggac aaggaaaaa tcgaaggat aagaccgat 
tcggagaaa aaaaatcgc gatgaaaag aagaaagaa 
cgatcaaag gatcacgaa cgaagtaaa gacagaagt 
accaaaaag gaaaaatcc ccgcgcccc aaaagtcct 
agtccgaaa agatcacca aaacggtcg tccagtcct 
cgattttct agtccaccg gttttaaaa aaggaatcg 
aaacacaac gataaagaa aaaagggac aaaaacgaa 
gaacgaaag tcgaagaaa gagaaacgg agtaaagaa 
cacaaagag aacaaacac ggagaaggc gaacaaaaa 
acggacgag cacaaggat aataaaatc aaaagtgag 
gcgaagaaa gaaagtgga aaaatgaaa agcgagaat 
tcggcgtcg ccaacacat cggtttcct gaaactgaa 
aaaaagaaa gagaagccg aaggagaag tcgtcaaag 
gagtcgtcg aaagaggac aaatactcg gagaagtat 
aagcacaag aagaaagac aaagaaaag cgcgagaag 
aaggaggaa aagtcgtac aagtctgag aaaaagtct 
gagaaaatt ccaagcgaa accaaaccg aatttattc 
aaaagtgag cctcaagtg aagaaggag aaagtttat 
gttagtcca gttcggcct gcggtcgaa gatgagtcc 
tcgaatagt tcaacaact agttcgaat tcgtcacta 
gttgagaaa aaggccaaa actgaagtg gtgaaagtc 
aaggaagag aaaaatcaa aaattgaag aaggaaaaa 
gggtatcaa gagaacaca attaagtgt aagcgaaaa 
tcggattcg gtggaagtg gaggaacca ccggaaaag 
attcataaa gatcatggg attaataat aactcggat 
ttggagcag gagagtaag gcagtgctg ccaacagcg 
accgaggag gaggaggac tacttcgga ttattgcgt 
gacctccaa cacaaaata atgggactc aaggacaat 
tctgatctc caaaaagtc gtgaaactg atcgccgag 
actggccgt tacgaagta tcagctcac acttttgat 
tttgatttg tgtttgttg gacagatca acagtccaa 
caactacaa gactttttt gcgaatgaa acttaatta 
actttaggt tgcaatatt ttagtttta ggacaattt 
ccccgatgt ttgcggaaa agtgtcgtc ttttgttat 
gtacctgtt ttaaggagt ttttggatg tttatttct 
cctggtagg agagtgcat ttcgttgtg gttttctgt 
tgtttttac acattttat tgtacttgt acagtgata 
aatgctatt atttttgta attttcaaa tcgtagcga 
actaatgta accaccgtt tcgtttttg aacgtaggt 
atattgtta tctagcaga ttagtgtaa caaggatca 
gtgttgaat aagcattac taggtttaa gtttttaat 
gaacctgtt attatacat ttatttaat tgttttcgt 
aatactaat gccggacct gtaatttat ttgaccgtc 
ttagcaata aataaatgt aaaattaaa aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY