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Gene information
General information
TC011165 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3407457..3420135
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011165
    iBeetle screen iB_01816

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011165 RA
      MTNVDEILN YKRKDDEDF YAILGCDEK 
      STVEQITAE YKVRALQCH PDKNDGDKE 
      AEQKFQLLK QAKEILCDP EKRANYDKW 
      RNSGISISY KQWLGMKEH VHQSMHWST 
      PKTKDRMLE TGGSSGPSS LGAGGISAA 
      QRRASEGGA NLHWGGKGT GSAWGSNAP 
      SEVVSKFRN YEI
    CDS sequence
    • >TC011165 RA
      atgacgaat gtggacgag atcctgaac tacaagcgc 
      aaggacgac gaggatttt tacgcgatt ctgggatgc 
      gatgagaaa tcgacggtg gaacaaatc acggccgaa 
      tacaaagtc cgggccttg cagtgccac cccgacaag 
      aacgatggc gataaagag gccgagcag aagttccaa 
      ctgctgaag caagctaaa gaaatcctg tgcgatcct 
      gaaaaacgt gccaactac gataaatgg cgaaacagc 
      ggcatttca ataagctac aaacagtgg ctcggtatg 
      aaagaacac gtccatcag tcgatgcac tggagcacc 
      cccaagacc aaggatcgc atgctggag accggcggc 
      tcgtcgggc ccctccagt ctgggggcg ggcgggatc 
      agcgctgcc cagagacgc gcctctgag gggggcgcc 
      aacctgcat tgggggggc aagggcacc ggatcggct 
      tggggctcc aacgccccc agtgaggtt gtcagtaaa 
      ttccgaaat tatgaaatc taa
    mRNA sequence
    • >TC011165 RA
      taattatta caaaaatgc tccattgac cacaattgc 
      aacactcct ccgagcctt tcacttgtt ggcttcgtc 
      ggagtcgag tcgaaaaat ccgcgatat cgccgagcg 
      caagtgtcg tagagcagc tctgcgttt cccgatttt 
      cccatttcc ctcagtcgt agtcgggaa agcgtggag 
      tgaagtgca aaatgacga atgtggacg agatcctga 
      actacaagc gcaaggacg acgaggatt tttacgcga 
      ttctgggat gcgatgaga aatcgacgg tggaacaaa 
      tcacggccg aatacaaag tccgggcct tgcagtgcc 
      accccgaca agaacgatg gcgataaag aggccgagc 
      agaagttcc aactgctga agcaagcta aagaaatcc 
      tgtgcgatc ctgaaaaac gtgccaact acgataaat 
      ggcgaaaca gcggcattt caataagct acaaacagt 
      ggctcggta tgaaagaac acgtccatc agtcgatgc 
      actggagca cccccaaga ccaaggatc gcatgctgg 
      agaccggcg gctcgtcgg gcccctcca gtctggggg 
      cgggcggga tcagcgctg cccagagac gcgcctctg 
      aggggggcg ccaacctgc attgggggg gcaagggca 
      ccggatcgg cttggggct ccaacgccc ccagtgagg 
      ttgtcagta aattccgaa attatgaaa tctaaagtg 
      tagatgttt cttgtgtat ttatgtgat ctttagtta 
      gacggatgt gtcactgtg ggggcttcc cccgaatcc 
      taagacctg tttgatgtc ccgaacttg actctagat 
      cgatcccgt cattgcaat gacgttcac taatcaaat 
      taaacaaaa aaacaaatt tttgcgttt attggccat 
      atttagaca atcgaaatt aggtttcgg tttggtatc 
      ttggaccaa attaatgaa gggggtttg caacttttt 
      catgaaaat ccctttata tttattgtg atgtgatat 
      tagtttttt atggagaga taaatatgc tattgcaaa 
      aatcgtgca tgtttcatt tattatgtc ttggtgtat 
      acagggtac ctgaaaact agtacatta ttgtttccg 
      ccacccttt ataccacca accgatttt gtttcaaat 
      acgtggaaa ctactcata tccctaaca tcagggtaa 
      atgcacaat tatccctac tccctttac actatatat 
      tttgataaa tgttttaaa caacatatt ttaaattaa 
      tgttatcaa tcaaatata atctattta taaatgtta 
      aatggaaat aaaaattat catgtgac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY