Status
Please wait ...
Gene information
General information
TC011177 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3198859..3199972
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011177
    iBeetle screen iB_01819

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011177 RA
      MYVFWWFFA YYLRPIVKW FLRKTTGLC ELQRICYGK 
      VQGAPRTKA VDYSLCMSK SPQIAQLIA HLNDVSDNQ 
      RFVGSNLKE LTNGAVNTV MLVKKINPK IHFQFVINF 
      GQCVEQIWG YRQLLAEVE ELRKTVYDA DNFGHERKL 
      QDLWEKLMP HERLEGRVT KQWQYIGFQ GDDPKTDFR 
      GMGLLGLEN LLAFASDYQ DAATYVLSH SHHPHYGYA 
      FAIVGINLT SLAWTLLKQ GDAKTYFFN MVKSAPSLK 
      LFHQFYSYL FYEFDKYWI ECKPKDIME FSTIKDNFE 
      INVRNALQD PNAVFRINF SVDTI
    CDS sequence
    • >TC011177 RA
      atgtacgtt ttttggtgg tttttcgcg tactatttg 
      cgccccatc gtcaaatgg ttccttcgc aagaccacc 
      ggtttgtgc gagcttcag aggatctgc tatggcaag 
      gttcaaggg gcccccagg accaaagcc gtggactac 
      tccctctgc atgtcaaag tcgccccaa atcgcccaa 
      ctgatcgcg cacttgaac gacgtaagc gacaatcag 
      cggtttgtc ggttccaac ctcaaagaa ctcacaaat 
      ggggctgtc aacacagtc atgctagtc aagaaaatc 
      aacccgaaa atccacttc caattcgtg ataaacttc 
      ggtcagtgc gtggagcag atttggggc tatcgccag 
      ctgctggca gaagtggag gaactgagg aagacagtt 
      tacgacgct gataatttt ggacacgaa cgaaaattg 
      caggatttg tgggagaag ttgatgccc cacgagcgt 
      ctggagggg cgcgtgacc aagcagtgg cagtacatc 
      gggttccag ggcgatgac cccaagacc gattttcgg 
      ggaatgggg ctcctgggg ttggagaat ctccttgct 
      ttcgcgagt gattatcag gacgctgcg acttatgtc 
      ctgtctcat tcgcatcac ccgcattat gggtacgcg 
      tttgccatt gtggggatt aatttgaca agtctggcg 
      tggaccctt ttgaaacag ggcgatgcc aagacttat 
      tttttcaat atggtgaaa agtgcacca agtttgaaa 
      ctgtttcac cagttttat agttacttg ttttacgag 
      tttgataaa tattggatt gagtgtaag ccaaaggat 
      attatggag ttttctact attaaggac aactttgaa 
      ataaatgtt aggaatgcg ttgcaggat ccgaatgct 
      gtgtttcgg attaatttt agtgttgac actatttaa
    mRNA sequence
    • >TC011177 RA
      agtcgtcac ccgccgttg tcatgccgc tcaacatgt 
      aaacaatca atttgattg aatttttcc taaattctt 
      gaataaaat gtacgtttt ttggtggtt tttcgcgta 
      ctatttgcg ccccatcgt caaatggtt ccttcgcaa 
      gaccaccgg tttgtgcga gcttcagag gatctgcta 
      tggcaaggt tcaaggggc ccccaggac caaagccgt 
      ggactactc cctctgcat gtcaaagtc gccccaaat 
      cgcccaact gatcgcgca cttgaacga cgtaagcga 
      caatcagcg gtttgtcgg ttccaacct caaagaact 
      cacaaatgg ggctgtcaa cacagtcat gctagtcaa 
      gaaaatcaa cccgaaaat ccacttcca attcgtgat 
      aaacttcgg tcagtgcgt ggagcagat ttggggcta 
      tcgccagct gctggcaga agtggagga actgaggaa 
      gacagttta cgacgctga taattttgg acacgaacg 
      aaaattgca ggatttgtg ggagaagtt gatgcccca 
      cgagcgtct ggaggggcg cgtgaccaa gcagtggca 
      gtacatcgg gttccaggg cgatgaccc caagaccga 
      ttttcgggg aatggggct cctggggtt ggagaatct 
      ccttgcttt cgcgagtga ttatcagga cgctgcgac 
      ttatgtcct gtctcattc gcatcaccc gcattatgg 
      gtacgcgtt tgccattgt ggggattaa tttgacaag 
      tctggcgtg gaccctttt gaaacaggg cgatgccaa 
      gacttattt tttcaatat ggtgaaaag tgcaccaag 
      tttgaaact gtttcacca gttttatag ttacttgtt 
      ttacgagtt tgataaata ttggattga gtgtaagcc 
      aaaggatat tatggagtt ttctactat taaggacaa 
      ctttgaaat aaatgttag gaatgcgtt gcaggatcc 
      gaatgctgt gtttcggat taattttag tgttgacac 
      tatttaagc tctcttgtg atacttcaa ggctttgta 
      aatacgatt tttatgtgt ggtacactt tgtatgttt 
      taagtacac aaacaaata aaagtgaat aaaataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY