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Gene information
General information
TC011159 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3499330..3501321
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011159
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01814

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    remarks: later than schedule, adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Adult morphology
    abdominal odoriferous glands mostly colour darker - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    Analysis of larval stage 1 cuticle
    remarks - cuticles: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011159 RA
      MAPIKATSL LNLAGKALH RRQFSVSKI ASAKQMTVR 
      DALNSALDE EMTRDERVF IIGEEVAQY DGAYKVTRG 
      LWKKYGDKR VIDTPITEM GFTGIAVGA AMAGLRPVC 
      EYMTFNFAM QAIDQIINS AGKTFYMSA GRVNVPIVF 
      RGPNGAAAG VGAQHSQCY GAWYAHCPG LKVISPYNS 
      EDCKGLLKA AIRDPDPVV FLENEILYG VQYPMSDQA 
      LSNDFVLPI GKAKIERPG KHITIVAHS RAVETSLQA 
      ANELSSKGI EAEVINLRS LRPLDINTI TASVAKTNH 
      LITVEQGWP SAGIGAEIL ARIMESEAF FHLDQPAIR 
      LTGVDTPMP YTKSLEMAA LPVPKDVVE MTKKLLKVK
    CDS sequence
    • >TC011159 RA
      atggcaccg atcaaagcc actagtctt ttgaacttg 
      gccggaaaa gccctccac cggagacaa ttctcagtg 
      tcaaaaatc gcatctgcc aaacaaatg acagtcaga 
      gacgcccta aattcagcc ctagacgag gaaatgacc 
      cgcgatgag cgggttttc atcatcggc gaagaggtg 
      gcgcagtac gacggcgcc tacaaggtc acaaggggg 
      ctgtggaag aaatacggc gataagcga gtcatcgac 
      acgccaatc acagaaatg gggttcaca ggaatcgcc 
      gtgggggct gccatggcc ggcctgcgc cccgtctgc 
      gagtacatg acgttcaac ttcgccatg caagccatc 
      gaccagatc atcaactcg gcgggaaag accttctac 
      atgtccgcg ggccgcgtc aacgtcccc attgtcttc 
      cgggggccc aatggggct gccgccggc gttggcgcc 
      caacattcc cagtgctac ggcgcgtgg tacgcacac 
      tgtccaggc ctcaaggtc atctccccc tacaattcc 
      gaagactgc aaggggctc ctcaaggcc gccattcgg 
      gaccctgac cccgtggtt ttcctcgaa aacgaaatt 
      ttgtacggg gtgcagtac cccatgtca gatcaggcc 
      ctttccaac gattttgtc ctaccgata ggcaaagcg 
      aaaatcgag cgaccagga aaacacata acgattgtg 
      gcgcactcc cgggcggtt gaaacgtca cttcaagca 
      gcgaatgaa ctttcctcg aaaggaatt gaagcagaa 
      gtgatcaat ttgcgatcg ttgcgccct ctggacatc 
      aatacgata actgcgtcc gttgctaaa acgaatcac 
      ttgattacg gtggagcaa gggtggcct tcggcaggg 
      atcggggcc gagattttg gccagaatt atggaaagc 
      gaggccttt ttccactta gaccaacca gcgatcaga 
      ctgacaggc gttgacacg cccatgcca tataccaaa 
      agtctagaa atggccgcg cttccggtg cctaaagac 
      gtagtcgaa atgacgaaa aaactactg aaggttaag 
      taa
    mRNA sequence
    • >TC011159 RA
      cctagtgcc cacaacctc aattacaaa aaattgaga 
      aacctgtca aacttaacc ttcaaaaat ccgaatttt 
      atcaaaaat ctcagattt gatctctcg ctaggagca 
      tgtctccaa gatagcgtc acatgtgcg attacgtat 
      aacgaatgc tcaagagat aataaccta acaatcaac 
      accgacgtt tattcggtc ctctcattc tccttcacg 
      accttaatc accttgact gtcaaatca gaaaaatgg 
      caccgatca aagccacta gtcttttga acttggccg 
      gaaaagccc tccaccgga gacaattct cagtgtcaa 
      aaatcgcat ctgccaaac aaatgacag tcagagacg 
      ccctaaatt cagccctag acgaggaaa tgacccgcg 
      atgagcggg ttttcatca tcggcgaag aggtggcgc 
      agtacgacg gcgcctaca aggtcacaa gggggctgt 
      ggaagaaat acggcgata agcgagtca tcgacacgc 
      caatcacag aaatggggt tcacaggaa tcgccgtgg 
      gggctgcca tggccggcc tgcgccccg tctgcgagt 
      acatgacgt tcaacttcg ccatgcaag ccatcgacc 
      agatcatca actcggcgg gaaagacct tctacatgt 
      ccgcgggcc gcgtcaacg tccccattg tcttccggg 
      ggcccaatg gggctgccg ccggcgttg gcgcccaac 
      attcccagt gctacggcg cgtggtacg cacactgtc 
      caggcctca aggtcatct ccccctaca attccgaag 
      actgcaagg ggctcctca aggccgcca ttcgggacc 
      ctgaccccg tggttttcc tcgaaaacg aaattttgt 
      acggggtgc agtacccca tgtcagatc aggcccttt 
      ccaacgatt ttgtcctac cgataggca aagcgaaaa 
      tcgagcgac caggaaaac acataacga ttgtggcgc 
      actcccggg cggttgaaa cgtcacttc aagcagcga 
      atgaacttt cctcgaaag gaattgaag cagaagtga 
      tcaatttgc gatcgttgc gccctctgg acatcaata 
      cgataactg cgtccgttg ctaaaacga atcacttga 
      ttacggtgg agcaagggt ggccttcgg cagggatcg 
      gggccgaga ttttggcca gaattatgg aaagcgagg 
      cctttttcc acttagacc aaccagcga tcagactga 
      caggcgttg acacgccca tgccatata ccaaaagtc 
      tagaaatgg ccgcgcttc cggtgccta aagacgtag 
      tcgaaatga cgaaaaaac tactgaagg ttaagtaaa 
      atctcgagt aatgttcct ttgtttgaa attgaagcg 
      tcgtctgta ttgccaacg tacagttat tataattta 
      tttatttat ttctcgtcc ggctgaatt aatcaaagg 
      gtaagttta taattttta cttgtacaa actgttgta 
      aataatagt ctagtttta attggatca agtgtacat 
      tgcattttt taagccaat tttttctta tgtggccac 
      ttttacggt tgtaaattt ttgtagaca ataaatcat 
      taaggctga t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY