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Gene information
General information
TC011176 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3204315..3207892
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC011176
      iBeetle screen iB_01818

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      remarks: later than schedule,
      Pupal morphology
      remarks: later than schedule,
      Fertility
      ovary without detectable defects - Number of animals showing the phenotype: 3
      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
       egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection

      pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection

      eclosion delayed (still pupa) - Number of animals showing the phenotype: 5 (16 dpi)
      Days post injection

      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
      Days post injection

      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (19 dpi)
      Days post injection

      Adult morphology
      elytra dorsal not closed - Number of animals showing the phenotype: 2


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC011176 RA
        MDYFYGAPR RPPAPPTIN RLDRVPIVP EVSIIENPP 
VRPQNVLNI DKSRLRPSF PCDNLKKQV RFQSPNFDN 
KENQLPQPQ TLKTAPDLN GKTFKQIYM ANVPDRHLD 
LSLFEPSFC EKRAQEPQL PQLETVSNY AEFKRQSQT 
CSEFSRLKS IENVEPQIP QRETVNNYA EFRQRQSHL 
RSIENVGFR DSYPSEVRP RHTEICTQT EPPCTEIAP 
TSDEPTIRD LFKIIQQQN EQIMLLQRQ VNNLKTSIR 
QPEIESGPH DDLNNSPKK SLFSFDVKA TSFEFSFRP 
QHNKFRKQN DFLEPRIQE IVENCAENP NSLRLEESL 
NVRDSYVSE VPSIQIKMD EYHSSDDEE ESQGNNNVG 
LTFYKDLMD QVHNVLKKV QKQSECQNR PGQTMKAVK 
QATLQHLKD IGVNLSPIQ ESSEVTRES DCDQTDVSC 
AVQQLLMKY LPEDKLAKA INKPRPIKD NQFKPPLNT 
DFSFATLQY MKKF
      CDS sequence
      • >TC011176 RA
        atggattat ttttacggg gccccccga cggccccca 
gccccccca acaataaac agacttgat agagtacca 
atagtgcct gaagtctca ataattgaa aacccccca 
gtaagaccc caaaacgta ctaaacatc gacaaatct 
cgactcaga ccatcattt ccttgcgat aatttgaaa 
aaacaagtg cggtttcaa agccccaac tttgataat 
aaagaaaac caattgcct caaccacag acgcttaaa 
acagcgcct gatttaaac ggcaagact ttcaagcag 
atttatatg gcaaatgtg cccgaccgg cacttggat 
ctgagcctt tttgagcct tccttctgc gaaaaacgg 
gcacaagag ccccaatta ccacaactt gaaactgtc 
tctaattat gccgaattt aaaagacag tcacaaacg 
tgcagtgaa ttttcacgc ctgaaaagt attgaaaat 
gtcgagccc caaatacca caacgtgaa actgtcaat 
aattatgcc gaattcagg caaagacag tcacacctg 
agaagtatc gaaaatgtt ggctttagg gacagttat 
ccgagcgaa gtgagacca agacacaca gaaatctgc 
actcagact gagccccct tgcaccgaa atcgccccc 
acgagtgac gagcccacg attcgagac ttgttcaaa 
ataatccag caacaaaac gaacaaatt atgttgctc 
caaaggcag gtaaacaac ctgaaaact agcataaga 
cagcccgag atcgaatca gggccccac gacgacttg 
aacaacagc cccaagaag agtttgttt tcgtttgac 
gtcaaagcg acaagtttt gaattttcg ttccggccc 
caacataat aaatttcga aaacaaaac gattttctt 
gagccaagg attcaggaa attgtcgaa aattgcgcc 
gaaaatccg aattcgttg cgactagaa gagtcgcta 
aatgtgaga gatagctac gttagtgaa gtgccgagt 
attcagatt aaaatggat gagtatcac agttcagat 
gacgaggag gagtctcag ggtaataat aacgttggg 
cttacgttc tataaagac ttaatggat caggtccat 
aatgttttg aaaaaagtc caaaaacag tcggagtgt 
caaaacagg cctggccaa actatgaaa gctgtgaaa 
caagcaact ttgcaacat ttgaaagac attggcgtg 
aatttatcg ccaatacag gaatctagt gaggtcaca 
agagaaagt gattgtgac caaaccgat gtcagttgt 
gccgtccaa caactccta atgaaatat ttacctgaa 
gataagttg gccaaagca ataaataaa cctcgtcca 
atcaaagac aaccaattc aagccgccc ctaaacact 
gacttttca ttcgcaaca cttcaatac atgaaaaaa 
ttttag
      mRNA sequence
      • >TC011176 RA
        aagtttacc accacaaat tttttataa atttgcttt 
aaaaaacgt tcctggaca attattttt ataatgatc 
agcgtttct ataatttac ggttgtccg gttctgttc 
tgtacgttt ctgtctatg tccttttct gtaaatgta 
cttgacaag ttccccaaa cattttgac agtagtgac 
agttacttt tttgaattg tgatggatt atttttacg 
gggcccccc gacggcccc cagcccccc caacaataa 
acagacttg atagagtac caatagtgc ctgaagtct 
caataattg aaaaccccc cagtaagac cccaaaacg 
tactaaaca tcgacaaat ctcgactca gaccatcat 
ttccttgcg ataatttga aaaaacaag tgcggtttc 
aaagcccca actttgata ataaagaaa accaattgc 
ctcaaccac agacgctta aaacagcgc ctgatttaa 
acggcaaga ctttcaagc agatttata tggcaaatg 
tgcccgacc ggcacttgg atctgagcc tttttgagc 
cttccttct gcgaaaaac gggcacaag agccccaat 
taccacaac ttgaaactg tctctaatt atgccgaat 
ttaaaagac agtcacaaa cgtgcagtg aattttcac 
gcctgaaaa gtattgaaa atgtcgagc cccaaatac 
cacaacgtg aaactgtca ataattatg ccgaattca 
ggcaaagac agtcacacc tgagaagta tcgaaaatg 
ttggcttta gggacagtt atccgagcg aagtgagac 
caagacaca cagaaatct gcactcaga ctgagcccc 
cttgcaccg aaatcgccc ccacgagtg acgagccca 
cgattcgag acttgttca aaataatcc agcaacaaa 
acgaacaaa ttatgttgc tccaaaggc aggtaaaca 
acctgaaaa ctagcataa gacagcccg agatcgaat 
cagggcccc acgacgact tgaacaaca gccccaaga 
agagtttgt tttcgtttg acgtcaaag cgacaagtt 
ttgaatttt cgttccggc cccaacata ataaatttc 
gaaaacaaa acgattttc ttgagccaa ggattcagg 
aaattgtcg aaaattgcg ccgaaaatc cgaattcgt 
tgcgactag aagagtcgc taaatgtga gagatagct 
acgttagtg aagtgccga gtattcaga ttaaaatgg 
atgagtatc acagttcag atgacgagg aggagtctc 
agggtaata ataacgttg ggcttacgt tctataaag 
acttaatgg atcaggtcc ataatgttt tgaaaaaag 
tccaaaaac agtcggagt gtcaaaaca ggcctggcc 
aaactatga aagctgtga aacaagcaa ctttgcaac 
atttgaaag acattggcg tgaatttat cgccaatac 
aggaatcta gtgaggtca caagagaaa gtgattgtg 
accaaaccg atgtcagtt gtgccgtcc aacaactcc 
taatgaaat atttacctg aagataagt tggccaaag 
caataaata aacctcgtc caatcaaag acaaccaat 
tcaagccgc ccctaaaca ctgactttt cattcgcaa 
cacttcaat acatgaaaa aattttaga tgtgacagc 
gctgaaatt gcagcct
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY