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Gene information
General information
TC011160 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid74: 1060806..1063236
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011160
    iBeetle screen iB_01815
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Fertility
    eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (14 dpi)
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 3

    wings size decreased - Number of animals showing the phenotype: 4

    legs sometimes not present - Number of animals showing the phenotype: 4

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011160 RA
      MRNLSLKRK LRSLYIVTA SGLGAYVAI SLYKNNEKF 
      YRNVVMPLV HLLEPEQAH RLGVIVSKY RLVPSTNYK 
      DPESLKITL FNKVFPNPI GIAAGFDKH AEAVMGLKD 
      IGFGFIEIG SVTPQPQPG NNKPRVFRL SEDLAVINR 
      YGFNSDGHE TVLHRLINL RQTKKCDTI LGVNLGKNK 
      TSDDAINDY VIGIKKLGP VADYLVINV SSPNTPGLR 
      TMQDKNVLK NLLKSAKEA RDSLPEGAR PPLLLKLAP 
      DLSYSELKD IANVVRQKD TKIDGLIIC NTTVERPQF 
      LHNDRKGET GGLSGKPLR EASTKLIAE MYKLTRGEI 
      PIIGVGGVF TGRDAYEKI KAGASVVQL YTSLVYEGP 
      PVVAKIKRE LVELLADDG FKNVSEAVG CSVK
    CDS sequence
    • >TC011160 RA
      atgaggaat ttgagttta aagaggaaa ctacgatca 
      ttgtatatt gttactgcg agcggatta ggggcatat 
      gtggctata agtctgtat aaaaacaat gaaaagttt 
      tacagaaac gttgtaatg cctttagta catttgctg 
      gagcctgag caagcccac cggctcggg gttatagtc 
      agtaaatac cgccttgtc ccctcaact aattacaaa 
      gaccccgag tctttgaaa attactctt ttcaataaa 
      gttttccca aacccaatt ggcatcgct gccggtttt 
      gacaaacac gccgaagcg gtaatgggt ttgaaagac 
      attggtttt ggtttcatt gaaataggt tcagtgaca 
      ccccagcct cagccaggc aataacaaa ccgcgagtt 
      ttccgcctg tctgaagac cttgctgtt ataaacaga 
      tacgggttc aacagcgac ggccacgag accgtttta 
      caccgactt attaatttg agacaaacc aaaaaatgt 
      gatactata ctaggggtc aatttagga aaaaacaag 
      acttcagac gacgctatc aatgactac gtcattggt 
      attaaaaaa ttagggcca gttgctgac taccttgtt 
      atcaatgtt agcagtcct aacacgccc ggcttaagg 
      acaatgcaa gacaaaaac gtactaaaa aatctatta 
      aaaagtgcc aaagaggca cgtgatagc ctccctgag 
      ggggccaga cccccacta ttacttaaa ctagcgccc 
      gacttgtcc tacagtgag ctcaaagac attgcaaat 
      gtggtgagg caaaaagac acgaaaatt gatgggctt 
      ataatttgc aatacgaca gttgaaagg ccacaattt 
      ctgcataat gacaggaaa ggggagaca ggggggctg 
      agtggcaag cctttgaga gaggcctcc actaagtta 
      atcgcagaa atgtacaaa ctgacacgg ggggaaata 
      ccgattata ggtgttggc ggtgttttt accggccga 
      gacgcctac gaaaaaatc aaagctggg gctagtgtt 
      gtacaattg tacacaagt ctggtgtat gaggggccc 
      ccggttgtt gccaaaatt aaacgggag ctagtggag 
      ttgttggcc gatgatggg tttaaaaac gtttcggag 
      gctgtgggg tgcagtgtt aaataa
    mRNA sequence
    • >TC011160 RA
      cgggcgcgc ttcaaatta cgcaaaagc gcatcgtct 
      gaactggat taatggtgt cacgttagg taacagtca 
      caattcaca attaacagc acttctaaa tatccaagg 
      gccttttgt ttaaatatt catttgcgc ttcttttgg 
      acattttgt gttcttcca tgaatatta ttaaacata 
      attccggca gcaacactt tgaagaagt aactgtcca 
      ttttatcct cttggtcaa actcagatt tcattaaat 
      caaggagta cttccttct gttgttcac taaacacga 
      taacactgt tgctacaaa acaagctct tggctgaag 
      aaatgttgt tgccacttt ggtgaaacg cccgatggc 
      gatctgaat atttgcgtc cttccataa ttgcgtcca 
      aagtgtctt tgtgtctct caaacgctt tcttcaagt 
      ccttggaag tcaggtgtt tacaatcaa gaagctcct 
      ccatgaagt tatcttctt ttagactat caatgccca 
      gatttttct ggagaatat cttcatcga aaacatcaa 
      gctgttcaa cactgcgtc tatattgat caaagttct 
      tctgatctg ttacgattt tctcaaacg agggaactg 
      acacgccgt acttttcaa acgaaaaac tcctttgca 
      aactcttct tcttcttca cggtaacat caagctgtt 
      ctggctgct tcttctgcg cccgtttcg actcacctt 
      tattctcct tattcaacc acgaagtaa ttttggggc 
      ttttgtcgc aggctggct ttcgtttac gagtcgaag 
      cggccttct ttggttcag tcggagatg ccctctttt 
      gcagttgtc tcagttttt ccatgagat tattcatgg 
      ctttgtcac cacacgatt tttggggct ccattctgg 
      aaacccgcc acttcaatt tgacgttgt caacttgac 
      gtttctaaa gtgaggtta gggtttgtt tatttttga 
      aaattcgcc tgatttttg taataaaat gaggaattt 
      gagtttaaa gaggaaact acgatcatt gtatattgt 
      tactgcgag cggattagg ggcatatgt ggctataag 
      tctgtataa aaacaatga aaagtttta cagaaacgt 
      tgtaatgcc tttagtaca tttgctgga gcctgagca 
      agcccaccg gctcggggt tatagtcag taaataccg 
      ccttgtccc ctcaactaa ttacaaaga ccccgagtc 
      tttgaaaat tactctttt caataaagt tttcccaaa 
      cccaattgg catcgctgc cggttttga caaacacgc 
      cgaagcggt aatgggttt gaaagacat tggttttgg 
      tttcattga aataggttc agtgacacc ccagcctca 
      gccaggcaa taacaaacc gcgagtttt ccgcctgtc 
      tgaagacct tgctgttat aaacagata cgggttcaa 
      cagcgacgg ccacgagac cgttttaca ccgacttat 
      taatttgag acaaaccaa aaaatgtga tactatact 
      aggggtcaa tttaggaaa aaacaagac ttcagacga 
      cgctatcaa tgactacgt cattggtat taaaaaatt 
      agggccagt tgctgacta ccttgttat caatgttag 
      cagtcctaa cacgcccgg cttaaggac aatgcaaga 
      caaaaacgt actaaaaaa tctattaaa aagtgccaa 
      agaggcacg tgatagcct ccctgaggg ggccagacc 
      cccactatt acttaaact agcgcccga cttgtccta 
      cagtgagct caaagacat tgcaaatgt ggtgaggca 
      aaaagacac gaaaattga tgggcttat aatttgcaa 
      tacgacagt tgaaaggcc acaatttct gcataatga 
      caggaaagg ggagacagg ggggctgag tggcaagcc 
      tttgagaga ggcctccac taagttaat cgcagaaat 
      gtacaaact gacacgggg ggaaatacc gattatagg 
      tgttggcgg tgtttttac cggccgaga cgcctacga 
      aaaaatcaa agctggggc tagtgttgt acaattgta 
      cacaagtct ggtgtatga ggggccccc ggttgttgc 
      caaaattaa acgggagct agtggagtt gttggccga 
      tgatgggtt taaaaacgt ttcggaggc tgtggggtg 
      cagtgttaa ataatttta ttatttgat taacttttc 
      caataaatt taatatcaa aaa